Different phytoplankton analysis methods (pulse-amplitude modulated fluorometry (PAM) and microscopy) were compared in preparation for compliance monitoring and enforcement with ballast water discharge standards. The key objective was to practically evaluate the performance of different new methods and tools to identify indicatively living phytoplankton cells of the size <50μm in minimum dimension and ≥10μm in minimum dimension as addressed by the Ballast Water Performance Standard (Regulation D-2, International Convention for the Control and Management of Ships' Ballast Water and Sediments, 2004). Four different PAM instruments were selected for the tests based upon knowledge and experience gained in different ballast water sample processing studies. The measurements of the PAM instruments were compared with epifluorescence microscope algae cell counts using fluorescein diacetate as viability stain. It was concluded that PAM fluorometry is a suitable method for indicative phytoplankton analysis of ballast water and the most accurate PAM instruments were identified.

Levels of Al, Sc, V, Co, Ni, As, Br, Rb, Sr, Ag, Sb, I, Cs, Ba, Th and U that were rarely or never studied, as well as the concentrations of classically investigated Mn, Fe and Zn in brown algae Cystoseira barbata C. Ag. and Cystoseira crinita (Desf.) Bory from the coastal waters of marine protected areas (Crimea, Black Sea), were determined using neutron activation analysis. Spatial variation and compartmentalization were studied for all 19 trace elements (TE). Concentrations of most TE were higher in “branches” than in “stems”. Spatial variations of V, Co, Ni and Zn can be related to anthropogenic activities while Al, Sc, Fe, Rb, Cs, Th and U varied depending on chemical peculiarities of the coastal zone rocks. TE concentrations in C. crinita from marine protected areas near Tarkhankut peninsula and Cape Fiolent, identified as the most clean water areas, are submitted as the background concentrations.

Two fish species yellowfin seabream (Acanthopagrus latus) and tonguesole (Cynoglossus arel) were collected from two locations in Kuwait’s territorial waters in non-reproductive periods and used as bio-indicator organism for the assessment of metals in the marine environment. Species variation in fish was observed; seabream contained high metal content and metallothionein in liver and gill tissues compared to tonguesole, especially from Kuwait Bay area. Oxidative injury was registered in the gills of both species, but in tonguesole liver was also involved. Consequently, antioxidant enzyme catalase was elevated in tonguesole enabling bottom dwelling fish to combat oxidative assault. The study provided information about the current status of metals in marine sediment and levels of metals accumulated in representative species along with oxidative damage in exposed tissues and the range of biomarker protein metallothionein and enzymes of antioxidant defence mechanism enhancing our understanding about the biological response to the existing marine environment in Kuwait.

Measurements of isoprene emissions, solar spectral radiation, temperature and relative humidity were carried out at a grassland site in the Inner Mongolia, China during the growing seasons in 2002 and 2003. Isoprene emissions are dependent on PAR (Photosynthetically Active Radiation) and temperature nonlinearly. PAR controls the main processes related to isoprene emission, thus, PAR energy balance is used to establish quantitative relationship between isoprene emission and its affecting factors. An empirical Equation of isoprene emission was built on the basis of PAR energy balance. The calculated values were in good agreement with those measured for 2002 and 2003 summer seasons, the relative biases of 70% estimated emissions were within 50% compared to measured fluxes. The chamber changes the inside environment and emission fluxes, the emission differences were estimated by using the empirical Equation. The results show that isoprene emission flux around the noon decreases by 37% when the chamber is used, i.e., the biggest effect was resulted from PAR difference caused by the chamber. Isoprene emission measured by chamber should be corrected. The empirical model of isoprene emission showed that isoprene emission fluxes were close to zero, when PAR was low in early morning and in late evening. Total isoprene emissions emitted from the grassland in the Inner Mongolia were 1.10 and 1.00gC m−2 during the growing seasons of 2002 and 2003, respectively, which contributed to about 3.1–4.3% and 2.8−3.9% to grass respiration. The averaged isoprene emission normalized to a standard light (1 500μmolm−2 s−1) and temperature (30 °C) condition was 482.8μg m−2 h−1.

Three monosaccharide anhydrides levoglucosan, mannosan and galactosan were detected in winter snowfall samples of 2012 in urban Beijing. Concentrations of three isomers vary from 0.15 to 54.43ngmL−1, with an average value of 10.49ngmL−1. Levoglucosan is the most abundant component. Winter snowfalls are contaminated by biomass burning emissions seriously in urban Beijing. The main sources are softwood and crop residue burnings around Beijing from late autumn to early winter, while long-range transport of biomass burning emissions contribute more during the late winter. Concentrations of monosaccharide anhydrides in snowfall samples may be affected by both topography and the meteorological conditions around urban Beijing.

