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Effects of microplastic particles and leaching additive on the life history and morphology of Daphnia magna
2019
Schrank, Isabella | Trotter, Benjamin | Dummert, Julia | Scholz-Böttcher, Barbara M. | Löder, Martin G.J. | Laforsch, Christian
Plastic waste is continuously introduced not only into marine, but also freshwater environments, where it fragments into microplastics. Organisms may be affected by the particles themselves due to ingestion and indirectly via incorporated additives such as plasticizers, since these substances have the ability to leach out of the polymer matrix. Although it has been indicated that the likelihood of additives leaching out into the gut lumen of organisms exposed to microplastics is low, studies distinguishing between the effects of the synthetic polymer itself and incorporated additives of the same polymer are scarce. Since this is obligatory for risk assessment, we analyzed the chronic effects of flexible polyvinylchloride (PVC), a widely used polymer, containing the plasticizer diisononylphthalate (DiNP) on morphology and life history of the freshwater crustacean Daphnia magna and compared these effects with the effects of rigid PVC, lacking DiNP, as well as a glass bead control. After up to 31 days of exposure, rigid PVC and glass beads did not affect body length and relative tail spine length of D. magna, whereas flexible PVC led to an increased body length and a reduced number of offspring. None of the treatments increased the mortality significantly. We were able to show that 2.67μg/L DiNP leached out of the flexible PVC into the surrounding medium using GC-MS. Yet, we were not able to measure leachate inside the gut lumen of D. magna. The effects emerged towards the end of the experiment, due to the time dependent process of leaching. Therefore, the results highlight the relevance of long-term chronic exposure experiments, especially as leaching of additives takes time. Further, our study shows the importance to distinguish between microplastics containing leachable additives and the raw polymer in ecotoxicological testing.
Afficher plus [+] Moins [-]Recent advances in toxicological research of nanoplastics in the environment: A review
2019
Nanoplastics have attracted increasing attention in recent years due to their widespread existence in the environment and the potential adverse effects on living organisms. In this paper, the toxic effects of nanoplastics on organisms were systematically reviewed. The translocation and absorption of nanoplastics, as well as the release of additives and contaminants adsorbed on nanoplastics in the organism body were discussed, and the potential adverse effects of nanoplastics on human health were evaluated. Nanoplastics can be ingested by organisms, be accumulated in their body and be transferred along the food chains. Nanoplastics showed effects on the growth, development and reproduction of organisms, and disturbing the normal metabolism. The toxic effects on living organisms mainly depended on the surface chemical properties and the particle size of nanoplastics. Positively charged nanoplastics showed more significant effects on the normal physiological activity of cells than negatively charged nanoplastics, and smaller particle sized nanoplastics could more easily penetrate the cell membranes, hence, accumulated in tissues and cells. Additionally, the release of additives and contaminants adsorbed on nanoplastics in organism body poses more significant threats to organisms than nanoplastics themselves. However, there are still knowledge gaps in the determination and quantification of nanoplastics, as well as their contaminant release mechanisms, degradation rates and process from large plastics to nanoplastics, and the transportation of nanoplastics along food chains. These challenges would hinder the risk assessment of nanoplastics in the environment. It is necessary to further develop the risk assessment of nanoplastics and deeply investigate its toxicological effects.
Afficher plus [+] Moins [-]A comprehensive risk assessment of human inhalation exposure to atmospheric halogenated flame retardants and organophosphate esters in an urban zone
2019
Hu, Yuan-Jie | Bao, Lian-Jun | Huang, Chun-Li | Li, Shao-Meng | Zeng, E. Y. (Eddy Y.)
Inhalation exposure to flame retardants used as additives to minimize fire risk and plasticizers is ubiquitous in human daily activities, but has not been adequately assessed. To address this research gap, the present study conducted an assessment of human health risk for four age groups through inhalation exposure to size fractionated particle-bound and gaseous halogenated flame retardants (polybrominated diphenyl ethers (PBDEs) and alternative halogenated flame retardants (AHFRs)) and organophosphate esters (OPEs) at indoor and outdoor environments (school, office, and residence) in three districts of a megacity (Guangzhou, China). Results demonstrated that OPEs were the dominant components among all targets. Indoor daily intakes of PBDEs and OPEs were 13–16 times greater than outdoor levels for all age groups. Gaseous OPEs contributed significantly greater than particle-bound compounds to daily intakes of all target compounds. Based on the different life scenarios, hazard quotient (HQ) and incremental life cancer risk (ILCR) from adults exposure to PBDEs and OPEs in indoor and outdoor settings were the greatest, followed by adolescents, children, and seniors. The estimated HQ and ILCR for all age groups both indoors and outdoors were lower than the safe level (HQ = 1 and ILCR = 10−6), indicating that the potential health risk for local residents in Guangzhou via inhalation exposure to atmospheric halogenated flame retardants and OPEs was low.
