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Cobre nanoestruturado como fonte alternativa ao sulfato de cobre como melhorador de desempenho para leitões em fase de creche | Nanostructured copper as an alternative source to copper sulphateas a growth promoter for nursery piglets Texte intégral
2025
Santos, Rafaela Nunes dos | Alves, Laya Kannan Silva | Coelho, Flávio de Aguiar | Silveira, Hebert | Lange, Camila Neves | Gonçalves, Joaquim Carlos Atra | Tremiliosi, Guilherme Carvalho | Schinckel, Allan Paul | Garbossa, Cesar Augusto Pospissil | Santos, Rafaela Nunes dos | Alves, Laya Kannan Silva | Coelho, Flávio de Aguiar | Silveira, Hebert | Lange, Camila Neves | Gonçalves, Joaquim Carlos Atra | Tremiliosi, Guilherme Carvalho | Schinckel, Allan Paul | Garbossa, Cesar Augusto Pospissil
Antimicrobianos são comumente utilizados na produção suinícola para melhorar o desempenho dos animais, no entanto, seu uso pode contribuir para o desenvolvimento de resistência bacteriana. Como resultado, há a necessidade de explorar novas tecnologias nutricionais que possam promover a saúde e a produtividade animal sem comprometer a resistência bacteriana. Este estudo teve como objetivo avaliar os efeitos do cobre nanoestruturado como melhorador de desempenho para leitões na fase de creche. Um total de 135 leitões com 28 dias de idade foram distribuídos em um delineamento em blocos casualizados (sexo e peso inicial) e divididos em cinco tratamentos dietéticos: controle negativo (sem melhorador de desempenho); controle positivo (inclusão de 200 mg/kg de sulfato de cobre); e três dietas com diferentes inclusões de cobre nanoestruturado (10, 20 e 30 mg/kg da dieta). Os parâmetros de ganho de peso diário (GPD), consumo de ração diário (CRD) e conversão alimentar (CA) foram usados para caracterizar o desempenho dos leitões na fase de creche. Escores fecais diários foram usados para caracterizar a prevalência de distúrbios entéricos e a análise econômica foi usada como um parâmetro para estimar a viabilidade econômica da suplementação de cobre nanoestruturado para leitões desmamados. Aos sete dias do experimento foi observado que os animais do grupo controle negativo apresentaram peso vivo superior (Plt;0,05) ao grupo com sulfato de cobre em 7,75%. Do dia 21 ao 42 do experimento verificou-se que os animais do grupo com sulfato de cobre apresentaram CRD superior (Plt;0,05) em comparação a todos os outros grupos experimentais. Para o escore fecal médio, o grupo com inclusão de 20 mg/kg de cobre nanoestruturado apresentou escore superior (Plt;0,05) em 13% em comparação ao grupo do sulfato de cobre, demonstrando maior incidência de diarreia. Portanto, o cobre nanoestruturado, nas doses avaliadas, não foi eficaz em melhorar o desempenho de leitões na fase de creche. | Antimicrobial feed additives are commonly used in swine production to improve pig health and performance, but their use can contribute to the development of bacterial resistance. In this sense, new nutritional technologies that promote animal health and productivity without compromising bacterial susceptibility must be evaluated. This study aimed to assess the effects of nanostructured copper as a growth promoter for piglets in the nursery phase. A total of 135 piglets at 28 days of age were distributed in a randomized block design (sex and initial weight) and assigned to five dietary treatments: negative control (without growth promoters); positive control (200 mg/kg copper sulfate inclusion); and three diets with different nanostructured copper inclusions (10, 20, and 30 mg/kg of the diet). Body weight (BW), average daily weight gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were used to characterize piglet performance in the nursery phase. Daily fecal scores were used to describe the prevalence of enteric disorders, and economic analysis was used as a parameter of the financial viability of nanostructured copper supplementation for weaned piglets. After seven days of the experiment, the negative control group pigs had 7.75% greater BW (Plt;0.05) than the copper sulfate group pigs. From day 21 to 42 of the experiment, the copper sulfate group animals had a greater ADFI (Plt;0.05) than all other experimental groups. For the average fecal score, pigs fed the 20 mg/kg nanostructured copper inclusion had a 13 percent higher score (Plt;0.05) than the copper sulfate group, indicating a greater incidence of diarrhea. Therefore, at the evaluated dietary concentrations, nanostructured copper was ineffective in improving piglet performance in the nursery phase.
