The aim of this study was to assess the soil contamination caused by potentially toxic elements (Al, As, Co, Cr, Cu, Fe, Mn, Mo, Ni, Se, V, and Zn) using various indices and the associated risk of human health for adults and children in selected soils from Germany (Calcic Luvisols, Tidalic Fluvisols, Haplic Gleysols, and Eutric Fluvisols) and Egypt (Haplic Calcisols, Sodic Fluvisols, and Eutric Fluvisols). Soil contamination degree has been assessed using indices such as contamination factor (CF), pollution load index (PLI), geo-accumulation index (Igₑₒ), and enrichment factor. We also assessed the health risk for children and for male and female adults. Chromium, Cu, As, Mo, Ni, Se, and Zn in the German Fluvisols had high CF of >6, while in the Egyptian Fluvisols Se, Mo, As, and Al revealed a high CF. The PLI (1.1–5.2) was higher than unity in most soils (except for Tidalic Fluvisols), while the most important contributor was Se, followed by Mo and As in the Egyptian Fluvisols, and by Cr, Cu, and Zn in the German Fluvisols. The median value of hazard index (HI) for children in the studied soils indicated an elevated health risk (higher than one), especially in the German Fluvisols (HI = 4.0–29.0) and in the Egyptian Fluvisols (HI = 2.2–5.2). For adults, median HIs in all soils were lower than unity for both males and females. The key contributor to HI was As in the whole soil profiles, accounting for about 59% of the total HIs in all three person groupings. Our findings show that in the studied multi-element contaminated soils the risk for children’s health is higher than for adults; while mainly As (and Al, Cr, Cu, and Fe) contributed significantly to soil-derived health risk.

Soils may be contaminated by human or veterinary pharmaceuticals. Their behaviour in soil environment is largely controlled by sorption of different compounds in a soil solution onto soil constituents. Here we studied the sorption affinities of 4 pharmaceuticals (atenolol, trimethoprim, carbamazepine and sulfamethoxazole) applied in solute mixtures to soils taken from different horizons of 3 soil types (Greyic Phaeozem on loess, Haplic Luvisol on loess and Haplic Cambisol on gneiss). In the case of the carbamazepine (neutral form) and sulfamethoxazole (partly negatively charged and neutral), sorption affinity of compounds decreased with soil depth, i.e. decreased with soil organic matter content. On the other hand, in the case of atenolol (positively charged) and trimethoprim (partly positively charged and neutral) compound sorption affinity was not depth dependent. Compound sorption affinities in the four-solute systems were compared with those experimentally assessed in topsoils, and were estimated using the pedotransfer rules proposed in our previous study for single-solute systems. While sorption affinities of trimethoprim and carbamazepine in topsoils decreased slightly, sorption affinity of sulfamethoxazole increased. Decreases in sorption of the two compounds could be attributed to their competition between each other and competition with atenolol. Differences between carbamazepine and atenolol behaviour in the one- and four-solute systems could also be explained by the slightly different soil properties in this and our previous study. A great increase of sulfamethoxazole sorption in the Greyic Phaeozem and Haplic Luvisol was observed, which was attributed to elimination of repulsion between negatively charged molecules and particle surfaces due to cation sorption (atenolol and trimethoprim) on soil particles. Thus, our results proved not only an antagonistic but also a synergic affect of differently charged organic molecules on their sorption to soil constituents.

Plants are a key link in the trophic chain and therefore may determine the global circulation of pollutants, including heavy metals (HMs). In the context of sustaining soil functions associated with food safety, the bioavailability of HMs should be reduced to a minimum needed for adequate plant nutrition. The objective of the study was to analyse the bioavailability of zinc, lead and cadmium in phacelia (Phacelia tanacetifolia Benth.) under conditions of varied soil pH and doses of brown coal-based organo-mineral amendment so-called the Rekulter. The experiment was carried out on Haplic Luvisols in field stone pots that sank into the ground, with the following HM content (in mg kg⁻¹ of soil): 90.0 (Zn), 60.4 (Pb) and 0.80 (Cd). The Rekulter was applied to the soil in the amounts of 180, 360 and 720 g per pot. The bio-accumulation index (BI) was calculated as a ratio of a HM content in a plant to its total content in a soil sample, and it was used to evaluate bioavailability. The application of the Rekulter reduced the bioavailability of the studied heavy metals: the lowest BI values were found in the case of Pb. The uptake of HMs by phacelia was the smallest for the highest applied Rekulter dose at a soil pH of approximately 6.0. The bioavailability of Zn, Pb and Cd was influenced by soil pH and organic matter content, reducing their mobility and possible environmental risks. The Rekulter reduced HM bioavailability: the lowest bio-accumulation index (BI) values were found in the case of Pb. The application of the Rekulter into soil improved the physical, chemical and biological properties of soil, including the reduction of contaminant bioavailability.

