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Plant DNA banks for genetic resources conservation (review) Texte intégral
2016
Волкова, Н. Е
Plant DNA banks for genetic resources conservation (review) Texte intégral
2016
Волкова, Н. Е
Purpose. Literature review of DNA banks creation as the current strategy of plant genetic resources conservation. Results. The current state of plant genetic resources conservation was analyzed in the context of the threat of genetic erosion. The importance of DNA banks was shown which function is to store DNA samples and associated products and disseminate them for research purposes. The main DNA banks in the world were described, including the Republican DNA Bank of Human, Animals, Plants and Microorganisms at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus. Stages of DNA banking were considered: tissue sampling (usually from leaves), cell destruction, DNA extraction, DNA storage. Different methods of tissue sampling, extraction and DNA storage were compared. The need for Plant DNA Bank creation in Ukraine was highlighted. Conclusions. DNA collections is an important resource in the global effort to overcome the crisis in biodiversity, for managing world genetic resources and maximizing their potential.
Afficher plus [+] Moins [-]Plant DNA banks for genetic resources conservation (review) Texte intégral
2016
Н. Е. Волкова
Purpose. Literature review of DNA banks creation as the current strategy of plant genetic resources conservation. Results. The current state of plant genetic resources conservation was analyzed in the context of the threat of genetic erosion. The importance of DNA banks was shown which function is to store DNA samples and associated products and disseminate them for research purposes. The main DNA banks in the world were described, including the Republican DNA Bank of Human, Animals, Plants and Microorganisms at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus. Stages of DNA banking were considered: tissue sampling (usually from leaves), cell destruction, DNA extraction, DNA storage. Different methods of tissue sampling, extraction and DNA storage were compared. The need for Plant DNA Bank creation in Ukraine was highlighted. Conclusions. DNA collections is an important resource in the global effort to overcome the crisis in biodiversity, for managing world genetic resources and maximizing their potential.
Afficher plus [+] Moins [-]Method for determination of varietal purity (typicality), hybridity, sterility of seed lots based on the establishment of the quantitative ratio of alleles of DNA markers Texte intégral
2016
Вдовиченко, Ж. В | Спиридонов, В. Г | Хомутовська, С. В | Парій, М. Ф
Method for determination of varietal purity (typicality), hybridity, sterility of seed lots based on the establishment of the quantitative ratio of alleles of DNA markers Texte intégral
2016
Вдовиченко, Ж. В | Спиридонов, В. Г | Хомутовська, С. В | Парій, М. Ф
Purpose. To develop a conceptually new method for determination of varietal purity (typicality), hybridity, sterility of seed lots. Methods of molecular biology (genomic DNA extraction, PCR with SSR markers application, capillary electrophoresis), genetic, statistical, mathematical analysis. Results. New method for determining the varietal qualities of seed lot was developed that consists of the following steps: simultaneous DNA extraction from a representative sample of aggregated seeds; PCR and further analysis of the amplification products by determination of the qualitative and quantitative composition of SSR-markers’ alleles; calculation of values of varietal seed lot quality using experimentally derived allele ratios. Conclusions. The developed method for determining varietal qualities of seed lots allows to reduce significantly the consumption of materials, time and labor during the analysis. Consistent qualification and quantification of alleles in the total sample of a seed lot is a conceptually new approach to establish varietal purity (typicality), hybridity, sterility.