Afficher plus [+] Moins [-]Urease Test in Horses: associations between positive test results and bacteria other than Helicobacter sp. | Teste de Urease em Equinos: associações entre resultados de testes positivos e outras bactérias além de Helicobacter sp. Texte intégral
2025
Piffer, Maria Letícia Tescaro | Silva, Luis Claudio Lopes Correia da | De Zoppa, André Luís do Valle | Benites, Nilson Roberti | Melville, Priscilla Anne | Delboni, Cássia Cestari | Barros, Aline de Matos Curvelo de | Belli, Carla Bargi | Piffer, Maria Letícia Tescaro | Silva, Luis Claudio Lopes Correia da | De Zoppa, André Luís do Valle | Benites, Nilson Roberti | Melville, Priscilla Anne | Delboni, Cássia Cestari | Barros, Aline de Matos Curvelo de | Belli, Carla Bargi
This study was designed to examine positivity rates of the urease test (UT) in gastric samples obtained from adult horses and to determine whether positive test results are associated with the presence of bacteria other than Helicobacter sp. Gastric content samples and biopsy specimens from the glandular and non glandular regions of the stomach were collected from 39 adult horses via gastroscopy. Samples were placed in tubes containing urease test medium and incubated in a water bath at 37 C for 24 h. Urease test-positive samples were submitted to bacterial culture and biochemical tests for potential isolation and identification of urease-producing bacteria. Positive UT results were obtained in eight horses. Laboratory analysis revealed the presence of Providencia sp., Escherichia coli, Pseudomonas aeruginosa, Citrobacter amalonaticus, Edwardsiella tarda, Klebsiella oxytoca, Proteus mirabilis, and Klebsiella pneumoniae. In conclusion, changes in urease test medium color after more than 12 h of incubation suggest the presence of bacteria other than Helicobacter sp. However, relationships between positive UT results, types of bacteria detected, and their role in the etiopathogenesis of gastric ulcers in horses remain to be determined. | Este estudo foi delineado para avaliar as taxas de positividade do teste da urease (TU) em amostras gástricas obtidas de cavalos adultos e determinar se os resultados positivos dos testes estão associados à presença de outras bactérias além de Helicobacter sp. Amostras de conteúdo gástrico e amostras de biópsia da região glandular e não glandular do estômago foram coletadas de 39 cavalos adultos por meio de gastroscopia. As amostras foram colocadas em tubos contendo meio teste de urease e incubadas em banho-maria a 37oC por 24 horas. Amostras positivas para teste de urease foram submetidas a cultura bacteriana e testes bioquímicos para potencial isolamento e identificação de bactérias produtoras de urease. Resultados positivos do TU foram obtidos em oito cavalos. A análise laboratorial revelou a presença de Providencia sp., Escherichia coli, Pseudomonas aeruginosa, Citrobacter amalonaticus, Edwardsiella tarda, Klebsiella oxytoca, Proteus mirabilis e Klebsiella pneumoniae. Conclui-se que alterações na cor do meio de teste de urease após mais de 12 horas de incubação sugerem a presença de outras bactérias além de Helicobacter sp. Porém, as relações entre resultados positivos de TU, tipos de bactérias detectadas e seu papel na etiopatogenia das úlceras gástricas em cavalos ainda não foram determinadas.
