This study carried out to investigate the production of hydrogen using the organic fraction of municipal solid waste OFMSW, where the anaerobic digester was depended as a method for disposing and treating OFMSW and producing bio-hydrogen. Bio-hydrogen production had been studied under different parameters including pH, solid content T.S%, temperature and mixing ratios between the thick sludge to OFMSW. The optimal conditions were found at pH, T.S%, temp and mix ratio of 7, 8%, 32oC, and 1:5, respectively where the hydrogen yield was (138.88 mL/gm vs).  To found the most important parameters in this process, the ANN model had been applied. The effectiveness of temperature, total solid, mixing ratio and pH comes in the following sequence 100%, 75.8%, 71.9%, and 57.2% respectively, with R2 of 95.7%. Multiple correlation model was used to formulate an equation linked between the hydrogen production and the parameters effected on. Gompertz model was applied to compare between theoretical and experimental outcomes, it also given a mathematical equation with high correlation coefficient R2 of 99.95% where the theoretical bio-hydrogen was (141.76 mL/gm vs) under best conditions. The first order kinetic model was applied to evaluate the dynamics of the degradation process. The obtained negative value of (k = - 0.0886), indicates that, the solid waste biodegradation was fast and progresses in the right direction.

The present study investigates the influence of individual and combined hydrogen peroxide and thermal pre-treatment of waste activated sludge on anaerobic digestion. For so doing, it employs anaerobic batch reactors in the mesophilic conditions. For comparison, soluble fractions of organic matter, biogas production, biochemical methane potential, removal of chemical oxygen demand (COD), and volatile solids (VS) have been measured during the anaerobic digestion process in systems with and without pre-treatment. Hydrogen peroxide pre-treatment has been tested in two concentrations of 30 g H2O2/kg VS and 60 g H2O2/kg VS and thermal pre-treatment has been performed at two temperatures of 75℃ and 90℃. According to the results, the solubalisation of organic matter considerably improves, when combined hydrogen peroxide and thermal pre-treatment is employed. As a result, in comparison to the control reactor, higher amounts of biogas (71%) and methane (81%) have been produced in the bioreactor, pre-treated with combined hydrogen peroxide (30 g H2O2/kg VS) and heat (90 ℃). In addition, the removal efficiency of COD and VS from the digested sludge has been enhanced in the pre-treated reactors (up to 39% and 92%, respectively) in comparison to the control reactor. The improved methane yield, COD, and VS are of paramount importance, not only because higher amounts of renewable energy are obtained from the anaerobic digestion process, but because sludge transport costs are reduced and the digested sludge obtains a higher potential application to agricultural lands.