International audience | Aquatic wildlife is exposed through trophic transfer of hazardous substances to several threats inducing physiological impairments. We aimed at assessing the impact of contamination in one of the hot spots of pollution along Mediterranean coasts, the gulf of Gabes in Tunisia, on Common tern Sterna hirundo, a piscivorous top predator bird. Firstly, we compared the reproductive effort of breeding adults through clutch size distribution in three sites with different levels of pollution. Then, a battery of genotoxicity and oxidative stress biomarkers was carried out to assess physiological impairments in chicks. While defense mechanisms showed a depletion, lipid peroxidation and genotoxicity increased significantly according to pollution level. The multi-biomarker approach used here, discriminated chicks according to contamination degree of their nesting sites. Increases in genotoxicity and oxidative stress were correlated to a decrease in chick body mass known to lead to long-term impacts on juvenile survival and recruitment in birds.

International audience | Aquatic wildlife is exposed through trophic transfer of hazardous substances to several threats inducing physiological impairments. We aimed at assessing the impact of contamination in one of the hot spots of pollution along Mediterranean coasts, the gulf of Gabes in Tunisia, on Common tern Sterna hirundo, a piscivorous top predator bird. Firstly, we compared the reproductive effort of breeding adults through clutch size distribution in three sites with different levels of pollution. Then, a battery of genotoxicity and oxidative stress biomarkers was carried out to assess physiological impairments in chicks. While defense mechanisms showed a depletion, lipid peroxidation and genotoxicity increased significantly according to pollution level. The multi-biomarker approach used here, discriminated chicks according to contamination degree of their nesting sites. Increases in genotoxicity and oxidative stress were correlated to a decrease in chick body mass known to lead to long-term impacts on juvenile survival and recruitment in birds.

Plastic pollution is prevalent worldwide and affects marine wildlife from urbanized beaches to pristine oceanic islands. However, the ecological basis and mechanisms that result in marine animal ingestion of plastic debris are still relatively unknown, despite recent advances. We investigated the relationship between scavenging behavior and plastic ingestion using green turtles, Chelonia mydas, as a model. Diet analysis of C. mydas showed that sea turtles engaging in scavenging behavior ingested significantly more plastic debris than individuals that did not engage in this foraging strategy. We argue that opportunistic scavenging behavior, an adaptive behavior in most marine ecosystems, may now pose a threat to a variety of marine animals due to the current widespread plastic pollution found in oceans.

Microplastic particles are a global concern due to their widespread and growing threat to marine and coastal environments. To improve knowledge of microplastic pollution in China, we investigated 25 sediment samples collected with a box corer in the Southern Yellow Sea and East China Sea off the coast of China. The microplastics were extracted from sediments via density separation, after which they were observed under a microscope and characterized according to shape, color, and size, while polymer type identification was performed using micro-Fourier transform infrared spectroscopy. The abundance of microplastics in the offshore region of the Southern Yellow Sea and East China Sea was mapped. The mean concentration of microplastics at the 25 sites was 13.4 ± 0.6 particles 100 g⁻¹ dry weight (range: 6.0–24.0 particles 100 g⁻¹ dry weight). Based on the categorization according to shape, color, and size, fiber (77%) was the most abundant shape, while blue (35%) and transparent (29%) were the most prevalent colors. In addition, the dominant size of microplastics was smaller than 1000 μm which accounted for 89%. Finally, polyethylene, polyethylene terephthalate, acrylic, polyester, cellulose, and cellophane were the most abundant types of microplastics identified. Our result highlighted the presence of microplastics in offshore sediments from the Yellow Sea and East China Sea, and provided useful information for evaluating the environmental risks posed by microplastics in China.

The aim of the present study was to optimize the protocol for sampling marine macroalgae to be used to biomonitor heavy metal contamination in marine ecosystems. For this purpose, we collected 50 subsamples of the brown seaweed Fucus vesiculosus at random in each of three sampling sites (SS) and determined the concentrations of Al, As, Cd, Co, Cr, Cu, Fe, Hg, N, Ni, Pb, Zn and δ15N. We used semivariograms to explore the possible existence of spatial structure in the concentrations of the elements. Spatial structure was observed in 88% of the semivariograms studied, with element concentrations varying longitudinally and transversally along the SS. Using randomization techniques, we estimated that in each SS, a minimum of 30 evenly distributed subsamples should be collected within three bands parallel to the coastline (and also at different heights on the rocks if necessary), and analyzed in a single composite sample representative of the intra-SS variability.

