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Résultats 11-20 de 62
Toxicity of two representative petroleum hydrocarbons, toluene and phenanthrene, to five Atlantic coral species
2021
Turner, Nicholas R. | Parkerton, Thomas F. | Renegar, D Abigail
Coral reefs are keystone coastal ecosystems that can be exposed to petroleum hydrocarbons from multiple sources, and when selecting spill response methods to limit environmental damages, corals represent one of the highest valued resources for protection. Because previous research to characterize the sensitivity of coral species to petroleum hydrocarbon exposures is limited, a continuous-flow passive dosing system and toxicity testing protocol was designed to evaluate the acute effects of two representative petroleum compounds, toluene and phenanthrene, on five coral species: Acropora cervicornis, Porites astreoides, Siderastera siderea, Stephanocoenia intersepta, and Solenastrea bournoni. Using analytically confirmed exposures, sublethal and lethal endpoints were calculated for each species, and used as model inputs to determine critical target lipid body burdens (CTLBBs) for characterizing species sensitivity. Further, quantification of the time-dependent toxicity of single hydrocarbon exposures is described to provide model inputs for improved simulation of spill impacts to corals in coastal tropical environments.
Afficher plus [+] Moins [-]Degradation of Deepwater Horizon oil buried in a Florida beach influenced by tidal pumping
2018
Huettel, Markus | Overholt, Will A. | Kostka, Joel E. | Hagan, Christopher | Kaba, John | Wells, Wm Brian | Dudley, Stacia
After Deepwater Horizon oil reached the Florida coast, oil was buried in Pensacola Beach (PB) sands to ~70cm depth, resulting in Total Petroleum Hydrocarbon (TPH) concentrations up to ~2kg per meter of beach. This study followed the decomposition of the buried oil and the factors influencing its degradation. The abundance of bacteria in oiled sand increased by 2 orders of magnitude within one week after oil burial, while diversity decreased by ~50%. Half-lives of aliphatic and aromatic hydrocarbons reached 25 and 22days, respectively. Aerobic microbial oil decomposition, promoted by tidal pumping, and human cleaning activities effectively removed oil from the beach. After one year, concentrations of GC-amenable hydrocarbons at PB were similar to those in the uncontaminated reference beach at St. George Island/FL, and microbial populations that disappeared after the oil contamination had reestablished. Yet, oxihydrocarbons can be found at PB to the present day.
Afficher plus [+] Moins [-]Microbial communities in seawater from an Arctic and a temperate Norwegian fjord and their potentials for biodegradation of chemically dispersed oil at low seawater temperatures
2018
Ribicic, Deni | Netzer, Roman | Winkler, Anika | Brakstad, Odd Gunnar
Biodegradation of chemically dispersed oil at low temperature (0–2 °C) was compared in natural seawater from Arctic (Svalbard) and a temperate (Norway) fjords. The oil was premixed with a dispersant (Corexit 9500) and small-droplet oil dispersions prepared. Faster biotransformation of n-alkanes in the Arctic than in the temperate seawater were associated with the initially higher abundance of the alkane-degrading genus Oleispira in the Arctic than the temperate seawater. Comparable transformation of aromatic hydrocarbons was further associated with the late emergences Cycloclasticus in both seawater sources. The results showed that chemically dispersed oil may be rapidly biodegraded by microbial communities in Arctic seawater. Compared to oil biodegradation studies at higher seawater temperatures, longer lag-periods were experienced here, and may be attributed to both microbial and oil properties at these low seawater temperatures.
Afficher plus [+] Moins [-]Isolation and characterization of two crude oil-degrading yeast strains, Yarrowia lipolytica PG-20 and PG-32, from the Persian Gulf
2012
Hassanshahian, Mehdi | Tebyanian, Hamid | Cappello, Simone
Among six crude oil-degrading yeasts that were isolated from an oil-polluted area in the Persian Gulf, two yeast strains showed high degradation activity of aliphatic hydrocarbons. From an analysis of 18S rRNA sequences and biochemical characteristics, these strains were identified as Yarrowia lipolytica strains PG-20 and PG-32. Gas Chromatography (GC) analysis of the crude oil remaining in the culture medium after 1week at 30°C showed that the strains PG-20 and PG-32 degraded 68% and 58% of crude oil, respectively. The optimal growth condition and biodegradation of hydrocarbons was in ONR medium with an acidic pH (pH5). These two strains may degrade aliphatic hydrocarbons more efficiently than aromatic hydrocarbons, although strain PG-20 had better degradation than strain PG-32. The two Y. lipolytica strains reduce surface tension when cultured on hydrocarbon substrates (1% v/v). These strains showed a cell surface hydrophobicity higher than 70%. These results suggested that Y. lipolytica strains PG-20 and PG-32 have high crude oil degrading activity due to their high emulsifying activity and cell hydrophobicity. In conclusion, these yeast strains can be useful for the bioremediation process in the Persian Gulf and decreasing oil pollution in this marine ecosystem.
