There is currently great interest in replacing fossil-oil with renewable fuels in energy production. Fast pyrolysis bio-oil (FPBO) made of lignocellulosic biomass is one such alternative to replace fossil oil, such as heavy fuel oil (HFO), in energy boilers. However, it is not known how this fuel change will alter the quantity and quality of emissions affecting human health. In this work, particulate emissions from a real-scale commercially operated FPBO boiler plant are characterized, including extensive physico-chemical and toxicological analyses. These are then compared to emission characteristics of heavy fuel-oil and wood fired boilers. Finally, the effects of the fuel choice on the emissions, their potential health effects and the requirements for flue gas cleaning in small-to medium-sized boiler units are discussed.The total suspended particulate matter and fine particulate matter (PM₁) concentrations in FPBO boiler flue gases before filtration were higher than in HFO boilers and lower or on a level similar to wood-fired grate boilers. FPBO particles consisted mainly of ash species and contained less polycyclic aromatic hydrocarbons (PAH) and heavy metals than had previously been measured from HFO combustion. This feature was clearly reflected in the toxicological properties of FPBO particle emissions, which showed less acute toxicity effects on the cell line than HFO combustion particles. The electrostatic precipitator used in the boiler plant efficiently removed flue gas particles of all sizes. Only minor differences in the toxicological properties of particles upstream and downstream of the electrostatic precipitator were observed, when the same particulate mass from both situations was given to the cells.

The feasibility of anaerobic digestion on the release of biogas and heavy metals from contaminated rice straw pretreated with NaOH solution was studied. The results show that NaOH pretreatment can significantly boost the release of biogas and heavy metals from rice straw using anaerobic digestion. Under the optimal conditions for biomass pretreated 6% (w/w) NaOH with a solid-to-solution ratio of 1:20, total biogas and methane yields of 446.3 mL/g and 263.5 mL/g volatile solids were achieved, which were 22.18% and 41.59% higher than those of the control without NaOH pretreatment, respectively, and the release percentages of Cd, Pb, Cu, and Zn from rice straw reached 86.95–97.69%. The release of heavy metals from rice straw can contribute to both the degradation of lignin by NaOH pretreatment and the utilization/transformation of lignocellulose via anaerobic digestion. The acidification levels and total volatile fatty acid contents significantly influence on the release of heavy metals. Based on the Illumina HiSeq sequencing analysis, the dominant phyla in the biogas residues were proteolytic (Bacteroidetes) and hydrogen-producing (Firmicutes) bacteria, while the growth of Methanospirillum and Methanosaeta in anaerobically digested effluent was promoted. The results revealed that anaerobic digestion combined with NaOH pretreatment is suitable for the disposal of heavy metal–contaminated biomass.

Wheat straw is an abundant agricultural waste that is rich in lignocellulose. However, its waxy surface, highly crystallized structure, and limited surface area make it difficult to be hydrolyzed and used efficiently by microorganisms. Liquid hot water (LHW) pretreatment was studied to explore the feasibility of improving the methane yield of wheat straw in anaerobic digestion (AD). The results showed that the crosslinking structure of wheat straw was broken by LHW pretreatment. Some pores and cracks appeared on the surface of the pretreated wheat straw, increasing the microbial attachment sites. Under different hydrothermal temperatures (150–225 °C) and retention times (5–60 min), the degradation of hemicellulose ranged from 27.69 to 99.07%. The maximum methane yield (201.81 mL CH₄/g volatile solids) was achieved after LHW pretreatment at 175 °C for 30 min, which was a 62.9% increase compared with non-treated straw. LHW at high temperatures such as 225 °C was not suitable for the AD of wheat straw. Methane yield results were fitted with the first-order and modified Gompertz equations to evaluate the hydrolysis rate and inhibitory effects of the pretreated materials in AD.