Afficher plus [+] Moins [-]Wildlife as important hosts of ticks in the flooded savanna of Colombia | Animais selvagens como importantes hospedeiros de carrapatos na savana inundada da Colômbia Texte intégral
2025
Rodríguez-Durán, Arlex | Robayo-Sánchez, Laura Natalia | Bentez-Molano, Jannet | Chaparro-Gutiérrez, Jenny Jovana | Cortés-Vecino, Jesús Alfredo | Rodríguez-Durán, Arlex | Robayo-Sánchez, Laura Natalia | Bentez-Molano, Jannet | Chaparro-Gutiérrez, Jenny Jovana | Cortés-Vecino, Jesús Alfredo
Existem poucos registros de animais selvagens servindo como hospedeiros de carrapatos na região de savana inundada da Orinoquia, na Colômbia; portanto, o objetivo deste estudo foi identificar e mapear os carrapatos que infestam esses animais. Foram amostrados dez locais do município de Arauca, departamento de Arauca, onde foram coletados carrapatos que infestavam 19 animais selvagens, classificados em 14 espécies, fornecendo novas informações sobre infestação por carrapatos nos táxons (i) aves: Crax daubentoni, (ii) mamíferos: Dasypus sabanicola, Dicotyles tajacu, Hydrochoerus hydrochaeris, Leopardus tigrinus, Myrmecophaga tridactyla, Odocoileus virginianus, Procyon cancrivorus, Urocyon cinereoargenteus e Tamandua tetradactyla e (iii) répteis: Boa constrictor, Chelonoidis denticulata, Eunectes murinus e Iguana iguana. Foram identificados 85 carrapatos, classificados nas espécies Amblyomma mixtum, com 72,8%, seguido por Amblyomma dissimile, com 11,7%, Rhipicephalus microplus, com 8,2%, Dermacentor nitens, com 4,7% e Amblyomma nodosum, com 2,3%. Nos carrapatos, a espécie Amblyomma mixtum foi aquele em que ocorreu maior variedade de infestações aos hospedeiros, parasitando 53,8%. Hydrochoerus hydrochaeris foi o que apresentou maior variedade de espécies de carrapatos, sendo infestado por Amblyomma mixtum e Dermacentor nitens. A diversidade e a ampla distribuição destes animais selvagens tornam-nos potenciais hospedeiros de carraças de importância médica e veterinária, tendo em conta a estreita interação ambiental, cultural e econômica que se desenvolveu entre animais selvagens, domésticos, carraças, agentes patogênicos e humanos. | Records of wild animals acting as tick hosts in the flooded savanna region of Colombia’s Orinoquia are scarce. Therefore, this study aimed to identify and map the ticks that infest these hosts. Ten locations were sampled in the municipality of Arauca, department of Arauca, Colombia, where ticks were collected infesting 19 wild animals, classified into 14 species, providing new information on tick infestation in the following taxa: (i) bird: Crax daubentoni, (ii) mammals: Dasypus sabanicola, Dicotyles tajacu, Hydrochoerus hydrochaeris, Leopardus tigrinus, Myrmecophaga tridactyla, Odocoileus virginianus, Procyon cancrivorus, Urocyon cinereoargenteus and Tamandua tetradactyla, and (iii) reptiles: Boa constrictor, Chelonoidis denticulata, Eunectes murinus and Iguana iguana. A total of 85 ticks were identified and classified as the species Amblyomma mixtum, corresponding to 72.8%, followed by Amblyomma dissimile – 11.7%, Rhipicephalus microplus – 8.2%, Dermacentor nitens – 4.7%, and Amblyomma nodosum – 2.3%. Amblyomma mixtum was the tick species that showed the highest variety of infestations to hosts, parasitizing 53.8%. The host infested with the greatest variety of tick species was Hydrochoerus hydrochaeris, from which Amblyomma mixtum and Dermacentor nitens were removed. The diversity and wide distribution of these wild animals make them potential tick hosts of medical and veterinary importance, given the close environmental, cultural, and economic interaction that has developed between wild and domestic animals, ticks, pathogens, and humans.
