The accumulation of metals and nutrients in biosolid-amended soils and the risk of their excess uptake by plants is a topic of great concern. This study examines the elemental uptake and accumulation in five vegetable plants grown on biosolid-applied soils and the use of spectral reflectance to monitor the resulting plant stress. Soil, shoot, root, and fruit samples were collected and analyzed for several elemental concentrations. The chemical concentrations in soils and all the plant parts increased with increase in applied biosolid concentrations. The Cu and Zn concentrations in the plant shoots increased in the order of collard < radish < lettuce < tomato < pepper. The Cu and Zn concentrations accumulated significantly in the fruits of the tomato plants compared to other plants. Among all the plants, the shoot concentration factor (SCF) of Zn was significantly higher for pepper plants, indicating increase in uptake of Zn. The shoot relative uptake index (SRUI) of Cu and Zn increased in the order of collard < radish < lettuce < tomato < pepper. The shoot dry weight and spectral reflectance of the radish plants in the near-infrared (NIR) region (800–1,300 nm) decreased significantly with increase in biosolid concentration compared to other plants. Increase in plant stress with increase in biosolid dose was evident in radish plants through significant reduction in Normalized Difference Vegetative Index (NDVI). This study indicates the potential use of spectral reflectance as a tool for the screening and monitoring of stress-sensitive plant species and their physiology and as a result, indirectly assesses the chemical concentrations in soils and plants.

Investigations on the pollution of groundwater with pathogenic microorganisms, e.g. tracer studies for groundwater transport, are constrained by their potential health risk. Thus, microspheres are often used in groundwater transport studies as non-hazardous surrogates for pathogenic microorganisms. Even though pathogenic microorganisms occur at low concentrations in groundwater, current detection methods of microspheres (spectrofluorimetry, flow cytometry and epifluorescence microscopy) have rather high detection limits and are unable to detect rare events. Solid-phase cytometry (SPC) offers the unique capability of reliably quantifying extremely low concentrations of fluorescently labelled microorganisms or microspheres in natural waters, including groundwater. Until now, microspheres have been used in combination with SPC only for instrument calibration purposes and not for environmental applications. In this study, we explored the limits of the SPC methodology for its applicability to groundwater transport studies. The SPC approach proved to be a highly sensitive and reliable enumeration system for microorganism surrogates down to a minimum size of 0.5 μm, in up to 500 ml of groundwater, and 0.75 μm, in up to 1 ml of turbid surface water. Hence, SPC is proposed to be a useful method for enumerating microspheres for groundwater transport studies in the laboratory, as well as in the field when non-toxic, natural products are used.

Potassium silicate drilling fluid (PSDF) is a relatively new type of drilling waste generated by the oil and gas industry. PSDF effects on soil, vegetation, and ground water must be determined before its land disposal and use in reclamation can be regulated. A laboratory column leachate study was conducted to quantify the response of select soil and leachate properties to PSDF at various depths in soil column profiles. A spent PSDF was applied to two soils (sand and loam textures) at four rates (20, 40, 60, 120 m³ ha⁻¹) with two application methods (incorporated, sprayed). Changes to soil and leachate properties were at values that would not be detrimental to most plant species when PSDF was applied at ≤60 m³ ha⁻¹. Applying PSDF at 120 m³ ha⁻¹had significant effects on soil properties and leachate quality. Hydraulic conductivity and field capacity were significantly reduced, and soil available potassium and sulfate concentrations, pH, and salinity increased with PSDF. Incorporated PSDF in the upper 10 cm of soil accelerated PSDF element transport through soil columns to leachate and increased organic carbon and salinity in leachate. PSDF application rate significantly reduced soil field capacity, available nitrogen, and increased salinity at the highest rates in loam soil, suggesting a threshold beyond which conditions will not be suitable for land spraying PSDF. This research demonstrates that PSDF has potential to improve soil short term water availability, macronutrient potassium and sulfur for disposal on cultivated and uncultivated lands. This potential should be field tested.

