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Determination of Tear Volume and Intraocular Pressure in Saanen Goat and Sakiz Sheep in Similar Environmental Conditions Texte intégral
2021
Kibar Kurt, Büşra | Bulut, Osman | Bozkan, Zeynep | Bilgen Şen, Zeynep | Belge, Ali
The aim of this study is to identify physiological reference values for Schirmer tear test (STT) and intraocular pressure (IOP) results, in healthy sheep and goats. The study included 56 adult Sakiz sheep and 38 Saanen goats with no ocular disease. Tear volume was measured by a sterile STT strip. IOP was measured with Tono-Vet. IOP and STT readings were carried out in July, at 12.30-14.30 hrs. Measurements were write down in similar conditions in sheep and goats (at 41.8°C-30% humidity and 40.5°C and 32% humidity, respectively). No statistically significant difference was determined between the mean IOP and STT values of the left eye and the right eye in the statistical analysis of goats and sheep (P>0.05). The mean STT values were 10.6±3.03 mm/min in Saanen goats and 11.8±3.35 mm/min in Sakiz sheep. The mean IOP values were 16.5±5.46 mmHg and 17.7±4.57 mmHg for Saanen goats and Sakiz sheep, respectively. A statistically significant difference was determined in the STT (P<0.05) and IOP (P<0.01) values between goats and sheep. In the literature review for reference IOP and STT levels, there is no report for Sakız sheep. Reference values of STT and IOP in summer conditions in Saanen goats and Sakiz sheep were revealed and provide more information for experimental and clinical studies.
Afficher plus [+] Moins [-]Direct Detection of Cryptosporidium spp. In Cattle In Karbala Province and its Environs, Iraq Texte intégral
2021
Alali, Firas | Jawad, Marwa | kh.k.Al-khayat, Kadhim
Gastrointestinal diseases are caused by many of the apicomplexan protozoan parasites and cause significant clinical diseases. Cryptosporidiosis is one of the most important diseases of young ruminant livestock, particularly neonatal calves. This study investigates the prevalence of Cryptosporidium infection in Karbala farm cattle. Totally, 1360 fecal samples were collected from cows and calves between (1 week to 5years old) from both genders, from different regions of Karbala. Different fecal examination methods (direct fecal smear, fecal flotation with saturated salt solution and faecal smear stained by modified Ziehl-Neelsen were performed to determine Cryptosporidium spp. oocysts and other mixed parasites in the fecal samples. The results showed that 29.4% of the fecal samples were detected positive for cryptosporidiosis (400/1360). The highest infection rate appeared (41.9%) in the age group from 3-6 months old, and less rate was (15.1%) in 2 years (P≤0.05). Additionally, there was no significant variation found in infection rates between males (33.8%) and females (25.6%). Mixed infections have been reported with Eimeria spp. oocysts (7.3%), Giardia lamblia cysts (1.4%) and Trichostrongylus spp. eggs (5.1%) (P≤0.05). The results indicated the requirement for the eradication of the gastrointestinal parasites by deworming and a good management system in the sampled region.
Afficher plus [+] Moins [-]Alterations in Gut Integrity Due to Heat Stress Among Dairy Cattle of Aydin City: Analytical Interpretation of Zonulin Levels Within Repetitive Measurements Texte intégral
2021
Alıc Ural, Deniz | Ural, Kerem | Erdogan, Hasan | Erdogan, Songul
Animal health and production are impaired with heat stress inducing dysfunction of intestinal barrier. In this study, it was aimed to determine that the effect of heat stress on gut health via circulating zonulin concentrations. Holstein dairy cows (n=7, age of 2-5 years) were housed in Aydin municipality with higher temperatures in summer. Blood sample for serum zonulin levels were collected at 12 pm and 00 am with temperature records of 44°C and 31°C respectively. Serum zonulin levels were found to be statistically significant (p=0,012) at night in contrast to daylight analytes. With this study it was appeared that heat stress had a negatively impact on intestinal integrity among cows.
Afficher plus [+] Moins [-]Investigation of the prevalence of Mycoplasma ovipneumoniae in Southern Xinjiang, China Texte intégral
2021
Zhao Jin-yu | Du Yi-zhou | Song Ya-ping | Zhou Peng | Chu Yue-feng | Wu Jun-yuan
It is very important to monitor the infection of Mycoplasma ovipneumoniae as a potential threat to the sheep industry. Southern Xinjiang is a major sheep breeding base in China, however, there is no relevant information concerning the infection of the region’s ovine stock with this bacteria at present. This study aimed to address this knowledge gap.