Afficher plus [+] Moins [-]High frequency of the occurrence of Giardia spp. in dogs in veterinary care in a scrubland area in Goiás state, Brazil | Alta frequência de ocorrência de Giardia spp. em cães em uma área do cerrado no estado de Goiás, Brasil Texte intégral
2025
Gomes, Ana Paula Carvalho | Maia, Guilherme Oliveira | Rocha, Alice Caroline da Silva | Alves-Ribeiro, Bruna Samara | Zaiden, Letícia Caetano | Braga, Ísis Assis | Saturnino, Klaus Casaro | Ramos, Dirceu Guilherme de Souza | Gomes, Ana Paula Carvalho | Maia, Guilherme Oliveira | Rocha, Alice Caroline da Silva | Alves-Ribeiro, Bruna Samara | Zaiden, Letícia Caetano | Braga, Ísis Assis | Saturnino, Klaus Casaro | Ramos, Dirceu Guilherme de Souza
O crescimento exponencial das populações de animais domésticos tem consequências como o aumento da suscetibilidade a infecções por inúmeros parasitas. A giardíase, uma importante zoonose em cães e humanos causada por Giardia duodenalis, é transmitida pela via fecal-oral pela ingestão de alimentos e água contaminados com cistos ou por contato direto com humanos ou animais infectados. A falta de uma vacina aprovada comercialmente disponível para giardíase e o desenvolvimento de resistência aos antiparasitários devido ao uso incorreto e indiscriminado desses, tornam o tratamento um desafio significativo. Este estudo teve como objetivo relatar uma ocorrência anormalmente maior de Giardia spp. em cães de Jataí, Goiás, Brasil. Amostras fecais de 94 cães de diferentes idades e ambos os sexos foram adquiridas de uma clínica veterinária privada entre janeiro e setembro de 2022 com um surto relatado por veterinários em cães. O exame direto, flutuação simples e centrífugo sedimentação em solução de formalina-éter foram utilizados para identificar cistos e oocitos de parasitas protozoários e ovos de helmintos parasitas. Giardia spp. foi o parasita mais frequentemente detectado, com 20,21%, em contraste com as infecções por ancilostomídeos, com 12,77% (10/94), divergindo da prevalência relatada para esta localidade em estudos com amostras randomizadas: 29,53% para Ancylostoma spp. e 3,34% para Giardia spp. É importante ressaltar que as amostras do presente estudo foram de animais que já apresentavam sinais clínicos. No entanto, um estudo no local exato em Jataí mostrou que medicamentos antiparasitários são prescritos sem solicitação de exame coproparasitológico e até mesmo sem consulta ao médico veterinário, evidenciando o uso indiscriminado desses medicamentos. A maior ocorrência de Giardia spp. é evidente, e devido à importância da resistência aos medicamentos anti-helmínticos, este deve ser um fator a ser observado e investigado. Isso ressalta a necessidade de práticas veterinárias adequadas para prevenir a disseminação da giardíase e outras doenças zoonóticas. | The exponential growth of domestic animal populations has increased their susceptibility to infections caused by numerous parasites. Giardiasis, an important zoonosis in dogs and humans caused by Giardia duodenalis, is transmitted via the fecal-oral route by ingesting food and water contaminated with cysts or through direct contact with infected humans or animals. The lack of a commercially available approved vaccine for the treatment of giardiasis and the development of resistance to antiparasitics because of their incorrect and indiscriminate use make its treatment challenging. Herein, we report an abnormally high occurrence of Giardia spp. in dogs in veterinary care in Jataí, Goiás, Brazil. Fecal samples were acquired from 94 dogs of different ages and sexes in a private veterinary clinic between January and September 2022, with a giardiasis outbreak reported by veterinarians. Direct fecal examination, flotation, and centrifugal sedimentation in formalin ether solution were used to identify the cysts and oocysts of protozoan parasites and the eggs of parasitic helminths. Giardia spp. were the most frequently detected parasites (20.21%), as opposed to hookworm infections (12.77%, 10/94), which diverged from the prevalence reported for this locality in studies with randomized samples, being 29.53 and 3.34% for Ancylostoma spp. and Giardia spp., respectively. It is important to emphasize that the samples in the present study were from animals that had presented with clinical signs. However, a previous study conducted in the same locality in Jataí reported that antiparasitic drugs are prescribed without requesting a fecal examination and without consulting a veterinarian, evidencing the indiscriminate use of these drugs. The relatively high occurrence of Giardia spp. in the studied locality is evident. Considering growing anthelmintic drug resistance and the potential threats it may pose, the high occurrence of Giardia spp. is a factor that needs to be observed and investigated. The results obtained in this study highlight the need for appropriate veterinary practices to prevent the spread of giardiasis and other zoonotic diseases.
