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Effect of heavy metal-induced stress on two extremophilic microbial communities from Caviahue-Copahue, Argentina
2021
Massello, Francisco L. | Donati, Edgardo
Metal pollution is a great concern worldwide and the development of new technologies for more sustainable extraction methods as well as for the remediation of polluted sites is essential. Extremophilic microorganisms are attractive for this purpose since they have poly-resistance mechanisms which make them versatile. In this work, we sampled an acidic river and a hot spring of Caviahue-Copahue volcanic environment. The indigenous microbial communities were exposed to five heavy metals (Cd, Co, Cu, Ni and Zn) in batch-cultures favouring different metabolisms of biotechnological interest. Remarkably, high tolerance values were reached in all the cultures, even though most of the metals studied were not present in the environmental sample. Particularly, outstanding tolerances were exhibited by acidophiles, which grew at concentrations as high as 400 mM of Zn and Ni. High-throughput amplicon sequencing of 16S rRNA gene was used to study the indigenous communities and the resistant consortia. We took three approaches for the analysis: phylotypes, OTUs and amplicon sequence variants (ASVs). Interestingly, similar conclusions were drawn in all three cases. Analysing the phylogenetic structure and functional potential of the adapted consortia, we found that the strongest selection was exerted by the culture media. Notably, there was a poor correlation between alpha diversity and metal stress; furthermore, metal stress did not seem to harm the functional potential of the consortia. All these results reveal a great adaptability and versatility. At the end, 25 metal-resistant extremophilic consortia with potential uses in bioremediation, bioleaching or biomonitoring processes were obtained.
Afficher plus [+] Moins [-]Trace metals at the tree-litter-soil- interface in Brazilian Atlantic Forest plots surrounded by sources of air pollution
2021
Nakazato, Ricardo Keiichi | Lourenço, Isabela S. | Esposito, Marisia P. | Lima, Marcos E.L. | Ferreira, Mauricio L. | Campos, Rafaela de O.A. | Rinaldi, Mirian C.S. | Domingos, Marisa
Passive biomonitoring was applied in four Atlantic forest plots in southeast Brazil, affected by different levels of trace metal pollution (OP site located in Minas Gerais State and PEFI, PP and STG located in São Paulo State). Native tree species were selected as biomonitors according to their abundance in each plot and successional classification. Current trace metal concentrations in total suspended particles, leaves of non-pioneer (NPi) and pioneer (Pi) species, topsoil (0–20 cm) and litter and concentration ratios at the plant/soil interface were analyzed to verify the atmosphere-plant-soil interactions, basal concentrations, spatial variations and metal accumulation at the ecosystem level. Redundant analysis helped to identify similar characteristics of metal concentrations in PP and PEFI, which can be influenced by the high concentrations of elements related to anthropogenic inputs. Analysis of variance and multivariate statistics indicated that the trees of OP presented higher concentrations of Cr, Fe, Mn and Ni than those in the other sites. High enrichment of Cd, Fe, Ni in non-pioneer plants indicated that the PP forest (initially considered as the least polluted) has still been affected by metal pollution. Soil collected in STG was enriched by all elements, however these elements were low available for plant uptake. Metal deposited in leaves and litter was an important sink for soil cycling, nevertheless, these metals are not bioavailable in most cases. Non-pioneer tree species revealed to be more appropriate than pioneer species to indicate the current panorama of the contamination and bioavailability levels of trace metals in the tree community-litter-soil interface of the Atlantic forest remnants included in this study.
Afficher plus [+] Moins [-]Airborne particle accumulation and loss in pollution-tolerant lichens and its magnetic quantification
2021
Chaparro, Marcos A.E.
Pollution-tolerant lichens are recognized ecological indicators of air pollution in cities, which can also collect airborne anthropogenic particles in their tissues. Harmful (sub)micron-sized magnetites are a ubiquitous component of air particle pollution, adversely impacting human health. In this work, in situ magnetic susceptibility κᵢₛ of well-characterized ultrafine magnetite and lichen thalli were measured to quantify the amount of airborne magnetic particles (AMP) after calibration and to assess the lichen's decontamination over time. Up to 2850 magnetic measurements were carried out in twenty-nine transplanted lichens (collected in urban and clean areas) from winter 2020 to winter 2021. Before the transplants, their initial κᵢₛ values were 0.23–9.45 × 10⁻⁵ SI, representing AMP contents of 0.1–4.6 mg in lichen thalli. After lichens were transplanted to a shared site, the magnetic signals evidenced short-term increases and long-term decreases. After three, five and nine months, the AMP loss is more pronounced for transplanted lichens from polluted (e.g., AMP_5-months loss = 0.59 mg) than clean (= 0.08 mg) sites. Rainfall influenced the lichen's decontamination between seasons. In situ measurements and lichens offer a valued and time-saving methodology for biomonitoring harmful airborne particles simply and effectively.