Microplastics (MPs) have long been the subject of scientific articles dealing with environmental pollution. The purpose of this study was to determine the occurrence and amount of MPs in soil aggregates depending on land use. The soil in the research area was formed on loess parent material and classified as Haplic Luvisol. From the soil samples were determined particle size distribution curves, and subsequently 40 mixed samples were dry sieved and then wet sieved to determine the percentage of individual soil fractions of water-stable soil aggregates (WSA). The representation and number of MPs were determined for the most common fractions. It was found that MPs occur in both cases of land use, slightly more (62 pt/5 g) in forest soil compared to arable (40 pt/5 g). It is generally known that soil is not homogeneous, but the amount of MPs in top layer for arable soil (8000 pt/kg) and forest (12,400 pt/kg) was estimated. The effect of land use is that forest WSA have a larger mean weight diameter (MWD) than arable land. By being larger, they are also more stable to movement during water erosion. MPs are bound in soil aggregates and they move together with them. This can be deduced from our measurements, because MPs extracted from WSA do not disintegrate in water after 2 hours and even after subsequent wet sieving. The effect of land use on microplastic occurrence and movement should be continually of concern in the future.

Chemical cross-linking and the high molecular weight of superabsorbent copolymers (SAPs) are the two main causes of their resistance to biodegradation. However, SAP particles are colonized by microorganisms. For the purposes of this study, the dry technical copolymer of acrylamide and potassium acrylate containing 5.28 % of unpolymerized monomers was wrapped in a geotextile and incubated in unsterile Haplic Luvisol soil as a water absorbing geocomposite. The highest number of soil bacteria that colonized the hydrated SAP and utilized it as the sole carbon and energy source was found after the first month of incubation in soil. It was equal to 7.21–7.49 log₁₀ cfu g⁻¹ of water absorbed by the SAP and decreased by 1.35–1.61 log₁₀ units within the next 8 months. During this time, the initial SAP water holding capacity of 1665.8 g has decreased by 24.40 %. Moreover, the 5 g of SAP dry mass has declined by 31.70 %. Two bacteria, Rhizobium radiobacter 28SG and Bacillus aryabhattai 31SG isolated from the watered SAP were found to be able to biodegrade this SAP in pure cultures. They destroyed 25.07 and 41.85 mg of 300 mg of the technical SAP during the 60-day growth in mineral Burk’s salt medium, and biodegradation activity was equal to 2.95 and 6.72 μg of SAP μg⁻¹ of protein, respectively. B. aryabhattai 31SG and R. radiobacter 28SG were also able to degrade 9.99 and 29.70 mg of 82 mg of the ultra-pure SAP in synthetic root exudate medium during the 30-day growth, respectively.

The difference in effect on acidification and species of aluminum speciation between HNO₃ and H₂SO₄for two contrasting types of soils in surface charge was investigated. The results show that the effect of H₂SO₄ on acidification of variable charge soils (Ferric Acrisol and Haplic Acrisol) of subtropical regions wasweaker than that of HNO₃, due to the specific adsorption of SO₄ ²⁻ and the accompanied release of OH⁻. For two constant charge soils, Haplic Luvisol andEutric Cambisol, the difference in effect between the two acids is small. The concentrations of total inorganic monomeric aluminum, Al³⁺, Al-F complexes and Al-SO₄complexes in the extract from variable charge soils are alsolower in H₂SO₄ systems than those in HNO₃ systems, whereas the reverse is true for constant charge soils, except the concentration of Al³⁺. For variable charge soils, Al-F complexes are the major species of inorganic monomeric aluminum at high pH butAl³⁺ might contribute to a largepart at low pH, while for constant charge soils Al-F complexes contribute almost exclusively to the inorganic monomeric aluminum. The presence of a large amount of SO₄ ²⁻ in the extract from the constant charge soilsleads to a higher proportion of Al-SO₄ complexes in totalinorganic monomeric aluminum than that from variable charge soil, although the numerical value of proportion is small.

