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Source tracing with cadmium isotope and risk assessment of heavy metals in sediment of an urban river, China
2022
Fang, Ding | Wang, Hui | Liang, Yangyang | Cui, Kai | Yang, Kun | Lu, Wenxuan | Li, Jing | Zhao, Xiuxia | Gao, Na | Yu, Qizhi | Li, Hui | Jiang, He
The Nanfei River was one of dominant inflowing rivers of the fifth largest freshwater Chaohu Lake in China, which had been subjected to increasing nutrients and contaminants from population expansion, rapid industrialization and agricultural intensification in recent decades. In present study, surface sediment from the Nanfei River was collected to investigate the anthropogenic impact on distribution and bioavailability of heavy metals. Possible Cd sources along the river were constrained by using Cd isotope signatures and labile concentrations of heavy metals in sediment were determined through the DGT technique for risk assessment. Results showed that Cd in river sediment showed greatest enrichment (EF 0.8–9.4), indicating massive pollution from anthropogenic activities. Among the various possible Cd source materials, urban road dust, industrial soil and chicken manure, displayed higher Cd abundance and enrichment that might contribute to Cd accumulation in river sediment. Cadmium isotopic composition in river sediment was ranged from −0.21 ± 0.01‰ to 0.13 ± 0.03‰, whereas yielded relative variation from −0.31 ± 0.02‰ to 0.23 ± 0.01‰ in source materials. Accordingly, Cd sources along the river were constrained, i.e. traffic and industrial activities in the upper and middle reaches whereas agricultural activities in the lower reaches. Furthermore, the evaluation on ecological risk of heavy metals in sediment on basis of SQGs and DGT-labile concentrations demonstrated that Pb and Zn might pose higher risk on aquatic species. The present study confirmed that Cd isotopes were promising source tracer in environmental studies.
Afficher plus [+] Moins [-]Reducing environmental risks of chlorpyrifos application in typical soils by adding appropriate exogenous organic matter: Evidence from a simulated paddy field experiment
2022
Shen, Dahang | Yu, Kaixiang | Hu, Jirong | Zhong, Jiayin | Shen, Guoqing | Ye, Qingfu | Wang, Wei
Chlorpyrifos (CPF), as an organophosphate insecticide extensively used in the modern agricultural system, has been gradually banned in many countries due to its reported health risks to organisms, including humans. This study used simulated paddy field experiments and carbon-14 tracing to explore the possibility of reducing environmental risks of chlorpyrifos application through appropriate agronomic practice. Results showed ¹⁴C-CPF concentration in rice plants planted in the red soil (RS) was significantly higher than that in black soil (BS) and fluvo-aquic soil (FS). The application of biochar and chicken manure in RS reduced ¹⁴C-CPF accumulation in rice plants, and the content of ¹⁴C-CPF in rice grains decreased by 25% and 50%, respectively. Adding biochar to all three soils reduced the migration of ¹⁴C-CPF, especially in FS with the highest risk of ¹⁴C-CPF migration. The addition of chicken manure in FS reduced the migration of ¹⁴C-CPF and the total residual amount of ¹⁴C-CPF in the soil. In addition, chicken manure treatment increased the formation of ¹⁴C-bound residues (BRs) in soils and changed the distribution ¹⁴C-BRs in humus. The results indicated that the degree of environmental risks associated with the CPF application varies with soil types and could be reduced by introducing suitable exogenous organic matter into different soils, which is of great significance for guiding the scientific application of chlorpyrifos in agronomic practices.
Afficher plus [+] Moins [-]Impact of different manure-derived dissolved organic matters on the fate of arsenic-antibiotic in co-contaminated paddy soils
2022
Yan, Mengmeng | Zhu, Changxiong | Song, Tingting | Li, Binxu | Su, Shiming | Li, Hongna
Manure application increases the transfer risk of antibiotic resistance to farmland. Especially, its impact remains unclear when it occurs in arsenic (As)-contaminated paddy soils, which is considered as a global environmental problem. In this work, we investigated the fate of antibiotic resistance genes (ARGs) in As-antibiotic co-contaminated paddy soils under the application of manure from different sources (pig manure, cow dung, and chicken manure). Differences in the aliphatic carbon and electron-donating capacities of these dissolved organic matters (DOM) regulated the transformation of iron and As by both biotic and abiotic processes. The regulation by pig manure was stronger than that by cow dung and chicken manure. DOM regulation increased the abundance of As-related functional genes (arsC, arrA, aioA, and arsM) in the soil and accelerated the transformation of As speciation, the highest proportion of As(III) being 45%–61%. Meanwhile, the continuous selection pressure provided by the highly toxic As(III) increased the risk of ARGs and mobile genetic elements (MGEs) via horizontal gene transfer. As-resistant bacteria, including Bacillus, Geobacter, and Desulfitobacterium, were finally considered as potential host bacteria for ARGs and MGEs. In summary, this study clarified the synergistic mechanism of As-antibiotic on the fate of ARGs in co-contaminated paddy soils, and provided practical guidance for the proper application of organic fertilizers.
