Paint particles are part of the increasingly important microplastics (MPs) pollution of our oceans. They contain polyurethanes, polyesters, polyacrylates, polystyrenes, alkyls and epoxies. In spite of their prevalence, paint fragments are often excluded from MP audits. This review, citing 127 references, discusses detection, characteristics, sources and ecological effects of paint fragments in our oceans, as well as the abundance of paint fragments in MP samples around the world and their colonization by marine microorganisms, which differs from that of non-paint MPs. Paint MPs arise from shipping and boating activities, road markings and external surfaces of buildings. Many paint fragments come from antifouling paints used on commercial vessels and leisure boats; these may be regarded as particular pollutants, not only containing but also leaching heavy metals and biocides. Some effects of antifouling paint particles on aquatic biota are caused by these toxins. Paint particles are an understudied portion of marine MP pollution.

The contamination by antifouling biocide residues (booster biocides - diuron, Irgarol, chlorothalonil, dichlofluanid and DCOIT; butyltin compounds-BTs (TBT, DBT and MBT); and antifouling paint particles-APPs) was appraised in sediments of Vitoria Estuarine System (VES). Even at its historical lower (ΣBTs ≤113 ng Sn g⁻¹ dry wt), the current environmental levels of BTs in areas with a predominance of boatyards still pose a risk to the local biota and human population. DCOIT, among booster biocides, was the most frequently detected, especially in boatyards (≤40 ng g⁻¹ dry wt) and Vitoria Port (64 ng g⁻¹ dry wt), while APPs were also detected mainly in sediments of boatyards (≤5,969 μg g⁻¹ dry wt). Since levels of diuron and DCOIT in APPs were as high as 1,670,000 and 899,000 ng g⁻¹ dry wt, respectively, they are acting as secondary sources of these antifouling biocides. Therefore, VES is threatened by antifouling biocide residues due to the multiple diffuse sources of contamination, showing the need for more efforts on public policies (including temporal trend monitoring studies).

Triphenyltin (TPT) has been known as one of the most toxic compounds being released into the marine environment by anthropogenic means. This study assessed the contamination statuses of TPT and its two major degradants, i.e., monophenyltin and diphenyltin, in seawater, sediment and biota samples from marine environments of Hong Kong, a highly urbanized and densely populated city, and evaluated their ecological and human health risks. The results showed that the Hong Kong's marine environments were heavily contaminated with these chemicals, especially for TPT. Concentration ranges of TPT in seawater, sediment and biota samples were 3.8–11.7 ng/L, 71.8–91.7 ng/g d.w., and 9.6–1079.9 ng/g w.w., respectively. As reflected by high hazard quotients (1.7–5.3 for seawaters; 46.1–59.0 for sediments), TPT exhibited high ecological and human health risks. Our results are essential for the future management and control of anthropogenic TPT use in antifouling paints and as biocides in agriculture.

A risk assessment framework for direct exposure of residual additives and monomers present in ingested plastic particles, including microplastics, in the Danish marine environment, was presented. Eight cases of different polymer types and product groups were defined that represent the most significant exposures, and thus potential high-risk cases, towards marine organisms. Risk Quotients (RQ) were calculated for three trophic levels, i.e. pelagic/planktonic zooplankton: copepod, benthopelagic fish: Atlantic cod and seabird: northern fulmar. European and Danish Environmental Quality Standard (EQS) values were used as Predicted No-Effect Concentrations (PNEC). RQ larger than unity, indicating potential risks, were found for copepod and cod (pelagic community) and the flame-retardant pentabromodiphenyl ether (PeBDE) used in polyurethane (PUR), the biocide tributyltin (TBT) present as impurity in polyvinylchloride (PVC) and PUR, and the flame-retardant hexabromocyclododecane (HBCDD) used in expanded polystyrene (EPS). A potential risk was found for fulmar (secondary poisoning) and PeBDE used in PUR.

A simultaneous monitoring study on organotins (butyltins and phenyltins) and most frequently used alternative antifouling biocides (Irgarol 1051, Diuron, Sea-Nine 211 and M1) in water and sediments (n=44) collected from three Special Management Sea Areas operated by Korean government. The lower concentration of butyltins (BTs) than that of new antifouling biocides (NEW) was found in water but the significant greater concentration of BTs than that of NEW was still found in sediments. The tributyltin (TBT) levels in water exceeded the chronic criterion to protect seawater aquatic life at several sites. Even ten years after the ban of the use of TBT-based antifouling paint, the concentrations of TBT, Diuron and Irgarol 1051 in sediments from shipyards exceeded global sediment quality guidelines and potentially poses adverse risks on marine organisms and extremely high concentration of TBT up to 2304ng/g was found for a sediment collected at a shipyard.

