Natural halloysite nanotubes (HNTs) with a hollow lumen are already applied in numerous fields and enter the environment in increasing quantities, which may have effects on animal and human health. However their in vivo toxicity in mammals is still largely unclear. The aim of this study is to assess acute oral toxicity of HNTs in the stomach of mice and recovery. Oral HNTs at low dose (5 mg HNTs/kg BW) for 30 days increased in daily food and water intake and promoted mouse growth with no obvious adverse effect on the stomach. The promotive effect on mouse growth disappeared after cessation of oral administration of the nanotubes. Oral HNTs for 30 days at high dose (50 mg HNTs/kg BW) induced Si and Al accumulation in the stomach, which caused oxidative stress, inflammation and iNOS-mediated damage in the organ. The damage in the stomach led to slight atrophic gastritis and reduced mouse growth. Oral HNTs-induced changes at high dose were not observed after a 30-days recovery period. The findings provided the evidence that oral HNTs-induced acute toxicity in the stomach was reversible. More importantly, this research showed that Al and Si were cleared out of the mice by hepatic excretion and renal excretion, respectively, during the recovery period. The results suggest that HNTs at low concentration in environments have no adverse effect on mice, while there are health risks to mice under severe contamination by HNTs.

Tremendous efforts have been devoted by researchers on air particulate matter pollution for the increasing harm, however, the Air Pollution Index (API) from “good” to “excellent” is something hard to achieve. Here, halloysite nanotubes/polyvinyl alcohol (HNTs/PVA) hybrid membrane with surface micro-nano structure processing using a one-step method for efficient PM₂.₅ capture was prepared. The filtration efficiency is 45.35% and the pressure drop is 41.57 Pa of composite membrane with a 60 wt% halloysite dosage. Specially, it resulted in a relatively safer PM index value of about 16.54, which tends to be more stringent than the restriction by Government of China (PM₂.₅ < 35 μg/m³). The filtration performance was mainly attributed to the controllable microroughness surface as well as the hierarchical structure constructed by one-step method, which has a functional role in obstruction and adsorption for diluted PM₂.₅. The methodology can employ halloysite onto various polymers, like polyvinyl alcohol (PVA), polyvinylpyrrolidone (PVP), polyacrylonitrile (PAN) and also polycaprolactone (PCL) to yield hybrid membranes with the similar modification of surface and structure. Such versatile membrane filters can be purposely designed and scaled up, which endows the existing hybrid membrane with great potentials in both residential and public areas pollution control to achieve a healthier living environment.

For the first time, in this study, two novel glycogen-graphene oxide/halloysite nanotubes (GO-Gly/HNTs) and guar gum-graphene oxide/halloysite nanotubes (GO-GG/HNTs) nanocomposites were synthesized as the adsorbents for removal of Pb(II) from water, and the ionic liquid was used in the synthesis as a green solvent. According to the SEM, TEM, EDS, BET, zeta potential, FTIR, and XRD results, GO-Gly/HNTs and GO-GG/HNTs were synthesized successfully. Response surface methodology (RSM) was applied to optimize the experimental conditions. Nanocomposites followed the Langmuir equilibrium model and were best fitted to the pseudo-second-order model. According to the thermodynamic model, the adsorption process was endothermic. Due to several features, these two novel nanocomposites can be considered the proper candidate for Pb(II) removal from water and wastewater. First, these nanocomposites have good adsorption capacity for Pb(II) removal, which is 219 mg/g for GO-Gly/HNTs and 315 mg/g for GO-GG/HNTs. Moreover, nanocomposites can be recycled with proper adsorption capacity after four repeated cycles. These materials can be used to remove Pb(II) from water in the presence of other contaminants because nanocomposites have selective tendency toward Pb(II) in the presence of other pollutants such as Cd²⁺, Cu²⁺, Cr²⁺, and Co²⁺. In addition, the presence of Ca²⁺, Mg²⁺, Na⁺, and K⁺ improve Pb(II) removal. Finally, possible mechanisms for each nanocomposite were represented.

In this study, halloysite nanotube (HNT)–loaded chitosan-based nanocomposite membranes were synthesized and used for pervaporative desalination of water. Structural and morphological properties of the nanocomposite membranes were investigated. The effects of the HNT content, feed temperature, and feed NaCl concentration on the flux and salt rejection were investigated. As the HNT content was increased, the degree of swelling decreased. At all temperature values, higher than 99% of salt rejections were achieved. The flux value increased from 1.63 to 4.89 kg/m²h, when the HNT content increased from 0 to 20 wt% at 30 °C. While the highest salt rejection value was obtained as 99.90% using the 10 wt% HNT-loaded nanocomposite membrane, the highest flux value was obtained as 5.81 kg/m²h using the 20 wt% HNT-loaded membrane at 50 °C. The pervaporation desalination results showed that HNT simultaneously increased the swelling resistance and the separation capability of the chitosan membrane.