Afficher plus [+] Moins [-]Occurrence of microplastic in livestock and poultry manure in South China
2021
Wu, Rui-Ting | Cai, Ying-Feng | Chen, Ying-Xi | Yang, Yi-Wen | Xing, Si-Cheng | Liao, Xin-Di
Microplastic (MP) contamination in soil has attracted much attention, and increasing evidence suggests that MPs can accumulate in agricultural soils through fertilization by compost. In addition, the most common raw materials for composting are livestock and poultry manure wastes. Because the presence of MPs may threaten the safe utilization of fertilizers composted by livestock and poultry wastes during crop planting, it is necessary to understand the contamination risk of MPs present in livestock and poultry manure. In this study, the distribution of MPs in 19 livestock and poultry farms with 3 different species was investigated by using FTIR microscopy. A total of 115 items manure MPs and 18 items feed MPs were identified as PP and PE types dominated by colorful fragments and fibers. Furthermore, after comparing the compositions of plastic products used in the feeding process, we proposed two transport pathways for MP pollution in manure and one potential transport pathway in feeds. Our result proved that the application of swine and poultry manure directly could be a new route of MPs in agricultural soil, furthermore, the presence of MPs could threaten the safety of resource utilization in agricultural soil by using swine and poultry manure for manure potentially. Not, only that, our study also provided a reference for the remediation of MP-contaminated soil.
Afficher plus [+] Moins [-]Effects of multi-year biofumigation on soil bacterial and fungal communities and strawberry yield
2020
Zhang, Daqi | Yan, Dongdong | Cheng, Hongyan | Fang, Wensheng | Huang, Bin | Wang, Xianli | Wang, Xiaoning | Yan, Yue | Ouyang, Canbin | Li, Yuan | Wang, Qiuxia | Cao, Aocheng
Biofumigation is an effective, non-chemical method to control soil-borne pests and diseases and to maximize crop yield. We studied the responses of soil bacterial and fungal communities, the soil’s nutritional state and strawberry yield, when the soil was biofumigated each year for five consecutive years using fresh chicken manure (BioFum). BioFum significantly increased the soil’s NH4+-N, NO3−-N, available P and K and organic matter. Fusarium spp. and Phytophthora spp. which are known to cause plant disease, were significantly decreased after BioFum. In addition, Biofum increased the soil’s temperature, enhanced chlorophyll levels in the leaves of strawberry plants, and the soluble sugar and ascorbic acid content in strawberry fruit. We used high-throughput gene sequencing to monitor changes in the soil’s bacterial and fungal communities. Although BioFum significantly decreased the diversity of these communities, it increased the relative abundance of some biological control agents in the phylum Actinobacteria and the genera Pseudomonas, Bacillus and Chaetomium. An increase in these biological control agents would reduce the incidence of soil-borne pathogens and plant disease. Although strawberry marketable yield using BioFum was higher in the first three years, the decline in the final two years could be due to the accumulation of P and K which may have delayed flowering and fruiting. Methods to overcome yield losses using BioFum need to be developed in the future. Our research, however, showed that BioFum enhanced soil fertility, reduced the presence of soil pathogens, increased the relative abundance of beneficial bacteria and fungi and improved strawberry quality. Unlike chemical soil treatments that can cause pest and disease resistance when used continuously over many years, our multi-year research program on BioFum showed that this treatment provided significant benefits to the soil, plant and strawberry fruit.
