Nitrate is a major contributor to water contamination, which can affect humans' and animals' health. Due to increased sewage production, growth of agricultural activities, and development of urbanization, recent years have seen an increase of Nitrate in water resources. Drinking water resources in both rural and urban areas of Jiroft City are supplied by water wells, scattered throughout the region. Thus the present research analyses the Nitrate pollution of 31 drinking water wells in summer and winter of 2016, in the urban area of ​​Jiroft City and by means of GIS as well as statistical analysis, presents the results as zoning and survey maps. It also studies and evaluates the effect of rainfall and soil type on the amount of Nitrate. Results from statistical analyses show that the amount of water pollution to Nitrate is independent from the type of land use as well as the soil type. Furthermore, statistical results show that the amount of Nitrate in the wells under test is affected by precipitation, being higher in the winter. Therefore, considering the agricultural density in this area and the untapped use of nitrogen fertilizers, it is necessary to take into account the use of chemical fertilizers for proper management, scientific and practical control, and maintenance of the wells' health safety.