When food waste (FW) undergoes anaerobic digestion, the hydrolysis rate is rapid, and thus causes system instability. Sophora flavescens residues (SFRs) are rich in complex hydrolysed substances, such as lignocellulosic material. When combined FW and SFRs can effectively improve the stability of digestion systems and increase biogas yields. In this work, batch anaerobic experiments were conducted at different co-substrate ratios to investigate the performance of co-digestion and the synergistic effect of FW and SFRs. The co-digestion of the two substrates exerted synergistic effects on biogas production and the highest synergy was 120.8%. After digestion, the ratio of hydrolysed chemical oxygen demand (COD) to the entire COD (RCODH) of the co-digestion group was 1.08 times that of the single FW group, which indicated the co-digestion promoted the hydrolysis of substrates. Moreover, the hydrolysis rate constant (kₕ) of co-digestion group increased by 4.10 times in comparison with that of the single FW group, which indicated the co-digestion increased the hydrolysis rate. In other words, the synergistic effect mainly occurred in the hydrolysis acidification process.

Microbial-derived biodiesel was tested on a lab scale CI diesel engine for carrying out exhaust emission and performance characteristics. The performance, emission, and combustion characteristics of a single cylinder four stroke fixed compression ratio engine when fueled with microbial bio-diesel and its 10–30% blends with diesel (on a volume basis) were investigated and compared with conventional diesel. The bio-diesel was obtained from microbes which were grown by combining distillery spent wash with lignocellulosic hydrolysate at nutrient deprived conditions. The microbes consumed the wastes and converted the high strength waste water into lipids, which were trans-esterified to form bio-diesel. Testing of microbial bio-diesel blends with ordinary diesel at different loading pressures and the emission characteristics were compared. Results indicate that with increasing of the blends, reduction of HC and CO emissions were observed, whilst brake thermal efficiency maxed out at 20% blending. Further increase of blends showed a tendency of increasing of both emissions in the exhaust stream. The Brake Specific Fuel consumption was observed to decline with blending until 20% and then increased. The nitrogen oxide emissions, however, were found to increase with increasing blend ratios and reached a maximum at 20% blend. The escalation of HC, CO, CO₂, and NOx emissions was also observed at higher blending ratios and higher engine loads. The performance studies were able to show that out of the three blends of biodiesel, 20% biodiesel blend was able to deliver the best of reduced hydrocarbon and carbon monoxide emissions, whilst also delivering the highest Brake thermal efficiency and the lowest Brake Specific Fuel consumption.

The aim of this study was to show the dynamics of changes in the activity of enzymes responsible for C, N, and S metabolism, i.e., cellulase, protease, urease, and arylsulfatase in two lignocellulosic composts as well as changes in the concentration of mineral forms important in plant nutrition (N-NH₄⁺, N-NO₃⁻, S-SO₄²⁻). Most of the enzyme activity was higher during 10 weeks of composting in compost I, containing higher amounts of easily available organic matter than in compost II. Enzymatic activities in compost II remained at a higher level for a longer time, but they increased at a slower rate. Mineral content changes in the compost mass consisted primarily of an increase in N-NO₃⁻ concentration and a decrease in N-NH₄⁺ and S-SO₄²⁻ levels, especially in compost I. The concentration of mineral nitrogen and sulfur forms in compost water extracts was about 10–100 times lower than in the compost mass. At the end of composting, the amount of sulfates in the compost mass was 30 and 150 mg kg⁻¹ dw in compost II and I, respectively. In this context, the composts obtained should be considered valuable for fertilizing soils poor in this component and for cultivating plants with high sulfate S demand.

Herein, activated carbon (AC) and carbon nanotubes (CNTs) were synthesised from potato peel waste (PPW). Different ACs were synthesised via two activation steps: firstly, with phosphoric acid (designated PP) and then using potassium hydroxide (designated PK). The AC produced after the two activation steps showed a surface area as high as 833 m² g⁻¹ with a pore volume of 0.44 cm³ g⁻¹, where the raw material of PPW showed a surface area < 4 m² g⁻¹. This can help aid and facilitate the concept of the circular economy by effectively up-cycling and valorising waste lignocellulosic biomass such as potato peel waste to high surface area AC and subsequently, multi-walled carbon nanotubes (MWCNTs). Consequently, MWCNTs were prepared from the produced AC by mixing it with the nitrogen-based material melamine and iron precursor, iron (III) oxalate hexahydrate. This produced hydrophilic multi-wall carbon nanotubes (MWCNTs) with a water contact angle of θ = 14.97 °. Both AC and CNT materials were used in heavy metal removal (HMR) where the maximum lead absorption was observed for sample PK with a 84% removal capacity after the first hour of testing. This result signifies that the synthesis of these up-cycled materials can have applications in areas such as wastewater treatment or other conventional AC/CNT end uses with a rapid cycle time in a two-fold approach to improve the eco-friendly synthesis of such value-added products and the circular economy from a significant waste stream, i.e., PPW. Graphical abstract .

