BACKGROUND: Ovine theileriosis is an important hemoprotozoal disease of sheep and goats in tropical and subtropical regions which causes high economic loss in the livestock industry. OBJECTIVES: The aim of this study was the differential detection of Theileria species in sheep using PCR method. METHODS: Two hundred blood samples of sheep were investigated in order to differentially diagnose Theileria species. DNA was extracted from blood samples and DNA samples were amplified using specific primers designed for 18S rRNA, TamS1 and TaSp genes. RESULTS: In this study, from 200 examined samples, 42 samples (21%) were infected by Theileria spp. and none of them were infected by Babesia spp. Moreover, from these 42 positive samples, 24 samples (57.1%) were only infected by T. ovis. 12 samples (28.5%) were only infected by T. lestoquardi, 2 samples (4.7%) were only infected by T. annulata and 4 samples (9.5%) were simultaneously infected by T. lestoquardi and T. ovis. The results of nucleotide sequencing showed that PCR product of 18S rRNA from T. lestoquardi has 99 and 95% similarity with T. annulata and T. ovis respectively. T. lestoquardi and T. annulata showed 86% similarity. Also TaSp gene of T. ovis in comparison with T. annulata and T. lestoquardi showed 96 and 86% similarity, respectively. CONCLUSIONS: In the present study could be shown that the two genes (TamS1 and TaSp) from examined three genes could be used for Theileria species specific diagnosis by PCR.

BACKGROUND: Application of different source of minerals and their effect on metabolic performance a current issue in animal science and research centers. OBJECTIVES: The objective of this study was synthesis of selenium nanoparticles and to investigate its effect compared with other selenium sources on the blood parameters associated with the liver functional index of Holstein dairy cow. METHODS: This study was conducted in two phases: laboratory and farm. Oxidation-reduction chemical reactions were used to synthesize selenium nanoparticles. The formation and non-change of Se nanoparticles size were confirmed by particle size analyzer. In order to perform farm experiment, 6 dairy cows were allocated into three treatments and 2 replicates in a 3×3 Latin square experiment with 28 days change over the period. The animals were distributed to three treatment groups. Treatments (diets) were 1) control (sodium selenite source), 2) colloid sodium selenite and 3) selenium nanoparticles. Blood parameters associated with the liver functional index, including albumin, bilirubin and total cholesterol were measured in the present experiment. Also, dry matter intake of cows was recorded. RESULTS: The blood plasma cholesterol of cows fed the colloidal sodium selenite was higher than cows fed the selenium nanoparticles (P-Value =0.01). The blood serum albumin (P-Value =0.03) and bilirubin levels (P-Value =0.04) were lower in group 3 compared with group 1. Dry matter intake was not affected by treatments. CONCLUSIONS: According to the results of this experiment it seems selenium nanoparticles supplementation   improves blood parameters associated with the liver functional index, which can better improve immune system of dairy cows than two other sources of selenium.

BACKGROUND: Staphylococcus aureus is one of the most important human pathogens that cause infection and also food intoxication by secreting various enterotoxins. Conventional culturing methods to detect S. aureus have some limitations such as being time-consuming due to bacterial growth and low precision and sensitivity in detecting viable but non-cultivable cells. OBJECTIVES: The objective of this study was to detect and quantify enterotoxigenic (A-E) S. aureus in cream pastry products applying PCR coupling with propidium monoazide (PMA) to distinguish dead and live cells. METHODS: One hundred samples were randomly collected from pastry shops in Amol, in a period of 2 months. After preparing dilutions, bacterial pellets were separated and treated with PMA before DNA extraction. Real time PCR was conducted in order to quantify S.aureus cells and enterotoxigenic strains using specific primers. RESULTS: Results of conventional method were close to PMA-qPCR data (P>0.05), but data from qPCR that includes live and dead cells shows more bacterial count than two other methods. Sensitivity of the method applied in the present study, detecting low number of S.aureus cells (less than 10/g) seems considerable. CONCLUSIONS: Findings showed that applying PCR coupling with PMA results in more reliable data than conventional culturing method. Regarding this approach being time-effective, considerably sensitive and specific, it is expected that it be used in food quality control labs in monitoring systems in future.

