Purpose. The objective of this study was to evaluate the morphological parameters of Lycium spp. seeds from the collections in M. M. Hryshko National Botanical Garden (NBS) NAS of Ukraine. Methods. Cultivars and varieties of three Lycium species (Lycium barbarum, L. chinense, L. truncatum) were studied in the period from 2016 till 2019. The following morphometric measurments were conducted: seeds weight, seeds length, seeds width and index of seeds shape. Basic statistical analyses were performed using PAST 2.17. Hierarchical cluster analyses of similarity between genotypes were computed on the basis of the Bray-Curtis similarity index. Correlation between traits was determined using the Pearson correlation coefficient. Results. Cultivars and varieties of different species of Lycium varied in weight, shape, and size of seeds. Seed weight varied from 0.54 to 3.54 mg, seed length from 1.90 to 3.06 mm, seed width from 1.43 to 2.53 mm. The shape indexes of seeds were found ranging from 0.73 to 0.80. The analysis of coefficient of variation showed the difference of variability in morphometric characteristics between some Lycium spp. cultivars and varieties. The most variable features: seeds weight (8.51–28.22%) and seeds length (5.07–24.81%) are important parameters for selection. The use of cluster analysis made it possible to establish the similarity between the species of the studied Lycium species. Conclusions. Diagnostic signs by seed morphometry for differentiation of Lycium species were revealed. The analysis of coefficient of variation showed the difference of variability in morphometric characteristics between some Lycium cultivars and varieties. The most variable characteristics of the studied genotypes were seed weight and length, which are important parameters for selection because they determine the pulp content and number of seeds, as well as the ratio of these parameters between them. It is through variability that promising varieties with low seed weight and length can be selected, Due to securing them later vegetatively.

Purpose. The objective of this study was to evaluate the morphological parameters of Lycium spp. seeds from the collections in M. M. Hryshko National Botanical Garden (NBS) NAS of Ukraine. Methods. Cultivars and varieties of three Lycium species (Lycium barbarum, L. chinense, L. truncatum) were studied in the period from 2016 till 2019. The following morphometric measurments were conducted: seeds weight, seeds length, seeds width and index of seeds shape. Basic statistical analyses were performed using PAST 2.17. Hierarchical cluster analyses of similarity between genotypes were computed on the basis of the Bray-Curtis similarity index. Correlation between traits was determined using the Pearson correlation coefficient. Results. Cultivars and varieties of different species of Lycium varied in weight, shape, and size of seeds. Seed weight varied from 0.54 to 3.54 mg, seed length from 1.90 to 3.06 mm, seed width from 1.43 to 2.53 mm. The shape indexes of seeds were found ranging from 0.73 to 0.80. The analysis of coefficient of variation showed the difference of variability in morphometric characteristics between some Lycium spp. cultivars and varieties. The most variable features: seeds weight (8.51–28.22%) and seeds length (5.07–24.81%) are important parameters for selection. The use of cluster analysis made it possible to establish the similarity between the species of the studied Lycium species. Conclusions. Diagnostic signs by seed morphometry for differentiation of Lycium species were revealed. The analysis of coefficient of variation showed the difference of variability in morphometric characteristics between some Lycium cultivars and varieties. The most variable characteristics of the studied genotypes were seed weight and length, which are important parameters for selection because they determine the pulp content and number of seeds, as well as the ratio of these parameters between them. It is through variability that promising varieties with low seed weight and length can be selected, Due to securing them later vegetatively.

Purpose. The objective of this study was to evaluate the morphological parameters of Lycium spp. seeds from the collections in M. M. Hryshko National Botanical Garden (NBS) NAS of Ukraine.Methods. Cultivars and varieties of three Lycium species (Lycium barbarum, L. chinense, L. truncatum) were studied in the period from 2016 till 2019. The following morphometric measurments were conducted: seeds weight, seeds length, seeds width and index of seeds shape. Basic statistical analyses were performed using PAST 2.17. Hierarchical cluster analyses of similarity between genotypes were computed on the basis of the Bray-Curtis similarity index. Correlation between traits was determined using the Pearson correlation coefficient.Results. Cultivars and varieties of different species of Lycium varied in weight, shape, and size of seeds. Seed weight varied from 0.54 to 3.54 mg, seed length from 1.90 to 3.06 mm, seed width from 1.43 to 2.53 mm. The shape indexes of seeds were found ranging from 0.73 to 0.80. The analysis of coefficient of variation showed the difference of variability in morphometric characteristics between some Lycium spp. cultivars and varieties. The most variable features: seeds weight (8.51–28.22%) and seeds length (5.07–24.81%) are important parameters for selection. The use of cluster analysis made it possible to establish the similarity between the species of the studied Lycium species.Conclusions. Diagnostic signs by seed morphometry for differentiation of Lycium species were revealed. The analysis of coefficient of variation showed the difference of variability in morphometric characteristics between some Lycium cultivars and varieties. The most variable characteristics of the studied genotypes were seed weight and length, which are important parameters for selection because they determine the pulp content and number of seeds, as well as the ratio of these parameters between them. It is through variability that promising varieties with low seed weight and length can be selected, Due to securing them later vegetatively. | Мета. Оцінити морфометричні показники насіння Lycium spp. колекції Національного ботанічного саду імені М. М. Гришка (НБС) НАН України.Методи. Упродовж 2016–2019 рр. досліджено 21 генотип (10 сортів і 11 форм) трьох видів (Lycium barbarum, L. chinense, L. truncatum). Установлено морфометричні показники насіння (маса, довжина, ширина та індекс форми). Статистичний аналіз виконували за допомогою PAST 2.17. Ієрархічний кластерний аналіз подібності генотипів здійснено за індексом подібності Брей-Кертіса. Наявніть зв’язків між параметрами встановлювали за коефіцієнтом кореляції Пірсона.Результати. Сорти та форми різних видів рослин Lycium варіювали за масою, розміром та формою насіння. Морфометричні параметри насіння були такими: маса – від 0,54 до 3,54 мг, довжина – від 1,90 до 3,06 мм, ширина – від 1,43 до 2,53 мм. Величина індексу форми насіння становила від 0,73 до 0,80. Аналіз коефіцієнта варіації показав різну мінливість морфометричних характеристик сортів та форм різних видів Lycium. Наймінливішою є маса насіння (8,51–28,22%) та його довжина (5,07–24,81%), які є важливими параметрами для селекції. Використання кластерного аналізу дало змогу встановити генетичні зв’язки між сортами й формами Lycium та розподілити їх у два основних кластери.Висновки. Виявлено діагностичні ознаки морфометричних параметрів насіння для ідентифікації видів Lycium. Аналіз коефіцієнта варіації показав мінливість морфомет­ричних характеристик між деякими сортами та формами Lycium. Наймінливішими параметрами досліджуваних генотипів були маса та довжина насіння. Останні є важливими для селекції, оскільки від них залежить уміст м’якушу та кількість насіння, а також співвідношення цих параметрів між ними. Завдяки мінливості можна дібрати перспективні сорти з невеликими масою та довжиною насіння, закріпивши їх потім вегетативно.