Phosphate, as an additive to composting, could significantly reduce ammonia emission and nitrogen loss but may also cause adverse effects on the degradation of organic matter. However, there is little information about the influence of pH change, salt content, and phosphate on different organic fraction degradation during composting with the addition of phosphate at a higher level. In this study, the equimolar phosphoric acid (H₃PO₄), sulfuric acid (H₂SO₄), and dipotassium phosphate (K₂HPO₄) were added into pig manure composting with 0.25 mol mass per kilogram of dry matter basis addition amount to evaluate the effect of H⁺, PO₄³⁻, and salinity on carbon component transformation and organic matter degradation. The results showed that both H₃PO₄ and K₂HPO₄ additives could lead to shorter duration in the thermophilic phase, lower degradation of lignocellulose, and lesser carbon loss compared to CK, even though had different pH, i.e., acidic and alkaline conditions, respectively. Besides, the addition of H₃PO₄, H₂SO₄, and K₂HPO₄ could increase the degradation of soluble protein and lipid during composting. Redundancy analysis demonstrated that the variation in different organic carbon fractions was significantly correlated with the changes of pH and the presence of PO₄³⁻, but not with SO₄²⁻ and electrical conductivity, suggesting that pH and phosphate were the more predominant factors than salinity for the inhibition of organic matter degradation. Taken together, as acidic phosphate addition produces a true advantage of controlling nitrogen loss and lower inhibition of organics transformation during composting, the expected effects may result in more efficient composting products.

Sugarcane vinasse is the main waste stream of the Brazilian agroindustry. The typical composition of sugarcane vinasse gives it a high polluting potential that implies the necessity to define sustainable strategies for managing this waste. Knowledge of the inorganic and organic composition of vinasse and its seasonal variation is extremely important to conduct scientific research to define alternative managements for vinasse disposal other than fertigation. This study evaluated the variability of vinasse composition throughout the same harvesting season and among three harvesting seasons of one Brazilian annexed biorefinery (2015–2017). The contents of chemical oxygen demand (COD), biochemical oxygen demand (BOD₅), total solids (TS), suspended solids (SS), salinity (K⁺, Na⁺, Ca²⁺, Mg²⁺, Cl⁻, F⁻), nutrients (N, P, S), trace metals (Al³⁺, As²⁺, Ba²⁺, Cd²⁺, Cr³⁺, Co²⁺, Cu²⁺, Fe²⁺, Pb²⁺, Mn²⁺, Hg²⁺, Mo²⁺, Ni²⁺, Se²⁺, Zn²⁺), and specific soluble organic compounds (sugars, alcohols, and organic acids), as well as pH and conductivity, were monitored in 13 samples. The results indicated that sugarcane vinasse is a suitable feedstock for biological treatments, such as anaerobic digestion processes for energy recovery, as well as substrate for biomass (e.g., microalgae, energy crops, lignocellulosic biomass) growth. The application of a previous treatment makes vinasse a more environmentally friendly natural fertilizer for land fertigation.

At present, diminishing oil resources and increasing environmental concerns have led to a shift toward the production of alternative biofuels. In the last few decades, butanol, as liquid biofuel, has received considerable research attention due to its advantages over ethanol. Several studies have focused on the production of butanol through the fermentation from raw renewable biomass, such as lignocellulosic materials. However, the low concentration and productivity of butanol production and the price of raw materials are limitations for butanol fermentation. Moreover, these limitations are the main causes of industrial decline in butanol production. This study reviews butanol fermentation, including the metabolism and characteristics of acetone-butanol-ethanol (ABE) producing clostridia. Furthermore, types of butanol production from biomass feedstock are detailed in this study. Specifically, this study introduces the recent progress on the efficient butanol production of “designed” and modified biomass. Additionally, the recent advances in the butanol fermentation process, such as multistage continuous fermentation, metabolic flow change of the electron carrier supplement, continuous fermentation with immobilization and recycling of cell, and the recent technical separation of the products from the fermentation broth, are described in this study.