O linfoma é uma malignidade linfoide muito comum nos cães, mas o envolvimento cardíaco é raro. O linfoma cardíaco primário é definido como linfoma que envolve o coração, o pericárdio ou ambos, sendo classificado como forma anatômica extranodal. O presente relato descreve os casos de três animais com linfoma cardíaco, que apresentaram efusão pericárdica moderada a importante e tamponamento cardíaco, sendo a análise citológica da efusão consistente com o diagnóstico de linfoma cardíaco nos três animais. A avaliação anatomopatológica do tumor extraído do coração de um dos animais, após eutanásia, foi compatível com linfoma cardíaco. Os outros dois animais foram tratados com protocolo quimioterápico segundo Madison-Wisconsin e apresentaram sobrevida entre 6 a 8 meses. Por meio deste relato, ressalta-se a importância da citologia do líquido das efusões pericárdicas, para o diagnóstico e adequado tratamento e acompanhamento dos tumores cardíacos de cães e gatos. | Lymphoma is a very common lymphoid malignancy in dogs, but cardiac involvement is rare. Primary cardiac lymphoma is defined as lymphoma involving the heart, the pericardium, or both, and is classified as extranodal anatomical form. The present report describes the cases of three animals with cardiac lymphoma. All cases presented moderate to severe pericardial effusion and cardiac tamponade. The cytological analysis of the effusion was consistent with the diagnosis of cardiac lymphoma. The anatomopathological evaluation of the tumor extracted from the heart of one of the animals, after euthanasia, was consistent with cardiac lymphoma. The other two animals were treated with Madison-Wisconsin chemotherapy protocol and survived between 6 to 8 months. This report emphasizes the importance of pericardial effusion cytology for the diagnosis, appropriate treatment approach and monitoring of cardiac tumors in dogs and cats.

Lymphoma is a very common lymphoid malignancy in dogs, but cardiac involvement is rare. Primary cardiac lymphoma is defined as lymphoma involving the heart, the pericardium, or both, and is classified as extranodal anatomical form. The present report describes the cases of three animals with cardiac lymphoma. All cases presented moderate to severe pericardial effusion and cardiac tamponade. The cytological analysis of the effusion was consistent with the diagnosis of cardiac lymphoma. The anatomopathological evaluation of the tumor extracted from the heart of one of the animals, after euthanasia, was consistent with cardiac lymphoma. The other two animals were treated with Madison-Wisconsin chemotherapy protocol and survived between 6 to 8 months. This report emphasizes the importance of pericardial effusion cytology for the diagnosis, appropriate treatment approach and monitoring of cardiac tumors in dogs and cats.

A região central do Brasil é caracterizada como uma área endêmica para erliquiose canina. Devido a isso, o presente estudo objetivou determinar a prevalência de infecção em cães e seus carrapatos R. sanguineus, coletados a partir do mesmo ambiente. As amostras de soro e DNA genômico de sangue de 20 cães e 299 carrapatos foram testadas por RIFI e PCR a fim de detectar anticorpos e DNA de Ehrlichia canis. Do total, 9 (45%) eram soropositivos para E. canis com títulos variando de 80 a 10240 e 6 cães (30%) positivos para Ehrlichia spp. por PCR. Cinco carrapatos de vida livre (2,89%; intervalo de confiança 95%: 0,94-6,62%), e 6 carrapatos fixados em cães (4,76%; IC 95%: 1,77-10,0%) foram positivos. A taxa de infecção foi semelhante entre ambos os grupos (P=0.395). As sequências parciais do gene dsb de 2 amostras de carrapatos foram idênticas entre si e 100% (350/350) idênticas à E. canis. Apesar das altas taxas sorológicas e moleculares de erliquiose canina em Cuiabá, a prevalência de carrapatos infectados foi menor que o encontrado emcães. Por outro lado, os carrapatos adultos podem permanecer infectados por tempo suficiente para garantir a infestação e infecção aos cães susceptíveis. | The central region of Brazil is known to be an endemic area for canine ehrlichiosis. Therefore, this study aims to determine the prevalence rates of E. canis infection in dogs and in Rhipicephalus sanguineus ticks collected from the dogs and their home environments. Serum samples and genomic DNA from the blood of 20 dogs and 299 ticks were analyzed by IFA and PCR assays in order to detect Ehrlichia canis antibodies and DNA. Nine (45%) of the 20 dogs were seropositive for E. canis, with titers ranging from 80 to 10240, and 6 dogs (30%) were positive for Ehrlichia spp. by PCR. Five free-living ticks were positive (2.89%, 95% confidence interval: 0.94-6.62%), as were six ticks attached to dogs (4.76%; 95% CI: 1.77-10.0%). The two groups showed a similar infection rate (P=0.395). Partial dsb DNA sequences of two samples from ticks were identical to each other and 100% (350/350 nucleotides) were identical to E. canis. Despite the high serological and molecular rates of canine ehrlichiosis in Cuiabá, the prevalence among infected ticks was lower than that found among dogs. However, adult ticks may remain infective much longer to ensure their infestation and infection of susceptible dogs.

