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Enriching for microbial reductive dechlorination of polychlorinated dibenzo-p-dioxins and dibenzofurans
2014
Liu, Hui | Park, Joong-Wook | Häggblom, Max M.
Anaerobic enrichment cultures derived from contaminated Kymijoki River sediments dechlorinated 1,2,3,4-tetrachlorodibenzofuran (1,2,3,4-tetra-CDF), octachlorodibenzofuran (octa-CDF) and 1,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-tetra-CDD). 1,2,3,4-tetra-CDF was dechlorinated via 1,2,3-, 2,3,4-, and 1,3,4/1,2,4-tri-CDFs to 1,3-, 2,3-, and 2,4-di-CDFs and finally to 4-mono-CDF. The dechlorination rate of 1,2,3,4-tetra-CDF was generally slower than that of 1,2,3,4-tetra-CDD. The rate and extent of 1,2,3,4-tetra-CDD dechlorination was enhanced by addition of pentachloronitrobenzene (PCNB) as a co-substrate. Dechlorination of spiked octa-CDF was observed with the production of hepta-, hexa-, penta- and tetra-CDFs over 6 months. Two major phylotypes of the Chloroflexi community showed an increase, one of which was identical to the Dehalococcoides mccartyi Pinellas subgroup. A set of twelve putative reductive dehalogenase (rdh) genes increased in abundance with addition of 1,2,3,4-tetra-CDF, 1,2,3,4-tetra-CDD and/or PCNB. This information will aid in understanding how indigenous microbial communities impact the fate of PCDFs and in developing strategies for bioremediation of PCDD/F contaminated sediments.
Afficher plus [+] Moins [-]Investigation of microbial community structure in constructed mangrove microcosms receiving wastewater-borne polycyclic aromatic hydrocarbons (PAHs) and polybrominated diphenyl ethers (PBDEs)
2014
Wang, Yafen | Wu, Yan | Bi, Na | Tam, Nora Fung-yee
The study aims to examine relationships between microbial community structure and mixed pollutants of polycyclic aromatic hydrocarbons (PAHs) and polybrominated diphenyl ethers (PBDEs) in constructed wetland microcosms, planted with Excoecaria agallocha or Kandelia obovata, two common mangrove plant species, and under two tidal regimes, everyday tidal (Te) and no tidal flooding (Tn). Results showed both microbial community structure and the retained amounts of pollutants were significantly determined by tidal regime, while the effect of plant species was small. Higher amounts of PAHs but lower amounts of PBDEs were always retained in sediments under Te than Tn regimes. Accordingly, temporal and vertical distributions of microbial community structure differed greatly between the two tidal regimes. Redundancy analysis further revealed significant correlation between a subgroup of the mixed PAHs and PBDEs with variation in microbial community structure. The findings will help to propose specific strategies to improve the bioremediation efficiency of constructed wetland.
Afficher plus [+] Moins [-]Bioremediation potential of microorganisms derived from petroleum reservoirs
2014
Dellagnezze, Bruna Martins | de Sousa, Gabriel Vasconcelos | Martins, Laercio Lopes | Domingos, Daniela Ferreira | Limache, Elmer E.G. | de Vasconcellos, Suzan Pantaroto | da Cruz, Georgiana Feitosa | de Oliveira, Valéria Maia
Bacterial strains and metagenomic clones, both obtained from petroleum reservoirs, were evaluated for petroleum degradation abilities either individually or in pools using seawater microcosms for 21days. Gas Chromatography–Flame Ionization Detector (GC–FID) and Gas Chromatography-Mass Spectrometry (GC–MS) analyses were carried out to evaluate crude oil degradation. The results showed that metagenomic clones 1A and 2B were able to biodegrade n-alkanes (C14 to C33) and isoprenoids (phytane and pristane), with rates ranging from 31% to 47%, respectively. The bacteria Dietzia maris CBMAI 705 and Micrococcus sp. CBMAI 636 showed higher rates reaching 99% after 21days. The metagenomic clone pool biodegraded these compounds at rates ranging from 11% to 45%. Regarding aromatic compound biodegradation, metagenomic clones 2B and 10A were able to biodegrade up to 94% of phenanthrene and methylphenanthrenes (3-MP, 2-MP, 9-MP and 1-MP) with rates ranging from 55% to 70% after 21days, while the bacteria Dietzia maris CBMAI 705 and Micrococcus sp. CBMAI 636 were able to biodegrade 63% and up to 99% of phenanthrene, respectively, and methylphenanthrenes (3-MP, 2-MP, 9-MP and 1-MP) with rates ranging from 23% to 99% after 21days. In this work, isolated strains as well as metagenomic clones were capable of degrading several petroleum compounds, revealing an innovative strategy and a great potential for further biotechnological and bioremediation applications.
