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Apomixis and the problem of obtaining haploids and homozygote diploids in pear (Pyrus communis L.) Texte intégral
2013
Долматов, Є. О | Джафарова, В. Є
Apomixis and the problem of obtaining haploids and homozygote diploids in pear (Pyrus communis L.) Texte intégral
2013
Долматов, Є. О | Джафарова, В. Є
The article highlights results of research over simulative apomixes in pear and its utilization for obtaining haploids and homozygote diploids. It has been established that over 50% pear varieties with failed remote hybridization are capable of generating seeds of apomictic origin producing diploid plants. Genotypes displaying maximal inclination to apomixes have been singled out. Apomictic pear seedlings obtained from foreign pollination within the limits of the same combination are inherent in profound morphological diversity. Fruit-bearing apomicts originated from one and the same maternal plant differ to the same extent as hybrid seedlings of the same family. Genetic markers have enabled to establish that these are embryo sacs in which meiosis has completed that give rise to apomictic seeds. In vitro method as used for the purpose of increasing apomictic plants output has been illustrated. The greatest induction of apomictic shoots in vitro has been reached by alternation of BAP cytokinin at concentration of 1mg/l and 2 mg/l on the background of GA3 amounting to 1,5 mg/l. Grafting with shoots in vitro on non-sterile rootstocks of pear (Pyrus communis) has increased the output of plants up to 80%. A cytological assessment of 9 apomictic samples is provided. The cytological analysis of samples of apomictic forms has certified the presence of simulative haploid parthenogenesis in pear.
Afficher plus [+] Moins [-]Apomixis and the problem of obtaining haploids and homozygote diploids in pear (Pyrus communis L.) Texte intégral
2013
Є. О. Долматов | В. Є. Джафарова
The article highlights results of research over simulative apomixes in pear and its utilization for obtaining haploids and homozygote diploids. It has been established that over 50% pear varieties with failed remote hybridization are capable of generating seeds of apomictic origin producing diploid plants. Genotypes displaying maximal inclination to apomixes have been singled out. Apomictic pear seedlings obtained from foreign pollination within the limits of the same combination are inherent in profound morphological diversity. Fruit-bearing apomicts originated from one and the same maternal plant differ to the same extent as hybrid seedlings of the same family. Genetic markers have enabled to establish that these are embryo sacs in which meiosis has completed that give rise to apomictic seeds. In vitro method as used for the purpose of increasing apomictic plants output has been illustrated. The greatest induction of apomictic shoots in vitro has been reached by alternation of BAP cytokinin at concentration of 1mg/l and 2 mg/l on the background of GA3 amounting to 1,5 mg/l. Grafting with shoots in vitro on non-sterile rootstocks of pear (Pyrus communis) has increased the output of plants up to 80%. A cytological assessment of 9 apomictic samples is provided. The cytological analysis of samples of apomictic forms has certified the presence of simulative haploid parthenogenesis in pear.
Afficher plus [+] Moins [-]Quality and marketable characteristics of highbush blueberry (Vaccinium corym- bosum L.) under the Kyiv region conditions Texte intégral
2013
Сіленко, В. О | Марченко, С. В
Quality and marketable characteristics of highbush blueberry (Vaccinium corym- bosum L.) under the Kyiv region conditions Texte intégral
2013
Сіленко, В. О | Марченко, С. В
In the article the authors present the results of the study biometric and qualitative characteristics of berries highbush blueberry varieties (Vaccinium corymbosum L.) in a specific soil and climatic conditions Forest-steppe of Ukraine (Kiev). Investigations were carried out on 11 varieties of American highbush blueberry. Phenological observations, biometric surveys, tasting score and biochemical analysis of the berries have been conducted during the growing season. By results of researches the group of early-ripening varieties includes Bluestar, Earliblue, Patriot, the middle-ripening – Jonne, Atlantis, Bluegold, Bluecrop, Chyk and late-ripening varieties – Amanda, Darrow, Toro. Duration of collecting period of ripe berries was lower (16 days) in varieties Bluestar, Earliblue, Jonne and Darrow. Berries were large sizes in the varieties of Darrow, Amanda, Jonne, in other varieties berries were medium size. By tasting score the berry of varieties Toro, Earliblue, Jonne and Darrow were the best. For biochemical parameters the better varieties were Amanda, Jonne and Earliblue.
