As the two most commonly used organophosphorus herbicides, glyphosate (Gly) and glufosinate-ammonium (Glu) have unique properties for weed control and algae removal in aquaculture. However, the occurrences and health risks of Gly and Glu in aquaculture ponds are rare known. This study aimed to investigate the occurrences of Gly, AMPA (primary metabolity of Gly) and Glu in surface water, sediment and aquatic products from the grass carp (ctenopharyngodon idella), crayfish (procambarus clarkii) and crab (eriocheir sinensis) ponds around Lake Honghu, the largest freshwater lake in Hubei province, China where aquaculture has become the local pillar industry. Three age groups (children, young adults, middle-aged and elderly) exposure to these compounds through edible aquatic products (muscle) consumption were also assessed by target hazard quotient (THQ) method. The results indicated that Gly, AMPA and Glu were widely occurred in surface water, sediment and organisms in the fish, crayfish and crab ponds. AMPA was more likely to accumulate in the intestine of aquatic products than Gly and Glu. According to the total THQ value (1.04＞1), muscle consumption of grass carp may pose potential risk to children.

Glufosinate-ammonium (GLA) is a spectrum herbicide that is widely used in agriculture. The toxic effects of GLA on plants and mammals have been extensively studied; however, little is known about its effects on reptiles. In this study, male lizards (Eremias argus) were exposed to GLA contaminated soil for 60 days. Physical conditions, organ coefficients, antioxidant enzyme activity, tissue distribution, histopathological damage, steroid hormones levels, and related gene expression of sex steroids were evaluated. In contrast to unexposed control lizards, the body mass index of the GLA group was decreased, which elucidated that GLA adversely affected the physical condition of E. argus. Changes in antioxidant enzyme activities in response to elevated malondialdehyde levels in lizard testis indicated that testes were strongly affected by oxidative damage, and the increased testis index was associated with severe testis lesions. Moreover, alterations of plasma sex hormone levels and related gene expression levels of sex steroids were also observed, and the mechanism underlying the induction of reproductive toxicity was clarified. The activity of glutamine synthetase was severely inhibited in the liver of the GLA exposure group. Based on the results of liver index and histopathology examinations, the hepatotoxicity effect of GLA was confirmed.

Glyphosate (N-phosphonomethyl glycine) and glufosinate (ammonium dl-homoalanin- 4-methyl phosphinate) are nonselective, broad-spectrum, and highly polar herbicides that are wildly used for weed control in aquatic systems and vegetation control in non-crop areas. Aminomethylphosphonic acid (AMPA) is the major degradation product of glyphosate. To address the concerns to its environmental residue and the possible adverse effects, the analytical methods by using microwave-assisted derivatization were developed for determining glyphosate, AMPA, and glufosinate in river water and sediments. The methods applied the 9-fluorenylmethyloxycarbonyl chloride (FMOC-Cl) derivatization for the analytes. The microwave heating is first-time applied to reduce the FMOC-reaction time of glyphosate, AMPA, and glyphosate in the environmental samples to less than 2.5 min. The microwave-assisted methods were successfully validated for river water and sediment. The linear ranges of 7.8–2000.0 ng/L and 0.78–100.0 ng/g were achieved by using 10 mL of water and 2 g of sediments. Glyphosate was found in 30/32% and 25/32% of 32 water and 32 sediments at 27.1–1353.9 ng/L and 2.4–189.6 ng/g levels. AMPA was found in 30/32% and 30/32% of 32 water and 32 sediments at 60.2–1509.0 ng/L and 1.8–233.6 ng/g levels. Glyphosate was found in 10/32% of 32 water at 14.8–503.1 ng/L levels. No glufosinate residue was observed for 32 sediments. The residues of glyphosate and AMPA were wildly detected in the river waters and sediments near the agricultural regions, and glufosinate was less detected. This is the first study that reported herbicide levels in water and sediment from Taiwan rural areas using microwave-assisted rapid derivatization, useful information for environmental management.

Glyphosate and glufosinate ammonium are the active ingredients of commonly used herbicides. Active agricultural lands extend over a large part of the Veneto region (Eastern Po Valley, Italy) and glyphosate and glufosinate ammonium are widely used. Consequently, surface waters can be potentially contaminated. This study investigates the occurrence of glyphosate and glufosinate ammonium as well as aminomethylphosphonic acid (AMPA, the degradation product of glyphosate) in river water of Veneto. Eighty-six samples were collected in 2015 at multiple sampling points across the region. Samples were analyzed for the two target herbicides, AMPA as well as for other variables, including water temperature, pH, dissolved oxygen, conductivity, hardness, BOD, COD, inorganic ions, total nitrogen, total phosphorus, total suspended solids, arsenic, and lead. The average concentrations (all samples) were 0.17, 0.18, and 0.10 μg L⁻¹ for glyphosate, AMPA, and glufosinate ammonium, respectively. The European upper tolerable level for pesticides (annual average 0.1 μg L⁻¹) was often exceeded. Chemometric analysis was therefore applied to (i) investigate the relationships among water pollutants, (ii) detect the potential sources of water contamination, (iii) assess the effective water pollution of rivers by identifying river basins with anomalous pollution levels, and (iv) assess the spatial variability of detected sources. Factor analysis identified four factors interpreted as potential sources and processes (use of herbicides, leaching of fertilizers, urban/industrial discharges, and the biological activity on polluted or stagnant waters). A discriminant analysis revealed that the pollution from anthropogenic discharges is homogeneously present in surface water of Veneto, while biological activity and fertilizers present heterogeneous distributions. This study gives insights into the concentrations of herbicides in rivers flowing through a wide region that has heavy use of these chemicals in agriculture. The study also points out some hot-spots and suggests the future implementation of the current monitoring protocols and network.