The central region of Brazil is known to be an endemic area for canine ehrlichiosis. Therefore, this study aims to determine the prevalence rates of E. canis infection in dogs and in Rhipicephalus sanguineus ticks collected from the dogs and their home environments. Serum samples and genomic DNA from the blood of 20 dogs and 299 ticks were analyzed by IFA and PCR assays in order to detect Ehrlichia canis antibodies and DNA. Nine (45%) of the 20 dogs were seropositive for E. canis, with titers ranging from 80 to 10240, and 6 dogs (30%) were positive for Ehrlichia spp. by PCR. Five free-living ticks were positive (2.89%, 95% confidence interval: 0.94-6.62%), as were six ticks attached to dogs (4.76%; 95% CI: 1.77-10.0%). The two groups showed a similar infection rate (P=0.395). Partial dsb DNA sequences of two samples from ticks were identical to each other and 100% (350/350 nucleotides) were identical to E. canis. Despite the high serological and molecular rates of canine ehrlichiosis in Cuiabá, the prevalence among infected ticks was lower than that found among dogs. However, adult ticks may remain infective much longer to ensure their infestation and infection of susceptible dogs.

O presente estudo avaliou vacas Brangus tratadas com doses únicas de hormônio folículo estimulante (FSH) submetidas a aspiração folicular após vinte e quatro horas, para avaliação da recuperação oocitária, fertilização in vitro e taxa de prenhez. Folículos superiores a três milímetros de diâmetro foram aspirados, 200 mg de FSH foram administrados dois dias depois e uma nova aspiração folicular foi realizada 24 horas após. Esses métodos foram efetivados três vezes a cada três dias. No controle, as aspirações foliculares ocorreram em intervalos de uma semana sem administração de FSH. Os oócitos aspirados foram avaliados, submetidos à fertilização in vitro e os embriões foram transferidos em receptoras. A recuperação média dos oócitos foi superior (p<0,05) nas vacas controle (12,4±1,8) do que nas vacas tratadas (9,4±1,3). Não houve diferença (p>0,05) na porcentagem média de oócitos viáveis (52,0±3,9 e 62,7±4,7%) ou na porcentagem média de embriões (41,4±4,8 e 41,5±4,2%) entre vacas controle e vacas tratadas, respectivamente. A porcentagem média de prenhez não diferiu (p>0,05) para as vacas controle (43,8±2,7%) e as tratadas (40,9±6,8%). Em conclusão, o tratamento com FSH não melhorou a recuperação de oócitos, a fertilização in vitro e o percentual de prenhez. No entanto, existe a possibilidade de várias aspirações foliculares consecutivas a cada três dias, concentrando a fertilização in vitro e o percentual de prenhez. | The present study evaluated Brangus cows treated with single doses of follicle stimulating hormone (FSH) subjected to follicular aspiration after 24 h to assess oocyte recovery, in vitro fertilization and pregnancy rate. Follicles exceeding 3 millimeters in diameter were aspirated, 200 mg of FSH was administered 2 days later, and a new ovum pickup was performed 24 h afterward. These methods were performed 3 times every 3 days. In control, follicular aspirations occurred at intervals of 1-week without FSH administration o. The aspirated oocytes were evaluated, submitted to in vitro fertilization and the embryos were transferred to the recipients. The average recovery of oocytes was higher (p<0.05) in control cows (12.4±1.8) than in treated cows (9.4±1.3). There was no difference (p>0.05) in the mean percentage of viable oocytes (52.0±3.9 and 62.7±4.7%) or the mean percentage of embryos (41.4±4.8 and 41.5±4.2%) among control and treated cows, respectively. The mean percentage of pregnancy did not differ (p>0.05) for control cows (43.8±2.7%), and treated cows (40.9±6.8%). In conclusion, FSH treatment did not improve oocyte recovery, in vitro fertilization, and pregnancy percentage. However, there is possibility of several consecutive ovum pickup every t3 days, concentrating the in vitro fertilization and the pregnancy percentage.