Afficher plus [+] Moins [-]Quality and marketable characteristics of highbush blueberry (Vaccinium corym- bosum L.) under the Kyiv region conditions Texte intégral
2013
В. О. Сіленко | С. В. Марченко
In the article the authors present the results of the study biometric and qualitative characteristics of berries highbush blueberry varieties (Vaccinium corymbosum L.) in a specific soil and climatic conditions Forest-steppe of Ukraine (Kiev). Investigations were carried out on 11 varieties of American highbush blueberry. Phenological observations, biometric surveys, tasting score and biochemical analysis of the berries have been conducted during the growing season. By results of researches the group of early-ripening varieties includes Bluestar, Earliblue, Patriot, the middle-ripening – Jonne, Atlantis, Bluegold, Bluecrop, Chyk and late-ripening varieties – Amanda, Darrow, Toro. Duration of collecting period of ripe berries was lower (16 days) in varieties Bluestar, Earliblue, Jonne and Darrow. Berries were large sizes in the varieties of Darrow, Amanda, Jonne, in other varieties berries were medium size. By tasting score the berry of varieties Toro, Earliblue, Jonne and Darrow were the best. For biochemical parameters the better varieties were Amanda, Jonne and Earliblue.
Afficher plus [+] Moins [-]Gnome 2 as a donor for winter rye (Secale cereale L.) short stem Texte intégral
2013
Скорик, В. В
Gnome 2 as a donor for winter rye (Secale cereale L.) short stem Texte intégral
2013
Скорик, В. В
A stabilizing (directed) selection has created a donor of short stem for winter rye (Secale cereale L.), plant height of which ranged from 50 to 60 cm. The plant height kept symmetry of its distribution curve and the frequency accumulation in central classes (positive excess). For the first time a symbolic designation to new short-stem related Hl-2Hl-2 allele and the donor name (Gnome 2) were proposed. 28 years of stabilizing selection showed that 57% of overall genetic variability of plant height resulted from adaptive genes available for directed selection by phenotype, and 43% from dominant and epistatic factors that predetermines the expression heterosis effect. Gnome 2 donor proved to have genetic additive correlation between the pants height and number of flows per ear, ear length, weight of seeds per plant , 100 seeds weight per plant; to have reverse correlation with ear density seeds weight per ear. The height of original parent components have displayed direct additive correlation with number of flowers per ear and reverse with the ear density. The additive correlation component directly exposes «genuine» impact of parental plants on the expression of the characteristics indicated among the offspring Productive bushing of parental plants, seed weight per plant directly, and seed size (100 seeds weight) indirectly, respectively, influence the height of offspring pants. The reverse additive correlation between the parents height and 100 seeds weight in the offspring is caused by pleiotropic effect of the genes impact thus enabling to combine the desirable characteristics in one genotype. Productive bushing is by 54% due to the impact of general genetic factors among the above, in particular, 30% due to that additive, 24 due to non-additive factors. The concept of genetic improvements for productive bushing of the Gnome 2 rye implies utilization of additive effect through the directed selection, as well as application of breeding techniques for controlling the effect of heterosis caused by the genes of dominant and epistatic impact. The selection paradigm requires simultaneous genotypes selection with immediate examination of the selection results by offspring while in parallel to develop inbred lines, combining these afterwards evaluating general and specific combining ability by productive bushing. It is also to be noted that the productive bushing essentially depends on the environmental conditions, which significantly corrects the implementation of productivity potent, thereby the issue of agronomical conditions aimed at extending the expression of characteristic in question remains.
