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Biochemical features of the introduced population of Serratula coronata L. (Asteraceae) in Central Polissia of Ukraine
2019
Іващенко, І. В | Рахметов, Д. Б | Вергун, О. М
Purpose. To establish the biochemical composition of the above ground part of Serratula coronata L. (Crowned saw-wort) for introduction in Central Polissia of Ukraine. Methods. The object of research was the plants of S. coronata from the collection of the Botanical garden of Zhytomyr National Agroecological University. Plant raw material was evaluated in the flowering phase in the biochemical laboratory of the Department of Cultural Flora of the M. M. Hryshko National Botanic Gardens of the National Academy of Sciences of Ukraine according to the relevant methods during 2014–2016. Results. The results of the study of phytochemical features of S. coronata under conditions of introduction in the Central Polissia of Ukraine in the flowering phase are given. Quantitative content of raw material in dry matter, total sugars, carotene, ascorbic acid, tannins, fats, free acids, macroelements of phosphorus, calcium and trace elements of iron, copper, zinc, manganese was revealed. Conclusions. The biochemical composition of the above ground part of S. coronata was determined for the first time in the conditions of introduction in Central Polissia of Ukraine. The peculiarities of the dependence of the content of biochemical compounds and macroelements on age characteristics of plants were determined. Plants of S. coronata of the third year of life were distinguished by the highest content of ascorbic acid, carotene and dry matter; two-year – organic acids, phosphorus, ash, common sugars; the fourth year of life – the content of oil and calcium. Significant amounts of vitamin C and iron were found in the raw material. The obtained results testify to the prospect of further study of the pharmacological properties of S. coronata in order to obtain new food products, bio-additives and phytopreparations enriched with biologically active substances and essential for human life.
Afficher plus [+] Moins [-]Study of some biochemical parameters and productivity of Silphium L. genotypes as perspective energetic crops
2020
Рахметов, Д. Б | Вергун, О. М | Рахметова, С. О | Шиманська, О. В | Фіщенко, В. В
Purpose. This study was aimed to determine some biochemical parameters and productivity of the gene fund of Silphium L. genus in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine. Methods. Plant raw material investigated at the flowering stage (17 genotypes) and the end of vegetation (20 genotypes) of Silphium spp. 3 species and 4 cultivars studied for the content of nutrients at the flowering. Determination of dry matter, ash, calcium, nitrogenfree extractives conducted according to Hrytsaienko et al. (2003), phosphorus, protein – according to Pochinok (1976), sugars – according to Krishchenko (1983), cellulose – according to Zheng et al. (2018), lipids – according to Zamowski, Suzuki (2004). It was used productivity parameters: yield of above-ground mass, the yield of dry mass, energetic value, yield of energy. Data analyzed statistically. Results. Investigation of nutrients content showed that content of dry matter was in the range of 18.90–29.3%, protein – in the range of 8.88–23.56%, cellulose – 15.10–36.14%, ash – 8.13–12.19%, lipids – 1.83–3.97%; yield of above-ground mass – 45.0–139.0 t/ha, the yield of dry matter – 10.31–36.92 t/ha, energy value – 3933–4249 cal/g, and yield of energy – 43.81–149.27 Gcal/ha. A study of genotypes at the flowering and end of vegetation identified that the content of dry matter for all samples was in a range of 18.38–67.49%, sugars – 2.78–19.0%, ash – 3.93–11.20%, calcium – 1.68–5.99%, phosphorus – 0.14–1.21%, energy value – 3153.36– 3770.28 cal/g. Conclusions. Plant raw material of genotypes of Silphium L. spp. is a valuable source of nutrients. The content of ash, its components, energetic value, and parameters of productivity depending on genotype and stage of growth. The results allow recommending selected Silphium genotypes as perspective energetic crops in Ukraine as well as other countries.
