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Oxidative enzymatic response of white-rot fungi to single-walled carbon nanotubes
2014
Berry, Timothy D. | Filley, Timothy R. | Blanchette, R. A. (Robert A.)
Although carbon nanomaterials such as single-walled carbon nanotubes (SWCNT) are becoming increasingly prevalent in manufacturing, there is little knowledge on the environmental fate of these materials. Environmental degradation of SWCNT is hindered by their highly condensed aromatic structure as well as the size and aspect ratio, which prevents intracellular degradation and limits microbial decomposition to extracellular processes such as those catalyzed by oxidative enzymes. This study investigates the peroxidase and laccase enzymatic response of the saprotrophic white-rot fungi Trametes versicolor and Phlebia tremellosa when exposed to SWCNTs of different purity and surface chemistry under different growth conditions. Both unpurified, metal catalyst-rich SWCNT and purified, carboxylated SWCNTs promoted significant changes in the oxidative enzyme activity of the fungi while pristine SWCNT did not. These results suggest that functionalization of purified SWCNT is essential to up regulate enzymes that may be capable of decomposing CNT in the environment.
Afficher plus [+] Moins [-]Effects of salinity on anatomical features and physiology of a semi-mangrove plant Myoporum bontioides
2014
Xu, H.M. | Tam, N.F.Y. | Zan, Q.J. | Bai, M. | Shin, P.K.S. | Vrijmoed, L.L.P. | Cheung, S.G. | Liao, W.B.
The effect of different concentrations of NaCl, 0, 100, 200, 300 and 400mM, on the anatomical features and physiology of Myoporum bontioides was investigated. The photosynthetic rates (Pn) were significantly reduced by salt stress, with the lowest values at 400mM NaCl. The content of malondialdehyde (MDA), proline and soluble sugar, as well as the activities of peroxidase (POD) and catalase (CAT) increased at the beginning, but became similar to the control as the experiment proceeded. The NaCl effect on superoxide dismutase (SOD) was different from the other parameters, with a significant reduction at 400mM NaCl at Day 7. Salt glands were found in both upper and lower epidermis, and the ratios of the thickness of palisade to spongy mesophyll tissues increased with NaCl concentrations. The medullary ray was clearly damaged by NaCl at levels of 200 and 300mM. These results demonstrated that M. bontioides could adapt to a relatively low salinity, and was not a halophilous species.
Afficher plus [+] Moins [-]Effect of a polybrominated diphenyl ether congener (BDE-47) on growth and antioxidative enzymes of two mangrove plant species, Kandelia obovata and Avicennia marina, in South China
2014
The effects of BDE-47 on the growth and antioxidative responses of the seedlings of Kandelia obovata (Ko) and Avicennia marina (Am) were compared in an 8-week hydroponic culture spiked with different levels of BDE-47, 0.1, 1, 5 and 10mgl−1. The two highest BDE-47 levels significantly suppressed the growth and increased the activities of three antioxidative enzymes, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), of Ko in week 1. However, SOD and POD activities at high levels of BDE-47 became lower than the control in week 8. On the contrary, growth of Am was not affected at all contamination levels, and the activities of three enzymes were enhanced by BDE-47 in weeks 1 and 4, but such stimulatory effect became insignificant in week 8. Avicennia was more tolerant to BDE-47 toxicity than Kandelia, as its antioxidative enzymes could better counter-balance the oxidative stress caused by BDE-47.
Afficher plus [+] Moins [-]Irrigating Onions and Potatoes with Chromium and Nickel: Its Effects on Catalase and Peroxidase Activities and the Cross-Contamination of Plants
2014
Stasinos, Sotiris | Kostakis, Marios | Thomaidis, Nikolaos | Zabetakis, Ioannis
The scope of this study was to investigate the uptake of chromium and nickel by onions (Allium cepa) and potatoes (Solanum tuberosum) and their impact on plant enzymes catalase (CAT, E.C. 1.11.1.6) and peroxidase (POX, E.C. 1.11.1.7). A greenhouse experiment was conducted, simulating the irrigating conditions existing in the two biggest tuber-producing regions of Greece (Asopos and Messapia). Plants were cultivated for 4 months in six irrigation lines, each one supplied by an aqueous solution, containing levels of Cr(VI) and Ni(II) ranging from 0 μg/L (control) to 1,000 μg/L. Significant statistical correlations were observed between (i) the levels of heavy metals in plants, (ii) the levels of heavy metals in plants and in irrigation water, and (iii) the levels of heavy metals and the enzymatic activities in plants. The existing EU legislation has no legal limits for Ni and Cr in food, and the nutritional implications of this study are discussed.
