Currently little is known of newer pesticide classes and their occurrence and persistence in recreational lakes. Therefore, the objectives of this study were to (1) assess average pesticide concentrations and loadings entering recreational lakes in three mixed land use watersheds throughout the growing season, (2) evaluate pesticide persistence longitudinally within the lakes, and (3) perform an ecotoxicity assessment. Six sampling campaigns were conducted at three lakes from April through October 2018 to measure the occurrence and persistence during pre, middle, and post growing season. Polar organic chemical integrative samplers (POCIS) were placed in streams near lake inlets and monthly samples were collected for analysis of twelve pesticides. Additional monthly grab water samples were taken at each POCIS location and at the midpoint and outlet of each lake. All pesticide samples were analyzed using liquid chromatography/tandem mass spectrometry (LC/MS/MS) and individual pesticide loading rates were determined. Occurrence and persistence of specific pesticides were significantly different between lakes in varying watershed land uses. Specifically, the recreational lake receiving predominately urban runoff had the highest load of pesticides, likely in the form of biocides, entering the waterbody. Concentrations of imidacloprid exceeded acute and chronic invertebrate levels for 11% and 61% of the sampling periods, respectively, with the recreational lake receiving predominately urban runoff having the most occurrences. Findings from this study are critical for preventing and mitigating potential effects of pesticides, specifically applied as biocides in urban landscapes, from entering and persisting in recreational lakes.

Twenty-two years after the last application of ring-¹⁴C-labeled atrazine at customary rate (1.7 kg ha⁻¹) on an agriculturally used outdoor lysimeter, atrazine is still detectable by means of accelerated solvent extraction and LC-MS/MS analysis. Extractions of the 0-10 cm soil layer yielded 60% of the residual ¹⁴C-activity. The extracts contained atrazine (1.0 μg kg⁻¹) and 2-hydroxy-atrazine (42.5 μg kg⁻¹). Extractions of the material of the lowest layer 55-60 cm consisting of fine gravel yielded 93% of residual ¹⁴C-activity, of which 3.4 μg kg⁻¹ was detected as atrazine and 17.7 μg kg⁻¹ was 2-hydroxy-atrazine. The detection of atrazine in the lowest layer was of almost four times higher mass than in the upper soil layer. These findings highlight the fact that atrazine is unexpectedly persistent in soil. The overall persistence of atrazine in the environment might represent a potential risk for successive groundwater contamination by leaching even after 22 years of environmental exposure. Atrazine and its metabolite 2-hydroxy-atrazine are still present in soil after long-term aging.

The biomixture composition and the presence of a grass layer in a biobed bioremediation system can improve the performance of these systems to minimize pesticide point-source contamination. In this study, a novel biomixture composed with organic wastes from vineyards and wine industries (vermicompost of winery wastes and vine shoots) and top soil (W) was elaborated. The impact of three pesticides, commonly used in vineyards, on its microbial activity and on the development of turfgrass was determined in a short-term experiment. Moreover, the dissipation of the assayed pesticides was evaluated to stablish their distribution patterns between the turfgrass and the biomixture. For comparison, the original biomixture composed with top soil, peat, and straw (P) was also studied. After 15 days of pesticide application, the development of the turfgrass in both biomixtures was similar. However, the oxidoreductases (dehydrogenase and ortho-diphenol oxidase) and the hydrolytic (FDA and β-glucosidase) enzyme activities were greater in W-biomixture than in P-biomixture. The dissipation of metalaxyl and imidacloprid recorded in the W-biomixtures was significantly greater than in the P-biomixtures. The pesticide dissipation in W-biomixtures followed the same order of their octanol water partition coefficients. Except for tebuconazole, the lower biological activity in the P-biomixture would explain the limited pesticide dissipation. In the grassed biomixtures, most (> 83%) of the non-dissipated imidacloprid and tebuconazole remained in the biomixtures, while metalaxyl was rapidly translocated to the aerial part of the turfgrass. Our results show the potential capability of the novel biomixture as an alternative to the original one in a biobed.