Purpose. Determine the ecological plasticity and productivity of F1 sunflower hybrids created on the basis of maternal and parental lines, selected according to an accelerated selection system of lines resistant to herbicides (imidazoline and sulfonylurea groups) and broomrape (Orobanche cumana Wallr.). Methods. Statistical analysis of F1 sunflower hybrids was carried out using the methods of variation statistics, regression and analysis of variance using the Microsoft Office Excel 2016 application package. Molecular biological, biotechnological and classical selection methods were used for the accelerated system of line selection. Thus, for the purpose of targeted selection of sunflower sterility fixers, we used HRG01 molecular SCAR marker to identify the gene for the restoration of pollen fertility (Rf1). To accelerate the creation of parental lines resistant to tribenuron-methyl, we used a culture of immature embryos in vitro. Results. The results of testing of F1 sunflower hybrids at Kyiv, Chernihiv, Cherkasy (Uman and Shpolianskyi districts), Khmelnytskyi, Kharkiv, Kherson and Odesa regions. The hybrids were created on the basis of selected lines, chosen according to an accelerated selection system for herbicide-resistant lines (imidazoline (IMI-hybrids) and sulfonylurea (SU-hybrids) groups) and broomrape (Orobanche cumana Wall). The standards for comparison with hybrids were: for IMI hybrids – hybrids ‘NK Neoma’ (Syngenta) and ‘ES Genesis’ (Euralis), and for SU-hybrids – ‘SY Sumiko’ (Syngenta) and ‘P64LE25’ (Pioneer). As a result, it was found that among SU-hybrids, UA 2/106 had a 3.9% higher yield when compared to the standards (‘SY Sumiko’ and ‘P64LE25’). And for IMI-hybrids it was found that hybrids UA 1/67, UA 1/66, UA 1/84 have the same yield of 2.76 t/ha as the ‘NK Neoma’ standard. IMI hybrids UA 1/92, UA 1/102 have the same yield of 2.91 t/ha as ‘ES Genesis’. Conclusions. F1 hybrids were created on the basis of the original breeding material selected due to the accelerated system of sunflower lines selection. The hybrids were analyzed according to the yield indicator. The most productive among the tested SU-hybrids was UA 2/106 hybrid, among the IMI hybrids – UA 1/67, UA 1/66, UA 1/84, UA 1/92 and UA 1/102.

Purpose. Determine the ecological plasticity and productivity of F1 sunflower hybrids created on the basis of maternal and parental lines, selected according to an accelerated selection system of lines resistant to herbicides (imidazoline and sulfonylurea groups) and broomrape (Orobanche cumana Wallr.). Methods. Statistical analysis of F1 sunflower hybrids was carried out using the methods of variation statistics, regression and analysis of variance using the Microsoft Office Excel 2016 application package. Molecular biological, biotechnological and classical selection methods were used for the accelerated system of line selection. Thus, for the purpose of targeted selection of sunflower sterility fixers, we used HRG01 molecular SCAR marker to identify the gene for the restoration of pollen fertility (Rf1). To accelerate the creation of parental lines resistant to tribenuron-methyl, we used a culture of immature embryos in vitro. Results. The results of testing of F1 sunflower hybrids at Kyiv, Chernihiv, Cherkasy (Uman and Shpolianskyi districts), Khmelnytskyi, Kharkiv, Kherson and Odesa regions. The hybrids were created on the basis of selected lines, chosen according to an accelerated selection system for herbicide-resistant lines (imidazoline (IMI-hybrids) and sulfonylurea (SU-hybrids) groups) and broomrape (Orobanche cumana Wall). The standards for comparison with hybrids were: for IMI hybrids – hybrids ‘NK Neoma’ (Syngenta) and ‘ES Genesis’ (Euralis), and for SU-hybrids – ‘SY Sumiko’ (Syngenta) and ‘P64LE25’ (Pioneer). As a result, it was found that among SU-hybrids, UA 2/106 had a 3.9% higher yield when compared to the standards (‘SY Sumiko’ and ‘P64LE25’). And for IMI-hybrids it was found that hybrids UA 1/67, UA 1/66, UA 1/84 have the same yield of 2.76 t/ha as the ‘NK Neoma’ standard. IMI hybrids UA 1/92, UA 1/102 have the same yield of 2.91 t/ha as ‘ES Genesis’. Conclusions. F1 hybrids were created on the basis of the original breeding material selected due to the accelerated system of sunflower lines selection. The hybrids were analyzed according to the yield indicator. The most productive among the tested SU-hybrids was UA 2/106 hybrid, among the IMI hybrids – UA 1/67, UA 1/66, UA 1/84, UA 1/92 and UA 1/102.

Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the lifespan of grapevine. Considering the impact and the spread of these diseases, our objective was to analyse the presence of these two viruses in several grapevine varieties in grapevine collection at ATTC Vlore. Data gathered from plant pathogens serve to better understand and prevent the spread of pathogens, as a mandatory rule for the quality control of certified plant material during vegetative propagation. Method. The presence of two common viruses were tested using virus specific primers; LC1/LC2 primer pair designed from the hHSP70 gene for detecting Grapevine Leafroll-associated Virus-3 (GLRaV3) and C3390/H2999 primer pair, designed from coat protein coding regions for detecting Grapevine Fanleaf Virus (GFLV), in six varieties; ‘Merlot’, ‘Kallmet’, ‘Shesh i zi’, ‘Shesh i bardhё’, ‘Debinё’, and ‘Pulёz’, provided through a randomised sampling procedure. One Step Reverse Transcription Polymerase Chain Reaction assay was used to detect the viral presence. Results showed a high (100%) prevalence of GLRaV3 virus in all of analysed samples, as the most frequent among the two pathogens. Analysis for of GFLV virus showed low infection rate, being present in only one sample. Conclusions. We herein show an efficient, fast and reproducible method for detecting grapevine viruses through one step RT-PCR. Our results suggest that sampling of the infected plant material should be avoided due to the presence of viral infections.

Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the lifespan of grapevine. Considering the impact and the spread of these diseases, our objective was to analyse the presence of these two viruses in several grapevine varieties in grapevine collection at ATTC Vlore. Data gathered from plant pathogens serve to better understand and prevent the spread of pathogens, as a mandatory rule for the quality control of certified plant material during vegetative propagation. Method. The presence of two common viruses were tested using virus specific primers; LC1/LC2 primer pair designed from the hHSP70 gene for detecting Grapevine Leafroll-associated Virus-3 (GLRaV3) and C3390/H2999 primer pair, designed from coat protein coding regions for detecting Grapevine Fanleaf Virus (GFLV), in six varieties; ‘Merlot’, ‘Kallmet’, ‘Shesh i zi’, ‘Shesh i bardhё’, ‘Debinё’, and ‘Pulёz’, provided through a randomised sampling procedure. One Step Reverse Transcription Polymerase Chain Reaction assay was used to detect the viral presence. Results showed a high (100%) prevalence of GLRaV3 virus in all of analysed samples, as the most frequent among the two pathogens. Analysis for of GFLV virus showed low infection rate, being present in only one sample. Conclusions. We herein show an efficient, fast and reproducible method for detecting grapevine viruses through one step RT-PCR. Our results suggest that sampling of the infected plant material should be avoided due to the presence of viral infections.

Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the lifespan of grapevine. Considering the impact and the spread of these diseases, our objective was to analyse the presence of these two viruses in several grapevine varieties in grapevine collection at ATTC Vlore. Data gathered from plant pathogens serve to better understand and prevent the spread of pathogens, as a mandatory rule for the quality control of certified plant material during vegetative propagation. Method. The presence of two common viruses were tested using virus specific primers; LC1/LC2 primer pair designed from the hHSP70 gene for detecting Grapevine Leafroll-associated Virus-3 (GLRaV3) and C3390/H2999 primer pair, designed from coat protein coding regions for detecting Grapevine Fanleaf Virus (GFLV), in six varieties; ?Merlot?, ?Kallmet?, ?Shesh i zi?, ?Shesh i bardh??, ?Debin??, and ?Pul?z?, provided through a randomised sampling procedure. One Step Reverse Transcription Polymerase Chain Reaction assay was used to detect the viral presence. Results showed a high (100%) prevalence of GLRaV3 virus in all of analysed samples, as the most frequent among the two pathogens. Analysis for of GFLV virus showed low infection rate, being present in only one sample. Conclusions. We herein show an efficient, fast and reproducible method for detecting grapevine viruses through one step RT-PCR. Our results suggest that sampling of the infected plant material should be avoided due to the presence of viral infections. | ????. ??????? ????????? (Vitis spp.) ?????????? ???????? ????????? ??????????, ?? ??????????? ???? ???????? ????????????. ?? ?????????????? ???????? ????? ??????????? ????? ????????? (GLRaV-3) ?? ????? ???????????? ????????? (GFLV), ????????????? ???????, ???? ??????? ?????????? ????? ??????????? ????. ? ?????? ?? ?????????? ? ????????? ???????????, ?? ????????????? ????????? ????????, ????? ????? ???? ?????????????? ???? ??????????? ? ?????? ????????? ? ???????? ?????? ????????? ??????? ?????????? (ATTC Vlore). ???????? ???? ??? ???????? ???????? ???????? ??? ??????????? ??????? ????????? ? ? ????????????? ??? ????????????? ?????? ??????????????? ?????????? ????????? ??? ??? ???? ????????????? ???????????. ??????. ????????? ??????? ?????????? ??????? ????????????? ??-??? ? ????????????? ?????-??????????? ?????????: ???? ????????? LC1 / LC2, ?? ?????????? ??? ???????????? ???? hHSP70 ?????? ??????????? ????? ??????????-3 (GLRaV3), ? ???? ????????? C3390 / H2999, ??? ?????????? ??????????? ?????????????? ????? ???????? ?????? ???????????? ????????? (GFLV). ?????? ????? ?????? ???????? ? ?Merlot?, ?Kallmet?, ?Shesh i zi?, ?Shesh i bardh??, ?Debin?? ? ?Pul?z? ? ??????????? ? ????????????? ????????? ?????????????? ???????. ??????????. ?????????????? ??????? ??? ??????????? ??????? ???????? GLRaV3, ???? ?????????? ? 100% ??????????????? ??????. ?????????? ?????? GFLV ???????? ??????? ?????? ???????????, ????? ??? ???? ? ?????? ??????. ????????. ???????? ?????????? ???????????? ????????????? ??-??? ?? ???????????, ???????? ? ?????????????? ?????? ????????? ??????? ??????????? ????.

