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Biodegradation of carbamate pesticides by natural river biofilms in different seasons and their effects on biofilm community structure
2013
Tien, Chien-Jung | Lin, Mon-Chu | Chiu, Wan-Hsin | Chen, Colin S.
This study investigated the ability of natural river biofilms from different seasons to degrade the carbamate pesticides methomyl, carbaryl and carbofuran in single and multiple pesticide systems, and the effects of these pesticides on algal and bacterial communities within biofilms. Spring biofilms had the lowest biomass of algae and bacteria but showed the highest methomyl degradation (>99%) and dissipation rates, suggesting that they might contain microorganisms with high methomyl degradation abilities. Degradation of carbofuran (54.1–59.5%) by biofilms in four seasons was similar, but low degradation of carbaryl (0–27.5%) was observed. The coexistence of other pesticides was found to cause certain effects on pesticide degradation and primarily resulted in lower diversity of diatoms and bacteria than when using a single pesticide. The tolerant diatoms and bacteria potentially having the ability to degrade test pesticides were identified. River biofilms could be suitable biomaterials or used to isolate degraders for bioremediating pesticide-contaminated water.
Afficher plus [+] Moins [-]Behavioral and metabolic effects of sublethal doses of two insecticides, chlorpyrifos and methomyl, in the Egyptian cotton leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae)
2016
Dewer, Youssef | Pottier, Marie-Anne | Lalouette, Lisa | Maria, Annick | Dacher, Matthieu | Belzunces, Luc P. | Kairo, Guillaume | Renault, D | Maïbèche, Martine | Siaussat, David | Central Agricultural Pesticides Laboratory (CAPL) | Agricultural Research Center (ARC) | Institut d'écologie et des sciences de l'environnement de Paris (iEES) ; Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS) | Abeilles et environnement (AE) ; Institut National de la Recherche Agronomique (INRA) | Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO) ; Université de Rennes (UR)-Institut Ecologie et Environnement - CNRS Ecologie et Environnement (INEE-CNRS) ; Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR) ; Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS) | DIM ASTREA (Région Ile de France) | Imhotep exchange program grant (Campus France) | EMERGENCE UPMC - Paris 6 | ANR-12-ADAP-0012, PHEROTOX | ANR-12-ADAP-0012,PHEROTOX,Perception de la phéromone dans un environnement contaminé en insecticides : info-perturbation ou adaptation?(2012)
International audience | Insecticides have long been used as the main method in limiting agricultural pests, but their widespread use has resulted in environmental pollution, development of resistances, and biodiversity reduction. The effects of insecticides at low residual doses on both the targeted crop pest species and beneficial insects have become a major concern. In particular, these low doses can induce unexpected positive (hormetic) effects on pest insects, such as surges in population growth exceeding what would have been observed without pesticide application. Methomyl and chlorpyrifos are two insecticides commonly used to control the population levels of the cotton leafworm Spodoptera littoralis, a major pest moth. The aim of the present study was to examine the effects of sublethal doses of these two pesticides, known to present a residual activity and persistence in the environment, on the moth physiology. Using a metabolomic approach, we showed that sublethal doses of methomyl and chlorpyrifos have a systemic effect on the treated insects. We also demonstrated a behavioral disruption of S. littoralis larvae exposed to sublethal doses of methomyl, whereas no effects were observed for the same doses of chlorpyrifos. Interestingly, we highlighted that sublethal doses of both pesticides did not induce a change in acetylcholinesterase activity in head of exposed larvae
Afficher plus [+] Moins [-]Synthetic organic chemicals (flame retardants and pesticides) with neurotoxic potential induced behavioral impairment on zebrafish (Danio rerio): a non-invasive approach for neurotoxicology