Afficher plus [+] Moins [-]Effects of Perchlorate Stress on Growth and Physiological Characteristics of Rice (Oryza sativa L.) Seedlings
2014
Xie, Yinfeng | Tao, Gongsheng | Chen, Qian | Tian, Xueyao
Effects of perchlorate stress on the growth and physiological characteristics of rice (Oryza sativa L.) were studied in controlled water culture experiments. Perchlorate stress resulted in varied patterns of biomass allocation to O. sativa organs (roots, stems, and leaves). When stressed with higher perchlorate concentrations, the proportion of root biomass was higher, stem biomass was invariant, while leaf biomass was lower. Coefficients of variation in biomass of different organs followed the order leaf > stem > root, indicating that leaf growth has a higher sensitivity to perchlorate. Compared to the control, the chlorophyll and protein content of leaves and root vigor were lower, whereas malondialdehyde (MDA) content and catalase (CAT) activity were higher and related to perchlorate concentration and duration of stress. Superoxide dismutase (SOD) activity was initially high and then decreased markedly during the experiment, while peroxidase (POX) activity in perchlorate-treated rice was always higher than the POX activity of the control. POX was the most sensitive antioxidant enzyme to perchlorate stress. Correlation analysis showed a positive correlation between SOD activity and the fresh weight of the whole plant, and negative correlation with MDA content. The results suggest that perchlorate could induce oxidative stress and oxidative damage may be the main cause of physiological damage and growth inhibition in rice plants under perchlorate stress.
Afficher plus [+] Moins [-]Phytostabilization and Physicochemical Responses of Korean Ecotype Solanum nigrum L. to Cadmium Contamination
2014
Khan, Abdur Rahim | Ullah, Ihsan | Khan, Abdul Latif | Hong, Sung-Jun | Waqas, Muhammad | Park, Gun-Seok | Kwak, Yunyoung | Choi, JungBae | Jung, Byung-Kwon | Park, Man | Lee, In-Jung | Shin, Jae-Ho
Plants of the black nightshade (Solanum nigrum L.) Korean ecotype were exposed to a gradient of cadmium (Cd) concentrations (0, 10, 30, 50, and 80 mg kg⁻¹of dry sand). The results showed a significant (p < 0.05) reduction in biomass, root-shoot length, and chlorophyll contents in the plants exposed to Cd compared to the control. Cd concentrations significantly increased in different parts of the plants as indicated by inductively coupled plasma mass spectrometry (ICP-MS) analysis. The amount of Cd accumulated by the plants in the leaves, stems, and roots was 307, 1536, and 3163 mg kg⁻¹of dry matter, respectively, when treated with Cd 80 mg kg⁻¹. The translocation factor (TF) declined with higher Cd concentrations, whereas the bioconcentration factor (BCF) increased with elevated Cd levels. The response to oxidative stress induced by Cd was modulated by the enzymatic activity of peroxidase and polyphenol peroxidase. In terms of non-enzymatic antioxidant biochemicals such as reduced glutathione and polyphenols, its contents in the leaves significantly increased in a dose-dependent manner. The overall increased antioxidant defense response in leaves might have contributed to the higher accumulation and tolerance of plants against Cd-induced oxidative stress. The Korean ecotype of S. nigrum has potential phytoremediation utility for phytostabilization of Cd-contaminated marginal land. However, further genomic insights could contribute to the identification of potential Cd translocation genes.
Afficher plus [+] Moins [-]Ecotoxicological effects on the earthworm Eisenia fetida following exposure to soil contaminated with imidacloprid
2014
Zhang, Qingming | Zhang, Baohua | Wang, Caixia
Imidacloprid, a neonicotinoid insecticide, has been used widely in agriculture worldwide. The adverse effects of imidacloprid on exposed biota have brought it increasing attention. However, knowledge about the effects of imidacloprid on antioxidant defense systems and digestive systems in the earthworm is vague and not comprehensive. In the present study, the changes in the activity of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), cellulase, reactive oxygen species (ROS), and malondialdehyde (MDA) in the earthworm Eisenia fetida exposed to artificial soil treated with imidacloprid were examined systematically. The results showed that the activity of these biomarkers was closely related to the dose and duration of the exposure to imidacloprid. The activity of SOD was stimulated significantly at doses of 0.66 and 2 mg kg⁻¹ imidacloprid but markedly inhibited at a dose of 4 mg kg⁻¹ imidacloprid with prolonged exposure. The activities of CAT and POD increased irregularly at 0.2–4 mg kg⁻¹ imidacloprid over different exposure times. The level of ROS at a dose of 2 or 4 mg kg⁻¹ imidacloprid was significantly increased over the entire exposure period. When the concentration of imidacloprid was above 0.66 mg kg⁻¹, the balance of the activity of the antioxidant enzymes and ROS level was interrupted. The activity of cellulase decreased significantly with prolonged exposure. At the stress of 4 mg kg⁻¹ imidacloprid, the content of MDA was significantly increased with increasing exposure time. The results of the present study suggest that imidacloprid has a potentially harmful effect on E. fetida and may be helpful for assessment of the risk of imidacloprid to the soil ecosystem environment. However, to obtain more comprehensive toxicity data, it is necessary to investigate the effects of imidacloprid on earthworm using native soils in the future work.