Purpose. Jute mallow (Corchorus olitorius L.) is a mucilaginous vegetable and fiber crop cultivated in the tropics, where catnip (Nepeta cataria L.) and Mexican sunflower (Tithonia diversifolia L.) are common weeds. Hence, the study investigated the growth, yield, and nutrient level of jute mallow in weed-free, catnip, and Mexican sunflower environments. Methods. The study involved two screen-house experiments in a Completely Randomized Design (CRD) with six replications. The treatments were 0 (control), 2, 4, 6, 8, and 10 weed plants per pot in both experiments. These are 0, 100, 200, 300, 400, and 500 weed count per square meter equivalent, based on the surface area of the pots used. Mexican sunflower and catnip plants interacted with jute plants in the first and second experiments, respectively. Growth parameters of jute mallow were recorded weekly from 5 to 8 weeks after sowing (WAS), and harvesting was done at 8 WAS. The proximate composition of jute was evaluated using standard procedures outlined by AOAC. The data collected were subjected to analysis of variance (ANOVA), and means were separated using Duncan Multiple Range Test (DMRT) at P < 0.05. Results. Catnip and Mexican sunflower negatively impacted the morphological features of jute mallow from 100 plants per square meter upwards. Catnip and Mexican sunflower, at 300 and 100–500 plants per square meter, respectively, reduced the dry weight of jute mallow. The crude protein content of jute mallow was also lessened by Mexican sunflower at some point. Conclusions. The study recommends that the density of catnip and Mexican sunflower plants interacting with jute mallow should be maintained below 100 plants per square meter to prevent yield loss.

Purpose. Jute mallow (Corchorus olitorius L.) is a mucilaginous vegetable and fiber crop cultivated in the tropics, where catnip (Nepeta cataria L.) and Mexican sunflower (Tithonia diversifolia L.) are common weeds. Hence, the study investigated the growth, yield, and nutrient level of jute mallow in weed-free, catnip, and Mexican sunflower environments. Methods. The study involved two screen-house experiments in a Completely Randomized Design (CRD) with six replications. The treatments were 0 (control), 2, 4, 6, 8, and 10 weed plants per pot in both experiments. These are 0, 100, 200, 300, 400, and 500 weed count per square meter equivalent, based on the surface area of the pots used. Mexican sunflower and catnip plants interacted with jute plants in the first and second experiments, respectively. Growth parameters of jute mallow were recorded weekly from 5 to 8 weeks after sowing (WAS), and harvesting was done at 8 WAS. The proximate composition of jute was evaluated using standard procedures outlined by AOAC. The data collected were subjected to analysis of variance (ANOVA), and means were separated using Duncan Multiple Range Test (DMRT) at P < 0.05. Results. Catnip and Mexican sunflower negatively impacted the morphological features of jute mallow from 100 plants per square meter upwards. Catnip and Mexican sunflower, at 300 and 100–500 plants per square meter, respectively, reduced the dry weight of jute mallow. The crude protein content of jute mallow was also lessened by Mexican sunflower at some point. Conclusions. The study recommends that the density of catnip and Mexican sunflower plants interacting with jute mallow should be maintained below 100 plants per square meter to prevent yield loss.