Afficher plus [+] Moins [-]Explorando o potencial das nanopartículas de prata para promover o desempenho de suínos: uma alternativa mais segura aos antibióticos | Harnessing silver nanoparticles to promote swine growth: a safer alternative to antibiotics Texte intégral
2025
Carnino, Bruno Braga | Alves, Laya Kannan Silva | Moraes, Emilye Izabele Cristina de | Madella, Gabriela dos Santos | Lange, Camila Neves | Pieretti, Joana Claudio | Tremiliosi, Guilherme Carvalho | Gonçalves, Joaquim Carlos Atra | Silveira, Hebert | Garbossa, Cesar Augusto Pospissil | Carnino, Bruno Braga | Alves, Laya Kannan Silva | Moraes, Emilye Izabele Cristina de | Madella, Gabriela dos Santos | Lange, Camila Neves | Pieretti, Joana Claudio | Tremiliosi, Guilherme Carvalho | Gonçalves, Joaquim Carlos Atra | Silveira, Hebert | Garbossa, Cesar Augusto Pospissil
Os antibióticos melhoradores de desempenho (AMD) têm sido amplamente utilizados na suinocultura. No entanto, seu uso apresenta implicações para a segurança sanitária global. As nanopartículas de prata (AgNPs) possuem propriedades antibióticas e podem representar uma alternativa viável. Neste estudo, AgNPs isoladas e incorporadas a um carboidrato (AgNPs@Carb) foram caracterizadas e testadas contra cepas bacterianas Gram-positivas e Gram-negativas. Para avaliar a ação das AgNPs@Carb como alternativa aos AMD, 32 suínos machos castrados e 32 fêmeas, com 70 dias de idade e peso médio de 24,08 ± 2,72 kg, foram alojados em uma unidade de crescimento e terminação. Os animais foram distribuídos em quatro tratamentos dietéticos: NC – sem adição de AgNPs@Carb; NP05 – 5 mg kg−1 de AgNPs@Carb; NP10 – 10 mg kg−1 de AgNPs@Carb; e NP15 – 15 mg kg−1 de AgNPs@Carb, com oito repetições de dois animais por baia. O experimento, com duração de 83 dias, foi dividido em duas fases: crescimento (0–42 dias) e terminação (43–83 dias). A inclusão de AgNPs@Carb na dieta aumentou o consumo médio diário de ração e o rendimento de carcaça resfriada, além de reduzir a frequência de diarreia e a espessura de toucinho dos animais. A dose ideal, estimada por regressão, foi de 7 mg kg−1. Esses resultados indicam que as AgNPs@Carb possuem potencial como aditivo alimentar na suinocultura, contribuindo para a redução do uso excessivo de antibióticos e promovendo uma produção mais segura e sustentável. | Antibiotics as growth promoters (AGP) have historically been a staple of the swine production industry. However, recent developments indicate that their use has several consequences for humanity’s sanitary safety. Silver nanoparticles (AgNPs) have antibiotic properties and could represent a viable alternative. In the current study, only AgNPs incorporated in a carbohydrate (AgNPs@Carb) were characterized and tested against Gram-positive and Gram-negative bacterial strains. To assess AgNPs@Carb action as an alternative to AGP, 32 barrows and 32 gilts were housed in a growing finishing facility with 70 days of age and an average weight of 24.08 ± 2.72 kg. They were fed four treatments: NC – no addition of AgNPs@Carb, NP05 - addition of 5 mg kg-1 of AgNPs@Carb, NP10 - 10 mg kg-1 of AgNPs@Carb, and NP15 - 15 mg kg-1 of AgNPs@Carb; each treatment had eight replicates of two animals (pen). The 83 days of the trial were divided into two periods: growing (0–42) and finishing (43–83). AgNPs@Carb addition to the diet improved average daily feed intake and chilled carcass yield and decreased diarrhea frequency and backfat thickness. AgNPs@Carb showed potential effects in acting as a feed additive in swine diets, and the ideal dose was estimated by regression at 7 mg kg-1. These findings offer a possible option to reduce the risk of antibiotics overuse, at least partially during swine feeding, for safer and cleaner production.