Afficher plus [+] Moins [-]Untangling causes of variation in mercury concentration between flight feathers
2021
Gatt, Marie Claire | Furtado, Ricardo | Granadeiro, José Pedro | Lopes, Daniel | Pereira, Eduarda | Catry, Paulo
Bird feathers are one of the most widely used animal tissue in mercury biomonitoring, owing to the ease of collection and storage. They are also the principal excretory pathway of mercury in birds. However, limitations in our understanding of the physiology of mercury deposition in feathers has placed doubt on the interpretation of feather mercury concentratoins. Throughout the literature, moult sequence and the depletion of the body mercury pool have been taken to explain patterns such as the decrease in feather mercury from the innermost (P1) to the outermost primary feather (P10) of the wing. However, it has been suggested that this pattern is rather a measurement artefact as a result of the increased feather mass to length ratio along the primaries, resulting in a dilution effect in heavier feathers. Here, we attempt to untangle the causes of variation in feather mercury concentrations by quantifying the mercury concentration as μg of mercury (i) per gram of feather, (ii) per millimetre of feather, and (iii) per day of feather growth in the primary feathers of Bulwer’s Petrel Bulweria bulwerii chicks, effectively controlling for some of the axes of variation that may be acting in adults, and monitoring the growth rate of primary feathers in chicks. The mercury concentration in Bulwer’s Petrel chicks’ primaries increased from the innermost to the outermost primary for all three concentration measures, following the order of feather emergence. These observations confirm that the pattern of mercury concentration across primary feathers is not an artefact of the measure of concentration, but is likely an effect of the order of feather growth, whereby the earlier grown feathers are exposed to higher blood mercury concentrations than are later moulted feathers as a result of blood mercury depletion.
Afficher plus [+] Moins [-]Short-term variability of bisphenols in spot, morning void and 24-hour urine samples
2021
Gys, Celine | Bastiaensen, Michiel | Malarvannan, Govindan | Ait Bamai, Yu | Araki, Atsuko | Covaci, Adrian
Due to worldwide regulations on the application of the high production volume industrial chemical bisphenol A (BPA) in various consumer products, alternative bisphenols such as bisphenol F (BPF) and bisphenol S (BPS) are increasingly used. To assess human exposure to these chemicals, biomonitoring of urinary concentrations is frequently used. However, the short-term variability of alternative bisphenols has not been evaluated thoroughly yet, which is essential to achieve a correct estimation of exposure. In this study, we collected all spot urine samples from ten healthy adults for five consecutive days, and an additional 24 h pooled sample. We measured the concentrations of seven bisphenols (BPAF, BPF, BPA, BPB, BPZ, BPS and BPAP) in these samples using gas chromatography coupled to tandem mass spectrometry. BPA, BPF and BPS were frequently found in spot samples (>80%), while bisphenol AP (BPAP) was detected in 43% of spot samples. Calculations of intra-class correlation coefficients (ICCs) showed that reproducibility of these four bisphenols was relatively poor (<0.01–0.200) but improved when concentrations were corrected for urine dilution using creatinine levels (0.128–0.401). Of these four bisphenols, BPF showed the best reproducibility (ICC 0.200–0.439) and BPS the most variability (ICC <0.01–0.128). In general, the within-participant variability of bisphenol levels was the largest contributor to the total variance (47–100%). We compared repeated first morning voids to 24 h pooled urine and found no significantly different concentrations for BPA, BPF, BPS, or BPAP. Levels of BPA and BPF differed significantly depending on the sampling time throughout the day. The findings in this study suggest that collecting multiple samples per participant over a few days, in predefined time windows throughout the day, could result in a more reliable estimation of internal exposure to bisphenols.
Afficher plus [+] Moins [-]Metal accumulation varies with life history, size, and development of larval amphibians
2021
Smalling, Kelly L. | Oja, Emily B. | Cleveland, Danielle M. | Davenport, Jon M. | Eagles-Smith, Collin | Campbell Grant, Evan H. | Kleeman, Patrick M. | Halstead, Brian J. | Stemp, Kenzi M. | Tornabene, Brian J. | Bunnell, Zachary J. | Hossack, Blake R.