An experimental study to investigate the potential soil accumulation of the triazole fungicide difenoconazole in soil was carried out in northwestern Italy. The fungicide was applied to sugar beet for 4 years with three applications per year at a rate of 75 g ha⁻¹ each, according to formulated product recommended use. Soil cores were collected each year before the first application, after each application and at harvest of the crop. The soil samples were then split into 0-10 and 10-40 cm depth layers, extracted and quantitatively analysed by gas chromatography for difenoconazole residues. The study evidenced that difenoconazole residues could be detected in the upper soil layer only, in quantities detectable after several applications (0.14 to 0.32 mg kg⁻¹ after the third application) which then become undetectable the following year. It can be concluded, therefore, that difenoconazole does not accumulate in soil.

To understand the influence of Cd on atrazine (ATZ) degradation in aqueous solution, the degradation of different initial levels of ATZ (0.1, 0.5, 1.0, and 2.0 mg·L⁻¹) was investigated in the presence and absence of Cd²⁺ in a 20-day laboratory experiment. It was found that Cd²⁺ caused a significant decrease in ATZ degradation and increased its half-life from 17–34 days to 30–57 days (p < 0.0001). Regarding the three most common metabolites of ATZ, deethylatrazine (DEA) and deisopropylatrazine (DIA) were detected in water earlier than hydroxyatrazine (HYA). The DEA content was several times higher than the DIA and HYA contents, regardless of the presence or absence of Cd²⁺. In the presence of Cd²⁺, the DIA content was significantly lower and the HYA content was significantly higher. Furthermore, Cd²⁺ had a dose-dependent effect on HYA formation. Our results indicated that the coexistence of Cd²⁺ and ATZ resulted in greater herbicide persistence, thereby possibly increasing the risk of environmental contamination. DEA was still the predominant ATZ degradation product detected in water under the combined pollution, which was similar to the ATZ tendency.

Stormwater wetlands collect and attenuate runoff-related herbicides, limiting their transport into aquatic ecosystems. Knowledge on wetland bacterial communities with respect to herbicide dissipation is scarce. Previous studies showed that hydrological and hydrochemical conditions, including pesticide removal capacity, may change from spring to summer in stormwater wetlands. We hypothesized that these changes alter bacterial communities, which, in turn, influence pesticide degradation capacities in stormwater wetland. Here, we report on bacterial community changes in a stormwater wetland exposed to pesticide runoff, and the occurrence of trz, atz, puh, and phn genes potentially involved in the biodegradation of simazine, diuron, and glyphosate. Based on T-RFLP analysis of amplified 16S rRNA genes, a response of bacterial communities to pesticide exposure was not detected. Changes in stormwater wetland bacterial community mainly followed seasonal variations in the wetland. Hydrological and hydrochemical fluctuations and vegetation development in the wetland presumably contributed to prevent detection of effects of pesticide exposure on overall bacterial community. End point PCR assays for trz, atz, phn, and puh genes associated with herbicide degradation were positive for several environmental samples, which suggest that microbial degradation contributes to pesticide dissipation. However, a correlation of corresponding genes with herbicide concentrations could not be detected. Overall, this study represents a first step to identify changes in bacterial community associated with the presence of pesticides and their degradation in stormwater wetland.

This study is based on self-reported information collected from selected farmers of Vehari District, Punjab, Pakistan, to determine their technical knowledge and awareness about pesticide use and associated environmental and health risks. Moreover, soil contamination by routinely used persistent organochlorine pesticide, endosulfan, was also evaluated. Survey data revealed very low literacy rate (on an average 9th grade education) and technical knowledge (almost missing) of the farmers in Vehari District. The farmers are unable to fully read and understand the instructions about the use of pesticide marked on the containers. They are not fully aware of pesticide persistence and toxicity (73%), unable to identify cotton pests and diseases (86%), and do not know which crop to grow in cotton adjacent fields (100%). Data also revealed that the farmers (100%) do not follow safety measure during pesticide application and are unaware of pesticide toxicity symptoms in human as well as the basic first-aid practices (89%).Poor literacy rate and lack of technical knowledge of farmers in Vehari regarding pesticide use and handling are posing serious environmental and health risks among the local inhabitants, particularly among farmers. Soil analysis results showed that concentration of α- and β-endosulfan ranged from 0–14 to 0–14.64 μg/mg, respectively. Principal component analysis showed that soil organic matter is the key soil parameter controlling the occurrence and fate of endosulfan under sandy loam soil conditions of Vehari District. There is a serious need of improving technical and environmental knowledge of farmer about pesticide risks on human health in the studied area, in particular, and the entire country in general. Findings are of great use for policymaking in Pakistan to minimize pesticide risks in Pakistan.