Afficher plus [+] Moins [-]Sacrococcygeal epidural administration of morphine and fentanyl inhorses: antinociceptive and physiological effects | Administração epidural sacrococcígea de morfina e fentanil em equinos: efeitos fisiológicos e antinociceptivos Texte intégral
2025
Damaceno, Andressa Brito | Souza, Maria Eduarda da Rocha | Bicalho, Amanda Moura | Oliveira, Otavio Pereira de | Reis, Rudiery Lauson de Paula | Cassani, Lais Sperandio | Lucas, Ester da Fonseca | Rosa, Hillary Ignez Belfi | Rocha, Poliana da Silva | Rego, Rafael Otaviano do | Trivilin, Leonardo Oliveira | Damaceno, Andressa Brito | Souza, Maria Eduarda da Rocha | Bicalho, Amanda Moura | Oliveira, Otavio Pereira de | Reis, Rudiery Lauson de Paula | Cassani, Lais Sperandio | Lucas, Ester da Fonseca | Rosa, Hillary Ignez Belfi | Rocha, Poliana da Silva | Rego, Rafael Otaviano do | Trivilin, Leonardo Oliveira
Opioids possess antinociceptive properties, and they are considered safe when administered via the epidural route. However, theyare seldom utilized in equine medicine. This study aimed to evaluate the physiological and antinociceptive effects of morphine and fentanyl, either individually or in combination, when administered via sacrococcygeal epidural catheterization in healthy horses. Five horses underwent sacrococcygeal epidural catheterization and received morphine (0.1 mg/kg) [M1], fentanyl (2 μg/kg) [M2], and a combination of morphine (0.15 mg/kg) and fentanyl (1 μg/kg) [M3]. Heart rate (HR), respiratory rate (RR), intestinal motility, rectal temperature (RT), sedation level, behavior, ataxia, and antinociceptive scores were recorded in response to thermals and mechanicals stimuli applied to the ischial and coxal tuberosities, as well as lumbar, tibial, and thoracic regions, at T-20’ (baseline), 15 min, 1 h, 4 h, 12 h, and 20 h. HR and RT remained within normal ranges. RR increased with [M1] (24.00 ± 2.83) and [M2] (25.20 ± 3.35) at T20h compared to baseline. Intestinal motility was modulated, notably reduced with morphine and its combination up to 1 h post administration, while fentanyl maintained stable bowel movements. Morphine alone or combined with fentanyl induced mild sedation up to 1 h post administration. Ataxia was notable for up to 4 h postadministration, especially with morphine, which extended ataxia up to 12 h. Antinociceptive scores increased notably between T1 and T4, with higher scores observed in the ischiatic, coxal, and lumbar dermatomes under cold stimulation, whereas all dermatomes exhibited increased scores under hot stimulation. Mechanical stimulation elicited antinociception in the coxal and lumbar dermatomes. In conclusion, fentanyl exhibited fewer physiological effects, while morphine demonstrated more potent antinociceptive effects during the initial 12-h period, with the combination showing intermediate analgesia. | Os opioides possuem propriedades antinociceptivas e são considerados seguros quando administrados pela via peridural. No entanto, eles raramente são utilizados na medicina equina. O objetivo deste estudo foi avaliar os efeitos fisiológicos e antinociceptivos de morfina e fentanil, isoladas ou em associação, quando administrados por cateter epidural sacrococcígeo em equinos saudáveis. Cinco equinos foram submetidos a cateterização epidural sacrococcígea e receberam morfina (0,1 mg/kg) [M1], fentanil (2 μg/kg) [M2] e a associação de morfina (0,15 mg/kg) e fentanil (1 μg/kg) [M3]. Frequência cardíaca (FC), frequência respiratória (FR), motilidade intestinal, temperatura retal (TR), nível de sedação, comportamento, ataxia e escores de antinocicepção foram registrados em resposta a estímulos térmicos e mecânicos aplicados às tuberosidades isquiática e coxal, bem como em regiões lombar, tibial e torácica, em T-20’ (baseline), 15 minutos, uma hora, quatro horas, 12 horas e 20 horas. FC e TR permaneceram dentro da normalidade. FR aumentou com [M1] (24.00 ± 2.83) e [M2] (25.20 ± 3.35) em T20h comparado ao baseline. A motilidade intestinal foi modulada, particularmente reduzida com morfina e sua combinação até uma hora após administração, enquanto o fentanil manteve movimentos intestinais estáveis. Morfina isolada ou combinada com fentanil induziu sedação leve até uma hora após administração. Ataxia foi notável por até quatro horas após administração, especialmente com morfina, que prolongou a ataxia por até 12 horas. Os escores antinociceptivos aumentaram notavelmente entre T1 e T4, com escores mais elevados observados nos dermátomos isquiáticos, coxais e lombares sob estímulo frio, enquanto sob estímulo quente todos os dermátomos exibiram escores aumentados. O estímulo mecânico provocou antinocicepção nos dermátomos coxais e lombares. Em conclusão, fentanil exibiu menos efeitos fisiológicos, enquanto morfina demonstrou efeitos antinociceptivos mais potentes durante o período inicial de 12 horas, com a combinação apresentando analgesia intermediária.