2021
Ren, Zongming | Poopal, Rama-Krishnan | Ramesh, Mathan
Behavior responses of organisms can be used as a non-invasive method for neurotoxicology studies since it directly links the nervous system’s functioning and biochemical activities. Among different behavioral activities, aquatic organisms’ swimming behavior (fitness) is the essential factor for health assessment; thus, it is practiced routinely in neurotoxicological studies. Zebrafish (Danio rerio) are excellent models for neurotoxicology studies. Based on the above information, we hypothesized that zebrafish’s swimming behavior is a potential biomarker for neurotoxic effect assessment. We exposed zebrafish (length, 3–4 cm; weight, 0.2–0.3 g) to different synthetic organic chemicals (organophosphorus flame retardants (tri-cresyl phosphate and cresyl diphenyl phosphate) and neurotoxic pesticides (cypermethrin and methomyl) for 15 days. For each test chemical, we chose two different concentrations (Treatment-I 5 μL/L and Treatment-II 25 μL/L) to study their eco-toxicity. The swimming strength of zebrafish was quantified using an online monitoring system. The swimming strength of zebrafish decreased under different treatments (Treatment-I (5 μL/L) and -II (25 μL/L)) of target chemicals. The circadian rhythm of zebrafish was predominantly not affected in this study. Higher neurotoxic effect (behavioral impairment) was observed in Treatment-II when compare to Treatment-I of organophosphorus flame retardants and pesticides groups. Responses of zebrafish under organophosphorus flame retardant (tri-cresyl phosphate and cresyl diphenyl phosphate) treatments were identical with pesticide (cypermethrin and methomyl) treatments. Based on the results, we conclude that swimming behavior could be an ideal non-invasive biomarker to assess waterborne contaminants’ neurotoxic effect.
Afficher plus [+] Moins [-]Identification of Pesticide Residues and Prediction of Their Fate in Agricultural Soil
2020
EL-Saeid, Mohamed H. | Alghamdi, Abdulaziz G.
Fourteen pesticides were screened and determined through quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction process combined with GC-MS/MS in arid agriculture soil. The aims of the current investigation were to account the occurrence of organochlorine (OCP) and organophosphates (OPP) pesticide residues as well as other groups of pyrethroids (PYRs), carbamates, and biopesticides using a combined of QuEChERS and GC-MS/MS techniques in agriculture soils at Al-Kharj region, Saudi Arabia, and to investigate correlation between pesticide losses in soils and some physicochemical characteristics of pesticides including an octanol-water coefficient partition (Kₒw) and the vapor pressure (Vp). Prediction of pesticide fate by considering both pesticide and soil physio-chemical properties will facilitate the management of pesticide application and minimize the hazards of environmental pollution. The fate of pesticide residue in soils is generally controlled by soil/air exchange, water interaction, and biodegradation. The results indicated that 14 pesticide residues were measured in collected samples of various soils, spinosad, chlorpyrifos methyl, dimethoate, chlorpyrifos, lindane (γ-HCH), permethrin, and methomyl which were the most abundant sources of contamination in the studied region. p,p-DDT, o,p-DDT, bifenthion, β-cyfluthrin, and methidathion were less commonly detected. Single parameter least squares regression equations (sp-LSRE) for Vp and Kₒw against the loss of each pesticide residue showed a significant change in concentration levels (p < 0.05) between the two seasons. The results showed that vapor pressure and octanol-water partition coefficient data are not enough to model pesticide residue losses in arid low organic carbon soil. More soil-related data is needed to describe the dissipation mechanisms of these pesticide residues in the region.
Afficher plus [+] Moins [-]Effective photodegradation of methomyl pesticide in concentrated solutions by novel enhancement of the photocatalytic activity of TiO2 using CdSO 4 nanoparticles
2014
Barakat, N. A. M. | Nassar, M. M. | Farrag, T. E. | Mahmoud, M. S.