Afficher plus [+] Moins [-]High-throughput sequencing in vaccine research Texte intégral
2021
Pasik Katarzyna | Domańska-Blicharz Katarzyna
The world of vaccines has changed tremendously since the time of Louis Pasteur. In the present day, it is regarded as vaccinology, a discipline which includes not only the knowledge of vaccine production, strategies for its delivery and influence on the clinical course of disease and the response of the host immune system but also regulatory, ethical, economic and ecological aspects of their use. A hundred years after Pasteur created the first vaccine, there was another scientific breakthrough of great importance in this field, i. e. Sanger sequencing. Progress in genome sequencing and other molecular techniques over the intervening 40 years has been enormous. High-throughput sequencing (HTS) platforms and bioinformatics tools are becoming widely available, falling in cost, and results are achieved very quickly. They enable the construction of modern vaccines, as well as the assessment of their safety, effectiveness and impact on the host organism and the environment. These techniques can also provide a tool for quality control of vaccines. Unprecedented possibilities are opened up by the HTS technique, but limiting factors on its implementation have to be contended with such as lack of reference materials and problems with method optimisation or validation. In the face of the current COVID-19 pandemic, a significant role is allotted to this sequencing technique while an effective vaccine against the disease caused by SARS-CoV-2 is sough.
Afficher plus [+] Moins [-]Assessment of the usefulness of image reconstruction in the oblique and double-oblique sagittal planes for magnetic resonance imaging of the canine cranial cruciate ligament Texte intégral
2021
Przeworski Adam | Adamiak Zbigniew | Nowicki Michał | Mieszkowska Marta | Tobolska Angelika | Głodek Joanna
The aim of the study was to determine the quality and significance of the magnetic resonance image of the canine knee after reconstruction in the oblique and double-oblique sagittal plane. This reconstruction and 3D images are rarely used in common protocols due to the longer study time they require. The study aimed to demonstrate significance for such diagnostic images in specific sequences in order to stimulate consideration of their more frequent use in diagnosis of diseases of the cruciate ligament in dogs.
Afficher plus [+] Moins [-]First report of multiple drug-resistant gastrointestinal nematodes of sheep in Arbat District, Sulaymaniyah, Iraq detected by in vivo and in vitro methods Texte intégral
2021
Dyary Hiewa Othman | Banaz Hamasalih Qadir
The control of gastrointestinal nematodes (GINs) in small ruminants is principally dependent on anthelmintic therapy, which encounters the rising problem of anthelmintic resistance (AR) development. Veterinarians reported anthelmintic failure in several sheep farms in Arbat District, Sulaymaniyah, northern Iraq, which called for a systematic study about the efficacy of three commonly used drugs: albendazole, ivermectin, and levamisole.
Afficher plus [+] Moins [-]Studies on the inhibitory properties of leaf ethanolic extracts obtained from Ficus (Moraceae) species against Aeromonas spp. strains Texte intégral
2021
Pękala-Safińska Agnieszka | Tkachenko Halyna | Kurhaluk Natalia | Buyun Lyudmyla | Osadowski Zbigniew | Honcharenko Vitaliy | Prokopiv Andriy
The most frequently isolated bacteria in Polish aquaculture are of the Aeromonas genus; also pathogenic to human fish consumers, they cause substantial economic losses, and require antibiotic therapy to treat. Antibiotic residues in animal-derived food provoke concern. The aim of the study was to appraise the antimicrobial activity of ethanolic extracts of Ficus plant species against Aeromonas strains.
Afficher plus [+] Moins [-]Seroprevalence of feline foamy virus in domestic cats in Poland Texte intégral
2021
Materniak-Kornas, Magdalena | Frymus, Tadeusz | Löchelt, Martin | Kuźmak, Jacek
Seroprevalence of feline foamy virus in domestic cats in Poland Texte intégral
2021
Materniak-Kornas, Magdalena | Frymus, Tadeusz | Löchelt, Martin | Kuźmak, Jacek
Feline foamy virus (FFVfca) is widespread and its prevalence in naturally infected domestic cats ranges between 30% and 80% worldwide. The infection is persistent, with a sustained antibody response in FFVfca-positive cats; however to date, no defined disease or clinical symptoms have been proved to be associated with it. The goal of the presented study was to determine the prevalence of FFVfca infection in domestic cats in Poland. A total of 223 serum samples collected from domestic cats were tested with a glutathione S-transferase capture ELISA test to detect antibodies specific to capsid (Gag), accessory (Bet) and envelope (Env) FFVfca antigens. A Western blot test was used to confirm the ELISA results. The cut-off value for the Gag antigen was established by calculation and evaluation with the immunoblotting assay. The cut-off values for Bet and Env were calculated from the reactivity of Gag-negative samples. The sera of 99 cats (44%) showed reactivity to Gag, those of 80 did so (35.9 %) to Bet, while only 56 samples (25%) were reactive to Env. Only 51 (22.9%) sera were positive for all antigens. The main diagnostic antigen was selected to be Gag. A statistically significant association was found between FFVfca status and the age of the cat. This study proved the high seroprevalence of FFVfca in domestic cats in Poland for the first time and confirmed that adult cats are at higher FFVfca infection risk than preadult cats. Its results correspond to those reported from other countries.
