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Understanding aquaporin regulation defining silicon uptake and role in arsenic, antimony and germanium stress in pigeonpea (Cajanus cajan)
2022
Mandlik, Rushil | Singla, Pankaj | Kumawat, Surbhi | Khatri, Praveen | Ansari, Waquar | Singh, Anuradha | Sharma, Yogesh | Singh, Archana | Solanke, Amol | Nadaf, Altafhusain | Sonah, Humira | Deshmukh, Rupesh
Understanding of aquaporins (AQPs) facilitating the transport of water and many other small solutes including metalloids like silicon (Si) and arsenic (As) is important to develop stress tolerant cultivars. In the present study, 40 AQPs were identified in the genome of pigeonpea (Cajanus cajan), a pulse crop widely grown in semi-arid region and areas known to affected with heavy metals like As. Conserved domains, variation at NPA motifs, aromatic/arginine (ar/R) selectivity filters, and pore morphology defined here will be crucial in predicting solute specificity of pigeonpea AQPs. The study identified CcNIP2-1 as an AQP predicted to transporter Si (beneficial element) as well as As (hazardous element). Further Si quantification in different tissues showed about 1.66% Si in leaves which confirmed the predictions. Furthermore, scanning electron microscopy showed a higher level of Si accumulation in trichomes on the leaf surface. A significant alleviation in level of As, Sb and Ge stress was also observed when these heavy metals were supplemented with Si. Estimation of relative water content, H₂O₂, lipid peroxidation, proline, total chlorophyll content and other physiological parameters suggested Si derived stress tolerance. Extensive transcriptome profiling under different developmental stages from germination to senescence was performed to understand the tissue-specific regulation of different AQPs. For instance, high expression of TIP3s was observed only in reproductive tissues. Co-expression network developed using transcriptome data from 30 different conditions and tissues, showed interdependency of AQPs. Expression profiling of pigeonpea performed using real time PCR showed differential expression of AQPs after Si supplementation. The information generated about the phylogeny, distribution, molecular evolution, solute specificity, and gene expression dynamics in article will be helpful to better understand the AQP transport system in pigeonpea and other legumes.
Afficher plus [+] Moins [-]Selected technology-critical elements as indicators of anthropogenic groundwater contamination
2021
Amiel, Nitai | Dror, Ishai | Zurieli, Arik | Livshitz, Yakov | Reshef, Guy | Berkowitz, Brian
Groundwater contamination originating from anthropogenic industrial activities is a global concern, adversely impacting health of living organisms and affecting natural ecosystems. Monitoring contamination in a complex groundwater system is often limited by sparse data and poor hydrogeological delineation, so that numerous indicators (organic, inorganic, isotopic) are frequently used simultaneously to reduce uncertainty. We suggest that selected Technology-Critical Elements (TCEs), which are usually found in very low concentrations in the groundwater environment, might serve as contamination indicators that can be monitored through aquifer systems. Here, we demonstrate the use of selected TCEs (in particular, Y, Rh, Tl, Ga, and Ge) as indicators for monitoring anthropogenic groundwater contamination in two different groundwater systems, near the Dead Sea, Israel. Using these TCEs, we show that the sources of local groundwater contamination are phosphogypsum ponds located adjacent to fertilizer plants in two industrial areas. In addition, we monitored the spatial distribution of the contaminant plume to determine the extent of well and spring contamination in the region. Results show significant contamination of the groundwater beneath both fertilizer plants, leading to contamination of a series of wells and two natural springs. The water in these springs contains elevated concentrations of toxic metals; U and Tl levels, among others, are above the maximum concentration limits for drinking water.