Afficher plus [+] Moins [-]Organophosphate ester and phthalate ester metabolites in urine from primiparas in Shenzhen, China: Implications for health risks
2019
Chen, Yi | Jiang, Lei | Lu, Shaoyou | Kang, Li | Luo, Xianru | Liu, Guihua | Cui, Xinyi | Yu, Yingxin
Organophosphate esters (OPEs) and phthalate esters (PAEs) are extensively used as additives in commercial and household products. However, knowledge on human exposure to OPEs and PAEs remains limited in China. This study aimed to investigate OPE and PAE metabolites in urine samples of primiparas and to evaluate the cumulative risk of OPE and PAE exposure. A total of 8 OPE metabolites and 11 PAE metabolites were measured in urine samples of 84 primiparas from Shenzhen, China. The OPE metabolites were found in at least 72% of the urine samples with bis(2-chloroethyl) phosphate (BCEP) being the dominant analogue. Among the 11 PAE metabolites, mono-n-butyl phthalate (mBP) was the most abundant analogue and had a median concentration (139 μg/L) greater than those reported in urine samples from other countries and regions. A significant, positive correlation was found between Σ₈OPEMs (the sum of 8 OPE metabolites) and body mass index (BMI). The urinary concentration of Σ₁₁PAEMs (the sum of 11 PAE metabolites) was positively associated with the time of computer using by the primiparas. The estimated daily intakes (EDI) of tris(2-chlorethyl) phosphate (TCEP, the parent chemical of BCEP) and di-n-butyl phthalate (DnBP, the parent chemical of mBP) were determined to be 0.47 and 9.14 μg/kg bw/day, respectively. The 95th percentile EDI values for TCEP and DnBP both exceeded their corresponding reference doses. Twelve and fifty-five percentage of the primiparas were estimated to have HIRfD (hazard index corresponding to reference doses) and HITDI (hazard index corresponding to tolerable daily intake) values exceeding 1 for OPEs and PAEs, respectively, suggesting a relatively high exposure risk.
Afficher plus [+] Moins [-]The release and earthworm bioaccumulation of endogenous hexabromocyclododecanes (HBCDDs) from expanded polystyrene foam microparticles
2019
Li, Bing | Lan, Zhonghui | Wang, Lei | Sun, Hongwen | Yao, Yiming | Zhang, Kai | Zhu, Lusheng
Hexabromocyclododecanes (HBCDDs) are common chemical additives in expanded polystyrene foam (EPS). To evaluate the bioaccumulation potential of endogenous HBCDDs in EPS microparticles by earthworms, two ecologically different species of earthworms (Eisenia fetida and Metaphire guillelmi) were exposed to soil added with EPS microparticles of different particle sizes (EPS2000, 830–2000 μm and EPS830, <830 μm). To clarify the accumulation mechanisms, leaching experiments using EPS microparticles in different solutions were conducted. After exposure to EPS microparticles-amended soils (S-EPS) for 28 d, the total concentrations of HBCDDs reached 307–371 ng g−1 dw in E. fetida and 90–133 ng g−1 dw in M. guillelmi, which were higher than those in earthworms exposed to the soil that was artificially contaminated with a similar level of HBCDDs directly (ACS). The accumulation of HBCDDs in earthworms was significantly influenced by EPS microparticles' size and earthworms' species. The total concentrations of HBCDDs in earthworms' cast were significantly higher than the theoretical concentration of HBCDDs in S-EPS, which suggested that EPS microparticles can be ingested by earthworms. The release rate of HBCDDs from EPS5000 (2000–5000 μm) into water-based solutions (<1%) after a 3.5-h incubation was far lower than that into earthworm digestive fluid (7%). These results illustrated that the ingestion of EPS microparticles and consequent solubilization of HBCDDs by digestive fluid play an important role in the accumulation of HBCDDs contained in EPS microparticles in earthworms. After a 28-d incubation with the soil solution, 4.9% of the HBCDDs was accumulatively leached from the EPS5000, which indicated that HBCDDs can be released from EPS microparticles to soil environment, and then accumulated by earthworms. Moreover, similar to those exposed to ACS, the diastereoisomer- and enantiomer-specific accumulation of HBCDDs in earthworms occurred when exposed to S-EPS. This study provides more evidence for the risk of microplastics to the soil ecosystem.
