This study aims to determine of reductase and catalase enzymes in pasteurized goat's milk sold in Banda Aceh. The sample used was pasteurized goat's milk which was sold in Banda Aceh as many as 30 samples were taken by census. The research method used is a survey study method with a cross-sectional approach. Sample testing using reductase test and catalase test. The results showed that the reductase time of 30 samples of pasteurized goat's milk averaged 3-5 hours and the catalase number of 30 samples of pasteurized goat's milk was below 3 ml. Based on the results, it shows that there are reductase enzymes and catalase enzymes in goat's milk and have good quality in Banda Aceh.

This study aims to test the samples of abdominal fluid from cats suspected of having FIP by using vinegar as a substitute for acetic acid in the rival method. Feline Infectious Peritonitis (FIP) is a viral disease that is not zoonotic, but is very fatal with an infectious prognosis in various types of cats and it’s transmitted orally. This study aims to determine the positive or negative results of FIP infection in cats. The diagnosis of FIP can be seen through clinical symptoms such as lack of appetite, diarrhea, weakness, dehydration (reaching 8%), Body Condition Score (BCS) 2, enlarged abdomen, and ultrasound results showing ascites and swollen kidneys. This study uses vinegar as a substitute for acetic acid in the rival method. The test was carried out experimentally in a laboratory. The research data were analyzed descriptively, the results of a positive FIP reaction were indicated by the presence of a cloudy jellyfish-shaped precipitate in the test tube.

Cytauxzoon felis is a hemoparasite capable of infecting domestic and wild cats. Studies suggest that wild cats are the main reservoirs of the protozoan, and transmission occurs through the bite of an infected tick. Hemotropic mycoplasmas are Gram-negative bacteria responsible for severe hemolytic anemia. The feline immunodeficiency virus (FIV) is a retrovirus capable of generating immunosuppression in the host and persistent infection. The present work describes a case of co infection of Cytauxzoon felis, Mycoplasma haemofelis, and feline immunodeficiency virus (FIV) in a cat. A feline from the rural area was admitted to the Veterinary Hospital of Uberlândia - MG of the Federal University of Uberlândia. Fresh whole blood samples were collected for blood count, enzymatic analysis, DNA extraction, real-time PCR for the detection of Cytauxzoon felis and Mycoplasma haemofelis, and the Point of Care ELISA test for Feline Leukemia Virus (FeLV) antigens and antibodies to feline immunodeficiency virus (FIV). Piroplasms compatible with Cytauxzoon spp. were observed in the smear, and the tests performed were positive for Cytauxzoon felis, Mycoplasma haemofelis, and FIV.

Leptospirosis is a zoonotic disease in the tropical zone with a broad and endemic distribution. The condition is complex, where different host factors, serovar type, reservoirs, environment, and agricultural practices intervene. In cattle, the disease causes significant damage to the livestock sector, and the surrounding conditions can influence its dynamics. This study aimed to determine the seroprevalence of anti-Leptospira spp. antibodies and the risk factors in cattle in the tropical savannah from eastern Colombia. Blood was taken from the coccygeal veins of 1,000 animals. The MAT (Microagglutination) technique was used for the identification of the serogroup Autumnalis, Bataviae, Bratislava, Canicola, Copenhagen, Cynopteri, Grippotyphosa, Sejroe, Mini, Pomona, Shermani, Tarassovi, and Celledoni. Studies of risk factors were carried out according to the serovar through the calculation of X2 and OR. Seroprevalence of Leptospira spp. was 34.2% with 92.3% of herds. Pomona had the highest frequency of 7.9%, followed by Hardjo prajit with 7.0% and Grippotyphosa with 6.2%. Antibody agglutination with more than one serovar was seen in 102 (29.8%) samples. The serogroup with the highest correlations were Pomona and Hardjo prajit.

Both pregnancy and obesity can influence significant changes in the immune system. On this basis, the present study proposes to evaluate the humoral immune response of overweight pregnant mares in response to a commercial vaccine. Thirty pregnant Crioulo mares were separated according to body condition score (BCS) into overweight (BCS≥7/9) or lean-control (BCS= 5-6/9). In each group, the animals were subdivided into vaccinated and controls. The mares were vaccinated against EHV-1 in two doses spaced 21 days apart and had their blood collected monthly, for five months, for antibody evaluation. Both vaccinated groups had an increase in specific neutralizing antibodies after the vaccine. However, after the second dose, there was no increase in antibodies in any of the groups. Vaccinated overweight and lean-control mares did not differ at any time point. Therefore, this study demonstrated that obesity does not influence the humoral immune response in pregnant Crioulo mares.

Studies on the interactions between the intestinal microbiome and its host have strengthened in the last decade. However, publications on this topic in dogs still need to be made available, reinforcing the need for new studies and literary data for consultation. Given this, this review aims to describe the intestinal microbiome and its interactions with the canine host, which can contribute to both health and morbid conditions in these animals. The definition of microbiome encompasses the collective genome of all microorganisms that live in a defined habitat (intestine). It is known that the dog’s intestinal microbiota is varied, composed of bacteria, archaea, viruses, fungi, and protozoa. Under normal conditions, there is commensalism between some of these microorganisms and the host, which promotes critical physiological relationships and interactions that contribute to homeostasis and the consequent health of the animal. With this in mind, it is expected that the disturbances associated with the microbiome will result in imbalances in this commensal relationship and thus precipitate the development of diseases and aggravation of other diseases, thus characterizing intestinal dysbiosis.

