In this contemporary era, wetlands and the entire aquatic diversity are suffering from major pollution problem. Not only the aggregation of higher population in metropolitan causes the production of plenty of solid, liquid and gaseous wastes, but also the high-technological industries contribute to a mammoth of wastes in the ecosystem. During the process of industrial effluent discharge into the water bodies, the toxic substances available in these wastes can affect the aquatic flora and fauna, resultantly disturbing the entire system and constituting human health hazards indirectly or directly. The objective of this study was to estimate the nutritional value of the liver and intestine of fish which is being discarded as waste during fish processing and pollution status by calculated water quality index (WQI) and comparison of both wetlands during diverse seasons. WQI of Harike Wetland was observed to be 56.68 which indicates that water quality in this wetland is “poor”, while at Nangal Wetland WQI was calculated to be 39.54 and comes under “good” water quality and safe for the entire ecosystem. HPI (heavy metal pollution index) for Harike Wetland was observed 144.9 and for Nangal Wetland was 3.12, indicating heavy load of heavy metal pollution at test sample site. MI (metal index) value was also detected higher at test sample site (4.76) as compared to that at control site (0.22). The mean total n-3 and n-6 polyunsaturated fatty acids (PUFAs) declined in the liver of test fish samples (Harike Wetland) as compared to control fish (Nangal Wetland) samples except in winter season. Similarly, mean total saturated fatty acids (SFAs) were also found to decline significantly (p<0.05) in the liver of test fish sample as compared to control fish sample during autumn and winter seasons. Significant (p<0.05) decline of mean total n-3 PUFAs (except winter) and total n-6 PUFAs (except rainy) in the intestine of test fish sample was observed during all the seasons when compared to control fish samples. However, total SFAs were found to increase in the intestine of test fish samples as compared to control fish samples during all the studied seasons. In the present investigation, fluctuations recorded in the water quality parameters and major groups of FAs in the tissues were due to the geographical location and pollution load in the fish samples taken from Harike Wetland.

The unregulated discharge of untreated municipal sewage water to the natural water bodies is a major threat to the aquatic ecosystems. In the present study, the fingerlings of Labeo rohita were exposed to treated sewage water and 1/10ᵗʰ of LC₅₀ and 1/20ᵗʰ of LC₅₀ of untreated sewage water (UT) obtained from sewage water treatment plant, Ludhiana, India. After determining 96-h LC₅₀ value of UT, fingerlings were divided into four groups: control, treated, 1/10ᵗʰ of LC₅₀ UT and 1/20ᵗʰ of LC₅₀ UT and exposed for a period of 2 months. Our study revealed that 1/10ᵗʰ LC₅₀ UT and 1/20ᵗʰ LC₅₀ UT groups had significant reduction in body weight, total body length, standard body length and gills somatic index in comparison with the control and treated groups. Histopathological alterations in cellular structure of gills such as ruptured primary lamellae, broken secondary lamellae, distorted chloride cells and goblet cells, missing lamellae and disintegrated lamellar epithelium were also observed in 1/10ᵗʰ LC₅₀ UT and 1/20ᵗʰ LC₅₀ UT groups. A significantly high frequency of micronucleated (MN) cells was observed in 1/10ᵗʰ LC₅₀ UT and 1/20ᵗʰ LC₅₀ UT groups along with the presence of binucleated cells (BN), elongated nuclei (EN) and nuclear buds in gill cells. Genotoxic nature of UT was further confirmed from significantly high values of genetic damage index (GDI) and percentage (%) DNA damage in gill cells of fingerlings exposed to sub-lethal concentrations of UT. The study concluded that untreated sewage water has potential to induce anatomical and physiological defects in gills cells and the severity of toxicity increases with the increase in duration of exposure.