Afficher plus [+] Moins [-]Gnome 2 as a donor for winter rye (Secale cereale L.) short stem Texte intégral
2013
В. В. Скорик
A stabilizing (directed) selection has created a donor of short stem for winter rye (Secale cereale L.), plant height of which ranged from 50 to 60 cm. The plant height kept symmetry of its distribution curve and the frequency accumulation in central classes (positive excess). For the first time a symbolic designation to new short-stem related Hl-2Hl-2 allele and the donor name (Gnome 2) were proposed. 28 years of stabilizing selection showed that 57% of overall genetic variability of plant height resulted from adaptive genes available for directed selection by phenotype, and 43% from dominant and epistatic factors that predetermines the expression heterosis effect. Gnome 2 donor proved to have genetic additive correlation between the pants height and number of flows per ear, ear length, weight of seeds per plant , 100 seeds weight per plant; to have reverse correlation with ear density seeds weight per ear. The height of original parent components have displayed direct additive correlation with number of flowers per ear and reverse with the ear density. The additive correlation component directly exposes «genuine» impact of parental plants on the expression of the characteristics indicated among the offspring Productive bushing of parental plants, seed weight per plant directly, and seed size (100 seeds weight) indirectly, respectively, influence the height of offspring pants. The reverse additive correlation between the parents height and 100 seeds weight in the offspring is caused by pleiotropic effect of the genes impact thus enabling to combine the desirable characteristics in one genotype. Productive bushing is by 54% due to the impact of general genetic factors among the above, in particular, 30% due to that additive, 24 due to non-additive factors. The concept of genetic improvements for productive bushing of the Gnome 2 rye implies utilization of additive effect through the directed selection, as well as application of breeding techniques for controlling the effect of heterosis caused by the genes of dominant and epistatic impact. The selection paradigm requires simultaneous genotypes selection with immediate examination of the selection results by offspring while in parallel to develop inbred lines, combining these afterwards evaluating general and specific combining ability by productive bushing. It is also to be noted that the productive bushing essentially depends on the environmental conditions, which significantly corrects the implementation of productivity potent, thereby the issue of agronomical conditions aimed at extending the expression of characteristic in question remains.
Afficher plus [+] Moins [-]New varieties of annual aster (Callistephus chinensis (L.) Nees) by Ukrainian breeding Texte intégral
2013
Шевель, Л. О
New varieties of annual aster (Callistephus chinensis (L.) Nees) by Ukrainian breeding Texte intégral
2013
Шевель, Л. О
The article highlights the state of breeding and variety studying for Callistephus chinensis in Ukraine and, in particular, in the Horticulture Institute of NAA, and offers the summary of studying the growth and development aspects for Callistephus, various varieties productivity level, their response to specific set of weather and climatic conditions. The research objectives and methods for their achieving are disclosed, as well the requirements a variety shall comply with, namely: reaching homozygote state, distinctness and ornamentality, fusariose and weather conditions resistance. 11 descriptions for new varieties of annual aster (Anastasia, Angelina, Litnia Nich, Lybid, Oksamyt, Samanta, Sofia, Snizhana, Flamingo , Shokoladka, Tsarivna) as bred by the Horticulture Institute of NAAS were provided, namely by identification and economic and biologic characteristics, these varieties are listed in the State Register of Plant Varieties Suitable for Dissemination in Ukraine.
Afficher plus [+] Moins [-]Addressing the diversity of Xylodon raduloides complex through integrative taxonomy Texte intégral
2019
Javier Fernández-López | M. Teresa Telleria | Margarita Dueñas | Andrew W. Wilson | Mahajabeen Padamsee | Peter K. Buchanan | Gregory M. Mueller | María P. Martín
Abstract In this study, the taxonomic diversity of the Xylodon raduloides species complex (Hymenochaetales, Basidiomycota) is examined. Specimens were studied using an integrative taxonomic approach that includes molecular phylogenetic and morphological analyses, and environmental niche comparisons. Four different species were found inside the Xylodon raduloides complex, with a biogeographic distribution pattern bound by geographic regions: Europe, North America, Patagonia, and Australia–New Zealand. Molecular, morphological, and environmental evidences delimit two lineages within this complex: a Northern Hemisphere clade with longer basidiospores and wider ranges in temperature and precipitation tolerance, and a Southern Hemisphere clade with smaller and more spherical basidiospores, and an isothermal and more humid climate preference. The integrative taxonomic approach used in this study demonstrates congruence between data sets and shows how morphological and environmental characteristics contribute to the differentiation of fungal species complexes. By combining various sources of taxonomic information, three new species are described: Xylodon laurentianus, X. novozelandicus, and X. patagonicus.
