In this study, the tips of Myriophyllum aquaticum (M. aquaticum) plants were planted in open-top plastic bins and treated by simulated wastewater with various ammonium-N concentrations for three weeks. The contents of related carbohydrates and key enzyme activities of carbon metabolism were measured, and the mechanisms of carbon metabolism regulation of the ammonia tolerant plant M. aquaticum under different ammonium-N levels were investigated. The decrease in total nonstructural carbohydrates, soluble sugars, sucrose, fructose, reducing sugar and starch content of M. aquaticum were induced after treatment with ammonium-N during the entire stress process. This finding showed that M. aquaticum consumed a lot of carbohydrates to provide energy during the detoxification process of ammonia nitrogen. Moreover, ammonia-N treatment led to the increase in the activitives of invertase (INV) and sucrose synthase (SS), which contributed to breaking down more sucrose to provide substance and energy for plant cells. Meanwhile, the sucrose phosphate synthase (SPS) activity was also enhanced under stress of high concentrations of ammonium-N, especially on day 21. The result indicated that under high-concentration ammonium-N stress, SPS activity can be significantly stimulated by regulating carbon metabolism of M. aquaticum, thereby accumulating sucrose in the plant body. Taken together, M. aquaticum can regulate the transformation of related carbohydrates in vivo by highly efficient expression of INV, SPS and SS, and effectively regulate the osmotic potential, thereby delaying the toxicity of ammonia nitrogen and improving the resistance to stress. It is very important to study carbon metabolism under ammonia stress to understand the ammonia nitrogen tolerance mechanism of M. aquaticum.

Rapid temperature rise in Arctic permafrost impacts not only the degradation of stored soil organic carbon (SOC) and climate feedback, but also the production and bioaccumulation of methylmercury (MeHg) toxin that can endanger humans, as well as wildlife in terrestrial and aquatic ecosystems. Currently little is known concerning the effects of rapid permafrost thaw on microbial methylation and how SOC degradation is coupled to MeHg biosynthesis. Here we describe the effects of warming on MeHg production in an Arctic soil during an 8-month anoxic incubation experiment. Net MeHg production increased >10 fold in both organic- and mineral-rich soil layers at warmer (8 °C) than colder (−2 °C) temperatures. The type and availability of labile SOC, such as reducing sugars and ethanol, were particularly important in fueling the rapid initial biosynthesis of MeHg. Freshly amended mercury was more readily methylated than preexisting mercury in the soil. Additionally, positive correlations between mercury methylation and methane and ferrous ion production indicate linkages between SOC degradation and MeHg production. These results show that climate warming and permafrost thaw could potentially enhance MeHg production by an order of magnitude, impacting Arctic terrestrial and aquatic ecosystems by increased exposure to mercury through bioaccumulation and biomagnification in the food web.

Bioconversion of recalcitrant keratinous biomass is one of the greatest ways to utilize products of feather hydrolysis and recycle them into bionetwork. Present study revealed 87% degradation of poultry feathers within 48 h in a constructed bioreactor using Chryseobacterium sp. RBT. The resulting feather hydrolysate (FH) was rich in soluble protein (3.56 ± 0.18 mg/ml), amino acids (3.83 ± 0.20 mg/ml), and macro and micro nutrients like N (8.0302%), P (0.3876%), K (0.5532%), Cu (0.0684%), Mg (0.8078%), Mn (0.2001%), Ca (0.4832%), Zn (0.0442%), and Fe (0.0330%). HPTLC analysis of FH revealed presence of tryptophan, cysteine, methionine, phenylalanine, glycine, valine, tyrosine, lysine, leucine, and serine as the primary amino acids. Field studies were conducted to apply FH as the bioenhancer to commercially important crops like brinjal and chilli through root drenching (20%, v/v). FH showed positive impact on the growth and development of plants along with early flowering and improved crop yield. In addition, nutritional quality of brinjal and chilli in terms of protein, amino acids, reducing sugars, phenolics, flavonoids, and antioxidant was elevated. Therefore, promotion and utility of by-products generated in feather degradation would be an effective strategy focusing on sustainable agricultural practices and problems associated with the waste management.