The present study evaluated Brangus cows treated with single doses of follicle stimulating hormone (FSH) subjected to follicular aspiration after 24 h to assess oocyte recovery, in vitro fertilization and pregnancy rate. Follicles exceeding 3 millimeters in diameter were aspirated, 200 mg of FSH was administered 2 days later, and a new ovum pickup was performed 24 h afterward. These methods were performed 3 times every 3 days. In control, follicular aspirations occurred at intervals of 1-week without FSH administration o. The aspirated oocytes were evaluated, submitted to in vitro fertilization and the embryos were transferred to the recipients. The average recovery of oocytes was higher (p<0.05) in control cows (12.4±1.8) than in treated cows (9.4±1.3). There was no difference (p>0.05) in the mean percentage of viable oocytes (52.0±3.9 and 62.7±4.7%) or the mean percentage of embryos (41.4±4.8 and 41.5±4.2%) among control and treated cows, respectively. The mean percentage of pregnancy did not differ (p>0.05) for control cows (43.8±2.7%), and treated cows (40.9±6.8%). In conclusion, FSH treatment did not improve oocyte recovery, in vitro fertilization, and pregnancy percentage. However, there is possibility of several consecutive ovum pickup every t3 days, concentrating the in vitro fertilization and the pregnancy percentage.

Este estudo avaliou a presença de anticorpos contra N. caninum e T. gondii em 320 cães domésticos das áreas urbana e rural do município de Poconé, no estado do Mato Grosso, região pantaneira do Pantanal. A Reação de Imunofuorescência Indireta (RIFI) foi utilizada para detectar anticorpos anti-N. caninum (ponto de corte igual a 50) e anticorpos anti-T. gondii (ponto de corte igual a 16) utilizando as cepas NC-Bahia e RH como antígenos, respectivamente. Anticorpos anti-N. caninum foram detectados em 69 (21,56%; IC 95%: 17,27%-26,56%) cães, dos quais 31 (44,93%) eram da área urbana e 38 (55,07%) eram da área rural e os títulos variaram entre 50 a 3200. Em relação aos anticorpos contra T. gondii 132 (41,25%; IC&nbsp;95%: 35,84%-46,87%) cães foram sororeagentes sendo que 58 (43,94%) eram da área urbana e 74 (56,06%) da área rural e os títulos variaram entre 16 a 8192. Um total de 33 (10,31%) (12 cães urbanos e 21 cães rurais) cães reagiram para ambos os agentes testados pela RIFI (P &lt;0,05). Este estudo mostrou uma maior ocorrência de anticorpos contra T. gondii em relação a N. caninum nos cães avaliados, mostrando que na região do Pantanal há circulação desses importantes protozoários. Essa informação é relevante dada sua importância para a saúde animal e pública. | Tis study evaluated the presence of Neospora caninum and Toxoplasma gondii antibodies in 320 domestic dogs living in urban and rural areas of the municipality of Poconé, located in the Pantanal wetlands of the state of Mato Grosso, Brazil. Anti-N. caninum (cut-of point 50) and anti-T. gondii (cut-of point 16) antibodies were detected by means of immunofuorescence assays (IFA), using NC-Bahia and RH strains as antigens, respectively. Anti-N. caninum antibodies were detected in 69 (21.56%; 95% CI: 17.27%-26.56%) dogs, 31 (44.93%) of which lived in urban areas and 38 (55.07%) in rural areas, and endpoint titers ranged from 50 to 3200. Anti-T. gondii antibodies were found in 132 (41.25%; 95% CI: 35.84%-46.87%) dogs, 58 (43.94%) from urban areas and 74 (56.06%) from rural areas, and endpoint titers ranged from 16 to 8192. A total of 33 dogs (10.3%) (12 urban and 21 rural animals) reacted to both agents (P &lt;0.05). Tis study showed a higher prevalence of anti-T. gondii compared to anti-N. caninum antibodies in the dogs living in the Pantanal region, suggesting that both protozoans circulate in the studied region. Tis information is relevant, in view of its implications for animal and public health.