Afficher plus [+] Moins [-]Activation of growth and development of sugar beet at microstages 00-09 with application of nanoscale fertilizer elements
2019
Новицька, Н. В | Каленська, С. М | Присяжнюк, О. І | Мельниченко, В. В
Purpose. Finding ways to activate the germination of sugar beet seeds, obtaining even and synchronous sprouts when applying fertilizer compositions with nanoscale elements. Methods. Vegetation and laboratory. The seeds of sugar beet were sown in prepared utensils with soil in accordance with the requirements of the methods for vegetation experiments. Fertilizers were introduced in the form of solutions with different ratios according to six microstages. Results. At 01 microstage on the BBCH scale (130 hours after sowing), an increase in the mass of beet fruits in all variants was observed - in the control variant by 9.78%; in the application of nanofertilizers - 20,4-23,7%. The diameter of the fruit varied similarly to changes in mass: in the control variant, the diameter change was 4.95%; in variants with application of nanofertilizers — 9.56-13.9%. Changes in the rate of sprout organs formation and their linear dimensions were noted in the various fertilization schemes. The length of the embryonic root at 05 microstage with uniform introduction of high norms of zinc and phosphorus, after 40 hours after sowing, was 0.540-2.671 mm. For other fertilizer combinations, the appearance of the germ root was noted only 44 hours after sowing. In 60 hours after sowing (07 microstage on the BBCH scale) there was a complete exit of cotyledons from the socket of the cluster with the introduction of nano chelate microfertilizers and only the beginning of the exit of cotyledons in the control variant. Due to the intensive processes of swelling and germination, the growth of the primary root of the sugar beet was accelerated. Conclusions. Uniform provision of seeds with zinc and especially phosphorus on the background of basic complex fertilizer with nanoscale elements contributed to the activation of seed germination and the intense formation of synchronously developed shoots. On average, the opening of the pericarp lid and the appearance of the root accelerated for 4 hours; 6 hours earlier there was an exit of cotyledons. With the introduction of nano chelate fertilizers, root growth and elongation of the hypocotyl at the first microstages of sugar beet sprouting were accelerated twice, due to which the sugar beet sprouts appeared 4-6 hours earlier. Nano chelate microfertilizers, promoting even and synchronous germination, development of sugar beet seedlings ensured synchronous emergence of seedlings and formation of predetermined sowing density without further reduction of plants.
Afficher plus [+] Moins [-]Morphometric and biochemical features of different Bunias orientalis L. genotypes in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine
2021
Vergun, O. M. | Rakhmetov, D. B. | Shymanska, O. V. | Rakhmetova, S. O. | Bondarchuk, O. P. | Fishchenko, V. V.
Purpose. Determine a number of morphometric and biochemical parameters of various genotypes of Bunias orientalis L. in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG). Methods. Plant samples of B. orientalis (6 genotypes created in the NBG) were examined during the flowering stage. Determination of dry matter, ash, calcium was carried out according to Hrytsaienko et al. (2003), phosphorus according to Pochinok (1976), sugars, ascorbic acid and lipids were determined according to Krishchenko (1983), b-carotene according to Pleshkov (1985). The energy value of plants was determined using an IKA C-200 calorimeter. The obtained results were analysed statistically. Results. The height of plants varied from 140.9 (Genotype 1) to 157.5 (Genotype 5) cm, stem diameter from 11.67 (Genotype 1) to 16.1 (Genotype 6) mm, the number of internodes from 18.7 (Genotype 1) to 25.7 (Genotype 6), the number of leaves on a stem from 14.11 (Genotype 1) to 21.8 (Genotype 5), leaf lamina length from 14.2 (Genotype 1) to 23.45 (Genotype 6) cm, leaf lamina width from 6.34 (Genotype 1) to 14.5 (Genotype 4) cm, inflorescence length from 27.4 (Genotype 1) to 45.4 (Genotype 3) cm, inflorescence width from 2.32 (Genotype 1) to 4.92 (Genotype 3) cm, and the number of stems from 2.55 (Genotype 2) to 5.33 (Genotype 1). The study of the content of structural and functional compounds and nutrients at the flowering stage showed that the dry matter content was in the range of 13.58–16.00%, sugars 5.07–8.86%, titratable acidity 3.28–4.25%, lipids 3.33–6.61%, ascorbic acid 382.83–693.82 mg%, b-carotene 0.94–3.48 mg%, ash 6.79–9.2%, calcium 1.00–2.44%, phosphorus 1.61–2.67% and energy value 3337.0–3498.0 cal/g. Conclusions. It was revealed that samples of various genotypes of B. orientalis are a valuable source of nutrients at the flowering stage. The biochemical composition of plants depended on the genotype and stage of growth. Results of the morphometric study showed variability of investigated parameters. The obtained data can be used to predict and evaluate the results of introduction and breeding studies with B. orientalis genotypes as promising crops in Ukraine.
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