Annihilation of electrons–holes recombination process is the main remedy to enhance the photocatalytic activity of the semiconductors photocatalysts. Doping of this class of photocatalysts by foreign nanoparticles is usually utilized to create high Schottky barrier that facilitates electron capture. In the literature, because nonpolar nanoparticles (usually pristine metals, e.g., Ag, Pt, Au, etc.) were utilized in the doping process, the corresponding improvement was relatively low. In this study, CdSO₄-doped TiO₂nanoparticles are introduced as a powerful and reusable photocatalyst for the photocatalytic degradation of methomyl pesticide in concentrated aqueous solutions. The utilized CdSO₄nanoparticles form polar grains in the TiO₂matrix due to the electrons leaving characteristic of the sulfate anion. The introduced nanoparticles could successfully eliminate the harmful pesticide under the sunlight radiation within a very short time (less than 1 h), with a removal capacity reaching 1,000 mg pesticide per gram of the introduced photocatalyst. Moreover, increase in the initial concentration of the methomyl did not affect the photocatalytic performance; typically 300, 500, 1,000, and 2,000 mg/l solutions were completely treated within 30, 30, 40, and 60 min, respectively, using 100 mg catalyst. Interestingly, the photocatalytic efficiency was not affected upon multiple use of the photocatalyst. Moreover, negative activation energy was obtained which reveals super activity of the introduced photocatalyst. The distinct photocatalytic activity indicates the complete annihilation of the electrons–holes recombination process and abundant existence of electrons on the catalyst surfaces due to strong electrons capturing the operation of the utilized polar CdSO₄nanoparticles. The introduced photocatalyst has been prepared using the sol–gel technique. Overall, the simplicity of the synthesizing procedure and the obtained featured photocatalytic activity strongly recommend the introduced nanoparticles to treat the methomyl-containing polluted water.
Afficher plus [+] Moins [-]Spatio-temporal distribution and ecological risk assessment of pesticides in the water resources of Abou Ali River, Northern Lebanon
2020
Jabali, Yasmine | Millet, Maurice | El-Hoz, Mervat
The objective of this study is to assess the occurrence, spatial, and temporal distribution of forty-eight multiclass pesticides in surface and groundwater samples of the Abou Ali River, located in the North of Lebanon. A 3-year monitoring program (six batches from August 2015 to March 2017) was implemented, and thirty sampling points were selected along the river for analysis. The analysis was executed using a previously developed and optimized solid-phase micro-extraction (SPME) gas-chromatography-mass spectrometry (GC-MS) method. Statistical analysis, using Kolmogorov–Smirnov, Kruskal–Wallis, and Dunnet T3 multiple comparison tests, was applied to compare mean concentrations of pesticides between the different sampling sites and the batches taken. The pesticides that had the highest frequency of detection in the surface and groundwater samples were alachlor, α-endosulfan, and methomyl. For surface water samples, high mean concentrations were perceived for two stations in the upper stream (S5 and S7), two stations (S11 and S14) in the middle stream, and one station (S16) in the lower stream of the river. The highest mean concentrations were observed in October 2015 and August 2016, the time of the year which correlates with the period of pesticide application. Considering groundwater samples, high mean concentrations of pesticides were detected in sites G4, G9, G10, and G12 and during October 2015 and March 2016, following the rainy season. Ecotoxicological risk assessment using the risk quotient (RQ) methodology revealed high risk for five pesticides under average conditions and fourteen under extreme conditions. This study presents, for the first time, a statistical analysis showing the quantification of pesticides in the water resources of the Abou Ali River. In conclusion, it reveals the need to apply a complete pesticide monitoring program, not only for the Abou Ali River but for all the water resources in Lebanon.
