Мета. Установити прояв комплексу ознак сортів обліпихи крушиноподібної, використовуючи кластерний аналіз для групування ідентифікаційних морфологічних якісних і кількісних господарсько-цінних характеристик за проведення тесту на відмінність. Методи. Аналітичний (аналіз бази даних сортів), порівняльного оцінювання морфологічних і господарсько-цінних характеристик, математичний, статистичний [використання пакета SPSS (статистичного пакета для соціальних наук, англ. Statistical Package for the Social Sciences)] інструментів Data Mining. Результати. Проведено частотний аналіз морфологічних ознак колекції сортів H. rhamnoides. Останні згруповано за допомогою ієрархічного агломеративного кластерного аналізу з використанням таких змінних, як життєва форма рослини, положення гілок і кількість колючок пагона. За результатами виокремлено шість кластерів. Також встановлено найліпші за господарсько-цінними показниками сорти обліпихи крушиноподібної в розрізі груп стиглості. Висновки. Використання кластерного аналізу для групування загальновідомих сортів колекції обліпихи крушиноподібної за морфологічними та господарстько-цінними характеристиками дає змогу оперативно та точно встановити код прояву ознак (QL – якісні, QN – кількісні, PQ – псевдоякісні) та їхню відмінність. За результатами порівняння встановлено, що найвищі показники врожайності мав сорт ‘Орендж Революшн’; вмісту вітаміну С ‒ ‘Дора’, каротину ‒ ‘Морквяна’, вітаміну Р ‒ ‘Татйана’, ‘Ласунка’, ‘Єва’ та ‘Марійа Брувеле’. | Purpose. To determine the manifestation of a complex of sea buckthorn (Hipporhae rhamnoides L.) varieties characteristics using cluster analysis for grouping of identifying morphological, qualitative and quantitative economic and valuable characteristics during the test of difference. Methods. Analytical (analysis of the variety database), comparative evaluation of morphological and economically valuable characteristics, mathematical, statistical [using the SPSS package (Statistical Package for the Social Sciences)] Data Mining tools. Results. The frequency analysis of morphological characters of the collection of H. rhamnoides varieties was carried out. The latter were grouped by means of hierarchical agglomerative cluster analysis using variables such as plant life form, branch position and number of shoot spines. Six clusters were identified from the results. The best sea buckthorn varieties in terms of economic value were also identified in terms of maturity groups. Conclusions. The use of cluster analysis to group known varieties of the sea buckthorn collection by morphological and economic characteristics allows to quickly and accurately establish the code of the characteristics (QL – qualitative, QN – quantitative, PQ – pseudo-qualitative) and their difference. According to the results of the comparison, the highest yields were found in the variety ‘Orange Revolution’; vitamin C content – ‘Dora’, carotene – ‘Morkviana’, vitamin P – ‘Tatiana’, ‘Lasunka’, ‘Eva’ and ‘Marija Bruvele’.

