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Integrated multi-biomarker responses in two dreissenid species following metal and thermal cross-stress
2016
Potet, Marine | Devin, Simon | Pain-Devin, Sandrine | Rousselle, Philippe | Giambérini, Laure
With current global changes, the combination of several stressors such as temperature and contaminants may impact species distribution and ecosystem functioning. In this study, we evaluated the combined impact of two metals (Ni and Cr) with a thermal stress (from 12 to 17 °C) on biomarker responses in two bivalves, Dreissena rostriformis bugensis and Dreissena polymorpha. Biomarkers are informative tools to evaluate exposure and effects of stressors on organisms. The set of 14 biomarkers measured here was representative of both physiologic (filtration activity) and cellular antioxidant and detoxification mechanisms. Our aim was to study the response pattern of both species, and its meaning in terms of invasive potential. The implications for the use of these mussels in environmental monitoring are also discussed. Results evidenced that the two species do not respond to multiple stressors in the same way. Indeed, the effects of contamination on biomarker responses were more marked for D. polymorpha, especially under nickel exposure. While we cannot conclude as to the effect of temperature, invasiveness could be influenced by species sensitivity to contaminants. The physiological and cellular differences between D. polymorpha and D. r. bugensis might also be of concern for environmental risk assessment. The two species present differential bioaccumulation patterns, filtration activity and cellular biomarker responses. If D. polymorpha populations decline, their substitution by D. r. bugensis for biomonitoring or laboratory studies will not be possible without a deeper understanding of biomarker responses of the new invasive.
Afficher plus [+] Moins [-]Size-dependent depletion and community disturbance of phytoplankton under intensive oyster mariculture based on HPLC pigment analysis in Daya Bay, South China Sea
2016
Jiang, Tao | Chen, Feiyu | Yu, Zonghe | Lü, Lin | Wang, Zhaohui
In this study, we conducted a 14-month investigation in Daya Bay, southern China to understand the effects of oyster farming on phytoplankton community and biomass by using size-fractionated phytopigments. Results proved the filtering effects of oysters on phytoplankton biomass. During the oyster culture period, the average concentration of total chlorophyll a (sum of size-fractionated Chl a) within the farming area was approximately 60% lower than that at the reference site. Phytoplankton depletion in the aquaculture zone mainly occurred in micro-sized fractions (>20 μm) of Chl a, fucoxanthin, and peridinin. The influence of oyster filtration on nano-sized (2.7–20 μm) pigments seemed less than that on micro-sized ones. The depletion of peridinin and 19′-hex-fucoxanthin in aquaculture zone was higher than those of the other pigments, which indicated that flagellated cells might be selectively filtered by oysters and could be more easily influenced by oyster aquaculture. The pico-sized Chl a (<2.7 μm) comprised 24% of total Chl a on the average in the aquaculture zone during the cultural period compared to 6% in the reference site. Picoeukaryote abundance, which was determined via flow cytometry, was significantly higher in the aquaculture zone than in the non-aquaculture areas. The abundance of picoeukaryote is significantly and positively correlated with the concentrations of pico-sized prasinoxanthin, violaxanthin, and neoxanthin, indicating that picoeukaryote is dominated by those in prasinophyte. The results suggest that oyster aquaculture might stimulate the growth of prasinophyte, although the seasonal variations are mainly controlled by the water temperature in the study area. This research highlights the successful use of size-fractionated phytopigments to estimate size-specific phytoplankton biomass and community, which can be applied as a routine method to monitor the environmental effect and food resources of bivalve aquaculture.
Afficher plus [+] Moins [-]Development of a filter-based method for detecting silver nanoparticles and their heteroaggregation in aqueous environments by surface-enhanced Raman spectroscopy
2016
The rising application of silver nanoparticles (AgNPs) and subsequent release into aquatic systems have generated public concerns over their potential risk and harm to aquatic organisms and human health. Effective and practical analytical methods for AgNPs are urgently needed for their risk assessment. In this study we established an innovative approach to detect trace levels of AgNPs in environmental water through integrating a filtration technique into surface-enhanced Raman spectroscopy (SERS) and compared it with previously established centrifuge-based method. The purpose of filtration was to trap and enrich salt-aggregated AgNPs from water samples onto the filter membrane, through which indicator was then passed and complexed with AgNPs. The enhanced SERS signals of indicator could reflect the presence and quantity of AgNPs in the samples. The most favorable benefit of filtration is being able to process large volume samples, which is more practical for water samples, and greatly improves the sensitivity of AgNP detection. In this study, we tested 20 mL AgNPs-containing samples and the filter-based method is able to detect AgNPs as low as 5 μg/L, which is 20 folds lower than the centrifuge-based method. In addition, the speed and precision of the detection were greatly improved. This approach was used to detect trace levels of AgNPs in real environmental water successfully. Meanwhile, the heteroaggregation of AgNPs with minerals in water was reliably monitored by the new method. Overall, a combination of the filtration-SERS approach provides a rapid, simple, and sensitive way to detect AgNPs and analyze their environmental behavior.