Microplastics are emerging contaminants in the marine environment. They enter the ocean in a variety of sizes and shapes, with plastic microfiber being the prevalent form in seawater and in the guts of biota. Most of the laboratory experiments on microplastics has been performed with spheres, so knowledge on the interactions of microfibers and marine organisms is limited. In this study we examined the ingestion of microfibers by the sea anemone Aiptasia pallida using three different types of polymers: nylon, polyester and polypropylene. The polymers were offered to both symbiotic (with algal symbionts) and bleached (without algal symbionts) anemones. The polymers were introduced either alone or mixed with brine shrimp homogenate. We observed a higher percentage of nylon ingestion compared to the other polymers when plastic was offered in the absence of shrimp. In contrast, we observed over 80% of the anemones taking up all types of polymers when the plastics were offered in the presence of shrimp. Retention time differed significantly between symbiotic and bleached anemones with faster egestion in symbiotic anemones. Our results suggest that ingestion of microfibers by sea anemones is dependent both on the type of polymers and on the presence of chemical cues of prey in seawater. The decreased ability of bleached anemones to reject plastic microfiber indicates that the susceptibility of anthozoans to plastic pollution is exacerbated by previous exposure to other stressors. This is particularly concerning given that coral reef ecosystems are facing increases in the frequency and intensity of bleaching events due to ocean warming.

Artificial light at night (ALAN) is a recently acknowledged form of anthropogenic pollution of growing concern to the biology and ecology of exposed organisms. Though ALAN can have detrimental effects on physiology and behaviour, we have little understanding of how marine organisms in coastal areas may be impacted. Here, we investigated the effects of ALAN exposure on coral reef fish larvae during the critical recruitment stage, encompassing settlement, metamorphosis, and post-settlement survival. We found that larvae avoided illuminated settlement habitats, however those living under ALAN conditions for 10 days post-settlement experienced changes in swimming behaviour and higher susceptibility to nocturnal predation. Although ALAN-exposed fish grew faster and heavier than control fish, they also experienced significantly higher mortality rates by the end of the experimental period. This is the first study on the ecological impacts of ALAN during the early life history of marine fish.

Various biogeochemical processes complicate carbon dioxide (CO₂) behaviour in coastal oceans. Through eight summer surveys, detailed variations in CO₂ mechanisms in the urbanized Jiaozhou Bay, China, were analysed. During the rainless period, respiration and dissolved inorganic carbon input from treated wastewater made the northeastern region a strong CO₂ source, while the western region with cleaner seawater was a weak source because calcium carbonate (CaCO₃) precipitation exceeded primary production. Rainfall events with different intensities and locations caused significantly different effects. When rainfall occurred over the sea, enhanced primary production caused a CO₂ sink; when rainfall induced little terrestrial pollutant input, CaCO₃ precipitation exceeded net primary production, leading to a CO₂ source. When heavy rain caused bulk runoff, the northeastern region was a strong CO₂ source because rivers flowing through downtown regions inputted considerable organic matter, while in the western region, runoff through suburbs and wetlands led to a strong sink.

We assess how different micropollutants and microplastics, connected to wastewater are introduced into the Baltic Sea. The relevance of untreated wastewater, treated wastewater, treated and untreated rain runoff, as well as combined sewer overflow (CSO), is assessed in respect to mass balance, as well as relative inflows of micropollutants and -plastics into the Baltic Sea. To achieve this, modelling based on data on exemplary sewer systems and measured micropollutant concentrations in the single sources were used. Most compounds reach the receiving Baltic Sea via treated wastewater. A few exceptions are compounds that are removed to a very high extent in wastewater treatment plants. For these compounds, the emissions with stormwater (e.g., terbutryn) or untreated wastewater (e.g., triclosan) are dominating. Additionally, compounds that are discharged with the water that is running off urban surfaces are introduced into marine areas via rain runoff. These data are used to forecast a total mass load and concentrations that can be expected in the Baltic Sea. Massloads are expected to be between 0.1 and 5.9 t/a for triclosan and TCPP (tris (2-chloropropyl) phosphate) and 0.2 t/a for microplastic particles. The expected concentrations in open Baltic Sea waters range from 0.01 to 26 ng/L.

Thanks to several European directives and conventions there is a general increase of awareness regarding the key ecological role of coralligenous habitats in the Mediterranean Sea, addressing several research projects to standardize protocols for the description of its integrity. Here we surveyed 13 stations along the Italian coasts of the Western Mediterranean Sea, using video-transects technique, comparing the biological structure of coralligenous assemblages and testing the importance of their three-dimensional complexity as a proxy to define their health conditions. We considered the diversity of taxa, fishing impacts and the entity of damage on gorgonian's choenenchyme due to thermal stress, to evidence a gradient in the coralligenous health conditions. Here we developed a method to evaluate coralligenous complexity, selecting categories of taxa particularly sensitive to multiple stressors, named Structural Descriptors to describe the three-dimensional structure of the bioconcretions and to assess a unique Index of 3D - Structural Complexity.