Purpose. Jute mallow (Corchorus olitorius L.) is a mucilaginous vegetable and fiber crop cultivated in the tropics, where catnip (Nepeta cataria L.) and Mexican sunflower (Tithonia diversifolia L.) are common weeds. Hence, the study investigated the growth, yield, and nutrient level of jute mallow in weed-free, catnip, and Mexican sunflower environments.Methods. The study involved two screen-house experiments in a Completely Randomized Design (CRD) with six replications. The treatments were 0 (control), 2, 4, 6, 8, and 10 weed plants per pot in both experiments. These are 0, 100, 200, 300, 400, and 500 weed count per square meter equivalent, based on the surface area of the pots used. Mexican sunflower and catnip plants interacted with jute plants in the first and second experiments, respectively. Growth parameters of jute mallow were recorded weekly from 5 to 8 weeks after sowing (WAS), and harvesting was done at 8 WAS. The proximate composition of jute was evaluated using standard procedures outlined by AOAC. The data collected were subjected to analysis of variance (ANOVA), and means were separated using Duncan Multiple Range Test (DMRT) at P < 0.05.Results. Catnip and Mexican sunflower negatively impacted the morphological features of jute mallow from 100 plants per square meter upwards. Catnip and Mexican sunflower, at 300 and 100?500 plants per square meter, respectively, reduced the dry weight of jute mallow. The crude protein content of jute mallow was also lessened by Mexican sunflower at some point.Conclusions. The study recommends that the density of catnip and Mexican sunflower plants interacting with jute mallow should be maintained below 100 plants per square meter to prevent yield loss. | ????. ???? ???????????? (Corchorus olitorius L) ? ?? ??????? ? ??????? ????????, ??? ????????? ? ????????. ?????????? ?????????, ?? ?????????? ???? ??????, ? ?????? ????? ???????? (Nepeta cataria L.) ? ????????????? ???????? (Tithonia diversifolia?L). ? ?????? ??????????? ??????? ????, ??????????? ? ????? ?????? ??????????? ? ???????? ????? ????????????? ?? ????? ??????????? ??????? ????? ?????????? ? ?????????????? ????????? ?? ? ??????????, ???????? ??? ????????.??????. ??????????? ???????? ??? ???????????? ???????????? ?? ???????? ?????????????? ?????? ? ????? ???????????. ????? ??????? ? ????????????? ???? ?????: 0 (????????), 2, 4, 6, 8 ? 10 ???????? ?? ??????? ??? ???????????. ? ??????????? ????? ???????? ???????????? ????????, ???? ????????? ??????????? 0, 100, 200, 300, 400 ? 500 ???????? ?? 1??2. ????????? ????? ????? ????????????? ??????????? ??????? ? 5 ?? 8 ????? ????? ?????, ? ?????? ??????? ????? 8 ??????. ????? ?????? ?????? ? ???????? ????? ????????? ? ????????????? ??????????? ????????, ?????????? ?????????? ????????? ????????????????????? ???????. ???????? ?????????? ???? ????????? ?? ????????????? ??????? ????????????? ??????? (ANOVA).??????????. ?????? ????? ???????? ? ?????????????? ???????? ?? ????????? ??? 100 ?????? ?? ???????????? ???? ??????? ????????? ???????? ?? ???????????? ???????????????? ????? ?????????????. ?? ???????, ?? ??????????? 100?500 ?????? ?? ?????????? ????, ??????????? ????? ????????? ????? ???? ????? ?????????????. ?? ????????????, ????????????? ???????? ???? ????? ??????????? ????????? ?????? ?????? ???????? ? ????? ?????????????.????????. ??? ??????????? ????? ?????? ?????????????? ???????????? ??????????? ?????? ??????? ????? ??????????? ?? ?????????????? ????????? ? ??????? ????? ??????????????? ?????? ??? 100 ??./?2.

Purpose. Determine a number of morphometric and biochemical parameters of various genotypes of Bunias orientalis L. in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG). Methods. Plant samples of B. orientalis (6 genotypes created in the NBG) were examined during the flowering stage. Determination of dry matter, ash, calcium was carried out according to Hrytsaienko et al. (2003), phosphorus according to Pochinok (1976), sugars, ascorbic acid and lipids were determined according to Krishchenko (1983), b-carotene according to Pleshkov (1985). The energy value of plants was determined using an IKA C-200 calorimeter. The obtained results were analysed statistically. Results. The height of plants varied from 140.9 (Genotype 1) to 157.5 (Genotype 5) cm, stem diameter from 11.67 (Genotype 1) to 16.1 (Genotype 6) mm, the number of internodes from 18.7 (Genotype 1) to 25.7 (Genotype 6), the number of leaves on a stem from 14.11 (Genotype 1) to 21.8 (Genotype 5), leaf lamina length from 14.2 (Genotype 1) to 23.45 (Genotype 6) cm, leaf lamina width from 6.34 (Genotype 1) to 14.5 (Genotype 4) cm, inflorescence length from 27.4 (Genotype 1) to 45.4 (Genotype 3) cm, inflorescence width from 2.32 (Genotype 1) to 4.92 (Genotype 3) cm, and the number of stems from 2.55 (Genotype 2) to 5.33 (Genotype 1). The study of the content of structural and functional compounds and nutrients at the flowering stage showed that the dry matter content was in the range of 13.58–16.00%, sugars 5.07–8.86%, titratable acidity 3.28–4.25%, lipids 3.33–6.61%, ascorbic acid 382.83–693.82 mg%, b-carotene 0.94–3.48 mg%, ash 6.79–9.2%, calcium 1.00–2.44%, phosphorus 1.61–2.67% and energy value 3337.0–3498.0 cal/g. Conclusions. It was revealed that samples of various genotypes of B. orientalis are a valuable source of nutrients at the flowering stage. The biochemical composition of plants depended on the genotype and stage of growth. Results of the morphometric study showed variability of investigated parameters. The obtained data can be used to predict and evaluate the results of introduction and breeding studies with B. orientalis genotypes as promising crops in Ukraine.