Afficher plus [+] Moins [-]Investigation and risk evaluation of the occurrence of carbamazepine, oxcarbazepine, their human metabolites and transformation products in the urban water cycle
2016
Brezina, Elena | Prasse, Carsten | Meyer, Johannes | Mückter, Harald | Ternes, Thomas A.
Trace organic contaminants such as pharmaceuticals, personal care products and industrial chemicals are frequently detected in the urban water cycle, including wastewater, surface water and groundwater, as well as drinking water. These also include human metabolites (HMs), which are formed in the human body and then excreted via urine or feces, as well as transformation products (TPs) formed in engineered treatment systems and the aquatic environment. In the current study, the occurrence of HMs as well as their TPs of the anticonvulsants carbamazepine (CBZ) and oxcarbazepine (OXC) were investigated using LC tandem MS in effluents of wastewater treatment plants (WWTPs), surface water and groundwater. Highest concentrations were observed in raw wastewater for 10,11-dihydro-10,11-dihydroxycarbamazepine (DiOHCBZ), 10,11-dihydro-10-hydroxy-cabamazepine (10OHCBZ) and CBZ with concentrations ranging up to 2.7 ± 0.4, 1.7 ± 0.2 and 1.07 ± 0.06 μg L−1, respectively. Predictions of different toxicity endpoints using a Distributed Structure-Searchable Toxicity (DSSTox) expert system query indicated that several HMs and TPs, in particular 9-carboxy-acridine (9-CA-ADIN) and acridone (ADON), may exhibit an increased genotoxicity compared to the parent compound CBZ. As 9-CA-ADIN was also detected in groundwater, a detailed investigation of the genotoxicity of 9-CA-ADIN is warranted. Investigations of an advanced wastewater treatment plant further revealed that the discharge of the investigated compounds into the aquatic environment could be substantially reduced by ozonation followed by granular activated carbon (GAC) filtration.
Afficher plus [+] Moins [-]Exposure of marine mussels Mytilus spp. to polystyrene microplastics: Toxicity and influence on fluoranthene bioaccumulation
2016
Paul Pont, Ika | Lacroix, Camille | González Fernández, Carmen | Hégaret, Hélène | Lambert, Christophe | Le Goïc, Nelly | Frère, Laura | Cassone, Anne-Laure | Sussarellu, Rossana | Fabioux, Caroline | Guyomarch, Julien | Albentosa, Marina | Huvet, Arnaud | Soudant, Philippe
The effects of polystyrene microbeads (micro-PS; mix of 2 and 6 μm; final concentration: 32 μg L−1) alone or in combination with fluoranthene (30 μg L−1) on marine mussels Mytilus spp. were investigated after 7 days of exposure and 7 days of depuration under controlled laboratory conditions. Overall, fluoranthene was mostly associated to algae Chaetoceros muelleri (partition coefficient Log Kp = 4.8) used as a food source for mussels during the experiment. When micro-PS were added in the system, a fraction of FLU transferred from the algae to the microbeads as suggested by the higher partition coefficient of micro-PS (Log Kp = 6.6), which confirmed a high affinity of fluoranthene for polystyrene microparticles. However, this did not lead to a modification of fluoranthene bioaccumulation in exposed individuals, suggesting that micro-PS had a minor role in transferring fluoranthene to mussels tissues in comparison with waterborne and foodborne exposures. After depuration, a higher fluoranthene concentration was detected in mussels exposed to micro-PS and fluoranthene, as compared to mussels exposed to fluoranthene alone. This may be related to direct effect of micro-PS on detoxification mechanisms, as suggested by a down regulation of a P-glycoprotein involved in pollutant excretion, but other factors such as an impairment of the filtration activity or presence of remaining beads in the gut cannot be excluded. Micro-PS alone led to an increase in hemocyte mortality and triggered substantial modulation of cellular oxidative balance: increase in reactive oxygen species production in hemocytes and enhancement of anti-oxidant and glutathione-related enzymes in mussel tissues. Highest histopathological damages and levels of anti-oxidant markers were observed in mussels exposed to micro-PS together with fluoranthene. Overall these results suggest that under the experimental conditions of our study micro-PS led to direct toxic effects at tissue, cellular and molecular levels, and modulated fluoranthene kinetics and toxicity in marine mussels.