Afficher plus [+] Moins [-]A revision of Salispina, its placement in a new family, Salispinaceae (Rhipidiales), and description of a fourth species, S. hoi sp. nov Texte intégral
2018
Reuel M. Bennett | Mark Kevin Devanadera | Gina R. Dedeles | Marco Thines
Abstract The genus Salispina was recently described for saprotrophic estuarine oomycetes with aculeolate or spiny sporangia. The genus currently contains three species, S. intermedia, S. lobata, and S. spinosa, the latter two previously included in Halophytophthora. During a survey of mangrove-inhabiting oomycetes in the Philippines, an isolate of Salispina (USTCMS 1611), was obtained from a decaying mangrove leaf. This isolate differed from other species in the genus in a unique combination of morphological and biological characters. Phylogenetic analysis revealed it to be the sister lineage of S. lobata. Consequently, the new species name S. hoi is introduced for the isolate. In addition, Salispina spp. grouped with Sapromyces of Rhipidiales with strong support, but differs from all other known genera of the order in the weak formation of hyphal constrictions, and absence of basal thalli and a holdfast network. The new family Salispinaceae is, therefore, described to accommodate Salispina in the order Rhipidiales.
Afficher plus [+] Moins [-]Effect of plant growth regulators on the yield of such red beetroot varieties as Bordeaux Kharkivskyi and Aktion Texte intégral
2015
І. М. Бобось | М. В. Ободовський
Effect of plant growth regulators on the yield of such red beetroot varieties as Bordeaux Kharkivskyi and Aktion Texte intégral
2015
І. М. Бобось | М. В. Ободовський
Purpose. Revealing of adaptive properties of plant varieties of red beet Bordeaux Kharkivskyi and Aktion based on the study of plant growth regulators for marketable production in the Steppe zone of Ukraine. Methods. Field, laboratory ones, variance analysis. Results. The influence of plant growth regulators (Lignogumat, Betastimulin) on earliness and productivity of such red beet varieties as Bordeaux Kharkivskyi and Aktion was established. Conclusions. Plant growth regulators (Betastimulin, Lignogumat) stimulated seed sprouting of red beet Bordeaux Kharkivskyi and Aktion varieties, activated vegetative plant growth increasing marketable yield. Application of Betastimulin (0.2%) for red beet Kharkivskyi Bordeaux and Aktion varieties provides the highest number of marketable beetroots, thus forming marketable yield of 44.7 and 54.9 t/ha respectively with the average beetroot weight of 328–519 g.
Afficher plus [+] Moins [-]Effect of plant growth regulators on the yield of such red beetroot varieties as Bordeaux Kharkivskyi and Aktion Texte intégral
2015
Бобось, І. М | Ободовський, М. В
Purpose. Revealing of adaptive properties of plant varieties of red beet Bordeaux Kharkivskyi and Aktion based on the study of plant growth regulators for marketable production in the Steppe zone of Ukraine. Methods. Field, laboratory ones, variance analysis. Results. The influence of plant growth regulators (Lignogumat, Betastimulin) on earliness and productivity of such red beet varieties as Bordeaux Kharkivskyi and Aktion was established. Conclusions. Plant growth regulators (Betastimulin, Lignogumat) stimulated seed sprouting of red beet Bordeaux Kharkivskyi and Aktion varieties, activated vegetative plant growth increasing marketable yield. Application of Betastimulin (0.2%) for red beet Kharkivskyi Bordeaux and Aktion varieties provides the highest number of marketable beetroots, thus forming marketable yield of 44.7 and 54.9 t/ha respectively with the average beetroot weight of 328–519 g.