Мета. Використати кластерний аналіз морфологічних ознак для спрощення ідентифікації сортів Brassica oleracea var. italica та сформувати групи схожих сортів для тесту на відмінність. Методи. У процесі роботи послуговувалися аналітичним, математичним і статистичним методами. Як вхідну інформацію для статистичного опрацювання отриманих результатів застосовували відомості про результати експертизи на відмінність, однорідність і стабільність (ВОС) із бази даних автоматизованої інформаційної системи Українського інституту експертизи сортів рослин. Моделювання кластерів здійснювали за допомогою статистичного пакета IBM SPSS Statistics «Statistical Package for the Social Sciences». Результати. За 32 ознаками для тесту на відмінність, однорідність і стабільність проведено морфологічний опис сортів капусти броколі. Морфологічні кодові формули останніх, складені з відповідних кодів прояву ідентифікаційних ознак вегетативних і генеративних органів рослин, слугували джерелом вихідних даних. Серед 41 сорту, описаного за 32 морфологічними характеристиками, вдалося виокремити лише дві групи подібних за ідентифікаційними ознаками сортів. Як параметри моделі застосовували два типи змінних: цільова ‒ ознака «головка: антоціанове забарвлення», фокусна – «головка: забарвлення». Повний перелік характеристик був таким: «рослина: за висотою (за збиральної стиглості)», «листок: положення (на початку формування головки)», «листкова пластинка: хвилястість краю», «листкова пластинка: пухирчастість», «черешок: за довжиною», «головка: забарвлення», «головка: антоціанове забарвлення», «головка: за щільністю», «квітка: забарвлення», «квітка: інтенсивність жовтого забарвлення», «чоловіча стерильність». Способом комп’ютерного моделювання було сформовано кластери з 17 подібних сортів капусти броколі та 9 контрольних об’єктів (сортів), ідентифікація яких передбачала 11 морфологічних ознак. Висновки. Для пошуку відмітних ознак у процесі тесту на відмінність сорти капусти броколі було згруповано в кластери за такими морфологічними характеристиками, як положення листка на початку формування головки; хвилястість краю листкової пластинки; пухирчастість листкової пластинки; довжина черешка; забарвлення головки; наявність антоціанового та інтенсивність жовтого забарвлення. | Purpose. To use cluster analysis of morphological cha­racters to simplify the identification of Brassica oleracea var. italica and form groups of similar varieties for the test of difference. Methods. Analytical, mathematical and statistical methods were used in the work. As input information for the statistical processing of the obtained results, information on the results of the examination for distinctness, uniformity and stability (DUS) from the database of the Automated Information System of the Ukrainian Institute for Plant Varieties Examination was used. Cluster modelling was carried out using the IBM SPSS Statistics “Statistical Package for the Social Sciences”. Results. A morphological description of broccoli varieties was carried out on the basis of 32 characteristics for the examination of distinctness, uniformity and stability. The morphological code formulae of the latter, composed of the corresponding codes for the manifestation of identifying characteristics of vegetative and generative organs of plants, served as a source of initial data. Out of 41 varieties described by 32 morphological characteristics, only two groups were found to be similar in terms of the identifying characteristics of the varieties. Two types of variables were used as parameters of the model: target – characteristic “Head: anthocyanin colour”, focal – “Head: colour”. The full list of characteristics was as follows “plant: by height (at harvest maturity)”, “leaf: position (at beginning of head formation)”, “leaf blade: wavy edge”, “leaf blade: blistering”, “petiole: by length”, “head: colour”, “head: anthocyanin colour”, “head: by density”, “flower: colour”, “flower: intensity of yellow colour”, “male sterility”. Using computer modelling, clusters of 17 similar broccoli varieties and 9 control objects (varieties) were formed, the identification of which involved eleven morphological characteristics. Conclusions. In order to search for distinguishing characteristics in the process of testing the difference of cabbage varieties, broccoli was grouped into clusters according to such morphological characteristics as the position of the leaf at the beginning of the formation of the head; waviness of the edge of the leaf blade; blistering of the leaf plate; petiole length; head colour; presence of anthocyanin and intensity of yellow colour.

Purpose. To study the impact of clonal micropropagation and sanitation in vitro by viricide Ribavirin on ex vitro plant productivity, quantitative content and qualitative composition of peppermint essential oil components obtained from four breeding samples of peppermint plants (Mentha piperita L.) and the ‘Chornolysta’ variety. Methods. The study used methods of field agrotechnical one-factor experiment, essential oil distillation with water vapor according to the Ginzberg method, capillary gas chromatography and statistical analysis. Results. Due to the sanitation process in vitro, the yield of air-dried leaves of breeding samples increased by 2.9–7.1%, the ‘Chornolysta’ variety by 51.4% and rhizomes by 2.2–3.8% and 28.5% respectively, which amounted 0.35–2.74 t/ha. A significant increase of essential oil yield of breeding samples from 4.0 to 9.9 kg/ha was shown, and of the ‘Chornolysta’ variety – up to 28.6 kg/ha. After in vitro sanitation and clonal cropropagation of the breeding sample M 01-12, the content of essential oil was more than 4%. The following components of peppermint essential oil were identified: limonene, cineole, menton, mentofuran, isomenton, menthyl acetate, β-caryophyllene, isomenthol, menthol, pulegon, piperitone and carvone. A clear tendency to a decrease in the amount of menton and isomenthol with isomenton and menthol increase in plants, sanitated and propagated in vitro, was revealed. Conclusions. The use of tissue and organ culture methods and in vitro sanitation improves the qualitative composition of terpenoids by increasing the amount of menthol and menton reducing. The data obtained on the composition of terpenoids should be considered in peppermint selection as one of the integral criteria, which should be included in the list of economically valuable characteristics of peppermint plants, such as foliage, biomass of air-dried leaves, plant rhizomes and the amount of peppermint essential oil. Six indicators of the essential oil of the breeding sample M 01-02, namely citric acid, cineole, mentofuran, isomentone, pulegon, carvone, as well as the cineole / limonene ratio, meet the criteria of the European Pharmacopoeia, so it can be considered as promising for cultivation among the studied samples.