Amphibian larvae are commonly used as indicators of aquatic ecosystem health because they are susceptible to contaminants. However, there is limited information on how species characteristics and trophic position influence contaminant loads in larval amphibians. Importantly, there remains a need to understand whether grazers (frogs and toads [anurans]) and predators (salamanders) provide comparable information on contaminant accumulation or if they are each indicative of unique environmental processes and risks. To better understand the role of trophic position in contaminant accumulation, we analyzed composite tissues for 10 metals from larvae of multiple co-occurring anuran and salamander species from 20 wetlands across the United States. We examined how metal concentrations varied with body size (anurans and salamanders) and developmental stage (anurans) and how the digestive tract (gut) influenced observed metal concentrations. Across all wetlands, metal concentrations were greater in anurans than salamanders for all metals tested except mercury (Hg), selenium (Se), and zinc (Zn). Concentrations of individual metals in anurans decreased with increasing weight and developmental stage. In salamanders, metal concentrations were less correlated with weight, indicating diet played a role in contaminant accumulation. Based on batches of similarly sized whole-body larvae compared to larvae with their digestive tracts removed, our results indicated that tissue type strongly affected perceived concentrations, especially for anurans (gut represented an estimated 46–97% of all metals except Se and Zn). This suggests the reliability of results based on whole-body sampling could be biased by metal, larval size, and development. Overall, our data shows that metal concentrations differs between anurans and salamanders, which suggests that metal accumulation is unique to feeding behavior and potentially trophic position. To truly characterize exposure risk in wetlands, species of different life histories, sizes and developmental stages should be included in biomonitoring efforts.
Afficher plus [+] Moins [-]Urinary phthalate metabolites among workers in plastic greenhouses in western China
2021
Zhang, Yanxia | Huang, Biao | He, Huan | Wang, Xinkai | Sabel, Clive E. | Thomsen, Marianne | Chen, Zhikun | Wang, Weixi
Agricultural plastic greenhouse (PG) production can extend the growing season of crops to satisfy domestic consumption in countries such as China. Workers in PGs have potential higher phthalate exposure risks than the general population as phthalate accumulation has been observed in greenhouse soil, air, and crops. To date, biomonitoring tests of phthalates for the working population have not been carried out. To address this shortage, we conducted a pilot study in Shaanxi Province, China, among 35 healthy PG workers by follow-up recording their seasonal dietary habits and work activities and urine sample collection and measurement between 2018 and 2019. The objectives were to uncover the association between phthalate metabolites and the population characteristics, seasonal and diurnal variations and causes, and to estimate exposure risks and contributions of exposure pathways from PG production systems. A total of 13 phthalate metabolite concentrations (Σ₁₃ phthalate metabolites) ranged from 102 to 781 (5th-95th) ng/mL (median: 300 ng/mL). Mono-n-butyl phthalate (MNBP) made up 51.3% of Σ₁₃ phthalate metabolites, followed by the sum of four di-2-ethylhexyl phthalate (DEHP) metabolites (24.2%), mono-2-isobutyl phthalate (MIBP) (13.4%), and mono-ethyl phthalate (MEP) (9.8%). The concentrations of MNBP and MIBP in summer were significantly higher than the levels in winter (p < 0.0001). A total of 62.3% of the PG worker population was shown to have exposure risks, and the proportion was as high as 79.4% in summer. Phthalate exposure of the workers from PG production systems constituted over 20% of the total creatinine-based daily intake, and consuming vegetables and fruit planted in PGs and inhalation in PGs were the two largest exposure pathways. Our findings demonstrate that it is important to protect workers in PGs from phthalate exposure risks, and phasing out the use of plastic materials containing phthalates in PGs is imperative, to guarantee food safety in PGs.
Afficher plus [+] Moins [-]Background concentrations of trace metals As, Ba, Cd, Co, Cu, Ni, Pb, Se, and Zn in 214 Florida urban soils: Different cities and land uses
2020
da Silva, Evandro B. | Gao, Peng | Xu, Min | Guan, Dongxing | Tang, Xianjin | Ma, Lena Q.