Afficher plus [+] Moins [-]Erecting the system of vertical finical equalization in Ukraine Texte intégral
2013
Козенко, А. О | Дроща, П. В | Данюк, Т. А
Erecting the system of vertical finical equalization in Ukraine Texte intégral
2013
Козенко, А. О | Дроща, П. В | Данюк, Т. А
The article analyzes range of problems related to the erection and development of vertical financial equalizing on the background of processes that have triggered after Ukraine acquired its sovereignty. The basic stages and trends of the development are detected for the vertical financial equalizing in Ukraine, which essentially corresponded to the changes in arrangement of inter-budget relations. It is proved that the currently available trends in the development of inter-budget relations in Ukraine have been associated with the stages of vertical financial equalizing in Ukraine. The current system for financial vertical equalization in Ukraine is self-contradictory and slows down social and economical development of the country. Taking this into account the proposals were established as regards the main approaches to further reforming of the system of vertical financial equalization in Ukraine in terms of its shifting to the market economy, setting budget laws of the country in conformity to the legislation on the structure of executive power and local governments, as well as other acts of law determining their functions.
Afficher plus [+] Moins [-]Erecting the system of vertical finical equalization in Ukraine Texte intégral
2013
А. О. Козенко | П. В. Дроща | Т. А. Данюк
The article analyzes range of problems related to the erection and development of vertical financial equalizing on the background of processes that have triggered after Ukraine acquired its sovereignty. The basic stages and trends of the development are detected for the vertical financial equalizing in Ukraine, which essentially corresponded to the changes in arrangement of inter-budget relations. It is proved that the currently available trends in the development of inter-budget relations in Ukraine have been associated with the stages of vertical financial equalizing in Ukraine. The current system for financial vertical equalization in Ukraine is self-contradictory and slows down social and economical development of the country. Taking this into account the proposals were established as regards the main approaches to further reforming of the system of vertical financial equalization in Ukraine in terms of its shifting to the market economy, setting budget laws of the country in conformity to the legislation on the structure of executive power and local governments, as well as other acts of law determining their functions.
Afficher plus [+] Moins [-]Suitability for processing of carrot (Daucus carota L.) roots of different varieties Texte intégral
2013
Завадська, О. В | Бобось, І. М | Дяденко, Т. В
Suitability for processing of carrot (Daucus carota L.) roots of different varieties Texte intégral
2013
Завадська, О. В | Бобось, І. М | Дяденко, Т. В
The article presents summary of studying the efficiency of dry carrot production when growing in the conditions of Ukraine’s Forest-Steppe subject to a variety. A complex assessment has been completed for fresh and dried products of 10 different varieties and hybrids of carrots on the content of basic biochemical, economic and biological indicators in order to highlight the best suited for drying. Varieties have been singled out, which roots display the highest yield, best marketability, accumulate the most of dry matter, sugars, etc. A close direct correlation between the weight of roots and their marketability has been established. An evaluation of the tested roots for complex organoleptic characteristics has been completed. It is proved that carrot drying is profitable. The yield and dry matter content in roots are key influence factors on profitability of dry carrot production. By the set of parameters under studying varieties most suitable for drying, Krasniy Velikan and Dlinnaia Krasnaia, have been selected. Profitability of their drying exceeds 40%.
Afficher plus [+] Moins [-]Suitability for processing of carrot (Daucus carota L.) roots of different varieties Texte intégral
2013
О. В. Завадська | І. М. Бобось | Т. В. Дяденко
The article presents summary of studying the efficiency of dry carrot production when growing in the conditions of Ukraine’s Forest-Steppe subject to a variety. A complex assessment has been completed for fresh and dried products of 10 different varieties and hybrids of carrots on the content of basic biochemical, economic and biological indicators in order to highlight the best suited for drying. Varieties have been singled out, which roots display the highest yield, best marketability, accumulate the most of dry matter, sugars, etc. A close direct correlation between the weight of roots and their marketability has been established. An evaluation of the tested roots for complex organoleptic characteristics has been completed. It is proved that carrot drying is profitable. The yield and dry matter content in roots are key influence factors on profitability of dry carrot production. By the set of parameters under studying varieties most suitable for drying, Krasniy Velikan and Dlinnaia Krasnaia, have been selected. Profitability of their drying exceeds 40%.