Afficher plus [+] Moins [-]Toxicity assessment of pollutants sorbed on environmental sample microplastics collected on beaches: Part I-adverse effects on fish cell line
2019
Pannetier, Pauline | Cachot, Jérôme | Clérandeau, Christelle | Faure, Florian | Van Arkel, Kim | de Alencastro, Luiz F. | Levasseur, Clément | Sciacca, Frédéric | Bourgeois, Jean-Pascal | Morin, Bénédicte
Microplastics (MPs), are tiny plastic fragments from 1 μm to 5 mm generally found in the aquatic environment which can be easily ingested by organisms and may cause chronic physical but also toxicological effects. Toxicological assays on fish cell lines are commonly used as an alternative tool to provide fast and reliable assessment of the toxic and ecotoxic properties of chemicals or mixtures. Rainbow trout liver cell line (RTLW-1) was used to evaluate the toxicity of pollutants sorbed to MPs sampled in sandy beaches from different islands around the world during the first Race for Water Odyssey in 2015. The collected MPs were analyzed for polymer composition and associated persistent organic pollutants: polycyclic aromatic hydrocarbons (PAHs), polychlorobiphenyls (PCBs) and dichlorodiphenyltrichloroethane (DDT). In addition, DMSO-extracts from virgin MPs, MPs artificially coated with B[a]P and environmental MPs were analyzed with different bioassays: MTT reduction assay (MTT), ethoxyresorufin-O-deethylase (EROD) assay and comet assay. Microplastics from sand beaches were dominated by polyethylene, followed by polypropylene fragments with variable proportions. Organic pollutants found on plastic from beach sampling was PAHs (2–71 ng g⁻¹). Samples from Bermuda (Somerset Long Bay) and Hawaii (Makapu'u) showed the highest concentration of PAHs and DDT respectively. No toxicity was observed for virgin microplastics. No cytotoxicity was observed on cells exposed to MP extract. However, EROD activity was induced and differently modulated depending on the MPs locations suggesting presence of different pollutants or additives in extract. DNA damage was observed after exposure to four microplastics samples on the six tested. Modification of EROD activity level and DNA damage rate highlight MPs extract toxicity on fish cell line.
Afficher plus [+] Moins [-]Microplastic contamination and pollutant levels in mussels and cockles collected along the channel coasts
2019
Hermabessiere, Ludovic | Paul Pont, Ika | Cassone, Anne-Laure | Himber, Charlotte | Receveur, Justine | Jezequel, Ronan | El Rakwe, Maria | Rinnert, Emmanuel | Rivière, Gilles | Lambert, Christophe | Huvet, Arnaud | Dehaut, Alexandre | Duflos, Guillaume | Soudant, Philippe
Nowadays, environmental pollution by microplastics (<5 mm; MP) is a major issue. MP are contaminating marine organisms consumed by humans. This work studied MP contamination in two bivalve species of commercial interest: blue mussel (Mytilus edulis) and common cockle (Cerastoderma edule) sampled on the Channel coastlines (France). In parallel, 13 plastic additives and 27 hydrophobic organic compounds (HOC) were quantified in bivalves flesh using SBSE-TD-GS-MS/MS to explore a possible relationship between their concentrations and MP contamination levels. MP were extracted using a 10% potassium hydroxide digestion method then identified by μ-Raman spectroscopy. The proportion of contaminated bivalves by MP ranged from 34 to 58%. Blue mussels and common cockles exhibited 0.76 ± 0.40 and 2.46 ± 1.16 MP/individual and between 0.15 ± 0.06 and 0.74 ± 0.35 MP/g of tissue wet weight. Some HOC and plastic additives were detected in bivalves. However, no significant Pearson or Spearman correlation was found between MP loads and plastic additives or HOC concentrations in bivalve tissues for the two species.