The present study aimed at assessing the effects of combining 20 mg/kg S(+) ketamine with 25 μg/kg dexmedetomidine and 0.4 mg/kg butorphanol on the physiological parameters and anesthetic recovery time and score of eight captive scarlet macaw (Ara macao) specimens. These specimens were captured at the Marabá Zoobotanic Foundation (Fundação Zoobotânica de Marabá), Pará, using butterfly and mist nets, and subsequently subjected to the proposed protocol. The following physiological parameters were evaluated: heart rate (HR), respiratory rate (RR), saturation of peripheral oxygen (SpO2), body temperature (BT), and non-invasive blood pressure 5 min after drug administration (M0) and every 10 min thereafter (M1‒M5), with a total of 55 min of analysis of anesthetic effects. Glycemia was measured 5 min after drug administration and every 30 min thereafter. Anesthetic induction and recovery times were also determined. Among the parameters evaluated in this study, both HR and BT significantly decreased throughout the anesthetic period, with the lowest levels at 55 min after drug administration (M5). In contrast, RR did not significantly differ, and all animals remained stable, maintaining an RR close to a mean of 20 ± 8 cpm. Throughout the anesthetic period, SpO2 was 92 ± 5%, with no significant difference. The birds remained under spontaneous ventilation and without oxygen supplementation. Systolic, diastolic, and mean blood pressures remained stable, with no significant differences in any of these measurements. At M0 and M3, the glycemia decreased slightly, albeit with no significant difference justifying an adverse effect or even hypoglycemia. The anesthetic induction time, from M0 to decubitus, was 2.4 ± 0.7 min. The anesthetic recovery time, from M0 to effortless bipedal position and adequate phalangeal flexion, was 99.3 ± 32.4 min. The sedation was assessed as intense, and the anesthetic recovery was rated excellent in 62.5% and good in 37.5% of the animals.

Wild animals can be natural reservoirs of different microorganisms, essential for monitoring these pathogens for the generation of knowledge and creation of tools aimed at programs for the prevention and control of infectious diseases, including zoonoses. The objective was to report the fungal diversity in the skin of pacas in captivity in Acre, Western Amazon, Brazil. Twenty-six animals were evaluated, from which skin samples were collected by superficial scraping, hair avulsion, and sterile plastic brush. The samples were seeded on Mycosel agar, and the phenotypic characteristics of the colonies were analyzed. In 80.8% of the samples, different fungi were isolated, from the genera Candida, Microsporum, and Trichophyton, among others. This is the first description of the identification of fungi in the skin of pacas and suggests that these animals can be considered essential reservoirs of saprophytic or pathogenic microorganisms with zoonotic potential in the Western Amazon.

Infectious diseases caused by microorganisms are still a problem in Indonesia, both humans and animals. The problem of bacterial resistance to antibiotics has an impact on increasing morbidity, mortality, and health care. The problem of bacterial resistance to antibiotics has also been found in livestock, which can threaten human health. This situation has led many researchers to search for new bioactive compounds as alternative antibiotic candidates to fight multidrug resistant bacteria such as Mycobacterium tuberculosis, Staphylococcus spp., and Streptococcus spp. This study aimed to discuss and determine the development of endophytic bacteria isolated from several plants so we can know the role of endophytic bacteria in producing bioactive compounds, especially for application in veterinary medicine. Endophytic bacteria provide great potential to produce various new bioactive compounds obtained from secondary metabolite compounds from their host. Plants are one of the hosts for endophytic bacteria. Bioactive compounds from endophytic bacteria are widely used in health and veterinary applications as antibacterial, antiviral, anti-inflammatory, and antioxidant. This shows that endophytic bacteria can be a promising source of new antibiotics to overcome the problem of pathogenic bacterial resistance and disease in the world of animal health which also threatens human health. This review discusses some aspects in the phytochemistry of endophytic bacteria producing bioactive compounds and its application in veterinary medicine.

The gut microbiota has a major contribution in human physiology and influences disease pathogenesis, including in tuberculosis (TB) lung infection. Gut-lung axis has demonstrated the interplay of these two organs, mediated by metabolites produced by the gut microbes or derived from host molecules transformation. Tryptophan (Trp) is one of the essential aromatic amino acids catabolized as kynurenine, serotonin (5-hydroxytryptamine), and indole derivatives, including indole propionic acid (IPA), via 3 pathways. The latter was microbiota-derived Trp catabolism, which has known to have an immunomodulatory role, as ligands for Aryl hydrocarbon Receptor (AhR). Intriguingly, Mycobacterium tuberculosis required Trp as a nitrogen source, especially in CD4+ T cells-generated stress, to survive in the phagosome of macrophage and to cause disease. Recently, IPA is identified as a new anti-mycobacterial compound, which is specific and has broad spectrum of anti-mycobacterial activity. The structural similarity of this gut microbiota-derived metabolite and Trp allows IPA to inhibit the TrpE anthranilate synthase in Trp biosynthesis pathway in Mtb. In this review, we summarize findings from recent work by focusing on the role of Trp metabolites in host cells in TB infection.  A better understanding of this chemical signal could potentially serve as a novel strategy for managing this chronic inflammatory disease.