Afficher plus [+] Moins [-]Influence of agroecological conditions of edaphic-climatic macro- and microzones on adaptability and productivity of new soft winter wheat varieties Texte intégral
2015
О. Л. Уліч | Н. П. Костенко | С. О. Ткачик | С. М. Гринів | М. І. Загинайло | В. М. Лисікова | Ю. Ф. Терещенко
Influence of agroecological conditions of edaphic-climatic macro- and microzones on adaptability and productivity of new soft winter wheat varieties Texte intégral
2015
О. Л. Уліч | Н. П. Костенко | С. О. Ткачик | С. М. Гринів | М. І. Загинайло | В. М. Лисікова | Ю. Ф. Терещенко
Purpose. To study the influence of agriecological conditions of edaphic-climatic macro- and microzones on adaptability and productivity of new soft winter wheat, identification and selection of complementary varieties for appropriate conditions. Methods. Field, laboratory, analytical and statistical ones. Results. Peculiarities of the formation of productivity of varieties and its stability cultivation on the condions of cultivation in edaphic-climatic zones, subzones and microzones, including favourable and unfavourable years were studied. For realizing the potential of their productivity, it is advisable to introduce a differentiated approach to their cultivation in agri-climatic zones, subzones, microzones according to the requirements of biological characteristics of varieties, their natural adaptive capacity, natural and ecological environment of these zones. Complementary varieties were identified and recommended for use in edaphic-climatic zones, subzones and microzones that demonstrated high plasticity, better adaptability to favourable agri-ecological factors and were more tolerant and resistant to adverse ones. Conclusions. According to the set of productivity and ecological plasticity indices, it is desirable to cultivate varieties Sotnytsya, Kalancha, Hurt, Tonatsiya, Dobrochyn, Gubernator Donu in the Steppe zone; Sotnytsya, Oriyka, Lira Odeska, Tatsitus and Fidelius – in the Forest-Steppe; Tatsitus, Fidelius, Sailor, Etela – in the Polissia.
Afficher plus [+] Moins [-]Influence of agroecological conditions of edaphic-climatic macro- and microzones on adaptability and productivity of new soft winter wheat varieties Texte intégral
2015
Уліч, О. Л | Kostenko, N. P. | Ткачик, С. О | Гринів, С. М | Загинайло, М. І | Лисікова, В. М | Терещенко, Ю. Ф
Purpose. To study the influence of agriecological conditions of edaphic-climatic macro- and microzones on adaptability and productivity of new soft winter wheat, identification and selection of complementary varieties for appropriate conditions. Methods. Field, laboratory, analytical and statistical ones. Results. Peculiarities of the formation of productivity of varieties and its stability cultivation on the condions of cultivation in edaphic-climatic zones, subzones and microzones, including favourable and unfavourable years were studied. For realizing the potential of their productivity, it is advisable to introduce a differentiated approach to their cultivation in agri-climatic zones, subzones, microzones according to the requirements of biological characteristics of varieties, their natural adaptive capacity, natural and ecological environment of these zones. Complementary varieties were identified and recommended for use in edaphic-climatic zones, subzones and microzones that demonstrated high plasticity, better adaptability to favourable agri-ecological factors and were more tolerant and resistant to adverse ones. Conclusions. According to the set of productivity and ecological plasticity indices, it is desirable to cultivate varieties Sotnytsya, Kalancha, Hurt, Tonatsiya, Dobrochyn, Gubernator Donu in the Steppe zone; Sotnytsya, Oriyka, Lira Odeska, Tatsitus and Fidelius – in the Forest-Steppe; Tatsitus, Fidelius, Sailor, Etela – in the Polissia.
Afficher plus [+] Moins [-]Using cluster analysis as a method of classification of the genus Salix L. representatives Texte intégral
2015
М. В. Роїк | В. В. Баликіна
Using cluster analysis as a method of classification of the genus Salix L. representatives Texte intégral
2015
М. В. Роїк | В. В. Баликіна
Purpose. To study interactions among the representatives of the genus Salix L. through the cluster analysis, form groups of closely related species and hybrid forms basing on differences of morphological parameters of leaves. Methods. Field, cluster analysis and tree graphics. Results. Willow species were grouped according to absolute parameters of leaf, and three groups of clusters were identified. The degree of affinity between species were assessed using values of an Euclidean distance. Distinctive features of leaf parameters were defined: length of a leaf blade (Ll), distance (cm) between the leaf tip and its maximum width (SDmxT) and the distance between the leaf tip (cm) and the line of its width that corresponds to the length of petiole (SLpT). Conclusions. Using the willow species collection as an example, diagnostically valuable quantitative parameters of leaves were revealed, the use of which allows to identify willow species and hybrid forms through PC applications.