Afficher plus [+] Moins [-]Miniaturised marine tests as indicators of aromatic hydrocarbon toxicity: Potential applicability to oil spill assessment
2021
Colvin, Katherine A. | Parkerton, Thomas F. | Redman, Aaron D. | Lewis, Ceri | Galloway, Tamara S.
Assessing oil spill toxicity in real time is challenging due to dynamic field exposures and lack of simple, rapid, and sensitive tests. We investigated the relative sensitivity of two commercially available marine toxicity tests to aromatic hydrocarbons using the target lipid model (TLM). State of the art passive dosing in sealed vials was used to assess the sensitivity of brine shrimp (Artemia franciscana) and rotifer (Brachionus plicatilis). Organisms were exposed to toluene, 1-methylnaphthalene and phenanthrene for 24 h. Toxicity results were analysed using the TLM to estimate the critical target lipid body burden and support comparison to empirical data for 79 other aquatic organisms. Our findings demonstrate the applicability of passive dosing to test small volumes and indicate that the two rapid cyst-based assays are insensitive in detecting hydrocarbon exposures compared to other aquatic species. Our results highlight the limitations of applying these tests for oil pollution monitoring and decision-making.
Afficher plus [+] Moins [-]The sensitivity of the deepsea species northern shrimp (Pandalus borealis) and the cold-water coral (Lophelia pertusa) to oil-associated aromatic compounds, dispersant, and Alaskan North Slope crude oil
2020
Bytingsvik, Jenny | Parkerton, Thomas F. | Guyomarch, Julien | Tassara, Luca | LeFloch, Stephane | Arnold, W Ray | Brander, Susanne M. | Volety, Aswani | Camus, Lionel
This study investigated the sensitivity of two deepsea species using mortality of northern shrimp (Pandalus borealis) and polyp activity of stony coral (Lophelia pertusa) to dispersant, Corexit 9500 and aromatic hydrocarbons (toluene, 2-methylnaphthalene, phenanthrene) in 96-h tests. Resulting hydrocarbon toxicity data were fit to the Target Lipid Model to generate predictive models and determine species sensitivity. Toxicity of chemically enhanced water accommodated fractions of Alaskan North Slope crude oil (ANS-oil) was also investigated with shrimp using nominal loading, total petroleum hydrocarbons and biomimetic extraction (BE) as oil exposure metrics. Coral were more sensitive to dispersant than shrimp while similar sensitivity was observed for hydrocarbons. Study and literature findings indicate deepsea species exhibit acute sensitivities to dispersant, hydrocarbons and oil that are comparable to pelagic species. Results support use of passive sampling methods to quantify dissolved oil for interpreting oil toxicity tests and suggest models for predicting time-dependence of toxicity warrant re-evaluation.
Afficher plus [+] Moins [-]Macondo oil in northern Gulf of Mexico waters – Part 1: Assessments and forensic methods for Deepwater Horizon offshore water samples
2018
Payne, James R. | Driskell, William B.
Forensic chemistry assessments documented the presence of Macondo (MC252) oil from the Deepwater Horizon (DWH) spill in offshore water samples collected under Natural Resource Damage Assessment (NRDA) protocols. In ocean depths, oiled water was sampled, observed, photographed, and tracked in dissolved oxygen (DO) and fluorometry profiles. Chemical analyses, sensor records, and observations confirmed the shifting, rising oil plume above the wellhead while smaller, less buoyant droplets were entrapped in a layer at ~1000–1400 m and advected up to 412 km southwest. Near-surface oil samples showed substantial dissolution weathering from oil droplets rising through the water column, as well as enhanced evaporative losses of lighter n-alkanes and aromatic hydrocarbons. Dispersant effects from surface applications and injected at the wellhead were seen in oil profiles as enhanced weathering patterns (increased dissolution), thus implying dispersants were a functionally effective mediation treatment. Forensic assessment methods are detailed in the Supplemental information (SI).
