This study was performed to evaluate the toxic effects of aflatoxin B₂ (AFB₂) on antioxidant-oxidant balance and vital parameters such as physiological, cytogenetic, and anatomical alterations in Allium cepa L. root tip cells. Toxic effects of AFB₂ on vital parameters were investigated by using the changes in weight gain, germination percentage, chromosomal aberrations (CAs), mitotic index (MI), micronucleus frequency (MN), and anatomical structure. Malondialdehyde (MDA) and reduced glutathion (GSH) levels and superoxide dismutase (SOD) and catalase (CAT) activities in root cells were investigated as antioxidant-oxidant parameters. For this aim, A. cepa bulbs were seperated into five groups as negative control, positive control, and AFB₂ treatment groups. In results, while the rate of germination percentage, weight gain, and MI rates decreased, MN and CA frequency increased in AFB₂-treated groups compared with the negative control. Most common CAs observed in AFB₂-treated groups were fragment and chromosome bridges. It was determined that in 160 μg L⁻¹ AFB₂-treated group there was a 70.8% increase in MDA and a 78.1% decrease in GSH level compared with the negative control group and these changes indicate oxidative damage. In 160 μg L⁻¹ AFB₂ treatment group, SOD and CAT activities decreased importantly due to inhibition. In anatomical examinations, it was determined that AFB₂ treatment caused some anatomical damages in A. cepa root cells such as necrosis, cell deformation, and thickening in cell wall. This study showed that AFB₂, which has the least data among aflatoxins, causes serious in vivo toxic effects in A. cepa root cells.

The prevalence of aflatoxins (AFs) B₁, B₂, G₁, and G₂ in Iranian edible oils were assessed by immunoaffinity column cleanup and HPLC equipped with a fluorescence detector (HPLC-FLD). Ninety-seven samples including sunflower, canola, refined olive, unrefined olive, frying, and blend oils were collected from eight provinces (n = 15 samples of refined olive oil, n = 15 samples of unrefined olive oil, n = 15 samples of sunflower oil, n = 15 samples of canola oil, n = 17 samples of frying oil, and n = 20 samples of blend oil). Also, cancer risk of aflatoxins in the adults and children due to ingestion of edible oils was estimated via margin of exposure (MOE) estimation in the Monte Carlo simulation (MCS) model. Considering the limit of detection (LOD) of the current study, two unrefined olive oil samples from Zanjan Province were contaminated with AFB₂ in the concentrations of 0.2 and 0.4 ng/g while other samples were free from AFB₁, AFB₂, AFG₁, and AFG₂. This study revealed that about 98% of the collected samples were free from AFs and the concentrations of AFs in the polluted samples were within the standard range suggested by European Commission regulation (20 μg/kg). However, health risk assessment indicated that both adult and children in the Zanjan Province are at considerable liver cancer risk (percentile 95% of MOE < 10,000 value). Therefore, national plan to address this issue and strict inspection of edible oil products by the regulatory bodies are suggested.