Afficher plus [+] Moins [-]Microplastic pollution is widely detected in US municipal wastewater treatment plant effluent
2016
Mason, Sherri A. | Garneau, Danielle | Sutton, Rebecca | Chu, Yvonne | Ehmann, Karyn | Barnes, Jason | Fink, Parker | Papazissimos, Daniel | Rogers, Darrin L.
Municipal wastewater effluent has been proposed as one pathway for microplastics to enter the aquatic environment. Here we present a broad study of municipal wastewater treatment plant effluent as a pathway for microplastic pollution to enter receiving waters. A total of 90 samples were analyzed from 17 different facilities across the United States. Averaging all facilities and sampling dates, 0.05 ± 0.024 microparticles were found per liter of effluent. Though a small value on a per liter basis, even minor municipal wastewater treatment facilities process millions of liters of wastewater each day, yielding daily discharges that ranged from ∼50,000 up to nearly 15 million particles. Averaging across the 17 facilities tested, our results indicate that wastewater treatment facilities are releasing over 4 million microparticles per facility per day. Fibers and fragments were found to be the most common type of particle within the effluent; however, some fibers may be derived from non-plastic sources. Considerable inter- and intra-facility variation in discharge concentrations, as well as the relative proportions of particle types, was observed. Statistical analysis suggested facilities serving larger populations discharged more particles. Results did not suggest tertiary filtration treatments were an effective means of reducing discharge. Assuming that fragments and pellets found in the effluent arise from the ‘microbeads’ found in many cosmetics and personal care products, it is estimated that between 3 and 23 billion (with an average of 13 billion) of these microplastic particles are being released into US waterways every day via municipal wastewater. This estimate can be used to evaluate the contribution of microbeads to microplastic pollution relative to other sources (e.g., plastic litter and debris) and pathways (e.g., stormwater) of discharge.
Afficher plus [+] Moins [-]Pretreatment of Petrochemical Secondary Effluent by Micro-flocculation and Dynasand Filtration: Performance and DOM Removal Characteristics
2016
Wu, Changyong | Wang, Yaning | Zhou, Baoying | Zhu, Chen
A pilot scale micro-flocculation and dynasand filtration process was used to pretreat the petrochemical secondary effluent. The suspended solids (SS) and the dissolved organic matter (DOM) removal characteristics were investigated. The results showed that the optimized poly aluminum ferric chloride (PFAC) dosage was 10 mg/L during the experiment. In this dosage period, the SS removal rate was as high as 50.58 % with the effluent SS of 15.38 mg/L when the influent SS was 33.53 mg/L. The COD removal rate was 10.42 %. The DOM fraction with large apparent molecular weight (MW) higher than 3 k was removed more significantly than that of small molecular DOM. Resin fractionation showed that the micro-flocculation and dynasand filtration process could preferentially remove the hydrophobic neutrals (HON) and hydrophobic acids (HOA) of DOM, so it could be suitable as the pretreatment unit. When oxidized by catalytic ozonation, the ozone consumption of COD removal for filtrated effluent was 1.2 g-O₃/g-COD while it was 1.6 g-O₃/g-COD for untreated petrochemical secondary effluent, saving 25 % of ozone consumption. The micro-flocculation and dynasand filtration is a suitable pretreatment process for petrochemical secondary effluent, especially when the subsequent unit is the catalytic ozonation process.