An inexact left-hand-side chance-constrained programming (ILCCP) was proposed and applied to a nonpoint-source water quality management problem within an agricultural system. The ILCCP model can reflect uncertainties presented as interval parameters (manure mass balance, crop nutrient balances, energy and digestible protein requirements, pollutant losses, water quantity constraints, technical constraints, and so on) and left-hand-side random variables (nitrogen requirement of crop i) at the same time. A non-equivalent linearization form of ILCCP was deduced and proved intuitively, which can help handle the left-hand-side random parameters in the constraints. The decision schemes through ILCCP were analyzed under scenarios at different individual probabilities (p ᵢ , denotes the admissible probability of violating the constraint i). The performance of ILCCP was also compared with the corresponding interval linear programming model. A representative nonpoint-source water quality management case was employed to facilitate the analysis and the comparison. The optimization results indicated that the net system benefit in the water quality management case would decrease with increasing probability levels on the whole. This was because that the higher constraint satisfaction of probability would lead to stricter decision space. The optimal scheme shows an obvious downtrend in the application amount of manure as the violation probability levels decreasing from scenarios 1 to 3 (p ᵢ = 0.1, 0.05 and 0.01). This demonstrates that the application amount of manure would be reduced effectively by adjusting strictness of the constraints. This study is the first application of the ILCCP model to water quality management, which indicates that the ILCCP is applicable to other environmental problems under uncertainties.

Phytoremediation can be assisted by microorganisms, which promote plant growth and increase heavy metal availability in soil. In this study, we aimed at evaluating the effect of two plant growth-promoting bacteria (PGPB) on phytoextraction of copper (Cu) by maize. We chose the strains based on their ability to synthesize indole compounds, produce siderophores, solubilize phosphorus, and increase soil conductivity and extractable Cu in soil. Then, in glasshouse experiments, we assessed their ability to increase biomass, chlorophyll content, and Cu extraction by maize. Results showed that Acinetobacter sp. RG30 and Pseudomonas putida GN04 were overall the most active strains to synthesize indole, produce siderophores, and solubilize phosphorus, and hence selected for further studies. Also, both were able to significantly increase soil conductivity and release Cu from soil compared to control. Glasshouse experiments showed that Cu had a negative effect on plant growth, but inoculation with bacteria promoted plant growth and chlorophyll content in its presence (p < 0.05). Notably, the effect of inoculation on plant growth was larger on contaminated than on uncontaminated soil, which suggests an overall bacterial effect for alleviation of stress caused by Cu. Inoculation with RG30 or GN04 improved Cu extraction by maize (p < 0.05); interestingly, co-inoculation led to the highest accumulation (200 μg Cu/g plant dry weight). We conclude, therefore, that inoculation with RG30 and GN04 improves metal extraction by increasing plant growth, fitness, and availability of minerals in soil, which represents an important tool for the improvement of phytoextraction processes in polluted environments.

Pyrene, a four-ring polycyclic aromatic hydrocarbon that is highly resistant to degradation, persists in the environment and exerts its harmful effects toward humans, flora, and fauna when accumulated to a certain level. The ineffectiveness of conventional physical–chemical treatment methods has urged the emergence of biological treatments to degrade pyrene that persists in the environment. In this study, Pleurotus eryngii F032 was originally isolated from our laboratory due to its ability to degrade pyrene. Optimum conditions for pyrene degradation were determined using five different parameters, including pyrene concentration, incubation temperature, pH, agitation, and rhamnolipid concentration. The culture was incubated for 7, 15, 23, and 30 days, respectively, followed by pyrene extraction for degradation analysis. Results show that lower pyrene concentration requires less time for degradation by P. eryngi F032. Moreover, more time is needed for degradation when higher concentration is used, resulting in slower degradation. Optimum pyrene degradation conditions by P. eryngii F032 have been recorded at 40 °C incubation temperature, pH 3, and 2.5 % of rhamnolipid concentration with an agitation speed of 120 rpm. The capability of P. eryngii F032 to utilize pyrene as carbon and energy source depends on the presence of ligninolytic enzymes. The formation of protocatechuic acid resulting from pyrene degradation was detected via GC-MS analysis, which was further confirmed through spectrophotometric analysis.