Afficher plus [+] Moins [-]Microbiological accumulation by the Mediterranean invasive alien species Branchiomma bairdi (Annelida, Sabellidae): Potential tool for bioremediation
2014
Stabili, Loredana | Licciano, Margherita | Lezzi, Marco | Giangrande, Adriana
We examined the bacterial accumulation and digestion in the alien polychaete Branchiomma bairdi. Microbiological analyses were performed on worm homogenates from “unstarved” and “starved” individuals and on seawater from the same sampling site (Ionian Sea, Italy). Densities of culturable heterotrophic bacteria (22°C), total culturable bacteria (37°C) and vibrios were measured on Marine Agar 2216, Plate Count Agar and TCBS Agar, respectively. Microbial pollution indicators were determined by the most probable number method. B. bairdi was able to accumulate all the six considered microbiological groups which, however, differ in their resistance to digestion. B. bairdi results more efficient than the other two co-occurring sabellids in removing bacteria suggesting that it may counteract the effects of microbial pollution playing a potential role for in situ bioremediation. Thus a potential risk, such as the invasion of an alien species, could be transformed into a benefit with high potential commercial gain and economic feasibility.
Afficher plus [+] Moins [-]Uptake of PCBs contained in marine sediments by the green macroalga Ulva rigida
2014
The uptake of PCBs contained in marine sediments by the green macroalga Ulva rigida was investigated in both laboratory and field experiments. Under laboratory conditions, total PCBs (tPCBs) uptake was significantly greater in live vs dead plants. The concentration of tPCB taken up in live plants was greatest in the first 24h (1580μgkg−1 dry weight), and then increased at a lower rate from day 2 to 14. Dead plants had a significantly lower tPCB concentration after 24h (609μgkg−1 dry weight) and lower uptake rate through day 14. Lesser chlorinated PCB congeners (below 123) made up the majority of PCBs taken up. Congener composition in both laboratory and field experiments was correlated to congener logKow value and sediment content. Field experiments showed that Ulva plants could concentrate PCBs to 3.9mgkg−1 in 24h. Thus, U. rigida is capable of removing PCBs in sediments at a rapid rate.
Afficher plus [+] Moins [-]Isolation and characterization of a novel hydrocarbon-degrading bacterium Achromobacter sp. HZ01 from the crude oil-contaminated seawater at the Daya Bay, southern China
2014
Deng, Mao-Cheng | Li, Jing | Liang, Fu-Rui | Yi, Meisheng | Xu, Xiao-Ming | Yuan, Jian-Ping | Peng, Juan | Wu, Chou-Fei | Wang, Jiang-Hai
Microorganisms play an important role in the biodegradation of petroleum contaminants, which have attracted great concern due to their persistent toxicity and difficult biodegradation. In this paper, a novel hydrocarbon-degrading bacterium HZ01 was isolated from the crude oil-contaminated seawater at the Daya Bay, South China Sea, and identified as Achromobacter sp. Under the conditions of pH 7.0, NaCl 3% (w/v), temperature 28°C and rotary speed 150rpm, its degradability of the total n-alkanes reached up to 96.6% after 10days of incubation for the evaporated diesel oil. Furthermore, Achromobacter sp. HZ01 could effectively utilize polycyclic aromatic hydrocarbons (PAHs) as its sole carbon source, and could remove anthracene, phenanthrene and pyrence about 29.8%, 50.6% and 38.4% respectively after 30days of incubation. Therefore, Achromobacter sp. HZ01 may employed as an excellent degrader to develop one cost-effective and eco-friendly method for the bioremediation of marine environments polluted by crude oil.
Afficher plus [+] Moins [-]Assessing weathered Endicott oil biodegradation in brackish water
2014
Personna, Yves Robert | King, Thomas | Boufadel, Michel C. | Zhang, Shuangyi | Kustka, Adam
We evaluated the biodegradability of physically (WAF) and chemically (CEWAF) dispersed oil in brackish water (salinity ∼6.5g/L), and the influence of nutrient availability (low nutrient-LN: background water vs. high nutrient-HN: addition of 100mgNO3-N/L and 10mg PO4-P/L to background water) on oil biodegradation rates at 15±0.5°C for 42days. No oil removal occurred in WAF compared with CEWAF: 24% in HN and 14% in LN within two weeks. The oil biodegradation concerned mainly alkanes as confirmed by GC/MS analyses. Higher O2 consumption (10.30mgL−1day−1) and CO2 production (3.89mg CL−1day−1) were measured in HN compared with LN (O2: 2.79mgL−1day−1, CO2:0.18mg CL−1day−1). Estimated biomass of hydrocarbon degraders and heterotrophic bacteria was at least an order of magnitude larger in HN than in LN. Combining dispersants with nutrients could enhance oil biodegradation and help improve oil spill mitigation responses.
