Micro and nanoplastics are new generation contaminants of global concern. It is important to evaluate the effects on edible products due to the presence of micro- and nano-sized plastics in the treated wastewater. A hydroponic experiment was carried out to explore the effect of polsytrene nanoplastics (PS-NPs; 20 nm) at different concentrations (0, 12.5, 25, and 50 mg L⁻¹) on Glycine max L. (soybean) seedlings for 7-days. In the current study, firstly the uptake of PS-NPs by Glycine max L. (soybean) roots were confirmed by laser confocal scanning microscope. Exposure to PS-NPs, negatively affected growth parameters and increased Fe, Zn and Mn contents in roots and leaves of soybean seedlings. PS-NPs treatments caused oxidative stress in soybean seedlings. The hydrogen peroxide and malondialdehyde contents, showed similar increase pattern in seedlings exposed to PS-NPs. Response to PS-NPs, the level of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase) and proline content were generally enhanced in roots and leaves of soybean. The expression level of stress-related genes examined in the study included CSD5, FSD3, APX1, and POD up-regulated in PS-NPs treated-soybean seedlings in a tissue specific manner. The results of the present study showed the adverse effects of PS-NPs on soybean seedlings, which may have important implications for the risk assessment of NPs on crop production and environmental safety.

In the current investigation, we presented the success of the modified hydrothermal method for synthesizing the iron-oxide nanoparticles (Fe₂O₃-NPs) efficiently. These NPs were further characterized by using different techniques such as X-ray diffraction (XRD), scanning electron microscope (SEM) micrographs, energy-dispersive X-ray spectroscopy (EDAX)/Mapping pattern, Raman Spectroscopy Pattern, ultra violet (UV) and Photoluminescence (PL). All these analyses revealed highly pure nature of Fe₂O₃-NPs with no internal defects, and suggested its application for plant growth improvement. Therefore, we further investigated the separate as well as combined effects of the Fe₂O₃-NPs and citric acid (CA) in the alleviation of arsenic (As) toxicity in the soybean (Glycine max L.), by evaluating the different plant growth and metabolic attributes. Results of our study revealed that As-induced growth inhibition, reduction of photosynthesis, water use efficiency (WUE), and reactive oxygen species (ROS) accumulation whereas application of the Fe₂O₃-NPs and CA significantly reversed all these adverse effects in soybean plants. Moreover, the As-stress induced malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) production were partially reversed by the Fe₂O₃-NPs and CA in the As-stressed plants by 16% and 10% (MDA) and 29% and 12% (H₂O₂). This might have resulted due to the Fe₂O₃-NPs and CA induced activities of the antioxidant defense in plants. Overall, the Fe₂O₃-NPs and CA supplementation separately and in combination positively regulated the As tolerance in soybean; however, the effect of the combined application on the As tolerance was more profound relative to the individual application. These results suggested the synergetic effect of the Fe₂O₃-NPs and CA on the As-tolerance in soybean. However, in-depth mechanism underlying the defense crosstalk between the Fe₂O₃-NPs and CA needs to be further explored.

The present study evaluated the interactive effects of global change and heavy metals on the growth and development of three soybean [Glycine max (L.) Merrill] cultivars and the consequences on yield and food safety. Soybean cultivars (Alim 3.14 from Argentina, and ES Mentor and Sigalia, from Germany) were grown until maturity in heavy metals polluted soils from the Rhine Valley, Germany, at two CO₂ concentrations (400 and 550 ppm) and heat stress (HS) episodes (9 days with 10 °C higher than maximum regular temperature) during the critical growth period in controlled environmental chambers. Different morpho-physiological parameters, heavy metal concentration in aerial organs, seed quality parameters, and toxicological index were recorded. The results showed that no morphological differences were observed related to CO₂. Moreover, Alim 3.14 showed the highest yield under control conditions, but it was more sensitive to climatic conditions than the German cultivars, especially to heat stress which strongly reduces the biomass of the fruits. Heavy metals concentration in soil exceeds the legislation limits for agricultural soils for Cd and Pb, with 1.6 and 487 mg kg⁻¹ respectively. In all cultivars, soybeans accumulated Cd in its aerial organs, and it could be translocated to fruits. Cd concentration in seeds ranged between 0.6 and 2.4 mg kg⁻¹, which exceed legislation limits and with toxicological risk to potential Chinese consumers. Pb levels were lower than Cd in seeds (0.03–0.17 mg kg⁻¹), and the accumulation were concentrated in the vegetative organs, with 93% of the Pb incorporated. Moreover, pods accumulated 11 times more Pb than seeds, which suggests that they act as a barrier to the passage of Pb to their offspring. These results evidence that soybean can easily translocate Cd, but not Pb, to reproductive organs. No regular patterns were observed in relation to climatic influence on heavy metal uptake.