Purpose. To conduct in vitro screening of different genotypes of winter triticale for resistance to salinity in the shoot apical meristem culture. Methods. Plant tissue culture in vitro, in vitro breeding, statistical analysis. Results. It was found that the increase of sodium chloride concentration from 0.6 to 1.5% resulted in inhibition of the callus culture growth in all genotypes that was indicative of the toxic effect of the stress factor. It turns out that 1.2% sodium chloride concentration allowed to differentiate triticale genotypes for salt tolerance. The line ‘38/1296’ appeared to be the most resistant to salinity stress because under breeding conditions calli of this genotype were characterized by higher morphogenetic potential, had the highest crude mass increase, and plants-regenerants were obtained only from explants of this line after cultivation on the medium containing 1.5% sodium chloride. The ‘ADM 11’ variety was the most sensitive to saline stress as mass necrosis and lack of regenerative ability in its calli were observed under breeding conditions. In the studied forms, genotypic dependence of morphogenesis processes in vitro culture was registered. From the induced calli, plants-regenerants were obtained, and their completion of growing, root development and transfer to in vivo conditions were optimized. Conclusions. Genotypic response to salinity stress in the culture of shoot apical meristems of winter triticale was expressed by various crude mass increase and different morphogenetic potential on exposure to a stress factor. The line ‘38/1296’ can be used as a valuable material for further breeding of winter triticale. The culture of shoot apical meristems is recommended to apply as a test system for screening of triticale genotypes for resistance to salinity stress

Рurpose. To investigate inheritance of “plant height” trait in populations of the first (F1) and second (F2) generations of the soybean (Glucine max (L.) Merrill) and evaluate hybrid combinations in order to identify phenotypes with the highest level of heterosis for plant height. Methods. Laboratory test, mathematical and statistical analysis. Results. The inheritance of plant height in soybean was investigated. Hybrid combinations were assessed for the ability to select phenotypes with an optimal height. Different types of inheritance of this trait were found in populations of the second generation. The highest degree of heterosis for plant height was expressed by such soybean hybrids of the second generation as ‘АЕЕМ ’/‘Cherniatka’ (102.6%), next were ‘№ 427/‘Коrado’ (36,1%) and ‘Yelena’/‘Vizhion’ (32,0%). For the above trait, the ‘Legend’ variety in combinations with such varieties as ‘Staroukrainka’, ‘Korado’ and ‘Medeia’ expressed a heterosis amounting to 28.1%, 8.3%, 6.1% accordingly. Plant height was inherited mainly in terms of negative overdominance (34.2% combinations). There were 21.1% of combinations that had a negative semidominance. Conclusions. Among large majority of crossing combinations, forms were revealed that differed greatly for the plant height. The highest variability was observed in combinations such as ‘Legenda’/‘Korado’, ‘Ustia’/‘Vizjion’, ‘№894’/‘Vizjion’, ‘Ug-30’/‘Vizjion’, ‘Legenda’/‘Annushka’, ‘№441’/‘Gentleman’, ‘№441’/‘Vizjion’, ‘Yug-30’/‘Gentleman’, ‘Angelica’/‘Annushka’ and ‘Angelica’/‘Gentleman.

