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Distribution of TNF receptors and TNF receptor-associated intracellular signaling factors on equine tendinocytes in vitro
2004
Hosaka, Y. (Rakuno Gakuen Univ., Ebetsu, Hokkaido (Japan)) | Sakamoto, Y. | Kirisawa, R. | Watanabe, T. | Ueda, H. | Takehana, K. | Yamaguchi, M.
Although tumor necrosis factor (TNF) alpha is an important key factor in degeneration of equine superficial digital flexor tendon (SDFT), the dynamism of TNF receptors and associated factors on tendinocytes has not been elucidated. To reveal signaling events mediated by TNF-receptors (TNF-Rs) in tendinocytes, we focused on four signaling factors, TNF-R1, TNF-R2, TNF-Rassociated factor 2 (TRAF2) and nuclear factor-kappa B (NE-KappaB) , and investigated the distribution and production of these factors. Cultured tendinocytes were obtained from SDFTS of thoroughbred horses. The tendinocytes were treated with 10ng/ ml equine TNFAlpha medium for 6 hours and then the four factors on tendinocytes were visualized by using an immunohistochemical method, and the amounts of the four factors were determined by Western blot analysis. Although TNF-R1 and TNF-R2 co-localized on the same tendinocyte, in untreated control cells (normal condition), immunoreactivity against TNF-R1 was very weak but TNF-R2 showed a strong reaction. However, TNF-R1 showed the same high level of reaction as TNF-R2 in TNFAlpha-treated cells (inflamed condition). Intense TRAF2 and NF-KappaB were detected at inflamed condition, however both factors were also detected at normal condition. The distinct distributions of the four factors under different conditions (normal and inflamed condition) in uitro not only reflect the dynamism of the cytokines but may also provide important clues for a means to prevent from occurrence of tendonitis and progress of tendon degeneration.
Afficher plus [+] Moins [-]Identification and morphological characteristics of dental neonatal line in sika deer (Cervus nippon)
2004
Iinuma, Y.M. (Hokkaido Univ., Sapporo (Japan)) | Suzuki, M. | Matsuura, Y. | Asano, M. | Onuma, M. | Ohtaishi, N.
Evidence for bovine immunodeficiency virus infection in cattle in Zambia
2004
Meas, S. (Hokkaido Univ., Sapporo (Japan)) | Nakayama, M. | Usui, T. | Nakazato, Y. | Yasuda, J. | Ohashi, K. | Onuma, M.
Reference serum protein and lipoprotein fractions of ostriches (Struthio camelus) in Turkey
2004
Polat, U. | Cetin, M. (Uludag Univ., Bursa (Turkey). Biochemistry Dept.) | Turkyilmaz, O. | Yalcin, A.
Development of a novel self-medicating applicator for control of internal and external parasites of wild and domestic animals
2004
Burridge, M.J. | Simmons, L.A. (Florida Univ., Gainesville (USA). Pathobiology Dept.) | Ahrens, E.H. | Naude, S.A.J. | Malan, F.S.
Linognathus weisseri n. sp. (Phthiraptera: Linognathidae) of impalas, Aepyceros melampus: description and biology
2004
Durden, L.A. (Georgia Southern Univ., Statesboro (USA). Biology Dept.) | Horak, I.G.
Limitation of the spread and impact of infectious coryza through the use of a continuous disinfection programme
2004
Bragg, R.R. (Free State Univ., Bloemfontein (South Africa). Microbial, Biochemical and Food Biotechnology Dept.)
Effect of diminazene block treatment on live redwater vaccine reactions
2004
Combrink, M.P. | Troskie, P.C. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.)
The testing and modification of a commercially available transport medium for the transportation of pure cultures of Haemophilus paragallinarum for serotyping
2004
Bragg, R.R. | Jansen van Rensburg, P. | Van Heerden, E. | Albertyn, J. (Free State Univ., Bloemfontein (South Africa). Microbial, Biochemical and Food Biotechnology Dept.)
Molecular epidemiology of serotype O foot-and-mouth disease virus isolated from cattle in Ethiopia between 1979-2001
2004
Sahle, M. | Venter, E.H. (Pretoria Univ., Onderstepoort (South Africa). Veterinary Tropical Diseases Dept.) | Dwarka, R.M. | Vosloo, W.