Afficher plus [+] Moins [-]Leachates of micronized plastic toys provoke embryotoxic effects upon sea urchin Paracentrotus lividus
2019
Oliviero, Maria | Tato, Tania | Schiavo, Simona | Fernández, Verónica | Manzo, Sonia | Beiras, Ricardo
Microplastics are defined as plastic fragments <5 mm, and they are found in the ocean where they can impact on the ecosystem. Once released in seawater, microplastics can be internalized by organisms due to their small size, moreover they can also leach out several additives used in plastic manufacturing, such as plasticizers, flame retardants, etc., resulting toxic for biota. The aim of this study was to test the toxicity of micronized PVC products with three different colors, upon Paracentrotus lividus embryos. In particular, we assessed the effects of micronized plastics and microplastic leachates. Results showed a decrease of larval length in plutei exposed to low concentrations of micronized plastics, and a block of larval development in sea urchin embryos exposed to the highest dose. Virgin PVC polymer did not result toxic on P. lividus embryos, while an evident toxic effect due to leached substances in the medium was observed. In particular, the exposure to leachates induced a development arrest immediately after fertilization or morphological alterations in plutei. Finally, PVC products with different colors showed different toxicity, probably due to a different content and/or combination of heavy metals present in coloring agents.
Afficher plus [+] Moins [-]Trace metals in polyethylene debris from the North Atlantic subtropical gyre
2019
Prunier, Jonathan | Maurice, Laurence | Perez, Emile | Gigault, Julien | Pierson Wickmann, Anne-Catherine | Davranche, Mélanie | Halle, Alexandra ter
Plastic pollution in the marine environment poses threats to wildlife and habitats through varied mechanisms, among which are the transport and transfer to the food web of hazardous substances. Still, very little is known about the metal content of plastic debris and about sorption/desorption processes, especially with respect to weathering. In this study, plastic debris collected from the North Atlantic subtropical gyre was analyzed for trace metals; as a comparison, new packaging materials were also analyzed. Both the new items and plastic debris showed very scattered concentrations. The new items contained significant amounts of trace metals introduced as additives, but globally, metal concentrations were higher in the plastic debris. The results provide evidence that enhanced metal concentrations increase with the plastic state of oxidation for some elements, such as As, Ti, Ni, and Cd. Transmission electron microscopy showed the presence of mineral particles on the surface of the plastic debris. This work demonstrates that marine plastic debris carries complex mixtures of heavy metals. Such materials not only behave as a source of metals resulting from intrinsic plastic additives but also are able to concentrate metals from ocean water as mineral nanoparticles or adsorbed species.
Afficher plus [+] Moins [-]Effects of Surface Active Additives on the Enzymatic Treatment of Phenol and Its Derivatives: a Mini Review
2019
Alshabib, Muntathir | Onaizi, Sagheer A.
PURPOSE OF REVIEW: Phenolic wastewaters represent a serious health and environmental problem. The remediation of phenolic wastewaters using oxidoreductase enzymes has emerged as an attractive environmentally friendly treatment method. However, the loss of enzyme activity during the treatment remains a key limitation. Thus, the aim of this article is to review and assess the recent progress in utilizing surface active additives (i.e., polymers, biopolymers, surfactants, and biosurfactants) for the reduction of enzyme inhibition and, thus, the enhancement of enzymatic remediation of phenolic wastewaters. RECENT FINDINGS: The reported effect of polymeric and surfactant additives on the enzymatic remediation of phenolic pollutants is mixed. Some studies reported significant enhancements while others demonstrated minimal or no gains. More seriously, it has been reported that these fossil-based additives might lead to a higher toxicity of the treated wastewaters. Bio-based (biopolymers and biosurfactants) additives might address this toxicity issue; however, the bio-based additives are not always as effective as the fossil-based ones. Despite the beneficial effect, with some exceptions, of additives, the enhancement level varies widely, probably due to the variations in the reaction environment. Thus, to draw meaningful and reliable conclusions on which additive(s) is more promising, thorough studies under unified conditions are needed. Additionally, generation of secondary pollutions associated with the fossil-based additives urges the replacement of such additives with bio-based ones. However, the effectiveness of the bio-based additives is still not sufficiently documented, stressing the need for more in-depth studies.
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