Purpose. To elaborate winter wheat cultivation technologies based on balanced fertilizer system that combines application of mineral fertilizers and the increase of their efficiency by the use of preparations with microelements. Methods. Field and laboratory studies, mathematical and statistical analysis. Results. During 2011–2013, the effect of cultivation technologies on the formation of yield and quality of winter wheat varie­ty ‘Benefis’ (pea is a predecessor) was studied. In case of alternative technologies that provided adding only by-products of the predecessor, the yield of winter wheat was 3.73 t/ha when using integrated protection system, and it was increased to 4.22 t/ha with grain quality of the 4th–5th class of the group B when foliar dressing was applied. Resource saving technologies of cultivation with restricted use of fertilizers (Р45К45N30(II)+30(IV)) provided productivity at the level of 5.19–5.61 t/ha with grain quality of the 2nd–3rd class of the group A. Grain yield of 6.27 t/ha of the 2nd class quality was obtained by the use of intensive cultivation technology, which included application of mineral fertilizers (Р90К90N30(II)+60(IV)+30(VIII)) in addition to the use of predecessor’s by-products and foliar dressing. The highest yield of grain (6.71 t/ha) on average during all years of the study with the 1st class of the group A quality was provided by energy-intensive technology, which included application of P135K135N60(II)+75(IV)+45(VIII) with embedding of predecessor’s by-products into the soil and foliar dressing. Conclusions. It was established that in the northern part of the Forest-Steppe zone of Ukraine the highest productivity of winter wheat was obtained in dark gray podzolic soils using the energy-intensive technology with application of P135K135N60(II)+75(IV)+45(VIII) against the background of predecessor’s by-products embedded into the soil in case of integrated plant protection, and foliar dres­sing. This technology ensured the yield of 6.71 t/ha with grain quality of the 1st class of the group A.

Purpose. To evaluate pea plants productivity and determine the degree of correlation among the main agronomic characters in pea varieties. Methods. Structural and statistical analysis. Results. Evaluation of pea samples in terms of indices of plant productivity elements level based on correlation analysis and single-factor indices appliance provided insight into the ratio of one trait share per unit of another one. It was defined that some correlations among the elements of productivity was not only moderate and weak, but they also changed their sign that could be the evidence of growth conditions influence on structural relationships bet­ween some traits and, consequently, redistribution of their contributions to the formation of variety productivity. Positive and very close relationship of many traits was revealed, particularly between plant height and the height of the plant up to the first bean, the number of nodes and the number of sterile nodes (r = 0,95–0,97). Methodological aspects of the variety model creation were considered, that may be useful not only in pea breeding but also for improving the techno­logy of its cultivation. Conclusions. Correlation relationships were established between the number of beans and the number of fruiting nodes and the number of carpophores containing 2 beans (r = 0,86–0,88), seed mass and plant mass (r = 0,81), the number of seeds per plant and plant mass and seed mass per plant (r = 0,78–0,81), the number of certified seeds and the number of seeds per plant (r = 0,84), the average number of beans per fertile node and the number of carpophores containing 2 beans (r = 0,74) that makes it possible to use them in assessing the productivity of plants.

Purpose. To develop and improve adaptive technologies of winter wheat cultivation which provide high productivity and quality of grain. Methods. Field and laboratory stu­dies, mathematical and statistical analysis. Results. During 2011–2015, the impact of cultivation technologies on the formation of grain quality and yield of winter wheat varie­ty ‘Stolychna’ (with pea as predecessor) was studied. When using alternative technologies with only predecessor by-products application, the yield of winter wheat with integ­rated crop protection system was 4,56 t/ha, with minimal protection – 4.25 t/ha with grain quality of the 5th class of B group. Resource saving cultivation technologies with limi­ted application of fertilizers (Р45К45N30(II)+30(IV)) provided productivity at the level of 4,87–5,50 t/ha with grain qua­lity of the 2nd–3rd class of A group. Grain yield of 6.01 t/ha with indicators of the 2nd–3rd class of quality was obtained with the use of intensive cultivation technology with application of mineral fertilizers (Р90К90N30(II)+60(IV)+30(VIII)) on the background of applying predecessor’s by-products and integra­ted crop protection. The highest yield of grain (6.22 t/ha) with indicators of the 2nd class of A group quality on average for the research period was provided by energy-intensive technology, that requires the application of mineral fertilize­rs (P135K135N60(II)+75(IV)+45(VIII)) and incorporation of predecessor’s by-products in the soil, and integrated plant protection. Conclusion. It was found that in the northern part of the Right-Bank Forest-Steppe zone of Ukraine, the highest productivity of winter wheat was obtained in dark grey podzolic soils when using the energy-intensive technology with application of P135K135N60(II)+75(IV)+45(VIII) on the background of predecessor’s by-products and integrated crop protection. This technology ensured the grain yield of 6.22 t/ha of the 2nd class of A group quality.