The Mediterranean Sea is one of the most polluted seas worldwide, especially with regard to plastics. The presence of this emerging man made contaminant in marine environments precludes large effects and interactions with species exposed to massive litter quantities. In this review, available data of floating and seafloor litter around Mediterranean sub-basins are reported. A review of scientific literature on the interaction of plastic with marine biota resulted in the identification of 134 species, several taxa and feeding strategies affected from 1986 to 2014. Data from 17,334 individuals showed different levels of ingestion and effects on catalogued IUCN species (marine mammals and sea turtles) in addition to several pelagic fish and elasmobranchs. Biodiversity is certainly under threat, and knowledge of the extent of taxa affected is of concern considering the increasing plastic loads in the Mediterranean Sea and worldwide.

The objective of this study was to evaluate the effect of crude oil on water transport through mangroves roots in the presence and absence of dispersants. Water transport through the roots were evaluated experimentally using red mangrove root segments exposed to salt water contaminated with Louisiana crude oil for seven days in the presence and absence of Corexit 9500A (dispersant). Experimental observations were interpreted in view of the structural integrity and fouling phenomena observed on the epidermis and endodermis layers of the roots. The effects of oil on the radial water flux through the epidermis and endodermis were analyzed using a dual layer filtration model. Progression of fouling due to accumulation and penetration of the contaminants through the root layers were interpreted in relation to observed mangrove health (long and short term effects) reported in the literature.

The indoor environment of schools where children stay for long periods of time is a matter of primary concern due to the vulnerability and sensibility of children to air pollutant exposure. The aims of this study were to characterize the indoor air quality of elementary schools in Seoul, Korea, to compare the indoor levels according to the surrounding environments and year of remodeling. The air pollutants, including particle matter (PM10), nitrogen dioxide (NO2), carbon monoxide (CO), carbon dioxide (CO2), total bacteria count (TBC), total volatile organic compounds (TVOC) and formaldehyde (HCHO), were sampled during autumn from classrooms and laboratories at 116 elementary schools employing natural ventilation. The schools were selected based on their surrounding environments: surrounded by buildings (SB), roadways (RW), and mountains (MT). The indoor air quality measured at elementary schools in Seoul, Korea showed generally low levels of pollutants due to low occupancy and adequate ventilation and the indoor levels of PM10 and TBC in schools near roadways were significantly lower than other surrounding environments due to dispersion of pollutants, without obstruction by buildings and mountains. TVOC and HCHO concentrations of schools remodeled within a year were significantly higher. Therefore, suitable management for school building characteristics is needed.

Ground–level ozone (O3) is known to exhibit strong daily variations that lead to complexity of the pollutants’ analysis and predictions. This study aimed to introduce and explore the variations in O3 concentrations during daytime (DT), nighttime (NT), and critical conversion time (CCT) using multiple linear regression (MLR) and principal component regression (PCR) analyses. The original variables and principal component analysis (PCA) results were used as the input for MLR analysis. Hourly averages of six air pollutants and four meteorological parameters at Shah Alam during 1999–2009 were selected for this study. The monitoring records in 2010 were used to assess the developed models using several performance indicators. Results showed that the MLR model during DT exhibited optimal performance in terms of normalized absolute error, index of agreement, prediction accuracy, and coefficient of determination (R2) with values of 0.2762, 0.9211, 0.8581, and 0.7354, respectively. PCR during CCT also showed significantly higher performance than that during DT and NT. This result was evidenced by higher percentage of total variances, which could be explained by the selected variables in PCA during CCT.

‘Collect once, use often’ is a frequently cited principle in both national and international efforts to promote the collection, archiving and sharing of marine monitoring data. Since the implementation of the Marine Conservation Zone (MCZ) evidence collection programme, 67 recommended MCZ sites have been visited and a suite of marine data collected. Here we present how this dataset was utilised outside of the MCZ programme to identify occurrences of non-indigenous species (NIS) around the UK coast. One hundred and thirty-five aquatic species from the Non-native Species Information Portal (NNSIP) register were used to produce a standard list of NIS against which, infauna and epifaunal data records from the MCZ project were compared. A total of 20 NIS were identified across 42 of the 67 sites surveyed. This study demonstrates that with sufficient coordination and management data collected for other purposes can be easily utilised to address additional policy requirements.