Afficher plus [+] Moins [-]Using cluster analysis as a method of classification of the genus Salix L. representatives Texte intégral
2015
Роїк, М. В | Баликіна, В. В
Purpose. To study interactions among the representatives of the genus Salix L. through the cluster analysis, form groups of closely related species and hybrid forms basing on differences of morphological parameters of leaves. Methods. Field, cluster analysis and tree graphics. Results. Willow species were grouped according to absolute parameters of leaf, and three groups of clusters were identified. The degree of affinity between species were assessed using values of an Euclidean distance. Distinctive features of leaf parameters were defined: length of a leaf blade (Ll), distance (cm) between the leaf tip and its maximum width (SDmxT) and the distance between the leaf tip (cm) and the line of its width that corresponds to the length of petiole (SLpT). Conclusions. Using the willow species collection as an example, diagnostically valuable quantitative parameters of leaves were revealed, the use of which allows to identify willow species and hybrid forms through PC applications.
Afficher plus [+] Moins [-]On streamlining the Ukrainian names of plants. Information 4. Taxonomic diversity and species names for the genus Fragaria L. Texte intégral
2015
В. М. Меженський
On streamlining the Ukrainian names of plants. Information 4. Taxonomic diversity and species names for the genus Fragaria L. Texte intégral
2015
В. М. Меженський
Purpose. Reviewing a history of formation and current state of the national nomenclature and streamlining the Ukrainian scientific species names for the genus Fragaria L. Results. Controversial attitudes towards the use of names «sunytsi» (= garden strawberry) and «polunytsi» (= hill strawberry) are inherently present in the Ukrainian nomenclature of the genus Fragaria L. The Ukrainian scientific names of species of this group of plants should be brought into line with the generic name “Sunytsi” in plural. Nothogeneric name x Fragophora Mezhenskyj (= Dasiphora Rafin. x Fragaria L.) is proposed. Conclusions. In a professional environment it is necessary to use only scientific names of species of the genus Fragaria – Sunytsi (= garden strawberry) that is used in plural in Ukrainian. Concerning garden strawberry, in every day life and popular literature the usage of a derivative sunytsia (in Ukrainian) in singular as well as polunytsia, polunytsi (in Ukrainian) may be permissible.
Afficher plus [+] Moins [-]On streamlining the Ukrainian names of plants. Information 4. Taxonomic diversity and species names for the genus Fragaria L Texte intégral
2015
Меженський, В. М
Purpose. Reviewing a history of formation and current state of the national nomenclature and streamlining the Ukrainian scientific species names for the genus Fragaria L. Results. Controversial attitudes towards the use of names «sunytsi» (= garden strawberry) and «polunytsi» (= hill strawberry) are inherently present in the Ukrainian nomenclature of the genus Fragaria L. The Ukrainian scientific names of species of this group of plants should be brought into line with the generic name “Sunytsi” in plural. Nothogeneric name x Fragophora Mezhenskyj (= Dasiphora Rafin. x Fragaria L.) is proposed. Conclusions. In a professional environment it is necessary to use only scientific names of species of the genus Fragaria – Sunytsi (= garden strawberry) that is used in plural in Ukrainian. Concerning garden strawberry, in every day life and popular literature the usage of a derivative sunytsia (in Ukrainian) in singular as well as polunytsia, polunytsi (in Ukrainian) may be permissible.