New donors of short-stem dominant characteristic Gnom 1 (G1), Gnom 2 (G2), Gnom 3 (G3) have been created to the purpose of further increasing the yield of Winter Rye and effective redistribution of assimilation products to the benefits of the grain. Genetic and Statistical analysis of 10 selective characteristics of these donors has been conducted and options to improve them were determined. Application of G1 in the prospective breeding programs G1 on 10 characteristics would not create considerable difficulties. Engagement in the crossing of G2 and G3 donors may create complications in the expression of- productivity characteristics of the ear and plants in the progenies. Crossing used in transgress and combinative breeding is preferable for applying. Application of G3 may be accompanied with technical inconvenience in perfor-ming directed crossing. Application of G3 attracts by the creating conditions for uniting desirable height of plant with the constitutionnal components of Winter Rye productivity.

Мета. Установити прояв комплексу ознак сортів обліпихи крушиноподібної, використовуючи кластерний аналіз для групування ідентифікаційних морфологічних якісних і кількісних господарсько-цінних характеристик за проведення тесту на відмінність. Методи. Аналітичний (аналіз бази даних сортів), порівняльного оцінювання морфологічних і господарсько-цінних характеристик, математичний, статистичний [використання пакета SPSS (статистичного пакета для соціальних наук, англ. Statistical Package for the Social Sciences)] інструментів Data Mining. Результати. Проведено частотний аналіз морфологічних ознак колекції сортів H. rhamnoides. Останні згруповано за допомогою ієрархічного агломеративного кластерного аналізу з використанням таких змінних, як життєва форма рослини, положення гілок і кількість колючок пагона. За результатами виокремлено шість кластерів. Також встановлено найліпші за господарсько-цінними показниками сорти обліпихи крушиноподібної в розрізі груп стиглості. Висновки. Використання кластерного аналізу для групування загальновідомих сортів колекції обліпихи крушиноподібної за морфологічними та господарстько-цінними характеристиками дає змогу оперативно та точно встановити код прояву ознак (QL – якісні, QN – кількісні, PQ – псевдоякісні) та їхню відмінність. За результатами порівняння встановлено, що найвищі показники врожайності мав сорт ‘Орендж Революшн’; вмісту вітаміну С ‒ ‘Дора’, каротину ‒ ‘Морквяна’, вітаміну Р ‒ ‘Татйана’, ‘Ласунка’, ‘Єва’ та ‘Марійа Брувеле’. | Purpose. To determine the manifestation of a complex of sea buckthorn (Hipporhae rhamnoides L.) varieties characteristics using cluster analysis for grouping of identifying morphological, qualitative and quantitative economic and valuable characteristics during the test of difference. Methods. Analytical (analysis of the variety database), comparative evaluation of morphological and economically valuable characteristics, mathematical, statistical [using the SPSS package (Statistical Package for the Social Sciences)] Data Mining tools. Results. The frequency analysis of morphological characters of the collection of H. rhamnoides varieties was carried out. The latter were grouped by means of hierarchical agglomerative cluster analysis using variables such as plant life form, branch position and number of shoot spines. Six clusters were identified from the results. The best sea buckthorn varieties in terms of economic value were also identified in terms of maturity groups. Conclusions. The use of cluster analysis to group known varieties of the sea buckthorn collection by morphological and economic characteristics allows to quickly and accurately establish the code of the characteristics (QL – qualitative, QN – quantitative, PQ – pseudo-qualitative) and their difference. According to the results of the comparison, the highest yields were found in the variety ‘Orange Revolution’; vitamin C content – ‘Dora’, carotene – ‘Morkviana’, vitamin P – ‘Tatiana’, ‘Lasunka’, ‘Eva’ and ‘Marija Bruvele’.