International audience | Biofilm development in wastewater treatment system by soil infiltration is often mentioned for its participation to purification efficiency and clogging zone formation. It appears necessary to understand its evolution in order to better control the operation of these systems. The objective of this study was to improve knowledge about the temporal evolution of the biofilm structure in the first centimetres of infiltration system. For this purpose, metabolic fingerprints by Biolog EcoPlate (TM) and molecular fingerprints by Ribosomal Intergenic Spacer Analysis (RISA) were carried out on sand, septic effluent and treated effluent samples from two experimental reactors supplied with different hydraulic loads collected at different times. The metabolic capabilities of sand microflora decreased in time. In the same way, molecular structure of the biofilm community changed and converged to similar structure in time. Principal components analysis on RISA gel revealed a ''buffering effect'' of the sand filter on the genetic structure of the bacterial community from treated effluent. The kinetics of evolution of the both metabolic and genetic fingerprints showed a reduction of the metabolic and genetic potentials of septic and treated effluents for the same times. The population dynamic within the biofilms appears interesting evidence in the comprehension of the operation of the treatment systems.

International audience | Biofilm development in wastewater treatment system by soil infiltration is often mentioned for its participation to purification efficiency and clogging zone formation. It appears necessary to understand its evolution in order to better control the operation of these systems. The objective of this study was to improve knowledge about the temporal evolution of the biofilm structure in the first centimetres of infiltration system. For this purpose, metabolic fingerprints by Biolog EcoPlate (TM) and molecular fingerprints by Ribosomal Intergenic Spacer Analysis (RISA) were carried out on sand, septic effluent and treated effluent samples from two experimental reactors supplied with different hydraulic loads collected at different times. The metabolic capabilities of sand microflora decreased in time. In the same way, molecular structure of the biofilm community changed and converged to similar structure in time. Principal components analysis on RISA gel revealed a ''buffering effect'' of the sand filter on the genetic structure of the bacterial community from treated effluent. The kinetics of evolution of the both metabolic and genetic fingerprints showed a reduction of the metabolic and genetic potentials of septic and treated effluents for the same times. The population dynamic within the biofilms appears interesting evidence in the comprehension of the operation of the treatment systems.

A detailed analysis of different types of gravity distribution devices, designed to split on-site wastewater effluent equally between percolation trenches, has been carried out both in the laboratory and also in the field under realistic loading conditions. Four different types of distribution device have been compared: a V-notch distribution box, stilling chamber box and tee-splitters with and without baffles. The trials carried out in the laboratory with clean water showed that flow distribution for all devices was sensitive to both the off-level installation angles and variable flow rates, with the most stable performance achieved using the flow splitter with upstream baffle plates. In parallel to this, the on-site flow regime experienced at two sites was continuously monitored using a tipping bucket and data-logger over twelve month periods, finding that the most common flow rates at the distribution unit were in the range of 0.1–2.5 l min⁻¹. The on-site performance of these devices receiving both septic tank and secondary treated effluent showed that significant solid deposition and biofilm development had severely affected the equal distribution between the trenches, hence highlighting the need for regular maintenance to ensure efficient performance over time after installation.

The effect of aeration rate on nutrient removal from slaughterhouse wastewater was examined in two 10-L laboratory-scale sequencing batch reactors (SBRs--SBR1 and SBR2) operated at ambient temperature. The contaminants in the slaughterhouse wastewater had average concentrations of 4,000 mg chemical oxygen demand (COD) L⁻¹, 350 mg total nitrogen (TN) L⁻¹ and 26 mg total phosphorus (TP) L⁻¹. The duration of a complete SBR operation cycle was 8 h and comprised four operational phases: fill (7 min), react (393 min), settle (30 min) and draw/idle (50 min). During the react phase, the reactors were intermittently aerated four times at 50-min intervals, 50 min each time. DO, pH and oxidation-reduction potential (ORP) in the reactors were real-time monitored. Four aeration rates--0.2 L air min⁻¹ in SBR1 for 70 days, 0.4 L air min⁻¹ in SBR1 for 50 days, 0.8 L air min⁻¹ in SBR2 for 120 days and 1.2 L air min⁻¹ in SBR1 for 110 days--were tested. When the aeration rate was 0.2 L air min⁻¹, the SBR was continuously anaerobic. When the aeration rate was 0.4 L air min⁻¹, COD and TP removals were 90% but TN removal was only 34%. When the aeration rates were 0.8 and 1.2 L air min⁻¹, average effluent concentrations were 115 mg COD L⁻¹, 19 mg TN L⁻¹ and 0.7 mg TP L⁻¹, giving COD, TN and TP removals of 97%, 95% and 97%, respectively. It was found that partial nitrification followed by denitrification occurred in the intermittently aerated SBR systems.