Aeromonas hydrophila, an opportunistic fish pathogen, which causes several major diseases including skin ulcer and haemorrhagic septicemia, contributes considerably to the lethality in aquaculture. Chemical and antibiotic treatment employed against A. hydrophila for disease management are expensive and consequently prompted the advent of drug resistance among the pathogens. To overcome these draw backs, alternative aquatic disease control methods using conventional plant-based medicines are focussed. Our present study aimed to augment the fish non-specific immune system with the implementation of methanolic crude extracts of Andrographis paniculata to Labeo rohita, for evaluating their efficacy against A. hydrophila. Histology of major organs of A. hydrophila-infected fish such as the gills and liver displayed severe tissue damage. A. paniculata extracts exhibited the strong antibacterial activity against A. hydrophila even at lower concentrations (50 μl). The extracts also altered the haematological profile of treated infected fishes by increasing the levels of haemoglobin and total erythrocyte-leucocyte counts, along with the phagocytic index. The extracts also had a significant impact on modifying the anatomy and swimming pattern of infected fish, post treatment with the extracts. Also, A. paniculata treated infected fishes in all the plant extract administration methods, viz. injection, oral feeding and diffusion, and reduced the cumulative mortality rate to less than 30%. Even lower concentrations of A. paniculata extracts (50 μl) resulted in maximum relative percentage survival of treated fishes. Therefore, our findings suggest that A. paniculata was effective against A. hydrophila infection in aquaculture, thereby maintaining a healthy status of these fishes in aquaculture.

Recently, the oxidative stress and immunotoxicity biomarkers have been extensively used in embryotoxicity using fish embryos as promising models especially after exposure to chemical-like environmental estrogens. Bisphenol-A (BPA) is an estrogenic endocrine disruptor and is ubiquitous in the aquatic environment. Larvae of Labeo rohita were exposed to low concentrations of BPA (10, 100, 1000 μg/l) for 21 days. Innate immune system, antioxidants parameters, and developmental alterations were used as biomarkers. Exposure to BPA caused developmental abnormalities including un-inflated swim bladder, delayed yolk sac absorption, spinal curvature, and edema of pericardium. Lipid peroxidation increased and activity of catalase (p < 0.05), superoxide dismutase (p < 0.05), and glutathione peroxidase (p < 0.01) decreased after exposure to BPA. Level of reduced glutathione also decreased (p < 0.05) in BPA-exposed group. Lower expression of tumor necrosis factor-α (p < 0.05) and interferon-γ (p < 0.001) was observed in BPA-exposed groups while expression of interleukin-10 increased (p < 0.05) in larvae exposed to 10 μg/l BPA. Moreover, exposure of BPA caused a concentration-dependent increase in expression of heat shock protein 70 (p < 0.05). The present study showed that the exposure to BPA in early life stages of Labeo rohita caused oxidative stress and suppress NF-κB signaling pathway leading to immunosuppression. The results presented here demonstrate the cross talk between heat shock protein 70 and cytokines expression.

Concentrations and profiles of 17 perfluoroalkyl substances (PFAS) including 13 perfluorocarboxylic acids (PFA) and 4 perfluoroalkyl sulfonates (PFS) were determined in whole blood, muscle, and liver samples of four freshwater fish species in West Lake and Yen So Lake (Hanoi, Vietnam). Concentrations of total 17 PFAS in fish blood samples ranged from 5.2 to 29 (median 16) ng/mL. Total 17 PFAS levels in liver samples (4.5; 2.7–6.6 ng/g wet weight) were significantly higher than in muscle samples (1.0; 0.51–2.6 ng/g wet weight). More than 90% PFAS burdens in our fish samples were attributed to muscle and blood rather than liver, but contributions of individual compounds varied greatly. The most predominant substances were perfluorooctanesulfonate (PFOS) and PFA with chain lengths from C10 to C14 (i.e., PFDA, PFUnDA, PFDoDA, PFTrDA, and PFTeDA). There is no significant difference in PFAS concentrations between the studied species (i.e., bighead carp, common carp, rohu, and tilapia), but common carp showed specific PFAS profiles as compared to other species (e.g., higher proportions of PFOS and long-chain PFA such as PFTrDA, PFTeDA, and PFHxDA). Daily intake doses of PFOS and perfluorooctanoic acid (PFOA) through fish consumption were markedly lower than the US EPA reference dose of 20 ng/kg/day. Weekly intakes of the sum of PFHxS, PFOS, PFOA, and PFNA in our study were still lower than the EFSA tolerable weekly intake of 4.4 ng/kg/week.