Afficher plus [+] Moins [-]Hydraulic Performance and Pollutant Concentration Profile in a Stormwater Runoff Filtration Systems
2016
Haile, Tadele Measho | Hobiger, Gerhard | Kammerer, Gerhard | Allabashi, Roza | Schaerfinger, Birgit | Fuerhacker, Maria
Stormwater filtration system has proven to be effective for the removal of dissolved and particulate pollutants from roadways and car parking areas. However, the long-term treatment performance of filtration systems strongly depends on the hydraulic conductivity and sorption capacity of the filter media. This paper sought to provide information regarding the hydraulic performance, characteristics and metal concentration profiles in sediments accumulated at the surface of filtration systems (SDPL) and core filter media (FMC). The lifespan of the filter media was used to estimate the lifespan of the filter media. The results showed that saturated hydraulic conductivity of the filtration systems have significantly reduced over the operational time, yet acceptable (Kf = 5.9 × 10⁻⁵ to 1.4 × 10⁻⁴ m/s). The accumulated sediments (SDPL) were predominantly composed of fine particles with 70 % < 63 μm but the heavy metals were rather uniformly distributed in the different size fractions. The concentrations of heavy metals, particularly Cu, Pb and Zn were significantly higher in the SDPL and decreased with depth of the filter bed. However, Cr and Ni increased with depth of filter media demonstrating their removal was mainly by adsorption. Concentrations of Ba, Mn, Ti and V were comparable to Zn levels indicating comparable concentrations in roadway runoff. Simultaneous adsorption of multiple heavy metals in a column experiment demonstrated that the filter media could remain operational for over 34 years. However, there is a significant concern about their lifespan, particularly due to significant reduction in the hydraulic performance and the possibility of clogging of the systems over time. Therefore, to minimize hydraulic failure, the accumulated sediment be scraped off every 7 years.
Afficher plus [+] Moins [-]Seasonal Variability in Stormwater Quality Treatment of Permeable Pavements Situated Over Heavy Clay and in a Cold Climate
2016
Winston, Ryan J. | Davidson-Bennett, Keely M. | Buccier, Kristen M. | Hunt, W. F. (William Frederick)
Permeable pavements mitigate the impacts of urbanization on surface waters through pollutant load reduction, both by sequestration of pollutants and stormwater volume reduction through exfiltration. This study examined the non-winter water quality performance of two side-by-side permeable pavements in the Ohio snowbelt. The permeable interlocking concrete pavements were designed to drain impervious catchments 2.2 (large) and 7.2 (small) times larger than their surface area, were located over clay soils, and incorporated the internal water storage design feature. Nutrient reduction was similar to past studies—organic nitrogen and particulate phosphorus were removed through filtration and settling, while dissolved constituents received little treatment. Because of 16 and 32 % volume reductions in the small and large installations, respectively, nutrient loads were often significantly reduced but generally by less than 50 %. Aluminum, calcium, iron, magnesium, lead, chloride, and total suspended solids (TSS) concentrations and loads often increased after passing through the permeable pavements; effluent TSS loads were three- to five-fold higher than influent TSS loads. This was apparently due to seasonal release of clay- and silt-sized particles from the soils underlying the permeable pavement and inversely related to elapsed time since winter. The application of de-icing salt is thought to have caused deflocculation of the underlying soils, allowing particulates to exit with stormwater as it discharged from the underdrain of the permeable pavements. By autumn, both permeable pavements discharged metals and TSS concentrations similar to others in the literature, suggesting the de-icing effects lasted 3–6 months post-winter. Sodium may substantially affect the performance of permeable pavements following winter de-icing salt application, particularly when 2:1 clay minerals, such as vermiculites and smectites, predominate.
Afficher plus [+] Moins [-]Simulation of Terrigenous Minerogenic Particle Populations in Time and Space in Cayuga Lake, New York, in Response to Runoff Events
2016
Gelda, Rakesh K. | Effler, Steven W. | Prestigiacomo, Anthony R. | Peng, Feng | Watkins, James M. | Chapra, Steven
The important effects of minerogenic particles delivered from watersheds on optical and phosphorus metrics of lacustrine water quality have recently been quantified through measurements of the projected area of these particles per unit volume of water (PAVₘ), using an individual particle analysis technique. A mass balance type model for PAVₘ, partitioned according to the contributions of four size classes, is developed and tested for Cayuga Lake, New York, supported by long-term monitoring of PAVₘ in the lake and its primary tributaries. The model represents the source of PAVₘ of tributary inputs and three in-lake loss processes: (1) size-dependent settling, (2) enhancement of settling through aggregation, and (3) filter feeding by dreissenid mussels. The central roles of major runoff events and localized external loads of minerogenic sediment at one end of the lake in driving patterns of PAVₘ in time and space are successfully simulated, including (1) the higher PAVₘ levels in a shallow area (“shelf”) adjoining these inputs, relative to pelagic waters, following runoff events; and (2) the positive dependence of the shelf increases on the magnitude of the event. Analyses conducted with the model establish that settling, with aggregation enhancement, dominates the loss of PAVₘ from the water column of the shelf, while mussel filtration increases in relative importance in pelagic waters. The utility of PAVₘ predictions to quantify the effects of these particles on optical and phosphorus concentration metrics of water quality is established.
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