Afficher plus [+] Moins [-]Vertical distribution and temporal dynamics of dissolved 137Cs concentrations in soil water after the Fukushima Dai-ichi Nuclear Power Plant accident
2017
Iwagami, Sho | Onda, Yūichi | Tsujimura, Maki | Hada, Manami | Pun, Ishwar
Radiocesium (137Cs) migration from headwater forested areas to downstream rivers has been investigated in many studies since the Fukushima Dai-ichi Nuclear Power Plant (FDNPP) accident, which was triggered by a catastrophic earthquake and tsunami on 11 March 2011. The accident resulted in the release of a huge amount of radioactivity and its subsequent deposition in the environment. A large part of the radiocesium released has been shown to remain in the forest. The dissolved 137Cs concentration and its temporal dynamics in river water, stream water, and groundwater have been reported, but reports of dissolved 137Cs concentration in soil water remain sparse.In this study, soil water was sampled, and the dissolved 137Cs concentrations were measured at five locations with different land-use types (mature/young cedar forest, broadleaf forest, meadow land, and pasture land) in Yamakiya District, located 35 km northwest of FDNPP from July 2011 to October 2012. Soil water samples were collected by suction lysimeters installed at three different depths at each site. Dissolved 137Cs concentrations were analyzed using a germanium gamma ray detector. The dissolved 137Cs concentrations in soil water were high, with a maximum value of 2.5 Bq/L in July 2011, and declined to less than 0.32 Bq/L by 2012. The declining trend of dissolved 137Cs concentrations in soil water was fitted to a two-component exponential model. The rate of decline in dissolved 137Cs concentrations in soil water (k1) showed a good correlation with the radiocesium interception potential (RIP) of topsoil (0–5 cm) at the same site. Accounting for the difference of 137Cs deposition density, we found that normalized dissolved 137Cs concentrations of soil water in forest (mature/young cedar forest and broadleaf forest) were higher than those in grassland (meadow land and pasture land).
Afficher plus [+] Moins [-]Bourgeoning impact of the technology critical elements in the marine environment
2020
Bu-Olayan, A.H. | Thomas, B.V.
Contamination of the Technology Critical Elements (TCE) through e-wastes and beach plastic wastes are some of the attributes to the recent rise in marine pollution. A generalized study of pollutants in the marine waters showed no evidence of the effect of TCE. However, an in-depth study revealed the mean TCE concentrations in the sequence of gallium (Ga) > thallium (Tl) > niobium (Nb) > tellurium (Te) > tantalum (Ta) > germanium (Ge) > indium (In) in wastewater (0.38 ng.L⁻¹) >sediment (0.3 ng g⁻¹) e-wastes (0.29 ng g⁻¹) > coastal water (0.26 ng.L⁻¹) > plastic wastes (0.133 ng g⁻¹) >fish (0.13 ng g⁻¹). The mean site-wise analysis of all the samples showed high TCE during winter than in the summer seasons as well, in the sequence of Site-II>Site-I>Site-V>Site-IV>Site-III. The mean distribution coefficient (Kd) of TCE was high in the summer (1.95) than during the winter (1.60) seasons but, the reverse seasonal effects were observed with the bioavailability (%BA) and geo-accumulation index (Igₑₒ). This index quantified TCE in e-wastes and plastic materials. Furthermore, these indicators labeled TCE as one among the sources for ‘Fish Kill,’ a futuristic threat to seafood consumers and a biomonitoring tool to marine pollution.
Afficher plus [+] Moins [-]Determination of radioactivity levels and heavy metal concentrations in seawater, sediment and anchovy (Engraulis encrasicolus) from the Black Sea in Rize, Turkey
2017
Baltas, Hasan | Kiris, Erkan | Sirin, Murat
Seawater, sediment and fish (anchovy) samples consumed in the Rize province of the Eastern Black Sea region of Turkey were collected from five different stations. The radioactivity levels (226Ra, 232Th, 40K and 137Cs) were determined in all the samples using a high-purity germanium detector. While 226Ra, 232Th and 40K radionuclides were detected in all samples, the radionuclide concentration of 137Cs, except for the sediment samples (mean activity is 9±1.4Bqkg−1), was not detected for the seawater and fish samples. The total annual effective dose rates from the ingestion of these radionuclides for fish were calculated using the measured activity concentrations in radionuclides and their ingested dose conversion factor. Also, the concentrations of some heavy metals in all the samples were determined. The activity and heavy metal concentration values that were determined for the seawater, sediment and fish samples were compared among the locations themselves and with literature values.