BACKGROUND: Titanium dioxide nanoparticles (TiO2NPs) are used in many commercial products, including paints, sunscreens and edible products, and can be used directly and indirectly in aquaculture. Due to the widespread application of these nanoparticles, the investigation of their possible effects on aquatic organisms is necessary. OBJECTIVES: The purpose of this study was to investigate the histopathological effects of waterborne TiO2NPs on the gills, liver and intestine of Caspian trout. METHODS: In this study, 126 fish (27.46±4.3 g) were tested in six concentrations of TiO2NPs (0, 0.01, 0.1, 1, 10 and 100 mg/L) for 4 days. At the end of the experiment, tissues were evaluated to determine the effects of tissue damage caused by TiO2NPs. RESULTS: The 96-h exposure to TiO2NPs did not cause fish mortality. Accordingly, investigated TiO2NPs are classified as "relatively non-toxic". The most important tissue changes caused by short-term exposure to TiO2NPs were hyperplasia and hypertrophy, lamellar fusion, necrosis in gills; foci of melanomacrophage deposits, aggregation of blood cell, hepatocytes with pycnotic nuclei, vacuolation, necrosis in liver; thickening of lamina propria, erosion of villi and necrosis in intestine. CONCLUSIONS: Although TiO2NPs are relatively non-toxic,  due to the tissue damage observed, these nanoparticles can be harmful to Caspian trout.

BACKGROUND: Diarrhea syndrome is associated with irrecoverable damages in the husbandry industry worldwide due to losses resulted from fatality, weight loss, growing weak calves and treatment costs. Hence, investigation of diarrhea causes in different areas is important to attempt management strategies to prevent and control it. OBJECTIVES: Present study was carried to investigate prevalence of some important entropathogens in diarrheic calves until three months old, in Shahrekord suburb husbandries. METHODS: Fecal samples were taken from 82 female calves in first day of diarrhea and were examined for isolation of salmonella, Escherichia coli, clostridium, cryptosporidium, and coccidia through common microbiological and parasitological methods. RESULTS: In general, prevalence of isolated organisms were: salmonella 36.6%, Escherichia coli 24.4%, clostridium 9.8%, cryptosporidium 9.8%, and coccidian 7.31%, and Escherichia coli K99 were isolated from four calves. The most prevalent pathogens were Escherichia coli and Salmonella. CONCLUSIONS: The calves are unavoidably exposed to infectious causes of diarrhea during their whole lifespan, because they acquire organisms from environment immediately after birth. Therefore, attempts at efficient management methods, hygienic principles and receiving enough colostrum, particularly in cold seasons, may be efficient in the control, prevention and decrease of diarrhea and its subsequent losses.

BACKGROUND: Heavy metals and metalloids are among the most stable and resistant to decomposition elements that, in the presence of high concentrations in aquatic tissues, threaten their own and human health. OBJECTIVES: The aim of this study was to determine the composition and order of the elements accumulation in the edible muscle tissue of pike from Siah Darvishan River as one of the most valuable and economical fish and also to evaluate its health for human nutrition. METHODS: In the present study, concentration of eleven elements (As, Fe, Hg, Zn, Pb, Se, Cd, Cr, Cu, Mn, Ni) in the muscle tissue of 25 pike (Esox lucius) collected with the Cast net in the summer of 2016 from Siah Darvishan River, Guilan province, Iran were surveyed by Varian Atomic absorption spectroscopy. RESULTS: The lowest and highest mean accumulation of elements were obtained as follows: Zinc 25.89 - 28.80, Iron 21.85 - 24.26, Copper 6.78 - 8.15, Manganese 1.77 - 2.12, Lead 0.68 - 0.73, Selenium 0.59 - 0.62, Arsenic 0.53 - 0.62, Cadmium 0.38 - 0.41, Nickel 0.24 - 0.28, Chromium 0.17 - 0.19 and Mercury 0.087 - 0.098 microgram per gram dry weight, respectively. CONCLUSIONS: According to the results of this study, mean concentrations of all elements in the muscle tissue of pike, except the four elements Manganese (1.956 ± 0.178), Lead (0.698 ± 0.028), Arsenic (0.572 ± 0.044) and Cadmium (0.393 ± 0.018) were observed to be lower than explained threshold limit value (TLV) of FAO/WHO international standard.

BACKGROUND: Nowadays due to the extensive use of antibiotics, antimicrobial resistance is recognized as a global concern for the health of animals and humans. The consequences of antimicrobial resistance are resistance of zoonotic pathogenic factors and reductions in the effectiveness of treatment for many diseases. OBJECTIVES: The aims of this study are the monitoring and prevalence of antimicrobial residues of the poultry carcasses in Kerman poultry abattoirs. METHODS: A cross sectional study was conducted from June 2015 to October 2016. A total of 474 samples were collected from Kerman province poultry abattoirs and tested with four plate test (FPT) method. Data were analyzed  using Descriptive statistics for qualitative data with 95 % confidence interval. RESULTS: A total of 38 samples were positive (8/02 %), Antibiotics isolates included: Fluoroquinolone, Macrolide, Cephalosporin, β-lactam, Tetracycline, Sulfonamide and Amino glycoside. The highest rate of antibiotics isolated in positive samples was Fluoroquinolone (36/1 %) and the lowest rate was cephalosporin (4/1 %). Half of the samples were positive for one type of antibiotic (Fluoroquinolone), 28/9 % for two types of antibiotics, 13/1 % for three types of antibiotics, 5/2%  for five kinds of antibiotics and 2/6 % for six kinds of antibiotics. CONCLUSIONS: The results of the study showed that about 10 percent of the meat consumed in this province has antimicrobial residues. Therefore, it can be suggested that proper management strategies for controlling the veterinary drug usage in poultry farms be implemented.

