Two holm oak (Quercus ilex L.) sites in Tuscany, Central Italy, were studied in July 2000. Leaf morphological characteristics (area, dry weight, specific dry weight, water content, epicuticular wax amount, stomatal density) and leaf fluorescence were measured. Ozone flux into the leaves was calculated on a stomatal conductance basis. Among the measured parameters, only stomatal density and wax amount significantly differed between the sites. This response might indicate an adaptation to air pollutants, of which tropospheric ozone might be of the greatest importance

The results showed that ozone specific symptoms can be detected in the needles of mature spruces in the southern Sweden with AOT40 considerably lower than the present critical level of 10 000 nl 1sup-1. The results could be expressed as an index per tree and needle generations, but also as a single value for forest stand allowing statistical tests. The results from this study support the general hypothesis and results from exposure and field studies that low nutrient concentrations or nutrient deficiency modifies the ozone response in plants. The results also show that together with low nutrition status ozone may affect the hardiness status of the needles

Needle retention, needle density, height and radial increments were surveyed on Scots pine (Pinus sylvestris L.) in four forest regions, In the each forest region research was conducted on seven about 45-year-old pines. The Needle Trace Method (NTM) was used to determine needle retention and needle density along the main stem retrospectively for the last four decades. The NTM invented by Kurkela and Jalkanen is based on the examination of the vascular bundles connecting the needles and shoot pith. There are presented the long-term average values of needle retention in the particular forest regions, their differences. Needle retention and needle density reflected air pollution stress on the pines