Afficher plus [+] Moins [-]The traffic linked urban ambient air superfine and ultrafine PM1 mass concentration, contents of pro–oxidant chemicals, and their seasonal drifts in Lucknow, India
2014
Verma, Mukesh Kumar | Singh Chauhan, Lalit Kumar | Sultana, Sarwat | Kumar, Sushil
The anthropogenic PM2.5 in ambient air is implicated in the increased health risks and morbidities of urban subjects. However in the literature, there is a limited information on mass concentrations and size segregated chemical profile of ultrafine and superfine PM1 fractions of traffic linked urban ambient air. The size, and the adsorbed chemicals, and response to seasonal change in this submicron range of particles have seldom been studied so far. We have looked into these aspects in urban ambient air of Lucknow Uttar Pradesh India. A 10–stage MOUDI cascade impactor sampled the superfine (PM0.56, PM0.32, PM0.18) and ultrafine particles (PM0.1, PM0.056) distributed in traffic linked ambient air. We studied their morphology, mass concentrations, the adsorbed metal contents and organic chemical moieties using TEM, gravimetric analyses, AAS, and FTIR spectroscopy. A change in their contents and profile with season was also examined. Results revealed spherical and fractal shapes of PM0.1, 50nm–2μm size range of PM0.56 fractions, and 10–100nm size range of constituent spherules. Gravimetric analyses disclosed mass concentrations and multifold increases in their levels in winter. Cr, Cd, Ni, Pb and transition element Cu, Fe were found to be present in the studied particles. The presence of aliphatic and aromatic hydrocarbons with hydroxyl, carbonyl, and ketone groups were also found and displayed changes in their levels with season. Presence of organonitrate compounds indicated the role of submicron and nanosize particulates in secondary aerosol formation also. Results are important for epidemiological studies and public policy on superfine and ultrafine particulate matter in urban ambient air for identification of toxicity risk or health hazard, air quality monitoring and regulation.
Afficher plus [+] Moins [-]The influence of Corexit 9500A and weathering on Alaska North Slope crude oil toxicity to the ammonia oxidizing bacterium, Nitrosomonas europaea
2013
Radniecki, Tyler S. | Schneider, Margaret C. | Semprini, Lewis
The toxicity of the water associated fraction (WAF) of Alaska North Slope Crude oil (ANSC), Corexit 9500A and the dispersant enhanced WAF (DEWAF) of ANSC:Corexit 9500A mixtures were examined on the model ammonia oxidizing bacterium, Nitrosomonas europaea. Corexit 9500A was not toxic at environmentally relevant concentrations. Corexit 9500A greatly increased the toxicity of ANSC by increasing the chemical oxygen demand (COD) of the DEWAF. However, a majority of the DEWAF compounds were not toxic to N. europaea. Weathered WAF and DEWAF were not toxic to N. europaea even though their COD did not change compared to non-weathered controls, suggesting that toxicity was due to a small volatile fraction of the ANSC. The over-expression of the NE1545 gene, a marker for aromatic hydrocarbon exposure, in N. europaea cells exposed to WAF and DEWAF suggests that aromatic hydrocarbons are bioavailable to the cells and may play a role in the observed toxicity.
Afficher plus [+] Moins [-]Contaminants assessment in the coral reefs of Virgin Islands National Park and Virgin Islands Coral Reef National Monument
2013
Bargar, Timothy A. | Garrison, Virginia H. | Alvarez, David A. | Echols, Kathy R.
Coral, fish, plankton, and detritus samples were collected from coral reefs in Virgin Islands National Park (VIIS) and Virgin Islands Coral Reef National Monument (VICR) to assess existing contamination levels. Passive water sampling using polar organic chemical integrative samplers (POCIS) and semi-permeable membrane devices found a few emerging pollutants of concern (DEET and galaxolide) and polynuclear aromatic hydrocarbons. Very little persistent organic chemical contamination was detected in the tissue or detritus samples. Detected contaminants were at concentrations below those reported to be harmful to aquatic organisms. Extracts from the POCIS were subjected to the yeast estrogen screen (YES) to assess potential estrogenicity of the contaminant mixture. Results of the YES (estrogen equivalency of 0.17–0.31ng/L 17-β-estradiol) indicated a low estrogenicity likelihood for contaminants extracted from water. Findings point to low levels of polar and non-polar organic contaminants in the bays sampled within VICR and VIIS.
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