Afficher plus [+] Moins [-]Ecological stability and plasticity of potato varieties in Polissia Texte intégral
2015
Е. Р. Ермантраут | З. Б. Києнко | В. М. Маційчук | О. М. Фещук
Ecological stability and plasticity of potato varieties in Polissia Texte intégral
2015
Е. Р. Ермантраут | З. Б. Києнко | В. М. Маційчук | О. М. Фещук
Purpose. Substantiating the selection of potato varieties for their ecological plasticity and stability to grow in Polissia. Methods. Field, laboratory, analitycal and statystical ones. Results. Impact of weather conditions during the growing season on the stability of potato varieties was estimated by indicators of yield, starch content and collection over the years by determining the average, standard deviation, minimum, maximum values and range of variation. ‘Memphis’, ‘Faluka’, ‘Arsenal’ and ‘Evolution’ were distinguished from the studied potato varieties by high genetic stability – their deviation from the average variance marked «minus» had values that were significantly less than zero. ‘Faktor’, ‘Flamenko’ and ‘Evora’ varieties are plastic (deviation is close to ±0), ‘Kolomba’, ‘Arizona’ and ‘Voliumiia’ ones – very plastic (deviation is most distant from zero). The studied varieties were distributed by homeostasis as follows: ‘Arizona’, ‘Colombo’, ‘Factor’ and ‘Memphis’ are the most valuable. Conclusions. ‘Evora’ potato variety can be considered intensive in terms of yield. ‘Evolution’ and ‘Flamenco’ varieties formed stable yields, during the years of study the difference between their maximum and minimum yields was the least – 8.2 and 9.3 tons per ha, respectively. Environmental assessment according to Eberhart and Russell indicates a high genetic stability of such potato varieties as ‘Arizona’, ‘Volyumiia’, ‘Kolombo’, ‘Evora’ and ‘Flamenko’. Conditions of Zhytomyr Oblast Plant Varieties Studying Centre are the most favorable among the studied regions to cultivate potato varieties.
Afficher plus [+] Moins [-]Ecological stability and plasticity of potato varieties in Polissia Texte intégral
2015
Ермантраут, Е. Р | Києнко, З. Б | Маційчук, В. М | Фещук, О. М
Purpose. Substantiating the selection of potato varieties for their ecological plasticity and stability to grow in Polissia. Methods. Field, laboratory, analitycal and statystical ones. Results. Impact of weather conditions during the growing season on the stability of potato varieties was estimated by indicators of yield, starch content and collection over the years by determining the average, standard deviation, minimum, maximum values and range of variation. ‘Memphis’, ‘Faluka’, ‘Arsenal’ and ‘Evolution’ were distinguished from the studied potato varieties by high genetic stability – their deviation from the average variance marked «minus» had values that were significantly less than zero. ‘Faktor’, ‘Flamenko’ and ‘Evora’ varieties are plastic (deviation is close to ±0), ‘Kolomba’, ‘Arizona’ and ‘Voliumiia’ ones – very plastic (deviation is most distant from zero). The studied varieties were distributed by homeostasis as follows: ‘Arizona’, ‘Colombo’, ‘Factor’ and ‘Memphis’ are the most valuable. Conclusions. ‘Evora’ potato variety can be considered intensive in terms of yield. ‘Evolution’ and ‘Flamenco’ varieties formed stable yields, during the years of study the difference between their maximum and minimum yields was the least – 8.2 and 9.3 tons per ha, respectively. Environmental assessment according to Eberhart and Russell indicates a high genetic stability of such potato varieties as ‘Arizona’, ‘Volyumiia’, ‘Kolombo’, ‘Evora’ and ‘Flamenko’. Conditions of Zhytomyr Oblast Plant Varieties Studying Centre are the most favorable among the studied regions to cultivate potato varieties.
Afficher plus [+] Moins [-]Plant varieties protection in the European Union: legal aspects Texte intégral
2015
Якубенко, Н. Б | Федяй, М. В | Линчак, Н. Б | Скоблікова, С. М
Plant varieties protection in the European Union: legal aspects Texte intégral
2015
Якубенко, Н. Б | Федяй, М. В | Линчак, Н. Б | Скоблікова, С. М
Purpose. Reviewing the EU legislation on plant varieties protection including documents of the International Union for the Protection of New Varieties of Plants (UPOV) that related to the national laws of its member states. Results. Based on the sources processed, the authors give a brief analysis of the basic EU Council Regulation № 2100/94 of 27 July 1994 on Community plant variety rights and regulations of the European Commission adopted to implement it. Basic copyright concepts, features of plant varieties protection in the EU, including those under the UPOV Convention, are also considered. Conclusions. For Ukraine, obtaining full membership in the EU, on the one hand, requires harmonization of its laws with the relevant EU legislation, on the other – improvement of the present system of plant varieties protection taking into account national interests of Ukraine.