Afficher plus [+] Moins [-]Enhanced biodegradation of total polycyclic aromatic hydrocarbons (TPAHs) by marine halotolerant Achromobacter xylosoxidans using Triton X-100 and β-cyclodextrin – A microcosm approach
2014
Dave, Bharti P. | Ghevariya, Chirag M. | Bhatt, Jwalant K. | Dudhagara, Dushyant R. | Rajpara, Rahul K.
Ability of Achromobacter xylosoxidans, a chrysene degrading marine halotolerant bacterium to degrade polycyclic aromatic hydrocarbons (PAHs) using a cost effective laboratory microcosm approach, was investigated. Effect of variables as chrysene, glucose as a co-substrate, Triton X-100 as a non-ionic surfactant and β-cyclodextrin as a PAHs solubilizer was examined on degradation of low molecular weight (LMW) and high molecular weight (HMW) PAHs. A total of eleven PAHs detected from polluted saline soil were found to be degraded. Glucose, in combination with Triton X-100 and β-cyclodextrin resulted in 2.8 and 1.4-fold increase in degradation of LMW PAHs and 7.59 and 2.23-fold increase in degradation of HMW PAHs, respectively. Enhanced biodegradation of total PAHs (TPAHs) by amendments with Triton X-100 and β-cyclodextrin using Achromobacter xylosoxidans can prove to be promising approach for in situ bioremediation of marine sites contaminated with PAHs.
Afficher plus [+] Moins [-]Bioremediation and fodder potentials of two Sargassum spp. in coastal waters of Shenzhen, South China
2014
Yu, Zonghe | Zhu, Xiaoshan | Jiang, Lichang | Luo, Peng | Hu, Chaoqun
In this study, the bioremediation potentials of two seaweeds (Sargassum hemiphyllum and S. henslowianum) against pollution in a coastal mariculture area of Shenzhen, South China, were investigated by comparing the growth, nutrient bioaccumulation capacity of plants from the seaweed bed (control site) with plants from the fish farm. Results indicated that both species are potential candidates for bioremediation in the fish farm areas in terms of their high growth rates and high bioaccumulation capacities on inorganic nutrients. Both Sargassum spp. contain high levels of crude protein (11.7–14.0%) and crude fat (2.2–2.7%), suggesting high nutritional values. The S. hemiphyllum may serve as a good aquaculture fodder with high nutritional compositions and low heavy metal contents. However, heavy metals (Cr, Pb and Cd) of S. henslowianum exceed the maximum allowable concentrations as aquatic feed, which restricts its fodder application. In general, the results of this study may contribute to the marine pollution bioremediation in the coastal areas of South China, especially in mariculture zones.
Afficher plus [+] Moins [-]The application of a carrier-based bioremediation strategy for marine oil spills
2014
Sheppard, Petra J. | Simons, Keryn L. | Adetutu, Eric M. | Kadali, Krishna K. | Juhasz, Albert L. | Manefield, Mike | Sarma, Priyangshu M. | Lal, Banwari | Ball, A. S.
The application of recycled marine materials to develop sustainable remediation technologies in marine environment was assessed. The remediation strategy consisted of a shell carrier mounted bacterial consortium composed of hydrocarbonoclastic strains enriched with nutrients (Bioaug SC). Pilot scale studies (5000l) were used to examine the ability of Bioaug-SC to degrade weathered crude oil (10gl−1; initially 315,000±44,000mgl−1) and assess the impacts of the introduction and biodegradation of oil. Total petroleum hydrocarbon mass was effectively reduced by 53.3 (±5.75)% to 147,000 (±21,000) mgl−1 within 27weeks. 16S rDNA bacterial community profiling using Denaturant Gradient Gel Electrophoresis revealed that cyanobacteria and Proteobacteria dominated the microbial community. Aquatic toxicity assessment was conducted by ecotoxicity assays using brine shrimp hatchability, Microtox and Phaeodactylum tricornutum. This study revealed the importance of combining ecotoxicity assays with oil chemistry analysis to ensure safe remediation methods are developed.
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