Soil contamination in urban environment by trace metals is of public concerns. For better risk assessment, it is important to determine their background concentrations in urban soils. For this study, we determined the background concentrations of 9 trace metals including As, Ba, Cd, Co, Cu, Ni, Pb, Se, and Zn in 214 urban soils in Florida from two large cities (Orlando and Tampa) and 4 small cities (Clay County, Ocala, Pensacola and West Palm Beach). The objectives were to determine: 1) total concentrations of trace metals in urban soils in cities of different size; 2) compare background concentrations to Florida Soil Cleanup Target Levels (FSCTLs); and 3) determine their distribution and variability in urban soils via multivariate statistical analysis. Elemental concentrations in urban soils were variable, with Pb being the highest in 5 cities (165–552 mg kg⁻¹) and Zn being the highest concentration in Tampa (1,000 mg kg⁻¹). Besides, the As and Pb concentrations in some soils exceeded the FSCTL for residential sites at 2.1 mg kg⁻¹ As and 400 mg kg⁻¹ Pb. Among the cities, Clay County and Orlando had the lowest concentrations for most elements, with Cd, Co, and As being the lowest while Ba, Pb and Zn being the highest. Among all values, geometric means were the lowest while 95th percentile was the highest for all metals. Most 95th percentile values were 2–3 folds higher than the GM data, with Pb presenting the greatest difference, being 4 times greater than GM value (58.9 vs. 13.6 mg kg⁻¹). Still they were lower than FSCTL, with As exceeding FSCTL for residential sites at 2.1 mg kg⁻¹. In addition, the linear discriminate analysis showed distinct separation among the cities: Ocala (Ba & Ni) and Pensacola (As & Pb) were distinctly different from each other and from other cities with higher metal concentrations. The large variations among elemental concentrations showed the importance to establish proper background concentrations of trace metals in urban soils.
Afficher plus [+] Moins [-]High inter-species differences of 12378-polychlorinated dibenzo-p-dioxin between humans and mice
2020
Dong, Zhaomin | Ben, Yujie | Li, Yu | Li, Tong | Wan, Yi | Hu, Jianying
Although huge interspecies differences in the response to dioxins have been acknowledged, toxic equivalency factors derived from rodent studies are often used to assess human health risk. To determine interspecies differences, we first developed a toxicokinetic model in humans by measuring dioxin concentrations in environmental and biomonitoring samples from Southern China. Significant positive correlations between dioxin concentrations in blood and age were observed for seven dioxin congeners, indicating an age-dependent elimination rate. Based on toxicokinetic models in humans, the half-lives of 15 dioxin congeners were estimated to be 1.60–28.55 years. In consideration that the highest contribution to total toxic equivalency in blood samples was by 12378-polychlorinated dibenzo-p-dioxin (P₅CDD), this study developed a physiologically based pharmacokinetic (PBPK) model of 12378-P₅CDD levels in the liver, kidney, and fat of C57/6J mice exposed to a single oral dose, and the half-life was estimated to be 26.1 days. Based on estimated half-lives in humans and mice, we determined that the interspecies difference of 12378-P₅CDD was 71, much higher than the default usually used in risk assessment. These results could reduce the uncertainty human risk assessment of 12378-P₅CDD, and our approach could be used to estimate the interspecies differences of other dioxin congeners.
Afficher plus [+] Moins [-]Environmental DNA metabarcoding reveals estuarine benthic community response to nutrient enrichment – Evidence from an in-situ experiment
2020
Clark, D.E. | Pilditch, C.A. | Pearman, J.K. | Ellis, J.I. | Zaiko, A.
Nutrient loading is a major threat to estuaries and coastal environments worldwide, therefore, it is critical that we have good monitoring tools to detect early signs of degradation in these ecologically important and vulnerable ecosystems. Traditionally, bottom-dwelling macroinvertebrates have been used for ecological health assessment but recent advances in environmental genomics mean we can now characterize less visible forms of biodiversity, offering a more holistic view of the ecosystem and potentially providing early warning signals of disturbance. We carried out a manipulative nutrient enrichment experiment (0, 150 and 600 g N fertilizer m⁻²) in two estuaries in New Zealand to assess the effects of nutrient loading on benthic communities. After seven months of enrichment, environmental DNA (eDNA) metabarcoding was used to examine the response of eukaryotic (18S rRNA), diatom only (rbcL) and bacterial (16S rRNA) communities. Multivariate analyses demonstrated changes in eukaryotic, diatom and bacterial communities in response to nutrient enrichment at both sites, despite differing environmental conditions. These patterns aligned with changes in macrofaunal communities identified using traditional morphological techniques, confirming concordance between disturbance indicators detected by eDNA and current monitoring approaches. Clear shifts in eukaryotic and bacterial indicator taxa were seen in response to nutrient loading while changes in diatom only communities were more subtle. Community changes were discernible between 0 and 150 g N m⁻² treatments, suggesting that estuary health assessment tools could be developed to detect early signs of degradation. Increasing variation in community structure associated with nutrient loading could also be used as an indicator of stress or approaching tipping points. This work represents a first step towards the development of molecular-based estuary monitoring tools, which could provide a more holistic and standardized approach to ecosystem health assessment with faster turn-around times and lower costs.
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