Tis study evaluated the presence of Neospora caninum and Toxoplasma gondii antibodies in 320 domestic dogs living in urban and rural areas of the municipality of Poconé, located in the Pantanal wetlands of the state of Mato Grosso, Brazil. Anti-N. caninum (cut-of point 50) and anti-T. gondii (cut-of point 16) antibodies were detected by means of immunofuorescence assays (IFA), using NC-Bahia and RH strains as antigens, respectively. Anti-N. caninum antibodies were detected in 69 (21.56%; 95% CI: 17.27%-26.56%) dogs, 31 (44.93%) of which lived in urban areas and 38 (55.07%) in rural areas, and endpoint titers ranged from 50 to 3200. Anti-T. gondii antibodies were found in 132 (41.25%; 95% CI: 35.84%-46.87%) dogs, 58 (43.94%) from urban areas and 74 (56.06%) from rural areas, and endpoint titers ranged from 16 to 8192. A total of 33 dogs (10.3%) (12 urban and 21 rural animals) reacted to both agents (P <0.05). Tis study showed a higher prevalence of anti-T. gondii compared to anti-N. caninum antibodies in the dogs living in the Pantanal region, suggesting that both protozoans circulate in the studied region. Tis information is relevant, in view of its implications for animal and public health.

A introdução de novas linhagens de camundongos em biotérios livres de patógenos específicos (SPF) deve ser realizada com critérios para evitar a quebra das barreiras sanitárias. Dessa forma, os animais devem ser rederivados para reduzir os riscos de infecção e evitar as interferências provocadas pela perda do status sanitário e do bem-estar dos animais. O objetivo deste estudo foi implementar a transferência de embriões murinos no Biotério do Departamento de Imunologia do Instituto de Ciências Biomédicas da Universidade de São Paulo, Brasil. As transferências embrionárias foram realizadas para rederivar linhagens de camundongos geneticamente modificadas com status sanitário não conhecido, recebidas de diferentes instituições de pesquisa e de ensino. Os embriões em duas células foram obtidos pelos métodos naturais e transferidos para os ovidutos de fêmeas de camundongos SPF pseudoprenhas. Todos os procedimentos cirúrgicos foramrealizados sob condições assépticas. Um total de 625 embriões foram transferidos para as receptoras. Foram obtidos 148 filhotes nascidos vivos, destes 140 foram desmamados. Por meio desta técnica, foram eliminados vírus, bactérias e protozoários intestinais. A melhora no status microbiológico dos camundongos permitiu a expansão destes em nossa colônia SPF. Com esses resultados, podemos promover o uso da técnica de transferência de embriões entre os biotérios brasileiros e assim incentivar a distribuição de modelos mais adequados para a nossa comunidade científica. | The introduction of new strains of mice in specific pathogen-free (SPF) animal facilities should be performed carefully to avoid breaking sanitary barriers. To meet this need, animals should be rederived to reduce infection risk and thus avoid research interference caused by loss of animal health status and welfare. The objective of this study was to implement mice embryo transfer in the laboratory mouse facility of the Department of Immunology at the Institute of Biomedical Sciences/University of São Paulo, Brazil. Embryo transfers were performed to rederive genetically modified mouse strains with undefined sanitary status, received from different research and educational institutions. Fertilized eggs at two-cell stage were obtained by natural means and transferred into the oviducts of SPF pseudo-pregnant female mice. All surgical procedures were performed under aseptic conditions. A total of 625 embryos were transferred into therecipients. 148 pups were born, of which 140 were reared. Viruses, bacteria and intestinal protozoa were eliminated using this technique. The improvement in the microbiological status of mice allowed their expansion in our SPF facility. With these results, we can stimulate the use of embryo transfer technique between rodent facilities in Brazil and thus encourage the distribution of better models to our scientific community.