Purpose. Determine a number of morphometric and biochemical parameters of various genotypes of Bunias orientalis L. in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG). Methods. Plant samples of B. orientalis (6 genotypes created in the NBG) were examined during the flowering stage. Determination of dry matter, ash, calcium was carried out according to Hrytsaienko et al. (2003), phosphorus according to Pochinok (1976), sugars, ascorbic acid and lipids were determined according to Krishchenko (1983), b-carotene according to Pleshkov (1985). The energy value of plants was determined using an IKA C-200 calorimeter. The obtained results were analysed statistically. Results. The height of plants varied from 140.9 (Genotype 1) to 157.5 (Genotype 5) cm, stem diameter from 11.67 (Genotype 1) to 16.1 (Genotype 6) mm, the number of internodes from 18.7 (Genotype 1) to 25.7 (Genotype 6), the number of leaves on a stem from 14.11 (Genotype 1) to 21.8 (Genotype 5), leaf lamina length from 14.2 (Genotype 1) to 23.45 (Genotype 6) cm, leaf lamina width from 6.34 (Genotype 1) to 14.5 (Genotype 4) cm, inflorescence length from 27.4 (Genotype 1) to 45.4 (Genotype 3) cm, inflorescence width from 2.32 (Genotype 1) to 4.92 (Genotype 3) cm, and the number of stems from 2.55 (Genotype 2) to 5.33 (Genotype 1). The study of the content of structural and functional compounds and nutrients at the flowering stage showed that the dry matter content was in the range of 13.58–16.00%, sugars 5.07–8.86%, titratable acidity 3.28–4.25%, lipids 3.33–6.61%, ascorbic acid 382.83–693.82 mg%, b-carotene 0.94–3.48 mg%, ash 6.79–9.2%, calcium 1.00–2.44%, phosphorus 1.61–2.67% and energy value 3337.0–3498.0 cal/g. Conclusions. It was revealed that samples of various genotypes of B. orientalis are a valuable source of nutrients at the flowering stage. The biochemical composition of plants depended on the genotype and stage of growth. Results of the morphometric study showed variability of investigated parameters. The obtained data can be used to predict and evaluate the results of introduction and breeding studies with B. orientalis genotypes as promising crops in Ukraine.

Purpose. Determine a number of morphometric and biochemical parameters of various genotypes of Bunias orientalis L. in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG).Methods. Plant samples of B. orientalis (6 genotypes created in the NBG) were examined during the flowering stage. Determination of dry matter, ash, calcium was carried out according to Hrytsaienko et al. (2003), phosphorus according to Pochinok (1976), sugars, ascorbic acid and lipids were determined according to Krishchenko (1983), b-carotene according to Pleshkov (1985). The energy value of plants was determined using an IKA C-200 calorimeter. The obtained results were analysed statistically.Results. The height of plants varied from 140.9 (Genotype 1) to 157.5 (Genotype 5) cm, stem diameter from 11.67 (Genotype 1) to 16.1 (Genotype 6) mm, the number of internodes from 18.7 (Genotype 1) to 25.7 (Genotype 6), the number of leaves on a stem from 14.11 (Genotype 1) to 21.8 (Genotype 5), leaf lamina length from 14.2 (Genotype 1) to 23.45 (Genotype 6) cm, leaf lamina width from 6.34 (Genotype 1) to 14.5 (Genotype 4) cm, inflorescence length from 27.4 (Genotype 1) to 45.4 (Genotype 3) cm, inflorescence width from 2.32 (Genotype 1) to 4.92 (Genotype 3) cm, and the number of stems from 2.55 (Genotype 2) to 5.33 (Genotype 1). The study of the content of structural and functional compounds and nutrients at the flowering stage showed that the dry matter content was in the range of 13.58–16.00%, sugars 5.07–8.86%, titratable acidity 3.28–4.25%, lipids 3.33–6.61%, ascorbic acid 382.83–693.82 mg%, b-carotene 0.94–3.48 mg%, ash 6.79–9.2%, calcium 1.00–2.44%, phosphorus 1.61–2.67% and energy value 3337.0–3498.0 cal/g.Conclusions. It was revealed that samples of various genotypes of B. orientalis are a valuable source of nutrients at the flowering stage. The biochemical composition of plants depended on the genotype and stage of growth. Results of the morphometric study showed variability of investigated parameters. The obtained data can be used to predict and evaluate the results of introduction and breeding studies with B. orientalis genotypes as promising crops in Ukraine. | Мета. Визначити деякі морфометричні та біохімічні параметри генотипів Bunias orientalis L. у Національному ботанічному саду імені М. М. Гришка НАН України (НБС).Методи. Рослинну сировину B. orientalis досліджували в період квітування (6 генотипів власної селекції НБС). Визначення сухої речовини, золи, кальцію проводили згідно з Грицаєнко та ін. (2003), фосфор – згідно з Починком (1976), цукри, аскорбінову кислоту та ліпіди – згідно з Крищенком (1983), b-каротин – згідно з Плєш­ковим (1985). Енергетична цінність визначалась на кало­риметрі IKA C-200. Дані проаналізовано статистично.Результати. Висота рослин становила від 140,9 (генотип 1) до 157,5 (генотип 5) см, діаметр стебла – від 11,67 (генотип 1) до 16,1 (генотип 6) мм, кількість міжвузлів – від 18,7 (генотип 1) до 25,7 (генотип 6) шт., кількість листків на стеблі – від 14,11 (генотип 1) до 21,8 (генотип 5) шт.,  довжина листкової пластинки – від 14,2 (генотип 1) до 23,45 (генотип 6) см, ширина листкової пластинки – від 6,34 (генотип 1) до 14,5 (генотип 4) см, довжина суцвіття – від 27,4 (генотип 1) до 45,4 (генотип 3) см, ширина суцвіття – від 2,32 (генотип 1) до 4,92 (генотип 3) см та кількість стебел – від 2,55 (генотип 2) до 5,33 (генотип 1) шт. Дослідження поживних речовин у період квітування показало, що вміст сухої речовини становив 13,58–16,00%, цукрів – 5,07–8,86%, титрована кислотність – 3,28–4,25%, ліпідів – 3,33–6,61%, аскорбінової кислоти – 382,83–693,82 мг%, b-каротину – 0,94–3,48 мг%, золи – 6,79–9,2%, кальцію – 1,00–2,44%, фосфору – 1,61–2,67%, енергетична цінність – 3337,0–3498,0 кал/г.Висновки. Рослинна сировина генотипів B. orientalis – цінне джерело поживних речовин у період квітування. Біохімічний склад рослин залежить від генотипу та фази розвитку. У результаті морфометричних вимірювань показано варіабельність досліджуваних параметрів. Отримані да­ні можуть бути використані  для прогнозування та оці­нювання результатів інтродукційної і селекційної роботи з генотипами B. orientalis як перспективних культур в Україні.