Afficher plus [+] Moins [-]Distribution of natural and anthropogenic radionuclides and associated radiation indices in the Southwestern coastline of Caspian Sea
2022
Abbasi, Akbar | Algethami, Merfat | Bawazeer, Omemh | Zakaly, Hesham M.H.
The distribution pattern of natural radionuclides (²²⁶Ra, ²³²Th, and ⁴⁰K) and anthropogenic radionuclide (¹³⁷Cs) in surface sediment samples from the southwestern coastline of the Caspian Sea were determined to estimate the radiation hazard indices using a high-purity germanium HPGe gamma-ray detector. The activity concentrations of the sediment samples ranged from 22.5 ± 1.0 to 47.4 ± 2.2 Bq kg⁻¹ dry weight (dw) for ²²⁶Ra, 6.5 ± 0.1 to 18.7 ± 0.7 Bq kg⁻¹ dw for ²³²Th, 559.9 ± 30.9 to 233.2 ± 19.4 Bq kg⁻¹ dw for ⁴⁰K, and 0.9 MDL (minimum detection limit) to 2.7 ± 0.1 Bq kg⁻¹ dw for ¹³⁷Cs. Based on the measured values, radiological risk indices were estimated. The mean values for absorbed dose rate, ambient dose equivalent rate, and excess lifetime cancer risk, were calculated as 35.7 nGy h⁻¹, 47.9 nSv h⁻¹, and 0.2, respectively.
Afficher plus [+] Moins [-]Assessment of natural radioactivity and 137Cs in some coastal areas of the Saudi Arabian gulf
2016
Al-Ghamdi, H. | Al-Muqrin, A. | El-Sharkawy, A.
The levels of natural radioactivity have been investigated in some Saudi Arabian Gulf coastal areas. Sampling sites were chosen according to the presence of nearby non-nuclear industrial activities such as, the two main water desalination plants in Al Khobar and Al Jubail, and Maaden phosphate complex in Ras Al Khair, to ensure that effluents discharges into the Arabian Gulf didn't enhance radioactivity in seawater and shore sediments. Seawater samples were analyzed for radium isotopes (Ra-226 & Ra-228) and measured by gamma spectrometry using high purity germanium detector, after radiochemical separation of the isotopes by co-precipitation with MnO2. Shore sediment samples were analyzed for 226Ra, 228Ra (232Th), 4°K and 137Cs using gamma sepectrometry. A small variation was observed in the activity concentrations of the investigated radioisotopes, and the activity levels were comparable to those reported in literature. Quality assurance and methods validation were established through the efficiency calibration of the detectors, the estimation of uncertainties, the use of blanks, the analysis of standard reference materials and the intercomparison and proficiency tests. Radiological hazards were assessed, and the annual effective dose had an average value of 0.02mSv. On the basis of the current results, we may conclude that any radiological hazards to the public visiting these shores are not expected.
Afficher plus [+] Moins [-]Radiological maps in beach sands along some coastal regions of Turkey
2016
Kucukomeroglu, B. | Karadeniz, A. | Damla, N. | Yesilkanat, C.M. | Cevik, U.
In the present research, the gamma-emitting radionuclides in beach sands along the coastal regions of the Ordu, Giresun and Trabzon provinces, Turkey have been determined. The natural and anthropogenic radionuclide concentrations of the samples have been measured employing a germanium (HPGe) detector with high resolution and purity. The activity for 238U, 232Th, 40K and 137Cs of the samples were found to vary in the range from below detection limit (BDL) to 65Bq·kg−1, from BDL to 28Bq·kg−1, from 9 to 1936Bq·kg−1 and from BDL to 22Bq·kg−1, respectively. The activity concentrations were compared with those in the literature. The associated radiological hazard indices were estimated, and were compared to the internationally recommended values. The radiological map of beach sand in the surveyed area was imaged. The data presented in the study are crucial since they constitute a baseline for the radiological mapping of the region in the future.