The introduction of new strains of mice in specific pathogen-free (SPF) animal facilities should be performed carefully to avoid breaking sanitary barriers. To meet this need, animals should be rederived to reduce infection risk and thus avoid research interference caused by loss of animal health status and welfare. The objective of this study was to implement mice embryo transfer in the laboratory mouse facility of the Department of Immunology at the Institute of Biomedical Sciences/University of São Paulo, Brazil. Embryo transfers were performed to rederive genetically modified mouse strains with undefined sanitary status, received from different research and educational institutions. Fertilized eggs at two-cell stage were obtained by natural means and transferred into the oviducts of SPF pseudo-pregnant female mice. All surgical procedures were performed under aseptic conditions. A total of 625 embryos were transferred into the recipients. 148 pups were born, of which 140 were reared. Viruses, bacteria and intestinal protozoa were eliminated using this technique. The improvement in the microbiological status of mice allowed their expansion in our SPF facility. With these results, we can stimulate the use of embryo transfer technique between rodent facilities in Brazil and thus encourage the distribution of better models to our scientific community.

O Tricoblastoma se caracteriza como uma neoplasia benigna cutânea que tem origem no epitélio tricoblástico do bulbo piloso. O presente relato descreve o caso de felino macho, sem raça definida (SRD), com histórico de nódulo solitário de crescimento lento, não aderido e ulcerado, localizado na região escapular esquerda. A histopatologia evidenciou células neoplásicas organizadas em ninhos coesos, em paliçadas com citoplasma eosinofílico e escasso, os achados foram compatíveis com a descrição de tricoblastoma trabecular. O tratamento de eleição consistiu na exérese cirúrgica total da massa com ampla margem de segurança e recidivas não foram observadas. | Trichoblastoma is characterized as a benign cutaneous neoplasm that originates in the trichoblastic epithelium of the hair bulb. The present report describes the case of a 4-year-old cross breed neutered male cat that had a history of a slow, freely movable, ulcerated, slow growing, solitary cutaneous nodule located on the left scapular region. Histopathological evaluation showed neoplastic cells arranged in cohesive nests or palisades with eosinophilic and scant cytoplasm. Based on the microscopic findings, a diagnosis of trabecular trichoblastoma was made. The treatment of choice consisted of total surgical excision of the mass with safe surgical margins. Tumor recurrence was not observed.

Trichoblastoma is characterized as a benign cutaneous neoplasm that originates in the trichoblastic epithelium of the hair bulb. The present report describes the case of a 4-year-old cross breed neutered male cat that had a history of a slow, freely movable, ulcerated, slow growing, solitary cutaneous nodule located on the left scapular region. Histopathological evaluation showed neoplastic cells arranged in cohesive nests or palisades with eosinophilic and scant cytoplasm. Based on the microscopic findings, a diagnosis of trabecular trichoblastoma was made. The treatment of choice consisted of total surgical excision of the mass with safe surgical margins. Tumor recurrence was not observed.

