Potato (Solanum tuberosum L.) production in irrigated coarse-textured soils requires intensive nitrogen (N) fertilization which may increase reactive N losses. Biological soil additives including N-fixing microbes (NFM) have been promoted as a means to increase crop N use efficiency, though few field studies have evaluated their effects, and none have examined the combined use of NFM with microbial inhibitors. A 2-year study (2018–19) in an irrigated loamy sand quantified the effects of the urease inhibitor NBPT, the nitrification inhibitor DMPSA, NFM, and the additive combinations DMPSA + NBPT and DMPSA + NFM on potato performance and growing season nitrous oxide (N₂O) emissions and nitrate (NO₃⁻) leaching. All treatments, except a zero-N control, received diammonium phosphate at 45 kg N ha⁻¹ and split applied urea at 280 kg N ha⁻¹. Compared with urea alone, DMPSA + NBPT reduced NO₃⁻ leaching and N₂O emissions by 25% and 62%, respectively, and increased crop N uptake by 19% in one year, although none of the additive treatments increased tuber yields. The DMPSA and DMPSA + NBPT treatments had greater soil ammonium concentration, and all DMPSA-containing treatments consistently reduced N₂O emissions, compared to urea-only. Use of NBPT by itself reduced NO₃⁻ leaching by 21% across growing seasons and N₂O emissions by 37% in 2018 relative to urea-only. In contrast to the inhibitors, NFM by itself increased N₂O by 23% in 2019; however, co-applying DMPSA with NFM reduced N₂O emissions by ≥ 50% compared to urea alone. These results demonstrate that DMPSA can mitigate N₂O emissions in potato production systems and that DMPSA + NBPT can reduce both N₂O and NO₃⁻ losses and increase the N supply for crop uptake. This is the first study to show that combining a nitrification inhibitor with NFM can result in decreased N₂O emissions in contrast to unintended increases in N₂O emissions that can occur when NFM is applied by itself.

High ammonia (NH₃) emissions from fertilized soil in China have led to various concerns regarding environmental safety and public health. In response to China's blue skies protection campaign, effective NH₃ reduction measures need to consider both mitigation efficiency and food security. In this context, we conducted a meta-analysis (including 2980 observations from 447 studies) to select effective measures based on absolute (AV) and yield-scaled (YSAV) NH₃ volatilization reduction potential, with the aim of establishing a comprehensive NH₃ mitigation framework covering various crop production sectors, and offering a range of potential solutions. The results showed that manipulating crop density, using an intermittent irrigation regime for paddy field rice, applying N as split applications or partially substituting inorganic fertilizer N with organic N sources could achieve reductions in AV and YSAV reduction of 10–20 %; adopting drip irrigation regimes, adding water surface barrier films to paddy fields, or using double inhibitor (urease and nitrification), slow-release or biofertilizers could achieve 20–40 % mitigation; plastic film mulching, applying fertilizer by irrigation or using controlled-release fertilizers could yield 40–60 % reduction; use of a urease inhibitor, fully substituting fertilizer N with organic N, or applying fertilizer by deep placement could decrease AV and YSAV by over 60 %. In addition, use of soil amendments, applying suitable inorganic N sources, or adopting crop rotation, intercropping or a rice-fish production model all had significant benefits to control AV. The adoption of any particular strategy should consider local accessibility and affordability, direct intervention by local/government authorities and demonstration to encourage the uptake of technologies and practices, particularly in NH₃ pollution hotspot areas. Together, this could ensure food security and environmental sustainability.

Ammonia (NH3) emission from agricultural sources has contributed significantly to air pollution, soil acidification, water eutrophication, biodiversity loss, and declining human health. Although there are numerous strategies for reducing NH3 emission from agricultural systems, the effectiveness of these measures is highly variable. Furthermore, the integrated assessment of measures to reduce NH3 emission both from livestock production and cropping systems based on animal and crop type is lacking. Therefore, we conducted a global meta-analysis and integrated assessment of measures to reduce NH3 emission from agricultural systems. Most of the studied mitigation strategies were effective in reducing NH3 emission. In the livestock production system, dietary additive, urease inhibitor (UI), manure acidification and deep manure placement have the highest mitigation potential relative to other mitigation strategies, with reduction ranges of 35.1–54.2%, 24.3–68.7%, 88.8–95.0%, and 93.8–99.7%, respectively, relative to the control, while manure storage management could significantly reduce NH3 emission by 70.0–82.1%. In the cropping system, fertilizer source, use of enhanced efficiency fertilizers, and method of field application are most effective for reducingNH3 emission. The use of ammonium nitrate, controlled release fertilizer (CRF), and deep placement of fertilizers could reduce NH3 emission by 88.3, 56.8, and 48.0%, respectively. Choosing a proper fertilizer is critical for decreasing NH3 emission from cropping systems. We conclude that carefully planned and adopted strategies suited for local conditions are promising for minimizing NH3 emission from agricultural systems on a global scale, while possible effects of those mitigation measures on the emission of greenhouse gases should be studied in the future.

