In order to carry out the program of protection and rational exploitation of Kovilj Marsh (Serbia, Yugoslavia), which is a part of special natural reserve called the Marsh of Kovilj and Petrovaradin, we have done an ecological analysis of the fish community. The research was conducted during the period of "low water", in the autumn 2000, on the following locations: Slajz, Tonja and Arkanj, and we have established the presence of 12 species from 6 different families. The saprobic index of the majority of bioindicative species shows that the water belongs to the beta-mesosaprobic type (the saprobic index according to Pantle-Buck is 2.1). The presence and abundance of certain ecological groups of fish is examined in relation to migration, type of food and type of substrate on which the fish lay their eggs. The results of this examination point to main features of certain localities in this floodplain, as well as to the necessity of taking appropriate measures of protection of this valuable water resource.

The investigations have been done in Krstonosica shaft at 3 points, in February, May and October 1995, and in January and April 1996. They included algae, Rotatoria, Copepoda, Cladocera, and Hydracarina. The species from divisio Bacillariophyta were presented most among the algal species, with 56 species, then from divisio Chlorophyta, with 26 taxons, Euglenophyta 11, Cyanobacteria 9, Pyrrophyta 3, Xanthophyta 2 and Chrysophyta 1. Among animal groups the highest number belonged to the Rotatoria group, 111 taxons have been determined. The highest number was found during autumn season (86), then in spring (73), and only 14 species in winter. The species that mostly prevailed were phytophyl species. The Cladocera group was present with 18 taxons. The phytophyl species from overgrown plants again prevailed. The Copepoda group was present with 10 species and the phytophyl species were prevailed. According to saprobiological characteristics the greatest number belongs to beta-mezosaprobionts, then oligosaprobionts. A small number of species indicates to the eutrophication process and organic pollution of water.

The main characteristics of catchment area of Boka Kotorska Bay (Adriatic sea, Montenegro, Yugoslavia), are high slope, carstic structure of ground, high precipitation (over 5000 mm a year) and high run-off of rain water. All rainwater is transported trough carstic ground structures quickly appearing in numerous sources along the sea cost or ones located on the bottom of the Bay. The large volume of freshwater which permanently empty into the Bay has the strong influence on the aquatic environment (salinity, temperature, transparency, etc.) and biota (abundance of the species) as it has been observed in the parts of Boka Kotorska Bay (i.e. Morinj and Risan Bays). The abundance and number of species are the good indicator of changes of aquatic environment and hydrological changes as well as of the capability of aquatic organisms to adapt quickly on the severe changes of conditions.

The worldwide use of pesticides has led to increases in agricultural yields by reducing crop losses. However, increased pesticide use has resulted in pesticide-resistant pest species and recent studies have discovered pesticide-resistance in non-target species living close to farms. Such increased tolerance not only affects the species, but can alter the entire food web. Given that some species can evolve not only resistance to a single pesticide, but also cross-resistance to other pesticides that share the same mode of action, one would predict that cross-resistance to pesticides would also have effects on the entire community and affect community stability. To address this hypothesis, we conducted an outdoor mesocosm experiment comprised of 200 identical aquatic communities with phytoplankton, periphyton, and leopard frog (Lithobates pipiens) tadpoles. To these communities, we added one of four Daphnia pulex populations that we previously discovered were either resistant or sensitive to the insecticide of chlorpyrifos as a result of living close to or far from agriculture, respectively. We then exposed the communities to either no insecticide or three different concentrations of AChE-inhibiting insecticides (chlorpyrifos, malathion or carbaryl) or sodium channel-inhibiting insecticides (permethrin or cypermethrin). We discovered that communities containing sensitive Daphnia pulex experienced phytoplankton blooms and subsequent cascades through all trophic groups including amphibians at moderate to high concentrations of all five insecticides. However, communities containing resistant D. pulex were buffered from these effects at low to moderate concentrations of all AChE-inhibiting insecticides, but were not buffered against the pyrethroid insecticides. These data suggest that a simple change in the population-level resistance of zooplankton to a single insecticide can have widespread consequences for community stability and that the effects can be extrapolated to a wide variety of pesticides that share the same mode of action.

The application of road deicing salts has led to the salinization of freshwater ecosystems in northern regions worldwide. Increased chloride concentrations in lakes, streams, ponds, and wetlands may negatively affect freshwater biota, potentially threatening ecosystem services. In an effort to reduce the effects of road salt, operators have increased the use of salt alternatives, yet we lack an understanding of how these deicers affect aquatic communities. We examined the direct and indirect effects of the most commonly used road salt (NaCl) and a proprietary salt mixture (NaCl, KCl, MgCl2), at three environmentally relevant concentrations (150, 470, and 780 mg Cl−/L) on freshwater wetland communities in combination with one of three biotic stressors (control, predator cues, and competitors). The communities contained periphyton, phytoplankton, zooplankton, and two tadpole species (American toads, Anaxyrus americanus; wood frogs, Lithobates sylvaticus). Overall, we found the two road salts did not interact with the natural stressors. Both salts decreased pH and reduced zooplankton abundance. The strong decrease in zooplankton abundance in the highest NaCl concentration caused a trophic cascade that resulted in increased phytoplankton abundance. The highest NaCl concentration also reduced toad activity. For the biotic stressors, predatory stress decreased whereas competitive stress increased the activity of both tadpole species. Wood frog survival, time to metamorphosis, and mass at metamorphosis all decreased under competitive stress whereas toad time to metamorphosis increased and mass at metamorphosis decreased. Road salts and biotic stressors can both affect freshwater communities, but their effects are not interactive.

Ametryne is an herbicide applied to sugar cane cultures to prevent the emergence of weeds. It is a persistent compound that percolates ground and surface water thus impacting aquatic communities. In this study, we evaluated microbial activity in soil with increased concentrations of ametryne solution and commercial Microgeo biofertilizer. The soil subject to analysis was obtained from a sugar cane cultivation area. The concentration used in the experiment was ametryne 12 μg/L and 1 % of biofertilizer. It was used with the Bartha and Pramer respirometric method to quantify CO₂production and determine microbial activity. Complimentary phytotoxicity tests with Lactuca sativa seeds after respirometry experiments were conducted in the soluble fraction of the soil. According to the results, the addition of biofertilizer promoted microbial activity in the presence of ametryne and reduced ametryne phytotoxicity for Lactuca sativa seeds. Thus, Microgeo biofertilizer can potentially improve biodegradation of ametryne through both bioaugmentation and bioestimulation.

Aquatic risk assessments for fungicides are carried out without information on their toxicity to non-target aquatic fungi. This might cause an underestimation of the toxic effects to the aquatic fungal community. This study focuses on the question whether recently derived concentrations limits for fungicides considered to protect populations of primary producers and (in)vertebrates also do protect the aquatic fungi. A panel of fungal species and Oomycetes was isolated and identified from unpolluted surface waters in the Netherlands. Toxicity tests were used to determine effects of seven fungicides with different modes of actions. For the triazoles epoxiconazole and tebuconazole, the chronic lowest observable effect concentration was lower than the regulatory acceptable concentration based on acute HC5 values.