A rangeliose canina é uma doença hemolítica extravascular causada pelo protozoário Rangelia vitalii, o qual é transmitido por carrapatos da espécie Amblyomma aureolatum. Os sinais clínicos mais comuns são apatia, hipertermia e sangramentos espontâneos. Os achados hematológicos mais comuns são anemia e trombocitopenia. Este trabalho teve como objetivo relatar um caso clínico de Rangeliose canina tratada em um hospital veterinário particular, na cidade de São Paulo no ano de 2017. Um cão foi atendido em um hospital veterinário da zona norte de São Paulo, com emagrecimento progressivo, apatia e lesão na cauda. No hemograma foram observadas anemia e trombocitopenia. Através da PCR em tempo real (qPCR) do sangue do animal constatou-se a presença de DNA de Rangelia vitalii. Além do tratamento de suporte, utilizou-se doxiciclina e aplicações subcutâneas de imidocarb. A amostra coletada após o tratamento com o antibiótico continuou apresentando DNA do protozoário. A enfermidade deve ser considerada como diagnóstico diferencial e há uma grande necessidade de maiores estudos acerca da terapia utilizada. | Canine rangeliosis is an extravascular hemolytic disease caused by the protozoan Rangelia vitalii, which is transmitted by ticks of the species Amblyomma aureolatum. Te most common clinical signs are apathy, hyperthermia and spontaneous bleeding. Anemia and thrombocytopenia are the most common hematological fndings. Tis work reports a clinical case of canine Rangeliosis treated at a private veterinary hospital, in São Paulo city in 2017. A dog was treated at a veterinary hospital in the north of São Paulo, with progressive weight loss, apathy and tail injury. Anemia and thrombocytopenia were observed on the hemogram. Rangelia vitalii DNA was detected in animal blood by real-time PCR (qPCR). In&nbsp;addition to the supportive treatment, doxycycline and subcutaneous imidocarb applications were used. Te sample collected afer treatment with the antibiotic continued to present protozoal DNA. Te disease should be considered as a diferential diagnosis and there is a great need for further studies about the therapy used.

Canine rangeliosis is an extravascular hemolytic disease caused by the protozoan Rangelia vitalii, which is transmitted by ticks of the species Amblyomma aureolatum. Te most common clinical signs are apathy, hyperthermia and spontaneous bleeding. Anemia and thrombocytopenia are the most common hematological fndings. Tis work reports a clinical case of canine Rangeliosis treated at a private veterinary hospital, in São Paulo city in 2017. A dog was treated at a veterinary hospital in the north of São Paulo, with progressive weight loss, apathy and tail injury. Anemia and thrombocytopenia were observed on the hemogram. Rangelia vitalii DNA was detected in animal blood by real-time PCR (qPCR). In addition to the supportive treatment, doxycycline and subcutaneous imidocarb applications were used. Te sample collected afer treatment with the antibiotic continued to present protozoal DNA. Te disease should be considered as a diferential diagnosis and there is a great need for further studies about the therapy used.

Peixes ornamentais de aquário representam um grande segmento no mercado de animais de estimação, no qual Estados Unidos, Europa e Japão dominam. São aproximadamente 1.500 espécies de peixes marinhos e em torno de 4.500 de espécies de água doce comercializados com fins ornamentais. O peixe é a quarta espécie mais comum nos domicílios brasileiros. Peixes ornamentais de aquário são comercializados e distribuídos em diferentes partes do território nacional e do mundo. O comércio e circulação de animais vivos sem o uso de procedimentos de manejo profilático adequados possibilita a disseminação de inúmeros agentes patogênicos. Peixes ornamentais de aquário carreiam consigo agentes patogênicos de etiologia bacteriana, viral, fúngica e parasitária, sendo alguns de caráter zoonótico colocando em riscopessoas que os manipulam. O objetivo desta revisão é apresentar os principais agentes infeciosos patogênicos - de natureza bacteriana, viral e fúngica - que acometem peixes ornamentais de aquário, bem como os métodos de prevenção e controle que permitam excelência no segmento. | Aquarium ornamental pet fish constitute a major segment in the pet industry, with the United States, Europe, and Japan dominating the market. There are approximately 1,500 marine fish species and over 4,500 freshwater fish species commercialized as aquarium ornamental pet fish. Fish are the fourth most common pet present in Brazilian homes. In Brazil, aquarium ornamental pet fish can be marketed and distributed from different parts of the Brazilian territory and the world. Commercialization and circulation of living animals without the use of adequate prophylactic management procedures enables dissemination of a number of agents responsible for infectious diseases. Aquarium pet fish can also carry pathogenic agents, of bacterial, viral, fungal, or parasitic etiology, that may have a zoonotic feature endangering the persons handling the animals. This review presents the main pathogenic infectious agents of bacterial, viral, andfungal etiology that affect aquarium pet fish, as well as the prevention and control measures to ensure sanitary excellence in this segment.