Afficher plus [+] Moins [-]Use of hairy roots extracts for 2,4-DCP removal and toxicity evaluation by Lactuca sativa test
2014
Angelini, Vanina A. | Agostini, Elizabeth | Medina, María I. | González, Paola S.
2,4-Dichlorophenol (2,4-DCP) is widely distributed in wastewaters discharged from several industries, and it is considered as a priority pollutant due to its high toxicity. In this study, the use of different peroxidase extracts for 2,4-DCP removal from aqueous solutions was investigated. Tobacco hairy roots (HRs), wild-type (WT), and double-transgenic (DT) for tomato basic peroxidases (TPX1 and TPX2) were used to obtain different peroxidase extracts: total peroxidases (TPx), soluble peroxidases (SPx), and peroxidases ionically bound to the cell wall (IBPx). All extracts derived from DT HRs exhibited higher peroxidase activity than those obtained from WT HRs. TPx and IBPx DT extracts showed the highest catalytic efficiency values. The optimal conditions for 2,4-DCP oxidation were pH 6.5, H₂O₂0.5 mM, and 200 U mL⁻¹of enzyme, for all extracts analyzed. Although both TPx extracts were able to oxidize different 2,4-DCP concentrations, the removal efficiency was higher for TPx DT. Polyethylene glycol addition slightly improved 2,4-DCP removal efficiency, and it showed some protective effect on TPx WT after 2,4-DCP oxidation. In addition, using Lactuca sativa test, a reduction of the toxicity of post removal solutions was observed, for both TPx extracts. The results demonstrate that TPx extracts from both tobacco HRs appear to be promising candidate for future applications in removing 2,4-DCP from wastewaters. This is particularly true considering that these peroxidase sources are associated with low costs and are readily available. However, TPx DT has increased peroxidase activity, catalytic efficiency, and higher removal efficiency than TPx WT, probably due to the expression of TPX1 and TPX2 isoenzymes.
Afficher plus [+] Moins [-]Oxidative injury and antioxidant genes regulation in cadmium-exposed radicles of six contrasted Medicago truncatula genotypes
2014
Rahoui, Sondès | Ben, Cécile | Chaoui, Abdelilah | Martinez, Yves | Yamchi, Ahad | Rickauer, Martina | Gentzbittel, Laurent | El Ferjani, Ezzeddine
Oxidative disorders were triggered in the presence of Cd toxicity in early seedling growth of six Medicago truncatula genotypes. Interactions between root growth inhibition, cadmium uptake, as well as the occurrence of oxidative injury suggest differential responses of the genotypes, with susceptible or tolerant accessions. ROS enhancement was observed in situ and H₂O₂ content was measured, that did not seem related to tolerance or susceptibility. Oxidative burst impact on cell membrane integrity was analyzed in agreement with MDA content and glucose exudation, which suggest an active role of this burst in susceptible lines. Transcriptional changes in response to cadmium treatment were analyzed on target genes involved in (1) ROS-scavenging enzymes (superoxide dismutase (SOD; EC1.15.1.1) and peroxidase (PRX; EC 1.11.1.7)), (2) reduced glutathione (γ-Glu-Cys-Gly, GSH) metabolism (glutathione-S-transferase (GST; EC: 2.5.1.18) and glutathione reductase (GR; EC 1.8.1.7)), and (3) metal-chelating metabolism (PCS). The susceptible line shows no response or non-timely gene expression patterns. This research work gave an overview of the deleterious effects and oxidative injury of cadmium stress in Medicago truncatula. Oxidative defense efficiency and gene upregulation should explain relative tolerance in tested genotypes.
Afficher plus [+] Moins [-]Anatomical and physiological responses of Colorado blue spruce to vehicle exhausts
2014
Qin, Xuebo | Sun, Nan | Ma, Lixin | Chang, Yingqiao | Mu, Liqiang
In order to examine whether the leaves of the Colorado blue spruce (Picea pungens) are damaged or not by traffic pollution, the traits of the anatomy and physiology of its leaves are investigated by exposure to vehicle exhausts in a laboratory experiment lasting 30 days. The results show that both the anatomical structures and physiological traits of the leaves are significantly affected by vehicle exhausts. The anatomical structures, including epidermis, cuticle, palisade, and spongy parenchyma are modified when exposed to the high concentrations (≥0.4 mg/m³) of vehicle exhausts. However, physiological traits such as total chlorophyll content are not changed when exposed to different concentrations of vehicle exhaust. Unlike the total chlorophyll content, the electrical conductivities increased, whereas the POD activities decreased when presented in vehicle exhausts. The present study indicates that the Colorado blue spruce changes its anatomical structures and physiological traits to avoid possible damage by vehicle exhausts.
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