Purpose. To reveal the features of agrobiological parameters formation of sweet sorghum various varieties and hybrids in the conditions of the Right-Bank Forest-Steppe of Ukraine. Methods. During 2018–2020 twenty-one varieties and hybrids of sweet sorghum of various ecological and geographical origins (Ukraine, Russia, USA, France, Germany, Hungary, Brazil) were studied in the field. Parameters like plant height and indices of their individual productivity (grain weight per panicle, 1000 grain weight, etc.), yield of dry biomass and grain, content of sugar in juice and protein in grain, as well as estimated sugar and protein yield in a crop. The counts were carried out in the phase of physiological ripeness of the culture. Results. In the group of Ukrainian varieties and hybrids, the plants were from 272 to 306 cm high, in the foreign group – from 274 to 412 cm. Varieties ‘Red Amber’, ‘Sioux’, ‘Affas CJ 899’, ‘Freed’ and ‘Early Orange’ are of high value for breeding practice, their plants were the tallest – from 388 to 412 cm. The panicle length of sweet sorghum cultivars of Ukrainian breeding ranged from 16.0 to 17.3 cm, foreign – from 11.0 to 19.4 cm. Grain weight from one panicle varied from 32.8 to 41.6 g and from 29.2 to 43.5 g, respectively. In a wide range, depending on the varietal characteristics, the indicator of the number of grains per panicle also varied from 1338 to 1708 pcs. The mass of 1000 grains of sweet sorghum ranged from 28.0 to 31.0 g in varieties and hybrids of Ukrainian breeding, in foreign ones – from 19.3 to 31.0 g. The yield of dry vegetative mass of cultivars of Ukrainian breeding was at the level of 8.24–9.11 t/ha. The highest rates were shown in hybrid ‘Mamont’ and ‘Huliver’ variety – 9.05 and 9.11 t/ha, respectively. For cultivars and hybrids of foreign breeding, this indicator varied from 7.00 to 12.17 t/ha. Significantly higher biomass in comparison with the standard variety (‘Sylosne 42’) was produced by ‘Vorai Sumac’, ‘Sorgo Cucre’, ‘Sioux’, ‘Freed’, ‘Red Amber’, ‘Mohavk’, ‘Affas CJ 899’, ‘Early Orange’ – 9.03–12.17 t/ha. The sugar content in sweet sorghum juice varied from 15.2 to 17.2%. The estimated sugar yield in Ukrainian cultivars was at the level of 0.82–0.89 t/ha, in foreign ones – from 0.72 to 1.18 t/ha. In all studied varieties it was the highest in ‘Sorgo Cucre’, ‘Sioux’, ‘Freed’, ‘Red Amber’, ‘Mohavk’, ‘Affas CJ 899’, ‘Early Orange’ – 0.94–1.18 t/ha. Conclusions. The productivity of sweet sorghum varies greatly depending on the origin of the variety and hybrid. In the conditions of the Right-Bank Forest-Steppe, in order to obtain a high sugar yield, it is advisable to grow ‘Sylosne 42’, ‘Favoryt’, ‘Troistyi’, ‘Dovista’, ‘Huliver’ varieties and ‘Ananas’, ‘Medovyi’, ‘Mamont’ hybrids. Varieties ‘Vaconia Orange’, ‘Vorai Sumac’, ‘Sorgo Cucre’ and hybrids ‘Ald Sorghum’, ‘Sioux’, ‘Freed’, ‘Red Amber’, ‘Mohavk’, ‘Affas CJ 899’, ‘Early Orange’ provide high yields of vegetative masses. Hybrids ‘Freed’, ‘Affas CJ 899’ and ‘Early Orange’ produce a large vegetative mass (11.08–12.17 t/ha), grain yield (8.00–8.15 t/ha) and a high protein content (9.8–11.3%).