In modern agricultural practice, heavy metal (HM) contamination is one of the main abiotic stress threatening sustainable agriculture, crop productivity, and disturb natural soil microbiota. Different reclamation techniques are used to restore the contaminated site; however, they are either costly or unable to remove contaminant when concentration is very low. In such circumstances, bioremediation is used as a novel technique involving microbes for soil restoration. In the current project, Aspergillus welwitschiae(Bk) efficiently endure metal stress (i.e., Cr-VI and As-V in the form of K₂Cr₂O₇ and Na₃AsO₄) up to 1200 μg/mL and enhanced the production of phytohormones, i.e., 54.83 μg/mL of indole acetic acid (IAA) compared to control 15.56 μg/mL, solubilized inorganic phosphate, and produced stress-related metabolites. The isolate Bk was able to enhance growth of soybean by showing higher root shoot length and fresh/dry weight under stress (p<0.05). Besides, the strain strengthened the antioxidant system of the host increasing enzymatic antioxidants, i.e., catalases (CAT) by 1.58 and 1.11 fold, ascorbic acid oxidase (AAO) by 6.75 and 7.94 fold, peroxidase activity (POD) by 1.12 and 1.37 fold, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) by 1.42 and 1.25 fold at 50 μg/mL of chromate and arsenate. Thus, actively scavenging the reactive oxygen species (ROS) produced results in lower ROS accumulation and high ROS scavenging. On the other hand, the isolates cut down Cr and As uptake by approximately 50% at 50 μg/mL from the medium while bio-transforming it, thereby stabilizing it and assisting the host to resume normal growth, thus avoiding phytotoxicity. It is evident from the current study that A. welwitschiae may potentially be used as a bioremediating agent for reclamation of Cr- and As-contaminated soil.

Row crop agriculture systems are a significant contributor to non-point source nutrient loading into water bodies. One approach to reduce phosphorus (P) losses through surface runoff is applying flue gas desulfurization (FGD) gypsum as a soil amendment. This research was conducted to examine the effects of different rates of FGD gypsum application to corn (Zea mays L.)–soybean (Glycine max) plots on water quality parameters including dissolved reactive phosphate (DRP), total phosphorus (TP), and total suspended solids (TSS). The study was conducted on a high P level (>30 mg P kg⁻¹) soil in a completely randomized design with four treatments each replicated three times. The four treatments were no FGD gypsum (control), FGD gypsum at a rate of 2.2 Mg ha⁻¹, FGD gypsum at 4.5 Mg ha⁻¹, and FGD gypsum at 13.5 Mg ha⁻¹. Gypsum applications were effective in reducing P loads in surface runoff water, with a significant (P < 0.1) reduction in DRP and TP from all the treatments compared to the control during the initial post-gypsum application period (December 2018–May 2019). Results suggest application rates of 4.5 Mg ha⁻¹ and 13.5 Mg ha⁻¹ were most suitable to reduce P loads in surface runoff water from Hosmer silt loam soil with high soil test P (STP) prior to P fertilizer application. However, following P fertilizer application (May 2019–January 2020), gypsum was not effective in reducing P in surface runoff. Overall, FGD gypsum appeared to be an effective phosphorus abatement tool for southern Illinois soils to improve water quality. Though, how long it remains effective appears to be in question given our results in the post P fertilization period.

The scope of this study is to analyze the climatic potential productivity of soybean [Glycine max (L.) Merr.] and explore the impact of climate change on soybean in the frigid region in China by using daily climatic variables from 144 meteorological stations for the period 1971‒2019. The gradually descending model is used to estimate photosynthesis, light-temperature, and climatic potential productivity of soybean. The results show that climate potential productivity of soybean in the frigid region ranges from large to small: Liaoning > Jilin > Heilongjiang > East Four Leagues (four cities in eastern Inner Mongolia), with Heilongjiang and East Four Leagues showing a significant upward trend. Spatially, the climate potential productivity is larger on plains than that on mountains. The Northeast Plain and Sanjiang Plain are areas with high climate potential productivity. Changes in climatic factors have different impacts on the climate potential productivity of soybean. The influence of temperature changes on the climate potential productivity shows a positive effect, and climate warming compensates for the lack of heat in the frigid region. Furthermore, radiation and precipitation are the main climatic factors leading to spatial differences in the climate potential productivity of soybean in the frigid region. Radiation changes have a positive effect on soybean climate potential productivity in plain areas and a negative effect on the mountains. However, precipitation reduction negatively affects most of the frigid region, while it has a positive effect on the two plains of Heilongjiang. Precipitation responses the needs of soybean growth. Our findings recommend that a transition of soybean planting from the mountainous region to plain, that is, from low potential productivity areas to high potential productivity areas, could be an effective strategy for regional optimization for planting structure and rational utilization of irrigation technology.