The present study assessed the impact of fingerling stocking of Indian major carps (Gibelion catla, Labeo rohita, and Cirrhinus mrigala) on fish yield in 24 small reservoirs of Central India. The time series data on fish stocking and yield were collected from the State Fisheries Department, Govt. of Chhattisgarh, for the year 2008 to 2016. The fish yield increased significantly (p < 0.05) from 87.11 to 379.13 kg/ha/year due to stocking indicating significant impact on fish production. The relationships between area, stocking density, and fish yield of reservoirs were also assessed. Stocking had a significant positive impact on fish yield (t test, p < 0.05). The important water quality parameters were also studied to have a conspicuous picture of eco-hydrological characteristics of these selected small reservoirs. The study forms a baseline for the small tropical reservoir fisheries of Central India (Chhattisgarh state) and suggests regional strategies for sustainable fisheries enhancement and management, employment generation, and nutritional security of a large number of fish-eating population.

High contents of azo dyes and heavy metals enter surface waters with the wastewater from dying and dye-manufacturing industries and pose serious threat to fish. In the present study, changes in the ultra-morphological features of the scale have been evaluated as indicators of the stress of lethal and sublethal concentrations of an azo dye Acid Black-1 (AB-1, CI 20470), zinc (Zn), and their mixture AB-1 + Zn to Labeo rohita. Fish were exposed for 96 h to lethal concentration (LC) causing 0–70% mortality, i.e., LC₀, LC₂₀, LC₅₀, and LC₇₀ of AB-1 (4, 6, 8, and 10 mg/L respectively) and Zn (25, 50, 55, and 60 mg/L respectively) and LC₀, LC₅₀, and LC₇₀ of AB-1 + Zn (2 + 15, 2 + 20, and 2 + 25 mg/L respectively). Subchronic exposures of 150 days were given to 1/12, 1/6, and 1/3 of 96 h LC₅₀ values of AB-1, Zn, and AB-1 + Zn. After each exposure, the fish were kept for a recovery period of 90 days. Breakage of circuli, erosion and breakage of lepidonts, uprooting of tubercles, and disappearance of intercircular teeth were observed in all the fish, after 96 h exposure to AB-1, Zn, and AB-1 + Zn. However, damage to focus and holes were common on the scales of Zn-exposed fish. The mixture AB-1 + Zn was more toxic than either of the two as loss of circuli, lepidonts, and intercircular teeth, and sloughing of surface were observed in the scales after 96 h exposure to 2 + 25 mg/L. Damage at this concentration was more than the damage at 8/10 mg/L AB-1 and 55/60 mg/L Zn. After 150 days of exposure, damage due to sublethal concentrations was more than the damage due to all the concentrations of 96 h exposure. Irregular and sloughed circuli were common at 2 + 1.79 and 2 + 3.59 mg/L AB-1 + Zn. Cracks all over the surface (2 + 7.18 mg/L), calcium projections (2 + 3.59 and 2 + 7.18 mg/L), and holes (2 + 7.18 mg/L) hint towards synergistic toxicity of the mixture. It seems that the present dye and metal formed complexes with collagen and osteoblastic cells of the scale that caused an increase in damage during the post-exposure period. Scales of 2 + 7.18 mg/L AB-1 + Zn exposed fish were completely devoid of the normal architectural pattern on the 90th day of the recovery period. Changes in the ultra-morphology of scales at LC₀ (0% mortality) and sublethal concentrations show that these are early indicators of the stress of minute quantities of dyes and metals in water. This is a first report on the cumulative toxicity of the two most abundant components of textile industry effluents.