Afficher plus [+] Moins [-]Plant varieties protection in the European Union: legal aspects Texte intégral
2015
Н. Б. Якубенко | М. В. Федяй | Н. Б. Линчак | С. М. Скоблікова
Purpose. Reviewing the EU legislation on plant varieties protection including documents of the International Union for the Protection of New Varieties of Plants (UPOV) that related to the national laws of its member states. Results. Based on the sources processed, the authors give a brief analysis of the basic EU Council Regulation № 2100/94 of 27 July 1994 on Community plant variety rights and regulations of the European Commission adopted to implement it. Basic copyright concepts, features of plant varieties protection in the EU, including those under the UPOV Convention, are also considered. Conclusions. For Ukraine, obtaining full membership in the EU, on the one hand, requires harmonization of its laws with the relevant EU legislation, on the other – improvement of the present system of plant varieties protection taking into account national interests of Ukraine.
Afficher plus [+] Moins [-]Economic efficiency and bioenergetic evaluation of marketable lettuce production Texte intégral
2015
Н. В. Лещук
Economic efficiency and bioenergetic evaluation of marketable lettuce production Texte intégral
2015
Н. В. Лещук
Purpose. Scientific substantiation of agrotechnological methods used for marketable production of lettuce varieties Lactuca sativa L. according to economic and bioenergy evaluation. Methods. Field, laboratory, analytical and statistical ones. Results. Economic assessment of elements of cultivation technology for all lettuce varieties was undertaken considering different sowing time (early spring, late spring, spring-and-summer period and before the winter season), growing techniques (direct sowing, seedling method – planting pots, seedling method – planting containers) and the use of organic fertilizer Dominanta. Calculation of economic efficiency indicators of marketable lettuce production was scientifically grounded, and bioenergetic evaluation of its growing was made. Economic feasibility of seedling method of growing as well as sowing time and delivery of marketable products to the consumer was confirmed. Conclusions. Technological elements of lettuce growing including loose-leaf, butterhead, romaine and stem subvarieties provided increase of marketable products by 13,38; 3,92; 2,07; 3,20 t/ha respectively. The level of profitability of lettuce production was within 83–141% (butterhead) 86–130% (loose-leaf), 185–214% (romaine), 131–137% (stem).
Afficher plus [+] Moins [-]Economic efficiency and bioenergetic evaluation of marketable lettuce production Texte intégral
2015
Лещук, Н. В
Purpose. Scientific substantiation of agrotechnological methods used for marketable production of lettuce varieties Lactuca sativa L. according to economic and bioenergy evaluation. Methods. Field, laboratory, analytical and statistical ones. Results. Economic assessment of elements of cultivation technology for all lettuce varieties was undertaken considering different sowing time (early spring, late spring, spring-and-summer period and before the winter season), growing techniques (direct sowing, seedling method – planting pots, seedling method – planting containers) and the use of organic fertilizer Dominanta. Calculation of economic efficiency indicators of marketable lettuce production was scientifically grounded, and bioenergetic evaluation of its growing was made. Economic feasibility of seedling method of growing as well as sowing time and delivery of marketable products to the consumer was confirmed. Conclusions. Technological elements of lettuce growing including loose-leaf, butterhead, romaine and stem subvarieties provided increase of marketable products by 13,38; 3,92; 2,07; 3,20 t/ha respectively. The level of profitability of lettuce production was within 83–141% (butterhead) 86–130% (loose-leaf), 185–214% (romaine), 131–137% (stem).
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