The aim of this work was to assess the suitability of Miscanthus × giganteus and Spartina pectinata link to Cu, Ni, and Zn phytoremediation. A 2-year microplot experiment with the tested grasses growing on metal-contaminated soil was carried out. Microplots with cement borders, measuring 1 × 1 × 1m, were filled with Haplic Luvisols soil. Simulated soil contamination with Cu, Ni, and Zn was introduced in the following doses in mg kg⁻¹: 0—no metals, Cu₁—100, Cu₂—200, Cu₃—400, Ni₁—60, Ni₂—100, Ni₃—240, Zn₁—300, Zn₂—600, and Zn₃—1200. The phytoremediation potential of grasses was evaluated using a tolerance index (TI), bioaccumulation factor (BF), bioconcentration factor (BCF), and translocation factor (TF). S. pectinata showed a higher tolerance to soil contamination with Cu, Ni, and Zn compared to M. × giganteus. S. pectinata was found to have a high suitability for phytostabilization of Zn and lower suitability of Cu and Ni. M. × giganteus had a lower phytostabilization potential than S. pectinata. The suitability of both grasses for Zn phytoextraction depended on the age of the plants. Both grasses were not suitable for Cu and Ni phytoextraction. The research showed that one-season studies were not valuable for fully assessing the phytoremediation potential of perennial plants.

Sulfonylurea herbicides are widely used for weed control in agriculture, and they are suspected to alter microbial communities and activities in the soil. This study investigates the impact of two sulfonylurea herbicides chlorsulfuron and sulfosulfuron on microbial community and activity in two different soils taken from two sites in west part of the Slovak Republic. The soil from the Malanta site was silt-loam luvisol with pH₍H₂O₎ 5.78 while the soil from the Stefanov site was sandy-loam regosol with pH₍H₂O₎ 8.25. These soils were not treated by sulfonylurea herbicides at least for 2 years prior to the study. In laboratory assay, the herbicides were applied to soil in their maximal recommended doses 26 and 25 g per hectare of chlorsulfuron and sulfosulfuron, respectively. Their effect was evaluated on the 3rd, 7th, 14th, 28th, 56th, and 112th day after application to soil. Illumina high-throughput amplicon sequencing of the 16S rRNA gene and ITS region was used to monitor changes on prokaryotic and fungal community composition. Enzymatic activity was evaluated using 11 substrates. Physiological profile of microbial community was analyzed using Biolog© ecoplates. Significant changes in enzymatic activity caused by the application of herbicides were found during the first 28 days. The application of herbicides altered the activity of cellobiohydrolase, arylsulphatase, dehydrogenase, phosphatase, and FDA hydrolase. Chlorsulfuron caused a more varying response of enzymatic activity than sulfosulfuron, and observed changes were not the same for both soils. In Malanta soil, chlorsulfuron decreased dehydrogenase activity while it was increased in the Stefanov soil. Phosphatase activity was decreased in both soils on 7th and 14th day. There were only minor changes in prokaryotic or fungal community or physiological profiles regarding pesticide application. Differences between soils and incubation time explained most of the variability in these parameters. Diversity indices, physiological parameters, and enzymatic activity decreased over time. The results have shown that chlorsulfuron and sulfosulfuron can affect the function and activity of the soil microbial community without significant change in its composition.

On the basis of a previous study performed in our laboratory, the use of organic and inorganic amendments can significantly modify the Hg mobility in soil. We have compared the effectiveness of organic and inorganic amendments such as digestate and fly ash, respectively, reducing the Hg mobility in Chernozem and Luvisol soils differing in their physicochemical properties. Hence, the aim of this work was to compare the impact of digestate and fly ash application on the chemical and biochemical parameters in these two mercury-contaminated soils in a model batch experiment. Chernozem and Luvisol soils were artificially contaminated with Hg and then incubated under controlled conditions for 21 days. Digestate and fly ash were applied to both soils in a dose of 10 and 1.5 %, respectively, and soil samples were collected after 1, 7, 14, and 21 days of incubation. The presence of Hg in both soils negatively affected to processes such as nitrification, provoked a decline in the soil microbial biomass C (soil microbial biomass C (MBC)), and the microbial activities (arylsulfatase, and β-glucosaminidase) in both soils. Meanwhile, the digestate addition to Chernozem and Luvisol soils contaminated with Hg improved the soil chemical properties (pH, dissolved organic carbon (DOC), N (Nₜₒₜ), inorganic–N forms (N–NH₄ ⁺ and N–NO₃ ⁻)), as consequence of high content in C and N contained in digestate. Likewise, the soil MBC and soil microbial activities (dehydrogenase, arylsulfatase, and β-glucosaminidase) were greatly enhanced by the digestate application in both soils. In contrast, fly ash application did not have a remarkable positive effect when compared to digestate in Chernozem and Luvisol soil contaminated with mercury. These results may indicate that the use of organic amendments such as digestate considerably improved the soil health in Chernozem and Luvisol compared with fly ash, alleviating the detrimental impact of Hg. Probably, the chemical properties present in digestate may determine its use as a suitable amendment for the assisted-natural attenuation of mercury-polluted soils.