Afficher plus [+] Moins [-]Combining ability of tetraploid pollinator lines of sugar beet (Beta vulgaris L. ssp. vulgaris var. altissima Doell) by the elements of productivity Texte intégral
2013
Корнєєва, М. О | Чемерис, Л. М | Мацук, М. Б
Combining ability of tetraploid pollinator lines of sugar beet (Beta vulgaris L. ssp. vulgaris var. altissima Doell) by the elements of productivity Texte intégral
2013
Корнєєва, М. О | Чемерис, Л. М | Мацук, М. Б
Evaluation of the genetic component values considered to be the important part of the selection process on creating hybrids of sugar beet and The aim of the study is to determine tetraploid pollinators combining ability Bilotserkovskoy breeding on yield and sugar content and the genetic determination of the productivity elements and their phenotypic manifestations in first generation hybrids of sugar beet. The methods of test crossing on the type top cross of pollen sterile lines of Uuladivsky and Ivanovsky origin and stabilized ployidnistyu tetraploid pollinators of beet sugar Belotserkovskoy selection on yielding of sugar beet tetraploid pollinators of Bilotserkovskoy breeding have been applied. A key role belongs to non additive gene effects in gene structure of characteristics variability yielding has been determined.The part of additive gene action pollinators is predominant in genetic control of top cross hybrids sugar content. The 16 hybrid combinations are differentiated by their parental components combining ability. Pollinators of 1007 (the yield) and 1013 (for the sugar content) have been characterized by their valuable additive gene complexes. Perspective hybrid combinations for their further breeding study are revealed, genetic determination of productivity elements and sugar yield is defined Analysis of gene interactions in sugar beet hybrids confirms the theory of genetic balance MV Turbine
Afficher plus [+] Moins [-]A revision of malbranchea-like fungi from clinical specimens in the United States of America reveals unexpected novelty Texte intégral
2021
Ernesto Rodríguez-Andrade | José F. Cano-Lira | Nathan Wiederhold | Alba Pérez-Cantero | Josep Guarro | Alberto M. Stchigel
ABSTRACT The fungi of the order Onygenales can cause important human infections; however, their taxonomy and worldwide occurrence is still little known. We have studied and identified a representative number of clinical fungi belonging to that order from a reference laboratory in the USA. A total of 22 strains isolated from respiratory tract (40%) and human skin and nails (27.2%) showed a malbranchea-like morphology. Six genera were phenotypically and molecularly identified, i.e. Auxarthron/Malbranchea (68.2%), Arachnomyces (9.1%), Spiromastigoides (9.1%), and Currahmyces (4.5%), and two newly proposed genera (4.5% each). Based on the results of the phylogenetic study, we synonymized Auxarthron with Malbranchea, and erected two new genera: Pseudoarthropsis and Pseudomalbranchea. New species proposed are: Arachnomyces bostrychodes, A. graciliformis, Currahmyces sparsispora, Malbranchea gymnoascoides, M. multiseptata, M. stricta, Pseudoarthropsis crassispora, Pseudomalbranchea gemmata, and Spiromastigoides geomycoides, along with a new combination for Malbranchea gypsea. The echinocandins showed the highest in vitro antifungal activity against the studied isolates, followed by terbinafine and posaconazole; in contrast, amphotericin B, fluconazole, itraconazole and 5-fluorocytosine were less active or lacked in vitro activity against these fungi.
Afficher plus [+] Moins [-]Researching Exotic Forms Lactuca sativa L. of Oaklieaf group Texte intégral
2013
Лещук, Н. В
Researching Exotic Forms Lactuca sativa L. of Oaklieaf group Texte intégral
2013
Лещук, Н. В
The article reveals developments of national resources of cutting lettuce of Lactuca sativa var. secalina L. varieties and methods for new varieties development involving breeding forms of Oakleaf group. Exotic forms of common knowledge varieties of Oakleaf group of salad were researched, newly developed Dublianskiy variety was applied for the official research and technical examination of plant varieties with the view of the variety official listing, as well as the rights thereof. Description of morphological description of characteristics was applied that would enable identifying new variety during the examination for distinctness, uniformity and stability. Summary of the studying value for cultivation and use of Dublianskiy variety (yield, maturity group, taste properties, tasting score, disease resistance) which is provided, which establishes working algorithm for cutting lettuce variety in various ecological gradients of its cultivation.