Aquarium ornamental pet fish constitute a major segment in the pet industry, with the United States, Europe, and Japan dominating the market. There are approximately 1,500 marine fish species and over 4,500 freshwater fish species commercialized as aquarium ornamental pet fish. Fish are the fourth most common pet present in Brazilian homes. In Brazil, aquarium ornamental pet fish can be marketed and distributed from different parts of the Brazilian territory and the world. Commercialization and circulation of living animals without the use of adequate prophylactic management procedures enables dissemination of a number of agents responsible for infectious diseases. Aquarium pet fish can also carry pathogenic agents, of bacterial, viral, fungal, or parasitic etiology, that may have a zoonotic feature endangering the persons handling the animals. This review presents the main pathogenic infectious agents of bacterial, viral, and fungal etiology that affect aquarium pet fish, as well as the prevention and control measures to ensure sanitary excellence in this segment.

O frênulo peniano é uma fina faixa de tecido conectivo que une a glânde ao prepúcio que pode se romper sob a ação da testosterona quando o animal inicia a puberdade. Devido a escassez de relatos desta afecção o presente trabalho relata a ocorrência de frênulo peniano persistente em um gato doméstico, sem raça definida, de sete meses de idade e 5,2 kg. O frênulo peniano foi um achado incidental durante o exame clínico do animal. O animal já apresentava‑se em puberdade, caracterizando o quadro de frênulo peniano. A resolução do caso ocorreu de maneira espontânea previamente a orquiectomia eletiva. Dessa forma, as alterações observadas no pênis devido a alteração congênita em gatos são descritas neste relato. &nbsp; | The penile frenulum is a thin strip of connective tissue that connects the penis glans to the foreskin. Due to the few number of reports of this condition, this work reports the occurrence of persistent penile frenulum in a mixed breed domestic cat, of seven months old and 5.2 kg. In this particular case, penile frenulum was an incidental finding during clinical examination. The animal was in puberty, characterizing the penile frenulum. The resolution of the case occurred spontaneously prior to elective orchiectomy. Thus, the changes observed in the penis due to congenital alteration in cats are described in this report. &nbsp;

O frênulo peniano é uma fina faixa de tecido conectivo que une a glânde ao prepúcio que pode se romper sob a ação da testosterona quando o animal inicia a puberdade. Devido a escassez de relatos desta afecção o presente trabalho relata a ocorrência de frênulo peniano persistente em um gato doméstico, sem raça definida, de sete meses de idade e 5,2 kg. O frênulo peniano foi um achado incidental durante o exame clínico do animal. O animal já apresentava‑se em puberdade, caracterizando o quadro de frênulo peniano. A resolução do caso ocorreu de maneira espontânea previamente a orquiectomia eletiva. Dessa forma, as alterações observadas no pênis devido a alteração congênita em gatos são descritas neste relato.