Purpose. To reveal the features of agrobiological parameters formation of sweet sorghum various varieties and hybrids in the conditions of the Right-Bank Forest-Steppe of Ukraine. Methods. During 2018–2020 twenty-one varieties and hybrids of sweet sorghum of various ecological and geographical origins (Ukraine, Russia, USA, France, Germany, Hungary, Brazil) were studied in the field. Parameters like plant height and indices of their individual productivity (grain weight per panicle, 1000 grain weight, etc.), yield of dry biomass and grain, content of sugar in juice and protein in grain, as well as estimated sugar and protein yield in a crop. The counts were carried out in the phase of physiological ripeness of the culture. Results. In the group of Ukrainian varieties and hybrids, the plants were from 272 to 306 cm high, in the foreign group – from 274 to 412 cm. Varieties ‘Red Amber’, ‘Sioux’, ‘Affas CJ 899’, ‘Freed’ and ‘Early Orange’ are of high value for breeding practice, their plants were the tallest – from 388 to 412 cm. The panicle length of sweet sorghum cultivars of Ukrainian breeding ranged from 16.0 to 17.3 cm, foreign – from 11.0 to 19.4 cm. Grain weight from one panicle varied from 32.8 to 41.6 g and from 29.2 to 43.5 g, respectively. In a wide range, depending on the varietal characteristics, the indicator of the number of grains per panicle also varied from 1338 to 1708 pcs. The mass of 1000 grains of sweet sorghum ranged from 28.0 to 31.0 g in varieties and hybrids of Ukrainian breeding, in foreign ones – from 19.3 to 31.0 g. The yield of dry vegetative mass of cultivars of Ukrainian breeding was at the level of 8.24–9.11 t/ha. The highest rates were shown in hybrid ‘Mamont’ and ‘Huliver’ variety – 9.05 and 9.11 t/ha, respectively. For cultivars and hybrids of foreign breeding, this indicator varied from 7.00 to 12.17 t/ha. Significantly higher biomass in comparison with the standard variety (‘Sylosne 42’) was produced by ‘Vorai Sumac’, ‘Sorgo Cucre’, ‘Sioux’, ‘Freed’, ‘Red Amber’, ‘Mohavk’, ‘Affas CJ 899’, ‘Early Orange’ – 9.03–12.17 t/ha. The sugar content in sweet sorghum juice varied from 15.2 to 17.2%. The estimated sugar yield in Ukrainian cultivars was at the level of 0.82–0.89 t/ha, in foreign ones – from 0.72 to 1.18 t/ha. In all studied varieties it was the highest in ‘Sorgo Cucre’, ‘Sioux’, ‘Freed’, ‘Red Amber’, ‘Mohavk’, ‘Affas CJ 899’, ‘Early Orange’ – 0.94–1.18 t/ha. Conclusions. The productivity of sweet sorghum varies greatly depending on the origin of the variety and hybrid. In the conditions of the Right-Bank Forest-Steppe, in order to obtain a high sugar yield, it is advisable to grow ‘Sylosne 42’, ‘Favoryt’, ‘Troistyi’, ‘Dovista’, ‘Huliver’ varieties and ‘Ananas’, ‘Medovyi’, ‘Mamont’ hybrids. Varieties ‘Vaconia Orange’, ‘Vorai Sumac’, ‘Sorgo Cucre’ and hybrids ‘Ald Sorghum’, ‘Sioux’, ‘Freed’, ‘Red Amber’, ‘Mohavk’, ‘Affas CJ 899’, ‘Early Orange’ provide high yields of vegetative masses. Hybrids ‘Freed’, ‘Affas CJ 899’ and ‘Early Orange’ produce a large vegetative mass (11.08–12.17 t/ha), grain yield (8.00–8.15 t/ha) and a high protein content (9.8–11.3%).

Мета. З’ясувати посухостійкість рослин сортів англійських троянд. Методи. Польовий, анатомічний, біометричний. Посухостійкість рослин у польових умовах оцінювали за 6-баловою шкалою С. С. П’ятницького (1961). Kількість продихів на одиницю площі листкової пластинки визначали за допомогою сканувального електронного мікроскопа JSM-6700F (JEOL, Токіо, Японія). Результати. Посухостійкість рослин сортів англійських троянд досліджували як у польових, так і лабораторних умовах. За візуальними спостереженнями в періоди з низьким рівнем вологозабезпечення тургор листків не знижувався, тож польову посухостійкість усіх сортів оцінено у 5 балів. За параметрами щільності продихів на одиницю площі листка виділено сорти, які мають максимальні та мінімальні значення цього показника. Висновки. Усі досліджені сорти англійських троянд із колекції Державного дендрологічного парку «Олександрія» НАН України є достатньо посухостійкими в польових умовах. Однак, вони суттєво різняться за показниками щільності продихів на одиницю площі листка рослин. Найбільшу середню кількість продихів на 0,5 мм2 (163,67±7,93 шт.) зафіксовано в рослин сорту ‘Alan Titchmarsh’, найменшу (47,67±1,94 шт.) – у ‘Charles Austin’. Порівняння даних щодо морфології листків англійських троянд та щільності продихів показало, що сортам з меншими розмірами листків (‘Cottage Rose’, ‘Fisherman Friend’, ‘Noble Antony’, ‘Crocus Rose’) притаманна більша кількість продихів на одиницю площі, що вказує на їхню вищу посухостійкість. Отримані дані польових і лабораторних досліджень будуть ураховані під час формування рекомендацій щодо використання троянд для озеленення міських територій, де рослини можуть опинитися в екстремальніших, аніж у дендропарку, умовах.

The fungi of the order Onygenales can cause important human infections; however, their taxonomy and worldwide occurrence is still little known. We have studied and identified a representative number of clinical fungi belonging to that order from a reference laboratory in the USA. A total of 22 strains isolated from respiratory tract (40%) and human skin and nails (27.2%) showed a malbranchea-like morphology. Six genera were phenotypically and molecularly identified, i.e. Auxarthron/Malbranchea (68.2%), Arachnomyces (9.1%), Spiromastigoides (9.1%), and Currahmyces (4.5%), and two newly proposed genera (4.5% each). Based on the results of the phylogenetic study, we synonymized Auxarthron with Malbranchea, and erected two new genera: Pseudoarthropsis and Pseudomalbranchea. New species proposed are: Arachnomyces bostrychodes, A. graciliformis, Currahmyces sparsispora, Malbranchea gymnoascoides, M. multiseptata, M. stricta, Pseudoarthropsis crassispora, Pseudomalbranchea gemmata, and Spiromastigoides geomycoides, along with a new combination for Malbranchea gypsea. The echinocandins showed the highest in vitro antifungal activity against the studied isolates, followed by terbinafine and posaconazole; in contrast, amphotericin B, fluconazole, itraconazole and 5-fluorocytosine were less active or lacked in vitro activity against these fungi.