The present study attempted to investigate the changes in temperature conducive to fish habitability during the summer months in a hydrologically modified wetland following damming over a river. Satellite image–driven temperature and depth data calibrated with field data were used to analyse fish habitability and the presence of thermally optimum habitable zones in some fishes, such as labeo rohita, cirrhinus mrigala, tilapia fish, small shrimp, and catfish. The study was conducted both at the water’s surface and at the optimum depth of survival. It is very obvious from the analysis that a larger part of the wetland has become an area that destroyed aquatic habitat during the post-dam period, and existing wetlands have suffered significant shallowing of water depth. This has resulted in a shrinking of the thermally optimum area of fish survival in relation to surface water temperature (from 100.09 to 74.24 km² before the dam to 93.97 to 0 km² after the dam) and an improvement in the optimum habitable condition in the comfortable depth niche of survival. In the post-dam period, it increased from 75.49 to 99.76%. Since the damming effect causes a 30.53 to 100% depletion of the optimum depth niche, improving the thermal environment has no effect on fish habitability. More water must be released from dams for restoration. Image-driven depth and temperature data calibrated with field information has been successfully applied in data sparse conditions, and it is further recommended in future work.

The protective efficacy of dietary naringenin (NG) has been investigated against the toxicity caused by cadmium chloride (CdCl₂) using biomarkers of oxidative stress in the liver, gills and kidney of Labeo rohita. The fish were exposed to environmentally relevant concentrations of CdCl₂ (0.37 and 0.62 mg/L) and simultaneously orally administered with NG (50 mg/kg bw/day) for 60 days. Tissue (gills, liver and kidney) samples were collected on days 15, 30 and 60 of the experiment and analysed for endogenous antioxidants and oxidative stress biomarkers. CdCl₂ exposure for 15 and 30 days induced the development of adaptive mechanism as demonstrated by the enhanced activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase in all three tissues. However, on the 60th day, CdCl₂-induced oxidative damage was stipulated by a decline in the enzyme activities and reduced glutathione (GSH) content significantly (p < 0.05) below control levels along with enhanced levels of lipid peroxidation. Oral administration of NG in toxicant exposed fish significantly restored the altered levels of antioxidants, oxidative enzymes and lipid peroxidation. Besides, integrated biomarker response (IBR) analysis was applied by combining all the biomarkers to indicate the overall stress response index. IBR analysis confirmed the altered levels of biomarkers, the oxidative stress induced by CdCl₂ exposure and the ameliorative potential of NG. The present study suggested that NG might have protective role against Cd-induced oxidative insult which might be ascribed to the ability of NG to chelate metals and scavenge free radicals.

The epidermal mucus of fish performs diverse functions from prevention of mechanical abrasion to limit pathogen invasions. The current experiment was designed to extract skin mucus proteins of three freshwater fish, i.e. common carp (Cyprinus carpio), mrigal (Cirrhinus mrigala) and rohu (Labeo rohita) with organic solvent (methanol) and dissolve in different pH of Tris-HCl buffers to examine the significance of pH in the solubilisation of skin mucus proteins. The protein profiles of different pH solubilised methanol fish skin mucus extracts were determined by SDS-PAGE. The non-specific immune enzymes, alkaline phosphatase, lysozyme and protease of fish skin mucus were compared and this present study demonstrated that these enzymes differed in their activity depending on pH buffers. The higher lysozyme and protease activity were observed at the pH of 8.0 and higher alkaline phosphatase activity in the pH 9.0 of C. mrigala fish skin mucus methanol extract. In addition, the bactericidal activity was evaluated against the pathogens Proteus vulgaris and Pseudomonas aeruginosa. The pH 8.0 of C. mrigala skin mucus extract revealed better bactericidal activity than other fish species mucus pH buffers against both P. vulgaris and P. aeruginosa. In the case of protein profile from SDS-PAGE, based on pH buffers and the solubilisation of proteins, differences in the resolution of bands were observed. The higher alkaline pH of 9.0 showed smeared gel bands in all the three fish skin mucus methanol extract. The present study suggests that methanol extracted C. mrigala fish skin mucus at pH 8.0 showed better innate immune enzymes and bactericidal activity. The additional examinations of C. mrigala skin mucus methanol extract in this pH aids in identifying novel bioactive molecules. This is the study of proteome of three fish species skin mucus in the effect of pH. Further analyses are required to evaluate proteins present in fish skin mucus extracted with methanol and the influence of pH on protein solubility. These findings could be helpful in exploring natural alternatives to antibiotics in aquaculture industry against infectious pathogens.