Plants are rich in biologically active compounds. They can be explored for the production of bioherbicides. In this context, the present work aimed to evaluate the allelopathic effect of hydroalcoholic extracts from two Solanaceae species: Solanum muricatum Ait. and Solanum betaceum Cav. For this end, we conducted phytochemical screening and biological assays, determining the effects of the extracts on germination, early development, cell cycle, and DNA fragmentation in plantlets and meristematic cells of the plant model Lactuca sativa L. (lettuce). The percentage of seeds germinated under effect of S. muricatum extract did not differ from the control, but plantlet growth was reduced at the highest concentrations. For S. betaceum extract, dose dependence was observed for both germination and plantlet development, with the highest concentrations inhibiting germination. The growth curves revealed the concentrations of 2.06 and 1.93 g/L for S. muricatum and S. betaceum extracts, respectively, as those reducing 50% of root growth (RG). At these concentrations, both extracts presented mitodepressive effect, besides inducing significant increase in the frequency of condensed nuclei, associated to DNA fragmentation and cytoplasmic shrinkage. The frequency of chromosome alterations was not significant. We further discuss the mechanisms of action related to the chemical composition of the extracts, which presented organic acids, reducing sugars, proteins, amino acids, and tannins, besides catechins and flavonoids, only found in the extract of S. betaceum.

Pretreatment is a vital step to enhance the yield of total reducing sugars and biofuel production from lignocellulose biomass. An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp. BMP01 strains, were isolated and identified from wood-feeding termite’s guts. Wheat straw was biodelignified by Ochrobactrum oryzae BMP03 bacteria strains to degrade lignin and to release the trapped cellulose and hemicellulose. The biodelignified wheat straw was hydrolyzed by Bacillus sp. BMP01 strains. Ochrobactrum oryzae BMP03-Bacillus sp. BMP01 consortia were also performed to analyze the effect of the simultaneous system. It was shown that the production of total reducing sugars in a separate hydrolysis system by Bacillus sp. BMP01 strain achieved 439 mg/g at 16 days of hydrolysis time, which is 9.45% higher than the simultaneous system. About 44.47% lignin was degraded by the Ochrobactrum oryzae BMP03 strain after 16 days of biotreatment. This also contributed for increment in cellulose content by 22.38% and hemicellulose content by 18.64%. The simultaneous system converted 368 mg of reducing sugars/g of wheat straw. Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time. Scanning electron microscope, mid-infrared analysis by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis confirmed the change in composition due to biotreatment. The biotreatment improved hydrolysis efficiency, which reduces the cost of biofuel production and increases the yield of biofuel. These results indicate the possibilities of biofuel production from wheat straw by employing Ochrobactrum oryzae BMP03 and Bacillus sp. BMP01 bacteria strains.

Honey is a natural substance produced by honey bees (the genus Apis) enjoyed by people due to its unique nutritional and medicinal properties. The aim of this study was to determine the physicochemical parameters (moisture, ash, water-insoluble content, reducing sugars, sucrose, free acidity, diastase activity, hydroxymethylfurfural, and electrical conductivity) and microbiological status (total number of aerobic mesophilic bacteria, total number of sulfite-reducing clostridia, the presence of Salmonella spp., total numbers of fungi and yeasts and the presence of Clostridium botulinum) in honey (honeydew, blossom, sunflower, acacia, and linden) produced in an urban environment in Serbia. We analyzed 19 apiary samples of honey, collected during the 2011 harvesting season, by using recommendation methods. Physicochemical parameters of the examined honey produced in the urban environment indicated the honeys were of acceptable quality. Bacillus spp. were detected in four honeys, yeasts were detected in three honeys, and Clostridium botulinum type E was detected in one honey using PCR. The current study also showed the presence of diverse honey varieties in Serbia.

In the course of searching for hepatoprotective agents from natural sources, the protective effect of chemical constituents of the marine red alga Alsidium corallinum (A. corallinum) against potassium bromate (KBrO₃)-induced liver damage in adult mice was investigated. The in vitro antioxidant and antibacterial properties of A. corallinum were firstly investigated. Then, A. corallinum was tested in vivo for its potential protective effects against damage caused by KBrO₃ in mice models divided into four groups: controls, KBrO₃, KBrO₃ + A. corallinum, and A. corallinum. Our results demonstrated the rich composition of A. corallinum in antioxidant compounds like phenolics, flavonoids, anthocyanins, polysaccharides, chlorophyll and carotenoids. Its antioxidant activity was also confirmed using β-carotene bleaching by linoleic acid assay, reducing sugar test and trolox equivalent antioxidant capacity. The ethanolic extract of A. corallinum also showed good inhibition of the tested bacteria. The coadministration of the red alga associated to the KBrO₃ alleviated hepatotoxicity as monitored by the improvement of hepatic oxidative stress biomarkers and plasma biochemical parameters, when compared to the KBrO₃-treated mice. These results were confirmed by the improvement of histological and molecular changes. Treatment with A. corallinum prevented liver damage induced by KBrO₃, thus protecting the body against free radicals and reducing inflammation and hypercholesterolemia risks.