Afficher plus [+] Moins [-]Researching Exotic Forms Lactuca sativa L. of Oaklieaf group Texte intégral
2013
Н. В. Лещук
The article reveals developments of national resources of cutting lettuce of Lactuca sativa var. secalina L. varieties and methods for new varieties development involving breeding forms of Oakleaf group. Exotic forms of common knowledge varieties of Oakleaf group of salad were researched, newly developed Dublianskiy variety was applied for the official research and technical examination of plant varieties with the view of the variety official listing, as well as the rights thereof. Description of morphological description of characteristics was applied that would enable identifying new variety during the examination for distinctness, uniformity and stability. Summary of the studying value for cultivation and use of Dublianskiy variety (yield, maturity group, taste properties, tasting score, disease resistance) which is provided, which establishes working algorithm for cutting lettuce variety in various ecological gradients of its cultivation.
Afficher plus [+] Moins [-]Inheritance nature for the trait of resistance to neck rot in hybrid combinations of common onion under the conditions of Nosivka Variety Texting Station Texte intégral
2013
Горган, Н. О
Inheritance nature for the trait of resistance to neck rot in hybrid combinations of common onion under the conditions of Nosivka Variety Texting Station Texte intégral
2013
Горган, Н. О
The article highlights the results of studying hybrid combinations of common onion to the Botrytis allii Munn pathogen. It is found out that as for the combinations being studied the resistance to pathogen was inherited as dominant, intermediate or recessive trait. Better resistance to neck rot was inherent in the hybrid combinations which parents comprised resistant and immune varieties, insofar as the determination of inheritance coefficient has indicated F1 hybrids have this characteristic expression dependant by 50 to 78% on the parental plant (h2 =0,50–0,78). Studying the determination of the impact of both parental components on inheritance of the resistance to B. allii pathogen showed that the regression coefficient subject to hybrid combination fell within R = 0,12–0,39, while the resistance coefficient within 0,24–0,78, respectively. Efficiency of selection is proved by the trait of resistance to neck rot based on the populations of Grandina х Mavka, Olina х Grandina, Grandina х Skvyrska і Skvyrska х Grandina, as their respective inheritance coefficient reached the level of 0,42 to 0,78.
Afficher plus [+] Moins [-]Vernalization requirement of winter bread wheat modern varieties (Tritikum aestivum L.) Texte intégral
2007
Н. В. Булавка | Л. М. Голик
The study of vernalization requierement of winter bread wheat 87 modem varieties from Ukraine and Russia showed significant domination - 81.6% - of varieties with short vernalization requierement (30-40 days). Vernalization requierement differences among varieties from different climatic zones were revealed.
Afficher plus [+] Moins [-]Growth and development of sugar sorghum (Sorghum saccharatum L. Pers.) plants at different terms of sowing and seeding depth in the Forest-Steppe of Ukraine Texte intégral
2013
Герасименко, Л. А
Growth and development of sugar sorghum (Sorghum saccharatum L. Pers.) plants at different terms of sowing and seeding depth in the Forest-Steppe of Ukraine Texte intégral
2013
Герасименко, Л. А
The article features the results of research on phenological observations, biometric parameters of growth and development of the plants of sugar sorghum Silosnoe 42 variety during the vegetation at different terms of sowing and seeding depth in the Forest-Steppe of Ukraine. In particular, we show data on ground germination capacity of the seeds, the duration of the interphase periods, tillering, plant height and the diameter of the stem. It was established that the planting of sugar sorghum in mid-May (the third sowing date) to the seeding depth of 4...6 cm enabled better plant development, as well as in these conditions the maximum values of plant growth and development were registered compared to the other test editions. Therefore, were would suggest the third term of sowing and seed depth 4...6 cm for growing the crop in this area.
Afficher plus [+] Moins [-]Growth and development of sugar sorghum (Sorghum saccharatum L. Pers.) plants at different terms of sowing and seeding depth in the Forest-Steppe of Ukraine Texte intégral
2013
Л. А. Герасименко
The article features the results of research on phenological observations, biometric parameters of growth and development of the plants of sugar sorghum Silosnoe 42 variety during the vegetation at different terms of sowing and seeding depth in the Forest-Steppe of Ukraine. In particular, we show data on ground germination capacity of the seeds, the duration of the interphase periods, tillering, plant height and the diameter of the stem. It was established that the planting of sugar sorghum in mid-May (the third sowing date) to the seeding depth of 4...6 cm enabled better plant development, as well as in these conditions the maximum values of plant growth and development were registered compared to the other test editions. Therefore, were would suggest the third term of sowing and seed depth 4...6 cm for growing the crop in this area.
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