A chemical and ecotoxicological assessment of treatment of wastewater that had been polluted with petroleum products using only Activated Sludge (AS) and four biologically activated sorbents (BASs), consisting of activated sludge plus: coal-based activated carbon (-C1), coconut shell-based activated carbon (-C2), zeolite (-Z), and anthracite (-A) were conducted. The efficiency and robustness of the four wastewater treatment systems were evaluated by calculating the reduced total petroleum hydrocarbon (TPH) and polycyclic aromatic hydrocarbon (PAH) contents and the acute ecotoxicity of the effluents. The chemical analysis showed that the combined treatment systems were very effective for reducing the total petroleum hydrocarbon and readily bioavailable PAH contents. The most efficient systems were the BAS-C1 and -C2, which removed 60-88% and 99.5-99.6% of TPH and PAH, respectively. The activated sludge-only treatment was the least effective for purifying the wastewater. Chemical oxygen demand was reduced by >90% by all carbon-based BASs (BAS-C1, BAS-C2 and BAS-A). Shifts in the relative composition of the individual PAHs were identified in samples taken before and after treatment. Algal and bacterial bioassays showed that the toxicities of effluents following treatment by all four systems (except AS for algae) were reduced by more than 80% and 90%, respectively. However, crustacean tests indicated that the carbon-based BASs reduced the toxicity [V tox₍₅₀₎] only by 19-67%. Our results indicated that the combination of sorption and biodegradation processes have great potential in the treatment of petroleum products polluted wastewater and is less sensitive for inhibitors of the biological process than treatments in which activated sludge alone is used. The assessment of chemical and ecotoxicological endpoints provided valuable information, but contrasting results for one of the assays indicates that further analysis on the capacity of the different treatment systems is warranted.

Biofilm development in wastewater treatment system by soil infiltration is often mentioned for its participation to purification efficiency and clogging zone formation. It appears necessary to understand its evolution in order to better control the operation of these systems. The objective of this study was to improve knowledge about the temporal evolution of the biofilm structure in the first centimetres of infiltration system. For this purpose, metabolic fingerprints by Biolog EcoPlate[trade mark sign] and molecular fingerprints by Ribosomal Intergenic Spacer Analysis (RISA) were carried out on sand, septic effluent and treated effluent samples from two experimental reactors supplied with different hydraulic loads collected at different times. The metabolic capabilities of sand microflora decreased in time. In the same way, molecular structure of the biofilm community changed and converged to similar structure in time. Principal components analysis on RISA gel revealed a “buffering effect” of the sand filter on the genetic structure of the bacterial community from treated effluent. The kinetics of evolution of the both metabolic and genetic fingerprints showed a reduction of the metabolic and genetic potentials of septic and treated effluents for the same times. The population dynamic within the biofilms appears interesting evidence in the comprehension of the operation of the treatment systems.

Under the present investigation phytoremediation of mercury and arsenic from a tropical open cast coalmine effluent was performed. Three aquatic macrophytes Eichhornia crassipes, Lemna minor and Spirodela polyrrhiza removed appreciable amount of mercury and arsenic during 21 days experiment. Removal capacities of these macrophytes were found in the order of E. crassipes > L. minor > S. polyrrhiza. Translocation factor (shot to root ratio of heavy metals) revealed low transportation of metals from root to leaves leading higher accumulation of metals in root as compared to leaves of the plant. It was evident from plant tissue analysis that mercury and arsenic up take by macrophytes had deteriorated the N, P, K, chlorophyll and protein content in these macrophytes. Correlations between removal of arsenic and mercury from mining effluent and its increase in plant parts were highly significant. Results favoured selected species to use as promising accumulator of metals.

The presence of pharmaceuticals and personal care products (PPCPs) and endocrine disrupting chemicals (EDCs) in treated wastewater is gaining attention due to their potential environmental impact. An analytical method was developed to quantify estrogen compounds in samples from a concentrated wastewater matrix typical of water recycling systems used in space. The method employed conventional HPLC with UV detection. Solid phase extraction (SPE) was used to isolate the compounds of interest from wastewater. Spike-recovery tests in clean and wastewater matrices were used to test the extraction process. The results of these experiments suggest that deconjugation is the most predominant reaction occurring in the systems, as effluent concentrations of free estrogens typically exceeded influent concentrations. Despite the long retention times of the system or the near infinite solids retention time, free estrogens were not removed from graywater representative of space waste streams. For a closed-loop wastewater treatment system, these compounds may accumulate to levels requiring other removal mechanisms (i.e., reverse osmosis).

This is a study of the sources of alkylphenol and alkylphenol ethoxylates in wastewater, including the new observation that the main contribution is from textiles. Alkylphenols and alkylphenol ethoxylates are widely used chemicals in various applications that are partly under environmental restrictions within Europe. The study sets out to analyze the most important sources of this large group of organic compounds in an urban wastewater system. A substance flow analysis (SFA) of the technosphere in Stockholm, Sweden in 2004 was conducted, allowing a comparison of 13 groups of goods' emissions to wastewater. It was found that the groups of textiles and cleaning agents were the major sources to wastewater, while the group's personal care products and paint and lacquers give smaller contributions. The content of alkylphenol ethoxylates in goods, especially in textiles, is a most significant source and is probably valid for other urban areas as well.

The pulp left after the extraction of juice from Citrus reticulate (kinnow), is a waste material, which was used as a potential sorbent for Cr(III) and Cr(VI) in the present study. The effect of experimental parameters such as pH, biosorbent dosage, biosorbent particle size, initial metal concentrations, temperature, shaking speed and sorption time on the Cr removal is apparent from the obtained results. The Freundlich isotherm and pseudo second order kinetic models fitted well to the data of Cr(III) and Cr(VI) biosorption by Citrus reticulata waste biomass. Effect of several pretreatments such as gases, natural coagulant and many other chemicals on Cr(III) and Cr(VI) sorption capacity of Citrus reticulata waste biomass was first time analyzed in the present study. The metal sorption capacity of Citrus reticulata waste biomass after a specific pretreatment was not only related to the nature of chemical but also strongly dependent on the oxidation state of the metal.