Afficher plus [+] Moins [-]Feasibility of ultrasound-guided cervical intervertebral disc injection to aid in intraoperative site identification in dog cadavers. Texte intégral
2025
Ficheroulle, Jérémie | Picavet, Pierre | Gommeren, Kris | Monseur, Justine | Bolen, Géraldine | FARAH. Médecine vétérinaire comparée - ULiège
peer reviewed | [en] OBJECTIVE: To study the feasibility of ultrasound-guided intervertebral disc (IVD) injection of contrast medium and methylene blue to aid in intraoperative identification of cervical IVD spaces in dogs. METHODS: This was a single-center experimental cadaver study using randomly chosen skeletally mature dog cadavers from January 2, 2023, to March 23, 2023. For each cadaver, 1 cervical IVD was randomly selected. The ventral cervical region was ultrasonographically examined, pushing the trachea leftwards. A 50/50 contrast medium (iohexol, 300 mgI/mL) and methylene blue mixture was injected under ultrasound guidance in the IVD and ventral soft tissues. The x-rays and CT scans were performed before and after injection. Each cadaver was dissected using a ventral slot procedure. The cadavers and IVD characteristics, the success rate in ultrasonographically identifying the correct IVD space, time to injection, most caudal IVD space feasibly injectable, and semiquantitative imaging and surgical scores were recorded. RESULTS: 20 canine cadavers were used. The IVD injections were successfully performed in the correct IVD space in all cadavers. The median time to injection was 3.95 minutes (Q1 to Q3, 3.22 to 5.88 minutes). The contrast medium was clearly visible on at least 1 radiographic projection in all but 1 case and on the CT in all cases. During surgical dissection, the dye was clearly visible in all but 1 case. The dogs' weight was significantly different between most caudal IVD spaces feasibly injectable. CONCLUSIONS: Ultrasonographical cervical IVD space identification and mixture injection are feasible and might help intraoperative cervical IVD space identification in dogs. CLINICAL RELEVANCE: This technique could be used presurgically in patients with cervical IVD diseases.
Afficher plus [+] Moins [-]Surgical management of severe tail injury by partial caudectomy in a long-tailed macaque (Macaca fascicularis) Texte intégral
2025
Sewoyo, Palagan | Wayan Wirata, I | Gorda, Wayan | Wardhita, Anak Agung Gde Jaya | Soma, I. Gede | Putra, I. Gusti Agung Arta | Wandia, I. Nengah | Brotcorne, Fany
peer reviewed | A male long-tailed macaque (Macaca fascicularis) was found to have a severe tail injury during a clinical examination prior to vasectomy at the Sacred Monkey Forest Sanctuary in Ubud, Bali, Indonesia. The injury involved an open wound with areas of necrosis and fracture. Based on the tail condition, the animal was treated with a partial caudectomy. Prophylactic treatment prior to surgery with antibiotic and anti-inflammatory was carried out using amoxicillin and ketorolac tromethamine, respectively. Anesthesia was maintained using propofol. Postoperatively, the anesthesia reversal agent atipamezole was administered. After the macaque exhibited clear signs of recovery from anesthesia, it was transported in a cage back to the capture site and released into its original social group. To the best of our knowledge, this is the first documented case report describing partial caudectomy in macaques.