Afficher plus [+] Moins [-]Seroprevalence of feline foamy virus in domestic cats in Poland Texte intégral
2021
Materniak-Kornas Magdalena | Frymus Tadeusz | Löchelt Martin | Kuźmak Jacek
Feline foamy virus (FFVfca) is widespread and its prevalence in naturally infected domestic cats ranges between 30% and 80% worldwide. The infection is persistent, with a sustained antibody response in FFVfca-positive cats; however to date, no defined disease or clinical symptoms have been proved to be associated with it. The goal of the presented study was to determine the prevalence of FFVfca infection in domestic cats in Poland.
Afficher plus [+] Moins [-]Characterisation of a new molecule based on two E2 sequences from bovine viral diarrhoea-mucosal disease virus fused to the human immunoglobulin Fc fragment Texte intégral
2021
González Pose, Alaín | Montesino Seguí, Raquel | Maura Pérez, Rafael | Hugues Salazar, Florence | Cabezas Ávila, Ignacio | Altamirano Gómez, Claudia | Sánchez Ramos, Oliberto | Roberto Toledo, Jorge
Characterisation of a new molecule based on two E2 sequences from bovine viral diarrhoea-mucosal disease virus fused to the human immunoglobulin Fc fragment Texte intégral
2021
González Pose, Alaín | Montesino Seguí, Raquel | Maura Pérez, Rafael | Hugues Salazar, Florence | Cabezas Ávila, Ignacio | Altamirano Gómez, Claudia | Sánchez Ramos, Oliberto | Roberto Toledo, Jorge
Proper conformational arrangement of the E2 molecules of bovine viral diarrhoea-mucosal disease virus (BVD-MDV) is crucial to obtain an effective recombinant vaccine candidate against the disease. In this study, we characterised a new molecule composed of two distinct sequences of the E2 glycoprotein of BVD-MDV and the Fc fragment of human immunoglobulin (BVDE2Fc). The chimaeric protein was expressed in mammalian cell lines of different species by adenoviral transduction and purified by immobilised metal-affinity chromatography. The N-glycans were profiled by HPLC, and the BVDE2Fc immunogenicity was assessed in male mice. The antigen-antibody reactions were evaluated by ELISA. The MDBK cell line was selected from among five for the final production of BVDE2Fc. After purification to over 90%, the N-glycan profile showed neutral and complex oligosaccharides. The mouse immunisation induced a strong humoral response, which produced antibodies able to attach to conformational epitopes on E2 molecules, while the Fc fragment barely contributed to the immune response. Additionally, BVDE2Fc attached to antibodies from bovine sera positive to distinct BVD-MDV subtypes, whereas the loss of BVDE2Fc structure during the deglycosylation process considerably diminished those interactions. These results demonstrate that the structure of E2 molecules arranged in tandem and attached to an Fc fragment could represent a viable design for future vaccine candidates against BVD-MD.
Afficher plus [+] Moins [-]Characterisation of a new molecule based on two E2 sequences from bovine viral diarrhoea-mucosal disease virus fused to the human immunoglobulin Fc fragment Texte intégral
2021
González Pose Alaín | Montesino Seguí Raquel | Maura Pérez Rafael | Hugues Salazar Florence | Cabezas Ávila Ignacio | Altamirano Gómez Claudia | Sánchez Ramos Oliberto | Roberto Toledo Jorge
Proper conformational arrangement of the E2 molecules of bovine viral diarrhoea-mucosal disease virus (BVD-MDV) is crucial to obtain an effective recombinant vaccine candidate against the disease. In this study, we characterised a new molecule composed of two distinct sequences of the E2 glycoprotein of BVD-MDV and the Fc fragment of human immunoglobulin (BVDE2Fc).
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