Graphene oxide (GO)-based membranes provide an encouraging opportunity for oil-in-water emulsion separation with high separation efficiency. In this work, novel hierarchically structured membrane consisting of GO and halloysite nanotubes (HNTs) was successfully fabricated by vacuum-assisted filtration method. XRD and TEM measurements showed the successful intercalation of HNTs into the interlayers of GO nanosheets. With the incorporation of the one-dimensional hollow tubular structure halloysite nanotubes, GO-HNTs(GOH) membrane possessed combined advantages of high oil rejection rate and excellent fouling resistance properties. The permeate fluxes increased from 286.6 L/(m²·h) for GO membrane to 716 L/(m²·h) for GOH membrane. The results indicate that the GOH membranes have great potential applications in water purification and wastewater treatment.

Natural halloysite nanotubes (HNTs) with a hollow lumen have been widely applied in many fields, such as water purification, drug carriers, cosmetics, antibacterial, and scaffolds for tissue engineering. However, their in vivo toxicity is still largely unclear. The aim of this study is to evaluate sub-chronic oral toxicity of HNTs in the small intestine of mice. The results demonstrated that oral HNTs at low dose (5 mg/kg) for 30 days promoted mouse growth with no obvious adverse effect on the small intestine. The promotive effect on mouse growth disappeared after cessation of oral administration of HNTs. Oral HNTs at high dose (50 mg/kg) for 30 days induced aluminum (Al) and silicon (Si) accumulation and oxidative stress in the small intestine, which caused significant increases in the levels of cyclooxygenase-2 (COX-2) and nitric oxide synthase (iNOS) and inflammatory response and iNOS-mediated damages in the organ. Oral HNTs-induced changes in the small intestine at high dose were not observed after a 30-day recovery period. These findings provided the first evidence that oral HNTs-induced sub-chronic toxicity in the small intestine was reversible. The results suggest that HNTs at low concentration in environments have no adverse effect on mice, while there are health risks to mice under severe contamination by HNTs.

Nanostructures of titanium dioxide, polyaniline, halloysite, and carbon nanotubes have an excellent effect to improve environmental protection and surface properties of coatings like anticorrosion and self-cleaning attributes. In this research, we studied the individual effect of adding each nanostructure within the polyurethane matrix and also all nanostructure combinations with each other on the mentioned properties. To reach this aim, the polyurethane nanocomposites were prepared; then, anticorrosion and self-cleaning tests were carried out on the samples. FTIR spectroscopy and FESEM images were used to characterize the nanocomposite additives and coating influence. According to the obtained results, the polyurethane coating modified with titanium dioxide/polyaniline/halloysite nanotube/carbon nanotube nanocomposite showed very higher corrosion potential and resistance and lower corrosion current in the comparison with other coatings. Also, the mentioned polyurethane nanocomposite coating showed good hydrophilicity and it decolorized a dye contaminant with high efficiency which makes them appropriate candidate for the protection of the substrate against environmental destructive factors.

This work investigates influence of different aluminosillicate nanoparticles (NPs) which are found in air in selected workplaces on the properties of the phospholipid (DPPC) monolayer at air–saline interface considered as ex vivo model of the lung surfactant (LS). The measurements were done under physiological-like conditions (deformable liquid interface at 37 °C) for NP concentrations matching the calculated lung doses after exposure in the working environment. Measured surface pressure–area (π–A) isotherms and compressibility curves demonstrated NP-induced changes in the structure and mechanical properties of the lipid monolayer. It was shown that hydrophilic nanomaterials (halloysite and bentonite) induced concentration-dependent impairment of DPPC’s ability of attaining high surface pressures on interfacial compression, suggesting a possibility of reduction of physiological function of natural LS. Hydrophobic montmorillonites affected DPPC monolayer in the opposite way; however, they significantly changed the mechanical properties of the air–liquid interface during compression. The results support the hypothesis of possible reduction or even degradation of the natural function of the lung surfactant induced by particle–phospholipid interactions after inhalation of nanoclays. Presented data do not only supplement the earlier results obtained with another LS model (animal-derived surfactant in oscillating bubble experiments) but also offer an explanation of physicochemical mechanisms responsible for detrimental effects which arise after deposition of inhaled nanomaterials on the surface of the respiratory system.