O presente trabalho descreve os sinais clínicos, as lesões macroscópicas e os aspectos histológicos observados em 25&nbsp;cordeiros acometidos em um surto de artrogripose congênita com hidrocéfalo ou hidrocefalia registrado no Iraque, governadoria Al-Muthama após a ocorrência de nascimentos prematuros e deformidades músculo-esqueléticas no período compreendido entre outubro de 2017 e maio de 2018. A hipoplasia músculo-esquelética foi observada nos membros dos cordeiros afetados, acompanhada de severa artrogripose e malformações cerebrais, grosseiras visíveis, além de mumifcações fetais, nascimentos prematuros e morte de cordeiros. Os principais aspectos histopatológicos nas fbras musculares foram lesões degenerativas e ausências da estriação cruzada acompanhada de leve infltração de neutróflos e células mononucleares dos cordeiros severamente afetados. As meninges dos cordeiros afetados apresentaram fusão de membranas com áreas focais de espessamento fbroso e debris necróticos. O vírus Akabane, um membro do gênero Orthobunyavirus, da família Bunyaviridae, causa anormalidades congênitas e artrogripose com hidrocéfalo e hidrocefalia em ruminantes e poderá vir a ser a causa do presente surto. Os autores recomendam a realização de novos estudos com investigações epidemiológicas e isolamento do agente causal. Contudo, outras causas de hidrocéfalo ou malformações cerebrais como as determinadas pelo vírus Schmallenberg, vírus da língua azul e vírus da doença de border, bem como de plantas teratogênicas que determinam a artrogripose, também deverão ser investigadas. As autoridades sanitárias deverão tomar medidas de prevenção e controle para bloquear a replicação do vírus em artrópodes vetores. | Akabane virus, a member of the Orthobunyavirus genus in the family Bunyaviridae, causes congenital abnormalities and arthrogryposis with hydrocephalus or hydroencephaly in ruminants. Tis study intends to describe the clinical signs, gross and histopathological features seen in 25 afected lambs in an outbreak of congenital arthrogryposis with hydrocephalus or hydranencephaly in Al-Muthanna governorate, Iraq afer a large number of stillbirths and musculoskeletal deformities from October 2017 to May 2018. Skeletal muscle hypoplasia was seen in the limbs of the afected lambs accompanied with severe arthrogryposis and gross visible brain malformations. In addition, fetal mummifcations, stillbirths, and dead lambs were also seen. Te most histopathological features in muscle fbers were degenerative lesions and absences of cross-striation accompanied with mild infltration of neutrophils and mononuclear cells in severely afected lambs. Te meninges of afected lambs revealed fused membranes with focal areas of fbrous thickenings and necrotic debris. In conclusion, according to clinical signs, gross and histopathological investigations, Akabane virus, a member of the Orthobunyavirus genus in the family Bunyaviridae, causes congenital abnormalities and arthrogryposis with hydrocephalus or hydroencephaly in ruminants and could be the cause of this outbreak, although future studies must be performed to confrm the etiology of this outbreak. Moreover, other causes of hydrocephalus or cerebellar malformation, such as Schmallenberg virus, bluetongue virus and border disease virus and teratogenic plants that lead to arthrogryposis, have to be investigated. Also, the authorities should take prevention and control measurements to stop the replication of arthropod vectors.

Akabane virus, a member of the Orthobunyavirus genus in the family Bunyaviridae, causes congenital abnormalities and arthrogryposis with hydrocephalus or hydroencephaly in ruminants. Tis study intends to describe the clinical signs, gross and histopathological features seen in 25 afected lambs in an outbreak of congenital arthrogryposis with hydrocephalus or hydranencephaly in Al-Muthanna governorate, Iraq afer a large number of stillbirths and musculoskeletal deformities from October 2017 to May 2018. Skeletal muscle hypoplasia was seen in the limbs of the afected lambs accompanied with severe arthrogryposis and gross visible brain malformations. In addition, fetal mummifcations, stillbirths, and dead lambs were also seen. Te most histopathological features in muscle fbers were degenerative lesions and absences of cross-striation accompanied with mild infltration of neutrophils and mononuclear cells in severely afected lambs. Te meninges of afected lambs revealed fused membranes with focal areas of fbrous thickenings and necrotic debris. In conclusion, according to clinical signs, gross and histopathological investigations, Akabane virus, a member of the Orthobunyavirus genus in the family Bunyaviridae, causes congenital abnormalities and arthrogryposis with hydrocephalus or hydroencephaly in ruminants and could be the cause of this outbreak, although future studies must be performed to confrm the etiology of this outbreak. Moreover, other causes of hydrocephalus or cerebellar malformation, such as Schmallenberg virus, bluetongue virus and border disease virus and teratogenic plants that lead to arthrogryposis, have to be investigated. Also, the authorities should take prevention and control measurements to stop the replication of arthropod vectors.