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An environmental management technology for the processing of American aloe fibers using xylano-pectinolytic enzymes
2021
Kaur, Amanjot | Varghese, Libin Mathew | Battan, Bindu | Patra, Arun Kumar | Mandhan, Rishi Pal | Mahajan, Ritu
The objective of this research was to find out the potential of bioscouring using xylano-pectinolytic enzymes, for degumming of aloe fibers. Bioscouring was optimized with 1 : 20 fiber to buffer ratio, using 10 IU xylanase and 3.2 IU pectinase in 50 mM buffer (pH 8.5), EDTA (3 mM), and Tween 80 (1%), at 50°C temperature with agitation rate of 50 rpm and treatment period of 60 min. Enzymatic treatment of aloe fibers increased brightness and whiteness by 55.67% and 24.88%, respectively and decreased yellowness by 44.11% as compared to alkaline fiber scouring, thereby replacing chemical scouring completely. Additionally, the pretreatment of aloe fibers with enzymes resulted in a 50% less consumption of bleaching chemicals with similar optical properties as obtained by 100% bleaching. This is the first report showing the eco-friendly bioscouring approach of aloe fibers, using enzymes produced concurrently from a bacterial isolate.
Afficher plus [+] Moins [-]A rapid method to assess the formaldehyde dehydrogenase activity in plants for the remediation of formaldehyde
2021
He, Xiaohong | Li, Depeng | Ablikim, Ayxa | Yang, Yuxia | Su, Yuhong
The formaldehyde dehydrogenase (FADH) activity in plants is essential to the removal of airborne formaldehyde (FA) by plants. A rapid and efficient method was established to assess the FADH activity in plants by analyzing the efficiencies of the extracts of fresh and enzyme-inactivated leaves to degrade FA, with the enzyme-inactivated leaves prepared by freezing with liquid nitrogen. The efficiencies of airborne FA dissipated by different plants were evaluated through the FA fumigation experiments using four selected plants, with the results analyzed against the calculated leaf FADH activities. Fresh and enzyme-inactivated leaf extracts degraded FA to different extents. The degradative efficiencies of leaf extracts were positively related to the initial FA test levels at 6–18 mg l⁻¹. The relative plant-leaf FADH activities formed the order of Chenopodium album L. > Atenia cordifolia > Plantain > Aloe, which was in line with the observed FA dissipating efficiencies of the plants exposed to 0.72 mg m⁻³ airborne FA for 24 h. Other dominant degrading mechanisms in plant leaves resulted in higher dissipating efficiencies of Plantain over that of Atenia cordifolia when exposed to 1.56 mg m⁻³ FA for 24 h. The established method could be applied to estimate the FADH activity in plants for assessment of the plant remediation efficiency of FA in air at lower concentrations.
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