Afficher plus [+] Moins [-]Impacts of different sources of animal manures on dissemination of human pathogenic bacteria in agricultural soils
2020
Li, Jinyang | Chen, Qinglin | Li, Helian | Li, Shiwei | Liu, Yinghao | Yang, Liyuan | Han, Xuemei
The human pathogenic bacteria (HPB) in animal feces may disseminate to agricultural soils with their land application as organic fertilizer. However, the knowledge about the impacts of different sources and rates of animal manures on the temporal changes of soil HPB remains limited, which hamper our ability to estimate the potential risks of their land application. Here, we constructed an HPB database including 565 bacterial strains. By blasting the 16 S rRNA gene sequences against the database we explored the occurrence and fate of HPB in soil microcosms treated with two rates of swine, poultry or cattle manures. A total of 30 HPB were detected in all of manure and soil samples. Poultry manure at the high level obviously improved the abundance of soil HPB. The application of swine manure could introduce concomitant HPB into the soils. Of which, Pseudomonas syringae pv. syringae B728a and Escherichia coli APEC O78 may deserve more attention because of their survival for a few days in manured soils and being possible hosts of diverse antibiotic resistance genes (ARGs) as revealed by co-occurrence pattern. Bayesian source tracking analysis showed that the HPB derived from swine manure had a higher contribution to soil pathogenic communities than those from poultry or cattle manures in early days of incubation. Mantel test together with variation partitioning analysis suggested that bacterial community and soil physicochemical properties were the dominant factors determining the profile of HPB and contributed 64.7% of the total variations. Overall, our results provided experimental evidence that application of animal manures could facilitate the potential dissemination of HPB in soil environment, which should arouse sufficient attention in agriculture practice and management to avoid the threat to human health.
Afficher plus [+] Moins [-]Biotransformation of arsenic-containing roxarsone by an aerobic soil bacterium Enterobacter sp. CZ-1
2019
Huang, Ke | Peng, Hanyong | Gao, Fan | Liu, Qingqing | Lu, Xiufen | Shen, Qirong | Le, X Chris | Zhao, Fang-Jie
Roxarsone (3-nitro-4-hydroxyphenylarsonic acid, ROX) is an arsenic-containing compound widely used as a feed additive in poultry industries. ROX excreted in chicken manure can be transformed by microbes to different arsenic species in the environment. To date, most of the studies on microbial transformation of ROX have focused on anaerobic microorganisms. Here, we isolated a pure cultured aerobic ROX-transforming bacterial strain, CZ-1, from an arsenic-contaminated paddy soil. On the basis of 16S rRNA gene sequence, strain CZ-1 was classified as a member of the genus Enterobacter. During ROX biotransformation by strain CZ-1, five metabolites including arsenate (As[V]), arsenite (As[III]), N-acetyl-4-hydroxy-m-arsanilic acid (N-AHPAA), 3-amino-4-hydroxyphenylarsonic acid (3-AHPAA) and a novel sulfur-containing arsenic species (AsC₉H₁₃N₂O₆S) were detected and identified based on high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS), HPLC-ICP-MS/electrospray ionization mass spectrometry (ESI-MS) and HPLC-electrospray ionization hybrid quadrupole time-of-flight mass spectrometry (ESI-qTOF-MS) analyses. N-AHPAA and 3-AHPAA were the main products, and 3-AHPAA could also be transformed to N-AHPAA. Based on the results, we propose a novel ROX biotransformation pathway by Enterobacter. sp CZ-1, in which the nitro group of ROX is first reduced to amino group (3-AHPAA) and then acetylated to N-AHPAA.
Afficher plus [+] Moins [-]Using column experiments to examine transport of As and other trace elements released from poultry litter: Implications for trace element mobility in agricultural watersheds
2017
Oyewumi, Oluyinka | Schreiber, Madeline E.
Trace elements are added to poultry feed to control infection and improve weight gain. However, the fate of these trace elements in poultry litter is poorly understood. Because poultry litter is applied as fertilizer in many agricultural regions, evaluation of the environmental processes that influence the mobility of litter-derived trace elements is critical for predicting if trace elements are retained in soil or released to water. This study examined the effect of dissolved organic carbon (DOC) in poultry litter leachate on the fate and transport of litter-derived elements (As, Cu, P and Zn) using laboratory column experiments with soil collected from the Delmarva Peninsula (Mid-Atlantic, USA), a region of intense poultry production. Results of the experiments showed that DOC enhanced the mobility of all of the studied elements. However, despite the increased mobility, 60–70% of Zn, As and P mass was retained within the soil. In contrast, almost all of the Cu was mobilized in the litter leachate experiments, with very little retention in soil. Overall, our results demonstrate that the mobility of As, Cu, Zn and P in soils which receive poultry litter application is strongly influenced by both litter leachate composition, specifically organic acids, and adsorption to soil. Results have implications for understanding fate and transport of trace elements released from litter application to soil water and groundwater, which can affect both human health and the environment.