Worldwide, nitrogen (N) deficiency is the main yield limiting factor owing to its losses via leaching and volatilization. Urease inhibitors slow down urea hydrolysis in soil by inhibiting urease enzyme activities whereas gibberellic acid is growth regulator. That is why, we evaluated the role of urease inhibitor [N-(n-butyl)thiophosphorictriamide (NBPT)] and gibberellic acid (GA₃) in improving nitrogen uptake and yield of maize under different N levels (120 and 150 kg ha⁻¹) along with control. Both N levels alone and in combination with GA₃ and NBPT significantly increased yield and yield components of maize over control. In addition, 150 kg N ha⁻¹ + NBPT + GA₃ produced highest biological, grain, and stover yields, 1000 grain weight, plant height, and N uptake exhibiting 33.15%, 56.46%, 27.56%, 19.56%, 23.24%, and 78% increase over 150 kg N ha⁻¹, respectively. The sole use of gibberellic acid or NBPT with each level of N also improved the yield and yield components of maize compared to sole N application and control. Furthermore, application of 120 kg N ha⁻¹ along with NBPT and GA₃ performed at par to 150 kg N ha⁻¹ + NBPT + GA₃ but it was superior than sole applied 150 kg N ha⁻¹ for all the studied traits. These results imply that application of GA₃ and/or NBPT can reduce dependence on urea and improve the yield and N uptake in maize by slowing urea hydrolysis in calcareous soils and shall be practiced.

Urea is considered as the most widely used nitrogen (N) fertilizer. Unfortunately, its application is associated with losses such as emissions of ammonia (NH₃) and nitrous oxide (N₂O) in a gas form. In addition to the economic loss, such N losses may threaten atmospheric quality. Application of both urease and nitrification inhibitors is advocated as an approach to mitigate these gaseous losses. Thus, laboratory studies were carried out to evaluate the effects of urease inhibitor-coated urea, nitrification inhibitor-coated urea, and other modified urea fertilizers on NH₃ volatilization and N₂O gas emissions in selected anaerobic rice soils. Copper (Cu) and Zinc (Zn) were selected as urease inhibitors and DMPP (3,4-dimethylpyrazole phosphate) as nitrification inhibitor. Nitrogen fertilizer treatments used were urea, Cu-coated urea (CuU), Zn-coated urea (ZnU), Cu + Zn-coated urea (CuZn), DMPP-coated urea (DMPPU), DMPP + Cu + Zn-coated urea (DMPPCuZn), OneBaja, sulfur-coated urea (SU), and dolomite-coated urea (DU). Results demonstrated that CuU, ZnU, DMPPCuZn, SU, and OneBaja were effective in reducing NH₃ volatilization by 12.12–37.48 % compared to urea, while DMPPU had no effect on NH₃ volatilization. Meanwhile, sulfur-coated urea (SU), CuU, ZnU, CuZn, OneBaja, DMPPU, and DMPPCuZn reduced N₂O emission over urea by 14.86, 17.57, 21.62, 29.73, 29.73, 33.78, and 48.64 %, respectively. These results suggest that using Cu, Zn, or combinations of DMPP, Cu, and Zn is recommended as an alternative to mitigate both NH₃ volatilization and N₂O emission, in addition to providing positive impact to environment.

Understanding the relationship between nitrogen (N) cycle and N transformation-related functional genes is crucial to reduce N loss during composting process. Urease inhibitor (UI) is widely used to reduce N loss in agriculture. However, the effects of UI on N transformation and related N functional genes during composting have not been well investigated. The goal of this study was to investigate the effects of a urease inhibitor (UI) on N functional genes and bacterial community succession during pig manure composting. Results showed that the addition of UI decreased the ammonium N content during the thermophilic stage and notably increased the total N and nitrite N contents of the final compost. The UI significantly decreased the abundances of amoA, nirS, nirK, and nosZ during the initial composting stage, while the opposite trend was observed at the maturation stage. Bacterial community richness and diversity were increased after the UI amendment, but the relative abundance of the phyla Firmicutes and Proteobacteria significantly decreased compared with control during the thermophilic stage. Redundancy analysis indicated that the evaluated environmental factors and bacterial community showed a cumulative 94.7% contribution to the total variation in N functional genes. In summary, UI addition is a recommended method for N conservation during composting, but the added forms of UI, such as delayed addition, combined with adsorbing materials, or microorganism inoculant, should be further evaluated.