Wastewater-based epidemiology is an innovative approach to estimate a population’s intentional and unintentional consumption of chemicals based on biomarker assays found in wastewater. This method can provide real-time objective information on the xenobiotics to which a population is directly or indirectly exposed. This approach has already been used to assess the population exposure to four classes of pesticides: organochlorines (chlordecone), triazines, organophosphates, and pyrethroids. This review aims to obtain the data (excretion rates) and characteristics (pesticide and metabolites stability, including in-sewer one) for other pesticides to broaden the scope of this new method. Excretion rates and stability descriptions for 14 pesticides, namely 2,4-D, aldrin, carbaryl, chlorobenzilate, dieldrin, diquat, ethion, glufosinate, glyphosate, folpet, malathion, parathion, penconazole, and tebuconazole, will be discussed in a practical framework.

Glufosinate is a non-selective chiral herbicide, which has been used extensively around the world. However, limited information on the enantioselectivity of Rac- and L-glufosinate against crops. In this study, the enantioselective effects on the growth, antioxidant, and targeted enzyme activities of maize seedlings of chiral glufosinate were investigated. The results showed the enantioselective growth inhibitions were observed at both 1 and 5 mg/L concentration levels. L-Glufosinate induced more growth rate reduction in shoot height and weight compared to Rac-glufosinate. All of the antioxidant enzyme activities increased obviously in the leaves of maize seedlings treated by 1 mg/L of glufosinate. Superoxide dismutase (SOD) activity, catalase (CAT) activity, peroxidase (POD) activity, glutathione reductase (GR) activity, and malondialdehyde (MDA) content induced by L-glufosinate were 1.36, 1.16, 1.51, 1.65, and 1.65 times higher than those by Rac-glufosinate, respectively Notably, the glutamine synthetase (GS) activity was significantly reduced to 80% and 57% in the control group at 1 mg/L treated with Rac- and L-glufosinate, respectively. Our results indicated that Rac- and L-glufosinate showed the obvious enantioselectivity in the growth of maize seedlings, which has shed light on the potential enantioselective phytotoxicity of glufosinate. Data provided here will be helpful to develop the environmentally friendly herbicides.

Herbicides are increasingly applied in vineyards worldwide. However, not much is known on potential side effects on soil organisms or on the nutrition of grapevines (Vitis vinifera). In an experimental vineyard in Austria, we examined the impacts of three within-row herbicide treatments (active ingredients: flazasulfuron, glufosinate, glyphosate) and mechanical weeding on grapevine root mycorrhization; soil microorganisms; earthworms; and nutrient concentration in grapevine roots, leaves, xylem sap and grape juice. The three herbicides reduced grapevine root mycorrhization on average by 53% compared to mechanical weeding. Soil microorganisms (total colony-forming units, CFU) were significantly affected by herbicides with highest CFUs under glufosinate and lowest under glyphosate. Earthworms (surface casting activity, density, biomass, reproduction) or litter decomposition in soil were unaffected by herbicides. Herbicides altered nutrient composition in grapevine roots, leaves, grape juice and xylem sap that was collected 11 months after herbicide application. Xylem sap under herbicide treatments also contained on average 70% more bacteria than under mechanical weeding; however, due to high variability, this was not statistically significant. We conclude that interdisciplinary approaches should receive more attention when assessing ecological effects of herbicides in vineyard ecosystems.

The presence of glyphosate-resistant oilseed rape populations in Argentina was detected and characterized. The resistant plants were found as weeds in RR soybeans and other fields. The immunological and molecular analysis showed that the accessions presented the GT73 transgenic event. The origin of this event was uncertain, as the cultivation of transgenic oilseed rape cultivars is prohibited in Argentina. This finding might suggest that glyphosate resistance could come from unauthorized transgenic oilseed rape crops cultivated in the country or as seed contaminants in imported oilseed rape cultivars or other seed imports. Experimentation showed that there are alternative herbicides for controlling resistant Brassica napus populations in various situations and crops. AHAS-inhibiting herbicides (imazethapyr, chlorimuron and diclosulam), glufosinate, 2,4-D, fluroxypyr and saflufenacil proved to be very effective in controlling these plants. Herbicides evaluated in this research were employed by farmers in one of the fields invaded with this biotype and monitoring of this field showed no evidence of its presence in the following years.