BACKGROUND: Abomasal hypomotility plays an important role in pathogenesis of some abomasal disorders such as abomasal bloat which has the same serious side effects associated with using synthetic drugs for its treatment, such as diarrhea and antibiotic resistance. To decreasing these side effects, administration of herbal medicine is a good way. OBJECTIVES: To determine the effect of oral administration of turmeric aqueous extract on rate of abomasal emptying rate in neonatal lambs. METHODS: This study was conducted on twelve five-day-old Sangsari-female-lambs (average weight 3 kg). All lambs received five oral treatments, including saline (30 ml), erythromycin (400 mg), turmeric 200 mg/kg, turmeric 250 mg/kg, and turmeric 300 mg/kg, respectively. At 0, 30, 60, 90, 120, 150, 180, and 240 minutes after each treatment, plasma samples of lambs were taken. The rate of abomasal emptying was determined with acetaminophen absorption test. RESULTS: Treatment with erythromycin and three different doses of aqueous extract of turmeric (200, 250, 300 mg/kg) increased the rate of abomasal emptying in comparison to the negative control treatment, significantly (P<0.05). The stimulatory effect of erythromycin on abomasal emptying was higher than aquatic extract of turmeric, significantly (P<0.05). No clinical side effects were observed following the administration of erythromycin and turmeric in lambs. CONCLUSIONS: This study showed that aqueous extract of turmeric has a stimulatory effect on lamb's abomasal emptying but more studies are needed on the effect of this plant’s components on abomasal emptying.

BACKGROUND: Many of the negative effects of heavy metals can be reduced by diets containing anti-oxidant compounds such as vitamins and herbs. OBJECTIVES: The aim of this study is evaluation of the effect of Clove essential oil and Ascorbic acid on growth performance and intestinal villi morphology in Japanese quail following oral administration of Lead. METHODS: A total of 120 one-day old quails were divided into 4 groups with 30 quails in each group (3 replicates). The group 1 was fed with basal diet, the groups 2 and 3 were fed with basal diet + 150 and 450 mg/kg diet clove essential oil respectively, the group 4 was fed with basal diet + 500 ppm ascorbic acid (vitamin C) in diet. All groups received 100 ppm lead acetate in drinking water during the experimental period (7-35 days). At day 35, 3 segments of intestine (duodenum, jejunum and ileum) were isolated. RESULTS: The group clove 450 ppm and ascorbic acid had significantly more body weight than control (P<0.05). Also, the group clove 150, 450 ppm and ascorbic acid had significantly less Feed Conversion Rate (FCR) than control (P<0.05). The group clove 450 ppm and ascorbic acid had significantly greater villi dimensions and absorptive surface in the different segments of the intestine in comparison to control (P<0.05). CONCLUSIONS: The results showed that adding ascorbic acid and clove essential oil to Japanese quail diet, reduced the negative effects of lead on body weight, feed conversion ratio and intestinal villi absorptive surface.

BACKGROUND: This study was conducted to characterize two fowlpox viruses isolated in Iran and study their phylogenic relationship. OBJECTIVES: The aim of this study was to determine the identity of two pox viruses recovered from a tissue sample with typical lesions of fowl pox and red mites from a laying farm. Fowl pox in poultry industry causes significant economic losses associated with decreased egg production, reduced growth, blindness and increased mortality. The pox virus, by direct or indirect contact, may also be transmitted through vectors such as mites and fleas. Dermanyssus gallinae also known as the poultry red mite is the most important blood feeder ectoparasite affecting poultry, in particular, laying hens. In addition to its significant economic losses, it is considered as a potential vector for a number of important avian pathogens. METHODS: Both isolates were inoculated to chorioallantoic membrane (CAM) of embryonated chicken eggs, detected by specific PCR for pox virus 4b gene and characterized by sequencing and phylogentic analysis. RESULTS: Both isolates developed pock lesions in CAM of embryonated eggs. A 578 bp fragment of the poxvirus 4b gene was amplified in both isolates by PCR. The sequence analyses revealed that the two isolates were 100% identical and placed in the same branch. The nucleotide sequence of these two isolates showed a similarity of 73 to 100% to the other selected avian pox sequences available in the GenBank. CONCLUSIONS: These results confirmed that Dermanyssus gallinae are considered to be an important vector for the spread of fowl pox virus in the flocks. Therefore, controlling the red mites in poultry farms will not only inhibit problems such as blood feeding, decreased production and reduced growth rate, it can reduce the transmission of diseases and pathogenic agents in the farms.