Afficher plus [+] Moins [-]Behavioral and metabolic effects of sublethal doses of two insecticides, chlorpyrifos and methomyl, in the Egyptian cotton leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae)
2016
Dewer, Youssef | Pottier, Marie-Anne | Lalouette, Lisa | Maria, Annick | Dacher, Matthieu | Belzunces, L. P. (Luc P.) | Kairo, Guillaume | Renault, David | Maibeche, Martine | Siaussat, David
Insecticides have long been used as the main method in limiting agricultural pests, but their widespread use has resulted in environmental pollution, development of resistances, and biodiversity reduction. The effects of insecticides at low residual doses on both the targeted crop pest species and beneficial insects have become a major concern. In particular, these low doses can induce unexpected positive (hormetic) effects on pest insects, such as surges in population growth exceeding what would have been observed without pesticide application. Methomyl and chlorpyrifos are two insecticides commonly used to control the population levels of the cotton leafworm Spodoptera littoralis, a major pest moth. The aim of the present study was to examine the effects of sublethal doses of these two pesticides, known to present a residual activity and persistence in the environment, on the moth physiology. Using a metabolomic approach, we showed that sublethal doses of methomyl and chlorpyrifos have a systemic effect on the treated insects. We also demonstrated a behavioral disruption of S. littoralis larvae exposed to sublethal doses of methomyl, whereas no effects were observed for the same doses of chlorpyrifos. Interestingly, we highlighted that sublethal doses of both pesticides did not induce a change in acetylcholinesterase activity in head of exposed larvae.
Afficher plus [+] Moins [-]Behavioral and metabolic effects of sublethal doses of two insecticides, chlorpyrifos and methomyl, in the Egyptian cotton leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae)
2016
Dewer, Youssef | Pottier, Marie-Anne | Lalouette, Lisa | Maria, Annick | Dacher, Matthieu | Belzunces, Luc | Kairo, Guillaume | Renault, David | Maibeche, Martine
Insecticides have long been used as the main method in limiting agricultural pests, but their widespread use hasresulted in environmental pollution, development of resistances, and biodiversity reduction. The effects of insecticides at low residual doses on both the targeted crop pest species and beneficial insects have become a major concern. In particular, these low doses can induce unexpected positive (hormetic) effects on pest insects, such as surges in population growth exceeding what would have been observed without pesticide application. Methomyl and chlorpyrifos are two insecticides commonly used to control the population levels of the cotton leafworm Spodoptera littoralis, a major pest moth. The aim of the present study was to examine the effects ofsublethal doses of these two pesticides, known to present a residual activity and persistence in the environment, on the moth physiology. Using a metabolomic approach, we showed that sublethal doses of methomyl and chlorpyrifos have a systemic effect on the treated insects. We also demonstrated a behavioral disruption of S. littoralis larvae exposed to sublethal doses of methomyl, whereas no effects were observed for the same doses of chlorpyrifos. Interestingly, we highlighted that sublethal doses of both pesticides did not induce a change in acetylcholinesterase activity in head of exposed larvae.
Afficher plus [+] Moins [-]Behavioral and metabolic effects of sublethal doses of two insecticides, chlorpyrifos and methomyl, in the Egyptian cotton leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae)
2016
Dewer, Youssef | Pottier, Marie-Anne | Lalouette, Lisa | Maria, Annick | Dacher, Matthieu | Belzunces, Luc | Kairo, Guillaume | Renault, David | Maibeche, Martine
Insecticides have long been used as the main method in limiting agricultural pests, but their widespread use hasresulted in environmental pollution, development of resistances, and biodiversity reduction. The effects of insecticides at low residual doses on both the targeted crop pest species and beneficial insects have become a major concern. In particular, these low doses can induce unexpected positive (hormetic) effects on pest insects, such as surges in population growth exceeding what would have been observed without pesticide application. Methomyl and chlorpyrifos are two insecticides commonly used to control the population levels of the cotton leafworm Spodoptera littoralis, a major pest moth. The aim of the present study was to examine the effects ofsublethal doses of these two pesticides, known to present a residual activity and persistence in the environment, on the moth physiology. Using a metabolomic approach, we showed that sublethal doses of methomyl and chlorpyrifos have a systemic effect on the treated insects. We also demonstrated a behavioral disruption of S. littoralis larvae exposed to sublethal doses of methomyl, whereas no effects were observed for the same doses of chlorpyrifos. Interestingly, we highlighted that sublethal doses of both pesticides did not induce a change in acetylcholinesterase activity in head of exposed larvae.
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