Afficher plus [+] Moins [-]Characteristics of oxytetracycline stress-sensitive microbe-dissolved organic matter component interactions during composting
2022
Zhang, Xu | Zhang, Xinlin | Cui, Hongyang | Zhao, Ran | Zhao, Meiyang | Wei, Zimin
Dissolved organic matter (DOM) has important impacts on the transportation of antibiotics through chemical and biological processes in composting. The interaction between DOM and antibiotics is reciprocal. The interaction between DOM ligands and antibiotics could be characterized based on a technique combining parallel factor analysis (PARAFAC) and microbial community structure analysis. However, PARAFAC cannot reveal the dynamic changes in each DOM peak in one PARAFAC component under antibiotic stress. In this study, two-dimensional correlation spectroscopy (2DCOS) combined with PARAFAC and bacterial community diversity analyses were employed to reveal the effects of oxytetracycline (OTC) stress and the key microorganisms on the transformation of different fluorescent peaks from DOM PARAFAC components during chicken manure composting. The results showed that OTC inhibits the transformation between DOM PARAFAC components by inhibiting the core microbial activities involved in the transformation of DOM components. Protein-like components (C1 and C2) were more sensitive to OTC residue, and components with a high humification degree promoted the degradation of OTC. The interaction between special DOM PARAFAC components and certain bacteria affects the degradation of OTC. The DOM PARAFAC components A2(C1), B1(C2), B2(C2) and Z1(C4) enhanced OTC degradation by stimulating the genera Pseudomonas, Glycomyces and Hyphomicrobium. With these promising results, the true effect of DOM PARAFAC components on the degradation of OTC can be revealed, which is helpful for addressing antibiotic contamination to improve the bioavailability of compost products.
Afficher plus [+] Moins [-]Influence of humic acid structure on the accumulation of oxyfluorfen in tropical soils of mountain agroecosystems
2021
Barbosa, Daniele Rodrigues | García, Andrés Calderín | da Costa Barros de Souza, Camila | Amaral Sobrinho, Nelson Moura Brasil do
Herbicide application is a practice commonly used in agricultural systems because it is an efficient method of weed control. An inherent characteristic of some herbicides used in mountain agriculture, such as oxyfluorfen, is high adsorption to soil organic matter (SOM). Thus, intensive management that changes the quantity and quality of SOM, such as soil tillage and the massive application of organic fertilizers such as poultry litter, may favor soil contamination by this herbicide and alter its dynamics in the environment. Therefore, this study aimed to characterize the structures of humic substances (HSs) in the soil of forest areas and areas with intensive production of vegetables, relating them to the accumulation of the herbicide oxyfluorfen in tropical mountain agroecosystems. Organic carbon content was quantified in HSs, humic acid (HAs) were structurally characterized by CP/MAS ¹³C-NMR spectroscopy, and the oxyfluorfen molecule was detected and quantified using the QuEChERS residue detection method with subsequent analysis by LC-MS/MS. Oxyfluorfen was not detected in the forest areas, but it was detected in the vegetable growing areas at points with the lowest slope and high contents of organic matter and clay, with values of up to 0.13 mg kg⁻¹. The intensification in the SOM mineralization process, promoted by the intensive management adopted in the vegetable growing areas, resulted in a 16.46% reduction in COT, a 58.84% reduction in the carbon content in the form of SH and a reduction in the structures that give recalcitrance to the HA molecule (CAₗₖyₗ-H,R, CCOO–H,R, CAᵣₒₘₐₜᵢc-H,R, and CAᵣₒₘₐₜᵢc-O) when compared to those values in the forest area, presenting HAs with more aliphatic and labile properties. Thus, due to the structural characteristics of the HAs in the vegetable production areas, the herbicide oxyfluorfen showed a close relationship with the more aliphatic oxygenated structures, namely, CAₗₖyₗ-O,N, CAₗₖyₗ-O and CAₗₖyₗ-di-O.
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