Affiner votre recherche
Résultats 1-3 de 3
Detection of genetically modified plants using LAMP (loop-mediated amplification) technologies
2021
Sorochynskyi, B. V.
Purpose. Analysis of the current state and experience on the loop-mediated amplification (LAMP) use to detect genetically modified plants. Methods. Literature search and analysis. Results. General information on the current state and use of the genetically modified plants is provided. Despite the wide distribution of genetically modified plants, the attitude towards them in society continues to remain somewhat wary. About 50 countries have introduced mandatory labeling of GM feed and products, provided that their content exceeds a certain threshold. In order to meet labeling requirements, effective and sensitive methods for detecting known genetic modifications in a variety of plant materials, food products and animal feed must be developed and standardized. The most common approaches to the detection of genetically modified organisms (GMOs) are the determination of specific proteins synthesized in transgenic plants and the detection of new introduced genes. Methods for the determination of GMOs based on the analysis of nucleic acids are more common, since such methods have greater sensitivity and specificity than the analysis of protein composition. Polymerase chain reaction (PCR) method is the main method of nucleic acid analysis, which is now wide used for the detection of GMOs. Loop-mediated amplification (LAMP), which can occur at a constant temperature and therefore does not require the use of expensive equipment may be an alternative to the PCR. Scientific articles about the use of the loop-mediated amplification (LAMP) for the detection of genetically modified plants were analyzed. Advantages and disadvantages of the polymerase chain reaction and loop-mediated amplification are compared. Conclusions. The main criteria for applying a method of GMO detection analysis are as follow: its sensitivity, time of reaction, availability and ease to use, cost of reagents and equipment, and the possibility for simultaneous detection of many samples.
Afficher plus [+] Moins [-]Analyzes of National Varietal Resources of Sugar Beet (Beta vulgaris L.)
2011
Мілієнко, М. В
The article considers national varietal sources of sugar beets, namely the progress of their generation, as well as general characteristics of productivity. Main trends in breeding reflecting the needs of society are highlighted. Importance of official qualifying examination for generating high-quality varietal diversity aimed at meeting the needs of national beetroot industry is emphasized.
Afficher plus [+] Moins [-]Origin of Analytical Breeding of Winter Wheat (Triticum aestivum L.) in the Tsar’s Russia, and in Ukraine in particular (End of XIX– Beginning of XX centuries)
2009
Піпан, Х. М
This article discusses the main factors of origin and development of analytical selection of winter wheat in Ukraine in late XIX - early XX century .. In particular highlight the role of scientific works of Charles Darwin, W. Rimpau, AL Sempolovskуу, IS Korzhinskуу and research institutions to increase attention to the study of local varieties of winter wheat and their improvement. Especially analyze values of Russia's first benefit from the breeding "Guide to Seed for the Advancement of cultivated plants", in which the author proposes methods for improving and creating new varieties. Since the end of XIX century selection were mainly involved in private owners on their own initiative, already in the beginning of XX century this trend more interested in the agricultural society, local and state authorities. Thanks to their support in the country organized congresses, meetings and conferences, set up research and field stations. It was during this period of transition took place in the national selection analysis.
Afficher plus [+] Moins [-]