Microbial community compositions in pesticide-contaminated aquifers have not been studied, although such information is important for remediation and maintaining freshwater sources clean under changing climate. Therefore, phospholipid (PLFAs), glycolipid (GLFAs), and neutral lipid (NLFAs) fatty acids were determined from sand and clay sediments at depths of 0.3–24.8 m, all contaminated with triazines and dichlobenil/2,6-dichlorobenzamide. The portion of fungi and Gram-negative bacteria at 0.3 m was greater than at 0.8 m, where the percentage of Gram-positive bacteria, actinobacteria, and sulfate-reducing bacteria (SRB) increased. In deeper sediments, microbial biomass, activity, and diversity decreased. Clay sediments seemed to serve as a reservoir for slow pesticide elution to groundwater, and their biomarker portion for all bacteria except actinobacteria was greater than in sand sediments. The slow pesticide dissipation seemed to occur in the main groundwater flow zone, resulting in nitrogen release simultaneously with organic matter elution from gardening and bank filtration. As a result, microbial biomass, activity, and diversity were increased. This shift in conditions towards that in surface soil may be appropriate for enhanced natural attenuation of pesticides in groundwater sources.

Misuse of pesticides in farming activities leads to contamination of drinking water sources and is responsible for animal and human health problems. The biobeds are practicable option to minimize contamination by pesticides during preparation, use and washing of equipment for pesticide treatments. This research aimed at testing substrate mixtures to optimize biobed efficiency to remove pesticides under the climate of the Yucatan (México). Agricultural soil and 11 mixtures adding vegetable compost, sisal pulp, corn stover and seaweed were tested under controlled conditions. Each biomixture was exposed to a mixture of five pesticides (2,4-diclorophenoxyacetic acid “2,4-D” [1.08 mg cm⁻³], atrazine [2.50 mg cm⁻³], carbofuran [0.23 mg cm⁻³], diazinon [0.34 mg cm⁻³], and glyphosate [0.36 mg cm⁻³]) in a period of 41 days. Monitoring of the dissipation of pesticide residues showed that pesticides were quickly dissipated in soil at microcosm level experiment, while at two critical times of 20 and 41 days, all mixtures of substrates (biomixtures) were efficient in dissipation of high concentrations of pesticide in a short time (>99%). Time, biomixture and type of pesticide were shown to be the main parameters influencing pesticide dissipation (P < 0.05). Several other physicochemical parameters of the biomixtures, such as organic matter (OM), lignin, water holding capacity (WHC), and pH, were also significant on pesticide dissipation (P < 0.05), being pH the most significant.

In 2009, hexachlorocyclohexane (HCH) isomers (α-HCH, β-HCH, and γ-HCH [lindane]) were listed as persistent organic pollutants (POP) in the Stockholm Convention. Accordingly, the legacy of HCH/lindane production with the associated large HCH waste deposits has become recognized as an issue of global concern and is addressed in the implementation of the Convention. The current paper gives an overview of the major contaminated sites from lindane production of the INQUINOSA Company. This company operated from 1975 to 1988 in the city of Sabiñánigo, Spain. HCH production resulted in the production of approximately 115,000 tonnes of waste isomers which were mainly dumped in two unlined landfills. These two landfill sites, together with the former production site, are recognized sources of environmental pollution. Assessment and remediation activities at these sites are described. A dense nonaqueous phase liquid (DNAPL) contaminated inter alia with HCH isomers, benzene, chlorobenzenes, and chlorophenols as the main contaminants and an associated contaminated groundwater plume have been discovered at both landfill/dumpsites and at the former production site. The approximately 4,000 t of DNAPLs constitute a serious risk for the environment due to the proximity of the Gállego River. Since 2004, more than 20 tonnes of this DNAPL has been extracted using “pump and treat” techniques. The Aragon Regional Government and the Spanish Environmental Ministry are taking action, focusing on the treatment of DNAPL and on the transfer of the large quantities of solid POP wastes to a new landfill. A range of laboratory tests has been performed in order to plan the aquifer remediation.