Afficher plus [+] Moins [-]Atmospheric fallout of radionuclides in peat bogs in the Western Segment of the Russian Arctic
2021
Yakovlev, Evgeny | Spirov, Ruslan | Druzhinin, Sergey | Ocheretenko, Alina | Druzhinina, Anna | Mishchenko, Egor | Zhukovskaya, Evgeniya
This article presents the results of studies of the activity of radionuclides in peat-bog profiles of the European subarctic of Russia. Two peat profiles were collected in different areas of the Arkhangelsk region. The peat cores were used to determine ²¹⁰Pb, ¹³⁷Cs, ²⁴¹Am, ²³⁹Pu, ²⁴⁰Pu, ²³⁸U, and ²³⁴U content. To estimate the relationship between radionuclide activity and physicochemical parameters of peat, the content of organic matter, water-soluble salts, carbonates and ash, and the pH of aqueous and salt extracts were studied. Radionuclide activity concentrations in peat samples were measured using inductively coupled plasma mass spectrometry (ICP-MS), low-background semiconductor gamma spectrometry with a high-purity germanium (HPGe) detector, and alpha spectrometry. The ²¹⁰Pb chronology of peat cores was studied using a constant flow model based on the Monte Carlo simulation method. Comparison of ²¹⁰Pb dating data showed that the position of the maximum activity peaks of anthropogenic radionuclides shifted along the peat profile. This is probably due to the relative mobility of different radionuclides in the peat massif. Measurement of the atomic ratio ²⁴⁰Pu/²³⁹Pu showed that the main sources of pollution in the peatlands of the European subarctic of Russia are global fallout from atmospheric tests from the 1950s through 1980 and fallout from the Chernobyl nuclear accident in 1986. This study shows that a complex of radioactive isotopes in peat deposits can provide valuable information on the environmental pollution loads of subarctic territories.
Afficher plus [+] Moins [-]Natural radioactivity and radiological risks of common building materials used in Semnan Province dwellings, Iran
2021
Imani, Morteza | Adelikhah, Mohammademad | Shahrokhi, Amin | Azimpour, Ghazaleh | Yadollahi, Ali | Kocsis, Erika | Toth-Bodrogi, Edit | Kovács, Tibor
Impact assessment of building materials is a focused topic in the field of radioecology. A radiological survey has conducted to monitor radioactivity of most common building materials in Semnan Province, Iran, and assess the radiation risk. Activity concentrations of ²²⁶Ra, ²³²Th, and ⁴⁰K were measured in 29 samples including nine commonly used building materials that were collected from local suppliers and manufacturers, using a high purity germanium gamma-ray detector. The activity concentrations of ²²⁶Ra, ²³²Th, and ⁴⁰K varied from 6.7±1 to 43.6±9, 5.9±1 to 60±11, and 28.5±3 to 1085±113 Bq kg⁻¹ with averages of 26.8±5, 22.7±4, and 322.4±4 Bq kg⁻¹, respectively. By applying multivariate statistical approach (Pearson correlation, cluster, and principal component analyses (PCA)), the radiological health hazard parameters were analyzed to obtain similarities and correlations between the various samples. The Pearson correlation showed that the ²²⁶Ra distribution in the samples is controlled by changing the ²³²Th concentration. The variance of 95.58% obtained from PCA resulted that the main radiological health hazard parameters exist due to the concentration of ²²⁶Ra and ²³²Th. The resulting dendrogram of cluster analysis also shows a well coincidence with the correlation analysis.
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