Afficher plus [+] Moins [-]Domestic ferrets (Mustela putorius furo) with cholecystitis commonly show hyperbilirubinemia and common bile duct dilation, gallbladder sludge, and gallbladder wall thickening on ultrasound Texte intégral
2025
Basse, Catherine, Veciana | Ruel, Yannick | Agoulon, Albert | Gaillot, Hugues | ADVETIA Centre Hospitalier Vétérinaire | Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR) ; École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
International audience | OBJECTIVE To report clinical signs, biological anomalies, treatment, and outcome in domestic ferrets with cholecystitis, describe the ultrasonographic features of cholecystitis, and compare the ultrasonographic appearance of the hepatobiliary system between diseased and healthy ferrets. METHODS 11 ferrets with confirmed cholecystitis and abdominal ultrasonography were retrospectively included. Ten healthy ferrets were prospectively recruited to undergo hepatobiliary abdominal ultrasonography. Comparisons of explanatory variables (group, age, body weight, and sex) were performed. RESULTS The most common clinical signs were nonspecific, and icterus was observed in 1/4 of diseased ferrets. Compared with healthy individuals, diseased ferrets showed a larger common bile duct (CBD; ≥ 2.8 mm), a thicker CBD wall (≥ 0.7 mm), a thicker gallbladder (GB) wall, more frequent echoic GB luminal content, visible intrahepatic bile ducts, and a greater GB volume. Bile culture was positive in 9/11 mostly for Escherichia coli (8/9). Bile cytology indicated septic purulent cholecystitis in 7/7 ferrets. Survival time after antibiotic treatment ranged from 6 to 104 weeks, with a median of 36 weeks. CONCLUSIONS Ferrets with cholecystitis presented with nonspecific clinical signs and frequently showed hyperbilirubinemia. Ultrasonography findings such as enlarged CBD, thickened CBD wall, thicker GB wall, GB sludge, visible intrahepatic bile ducts, and enlarged GB may suggest cholecystitis. Escherichia coli was commonly isolated from bile. The medium-term mortality rate was high despite targeted antibiotherapy. CLINICAL RELEVANCE This is the first study describing the clinical signs, biological anomalies, ultrasonographic findings, and outcomes after treatment in a series of domestic ferrets with cholecystitis.
Afficher plus [+] Moins [-]Mapping the global scientific knowledge of peste des petits ruminants virus from 1990 to 2023: Identifying research hotspots, gaps, and future directions. Texte intégral
2025
Alzuheir, Ibrahim M
Objective: This study aims to analyze and map existing research literature on peste des petits ruminants virus (PPRV) to identify research hotspots, knowledge gaps, and future directions. PPRV causes peste des petits ruminants (PPRs) disease, leading to significant impacts on small ruminants through high mortality rates and trade limitations. Eradication programs are led by the Food and Agriculture Organization and the World Organization for Animal Health. Materials and Methods: Data were obtained from the Scopus database using keywords related to PPRV and PPR, covering the period from 1990 to 2023. Validation methods were employed to verify the search strategy’s accuracy. Data analysis focused on identifying temporal evolution, geographical distribution, key contributors, sources, research hotspots, and gaps, which were visualized using maps. Results: The study identified 478 research documents from 1,834 authors, with most being research articles (91.0%). A significant increase in publications was observed from 1990 to 2023, peaking in 2019 and 2021. India led with 112 articles, followed by the UK (79) and China (71). Top research institutions included the Indian Veterinary Research Institute and the Pirbright Institute in the UK. Major contributors like S. Parida and V. Balamurugan formed dense international collaboration networks. Key journals included “Transboundary and Emerging Diseases” and “Journal of Virological Methods.” Conclusion: This study reveals an increased global scientific production on PPRV, driven by international collaboration. However, research gaps remain, particularly from North African and Middle Eastern countries. Priorities include vaccine development, vaccination campaigns, veterinary capacity building, and enhanced reverse transcription polymerase chain reaction implementation. Insights from this study can guide policymakers, funders, and researchers in prioritizing resources and strategies to eradicate PPRV, ensuring sustainable livestock health and economic stability. J. Adv. Vet. Anim. Res., 12(1): 90–98, March 2025 http://doi.org/10.5455/javar.2025.l875
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