Chlorine dioxide (ClO2) is seen as an effective alternative to chlorine, which is widely used as an antifouling biocide. However, data on its efficacy against marine macrofoulants is scanty. In this study, acute toxicity of ClO2 to larval forms of the fouling barnacle Amphibalanus reticulatus was investigated. ClO2 treatment at 0.1mg/L for 20min elicited 45–63% reduction in naupliar metamorphosis, 70% inhibition of cyprid settlement and 80% inhibition of metamorphosis to juveniles. Increase in concentration to 0.2mg/L did not result in any significant difference in the settlement inhibition or metamorphosis. Treatment with 0.2mg/L of ClO2 elicited substantial reduction in the settlement of barnacle larvae compared to control. The study indicates the possibility of using ClO2 as an alternative antifouling biocide in power plant cooling water systems. However, more work needs to be done on the environmental effects of such switchover, which we are currently undertaking.

2-methythiol-4-tert-butylamino-6-cyclopropylamino-s-triazine (Irgarol-1051) has been widely used as effective alternative antifouling paint in marine structures including ships. However, it has been causing deleterious effects to marine organisms including reef building corals. The main objective of this study was to establish baseline levels of Irgarol-1051 around coral reefs and nearby ecosystems along coastline of Zanzibar Island. The levels of Irgarol-1051 ranged from 1.35ng/L around coral reefs to 15.44ng/L around harbor with average concentration of 4.11 (mean)±0.57 (SD) ng/L. This is below Environmental Risk Limit of 24ng/L as proposed by Dutch Authorities which suggests that the contamination is not alarming especially for coral reef ecosystem health. The main possible sources of the contamination are from shipping activities. This paper provides important baseline information of Irgarol-1051 around the coral reef ecosystems within the Western Indian Ocean (WIO) region and may be useful for formulation of marine conservation strategies and policies.

Turbinaria ornata mediated silver nanoparticles (TOAg-NPs) were evaluated for antibacterial activity against 15 biofilm forming bacterial isolates. A field study in natural seawater for 60days showed antifouling activity of TOAg-NPs on stainless steel coupons (SS-304) coated with Apcomin zinc chrome (AZC) primer. Though TOAg-NPs showed broad spectrum of antibacterial activity, the maximum zone of inhibition was with Escherichia coli (71.9%) and a minimum with Micrococcus sp. (40%) due to the EPS secretion from Gram-positive bacteria. Compared to control coupons (18.9 [×103], 67.0 [×103], 13.5 [×104] and 24.7 [×104]CFU/cm2), experimental biocide coupons (71.0 [×102], 32.0 [×103], 82.0 [×103] and 11.3 [×104]CFU/cm2) displayed lesser bacterial population density. Toxicity studies revealed 100% mortality for Balanus amphitrite larvae at 250μgml−1 concentration within 24h, while 56.6% recorded for Artemia marina at the same concentration indicating less toxicity to non target species. It proved that AZC+TOAg-NPs prevent biofouling by its Ag-NS affinity and antimicrobial effectivity.

This study investigated the sublethal effects of environmentally relevant concentrations of DCOIT on the neotropical oyster Crassostrea brasiliana. Gills and digestive glands of animals exposed to increasing concentrations of DCOIT were analyzed for biochemical, cellular, and histopathological responses. Exposure to DCOIT (0.2 to 151 μg L⁻¹) for 120 h triggered oxidative stress in both tissues (through the modulation of GPX, GST, GSH and GR), which led to damage of membrane lipids (increase of LPO and reduction of the NRRT). DCOIT increased histopathological pathologies in gills, such as necrosis, lymphocyte infiltration and epithelial desquamation. This study showed that short term exposure to environmental concentrations of DCOIT causes negative effects on C. brasiliana at biochemical, physiological, and histological levels. Therefore, the use of DCOIT as a booster biocide in antifouling paints should be further assessed, as it may cause environmental hazards to marine organisms.