The aim of the present study was to evaluate the phytotoxicity of olive mill wastewater (OMW) after being treated by the white-rot fungus Coriolopsis gallica. For this, the effect of irrigation with treated OMW (TOMW) and untreated OMW (UOMW) on tomato plants (Lycopersicon esculentum) for 3 weeks was studied. The control plants were irrigated with distilled water. Agronomic tests were performed in pot experiments in a greenhouse using the randomized complete block (RCB) experimental design. The relative leaf height (RLH), as a morphological parameter, and the content of total phenols in the roots and total chlorophyll [Cha + Chb] and reducing sugars in the leaves, as physiological parameters, were selected as responses of the experimental design. The results obtained showed that [Cha + Chb] in the leaves of tomato growth under TOMW was enhanced by 36.3 and 19.4 % compared to the plant growth under UOMW and to the controls, respectively. Also, reducing sugar concentrations were closed to those of the control plants, ranging from 0.424 to 0.678 g/L for the different dilutions tested. However, the plants irrigated with UOMW showed lower reducing sugar concentrations ranging from 0.042 to 0.297g/L. The optimum RLH (0.537) was observed in the plants irrigated with TOMW diluted at (1:4), this value being higher than that observed in the controls (0.438). Our study proved that the irrigation with TOMW significantly improved tomato growth and photosynthesis activity over those irrigated with UOMW. Optimization of TOMW as a fertilizer was obtained for a dilution of 1:4. From the obtained results, it can be concluded that OMW treated by C. gallica holds potential to be used as a fertilizer for tomato plants.

The enhanced H₂ production from maize straw had been achieved through the two-stage process of integrating H₂ fermentation and microbial electrolysis cells (MECs) in the present work. Several key parameters affecting hydrolysis of maize straw through subcritical H₂O were optimized by orthogonal design for saccharification of maize straw followed by H₂ production through H₂ fermentation. The maximum reducing sugar (RS) content of maize straw reached 469.7 mg/g-TS under the optimal hydrolysis condition with subcritical H₂O combining with dilute HCl of 0.3 % at 230 °C. The maximum H₂ yield, H₂ production rate, and H₂ content was 115.1 mL/g-TVS, 2.6 mL/g-TVS/h, and 48.9 % by H₂ fermentation, respectively. In addition, the effluent from H₂ fermentation was used as feedstock of MECs for additional H₂ production. The maximum H₂ yield of 1060 mL/g-COD appeared at an applied voltage of 0.8 V, and total COD removal reached about 35 %. The overall H₂ yield from maize straw reached 318.5 mL/g-TVS through two-stage processes. The structural characterization of maize straw was also carefully investigated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) spectra.

To characterize coprophilous fungi for converting lignocellulose into lipids, four fungal strains utilizing cellulose microcrystalline and xylan were screened. The fungi were identified as Cladosporium sp. F1, Circinella sp. F6, Mycocladus sp. F49, and Byssochlamys sp. F52 based on the ITS1-5.8S-ITS2 sequence similarity. The strain F52 accumulated 336.0 mg/L reducing sugars on cottonseed shells treated with ethanol. The combination of F1 + F52 increased the reducing sugar accumulating rates. However, the activities of avicelase and xylanase were not correlated with the reducing sugars accumulated by the test strains. Strains F6 and F52 produced higher cellular lipids (above 530.7 mg/L) than other strains. However, the strain F52 could produce more cellular lipids with xylose and mannose as the sole carbon sources. The results indicated that the reducing sugar contents accumulated by the different strains were influenced by the fungal taxa and ligocellulosic types. With fibrolytic and lipid accumulating activities, diverse fungi harboring in herbivore feces need to be further characterized.