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Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds
2023
Beinhauerova Monika | Slana Iva
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a chronic infectious intestinal disease occurring in domestic and wild ruminants. Early diagnosis of infected herds enabling timely adoption of control measures is tremendously important in view of the fact that the disease has a significant economic impact on farmers. The aim of this study was to evaluate the possibility of rapid detection of viable MAP on small ruminant farms based on environmental sample examination using a novel phage-based test named Actiphage.
Afficher plus [+] Moins [-]First molecular detection of Brachyspira suanatina on pig farms in Poland
2023
Cybulski Piotr | Strutzberg-Minder Katrin | Michalik Edyta | Kondratiuk Radosław | Jablonski Artur
Prior to the 2000s, swine dysentery was considered to be caused only by Brachyspira hyodysenteriae with contributing commensal intestinal anaerobes. Nowadays, it is known that the disease is caused by three strongly beta-haemolytic species of the anaerobic spirochaetal genus Brachyspira, i.e. B. hyodysenteriae and newly emerged B. hampsonii and B. suanatina.
Afficher plus [+] Moins [-]Exercise-induced haematological and blood lactate changes in whippets training for lure coursing
2023
Miazga Katarzyna | Szaluś-Jordanow Olga | Czopowicz Michał | Żmigrodzka Magdalena | Witkowska-Piłaszewicz Olga | Moroz-Fik Agata | Mickiewicz Marcin | Wilczak Jacek | Cywińska Anna
Whippets are traditionally trained to compete in lure coursing. While in humans and horses, training is routinely monitored by special tests, this is not carried out in the training of whippets. The aim of this study was to check if laboratory tests designed for racehorses could be useful in monitoring whippets training for lure coursing.
Afficher plus [+] Moins [-]Comparison of various diagnostic techniques for the detection of Blastocystis spp. and its molecular characterisation in farm animals in the United Arab Emirates
2023
ElBakri Ali | Kanu Gayathri A. | Salahat Dana | Hussein Nabila | Ibrahim Zeinab | Hasan Hayder | AbuOdeh Raed
Blastocystis spp. is a common anaerobic intestinal parasite infecting humans and a diverse range of animals. The aim of the study was to compare different diagnostic methods for the detection of Blastocystis and survey the occurrence of its subtypes in farm animals, namely sheep, cows and camels, in Al-Ain, United Arab Emirates.
Afficher plus [+] Moins [-]Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle
2023
Klepp Laura I. | Colombatti María A. | Moyano Roberto D. | Romano María I. | Malovrh Tadej | Ocepek Matjaž | Blanco Federico C. | Bigi Fabiana
Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis, respectively the causative agents of bovine tuberculosis (bTB) and bovine paratuberculosis (PTB), share a high number of antigenic proteins. This characteristics makes the differential diagnosis of the diseases difficult. The interferon gamma (IFN-γ), C-X-C motif chemokine ligand 10 (CXCL10), matrix metallopeptidase 9 (MMP9), interleukin 22 (IL-22) and thrombospondin 1 (THBS1) bovine genes have already been shown to be accurate transcriptional biomarkers of bTB. In order to improve the diagnosis of bTB and PTB, in the present study we evaluated the risk of false positivity of these bTB biomarkers in cattle with PTB.
Afficher plus [+] Moins [-]The small non-coding RNA rli106 contributes to the environmental adaptation and pathogenicity of Listeria monocytogenes
2023
Guo Yun | Ji Chunhui | Wang Lixia | Ning Chengcheng | Li Na | Li Zhiyuan | Shang Yunxia | Li Yaling | Sun Yaoqiang | Huang Xiaoxing | Li Jie | Cai Xuepeng | Meng Qingling | Qiao Jun
Listeria monocytogenes (LM) is an important food-borne pathogen, and the risk of its ingestion is a serious public health issue. The better its environmental adaptation mechanisms and pathogenicity are understood, the better the risk it poses can be countered. The regulatory role of the small non-coding RNA (sRNA) rli106 in the environmental adaptation and pathogenicity of LM is still unclear and this study investigated that role through its biological function.
Afficher plus [+] Moins [-]The role of leukotriene B4 in cow metritis
2023
Wang Guo-Qing | Zheng Hao-Yue | Hou Jin-Long | Wang Cui | Sun Hui-Lian | Wang Lan
Metritis is a common postpartum disease in dairy cows. As a mast cell (MC) mediator, leukotriene B4 (LTB4) is the strongest phagocyte chemokine. It is important in inflammation for the recruitment of immune cells to resist infection. This study investigated the effect of LTB4 in metritis.
Afficher plus [+] Moins [-]Bacterial and viral rodent-borne infections on poultry farms. An attempt at a systematic review
2023
Domanska-Blicharz Katarzyna | Opolska Justyna | Lisowska Anna | Szczotka-Bochniarz Anna
Rodents are quite common at livestock production sites. Their adaptability, high reproductive capacity and omnivorousness make them apt to become a source of disease transmission to humans and animals. Rodents can serve as mechanical vectors or active shedders of many bacteria and viruses, and their transmission can occur through direct contact, or indirectly through contaminated food and water or by the arthropods which parasitise infected rodents. This review paper summarises how rodents spread infectious diseases in poultry production.
Afficher plus [+] Moins [-]Antioxidant and antimicrobial properties of an extract rich in proteins obtained from Trametes versicolor
2023
Michalak Katarzyna | Winiarczyk Stanisław | Adaszek Łukasz | Kosikowska Urszula | Andrzejczuk Sylwia | Garbacz Katarzyna | Dobrut Anna | Jarosz Łukasz | Czupryna Wiktoria | Pietras-Ożga Dorota
Bioactive proteins and peptides generated from fruit, vegetables, meat or fish have great potential as functional food or substitutes for antibiotics. In recent years it has also been demonstrated that the fungus kingdom could be a source of these compounds. The study investigated the bioactivity of an extract of the lignicolous fungus Trametes versicolor and its hydrolysate.
Afficher plus [+] Moins [-]Prevalence of the blaCTX-M and blaTEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia
2023
Faridah Hayyun Durrotul | Wibisono Freshindy Marissa | Wibisono Freshinta Jellia | Nisa Nabilatun | Fatimah Fatimah | Effendi Mustofa Helmi | Ugbo Emmanuel Nnabuike | Khairullah Aswin Rafif | Kurniawan Shendy Canadya | Silaen Otto Sahat Martua
Infections of humans and animals by multidrug resistant bacteria are increasing because of the inappropriate use of antibiotics. Disease management may be more challenging if Escherichia coli produce extended-spectrum beta-lactamase (ESBL), which could cause resistance to aztreonam and third-generation cephalosporins. This study was aimed at determining the prevalence of the blaCTX-M and blaTEM genes among ESBL-producing E. coli isolated from broiler chickens in Indonesia.
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