Cytostatic drugs are pharmaceutically active compounds used in chemotherapy to prevent or disrupt cell division. Only a few environmental studies have been focused on cytostatic drugs, in spite of their toxicity, their increasing consumption, and their discharge into municipal sewage. This fact can be mainly due to the lack of methods for their simultaneous analysis. This research describes the occurrence of 14 cytostatic drugs in influent and effluent wastewater from four wastewater treatment plants located in Seville (Spain) during 1-year period. A preliminary environmental risk assessment was also carried out. Five cytostatic drugs (cytarabine, etoposide, gemcitabine, iphosphamide, and methotrexate) were detected in influent wastewater at concentration levels up to 464 ng L⁻¹(cytarabine). Six of them (cytarabine, doxorubicin, gemcitabine, iphosphamide, paclitaxel, and vinorelbine) were detected in effluent wastewater at concentration levels up to 190 ng L⁻¹(cytarabine). Most of the detected cytostatic drugs are not significantly removed during wastewater treatment. Nevertheless, neither ecotoxicological nor genotoxical risks are expected to occur at the measured concentrations on the aquatic environment.

This contribution is concerned with the comparison of the efficiency of the removal of four pure neonicotinoid active ingredients (AIs) and their commercial formulations (CFs) from aqueous solutions by using different advanced oxidation processes at the pH 2.8. The AIs of thiamethoxam and imidacloprid, and their CFs (Actara and Confidor), having a nitroguanidine functional group, exhibited low persistence to photolysis. In contrast to them, thiacloprid and acetamiprid and their CFs (Calypso and Mospilan), containing a cyanoimine functional group, were stable during the UV irradiation period. As expected, the degradation rate of the studied neonicotinoids increased significantly in the combined action of UV radiation and H₂O₂. In the case of thiacloprid and acetamiprid and their CFs, the reaction of the OH radicals formed and molecules of these insecticides was the major destruction pathway. The increased photodegradation efficiency of the UV/7.2Fe/TiO₂/H₂O₂ and vis/7.2Fe/TiO₂/H₂O₂ processes was attributed to the surface photoreduction of Fe³⁺ to Fe²⁺, which produces new OH radicals in the reaction with H₂O₂. In the presence of visible light, the efficiency may be partly due to the formation of the H₂O₂–TiO₂ complexes. For the 7.2Fe/TiO₂/H₂O₂ process in the presence of UV or visible radiation, no significant influence on the efficiency of photodegradation was observed in dependence of the structural differences of selected neonicotinoids. These results strongly suggest that highly reactive hydroxyl radicals, generated on the catalyst’s surface in the reaction involving H₂O₂, are responsible for this oxidation. In order to investigate degree of mineralization for all insecticides, TOC measurements were also conducted. Also, it was observed that the removal of pure AIs and their CFs by dark adsorption was almost negligible.

The main objective of this study was to evaluate residuals from 28 pharmaceuticals and three phthalate esters (PAEs) in drinking waters, which were stored and further purified in different manners. Samples of drinking water from two different supply networks in Taiwan were collected in two batches from two research institutes (i.e., sampling sites N and S) in this study. Each batch of sampling was conducted on one Friday afternoon and the next Monday morning. Water storage tanks used in these two sampling sites are composed of different materials. Sampling points at each sampling site included one tap water pipeline, five water storage tanks, and five drinking fountains. It was found that retention of drinking water in the storage tanks over the weekend would be beneficial to spontaneous degradation of pharmaceuticals and PAEs. The preliminary results also showed that city water might have dissolved DiNP from modular water tanks made of fiberglass-reinforced plastics, whereas no such evidence was observed for water tanks made of stainless steel. Furthermore, a trace amount of pharmaceuticals and PAEs still could be detected in city waters, even in drinking fountain water.

The application of combined electrochemical and photochemical techniques for the degradation of real textile effluent is presented. It is demonstrated that the simultaneous use of both techniques, in conjunction with in situ generation of free chlorine and its subsequent photolysis, is a promising technique for removing color and chemical oxygen demand (COD) from effluents. Crucially, the combination of electrochemical and photochemical techniques leads to lower quantities of chlorine-containing degradation by-products being produced and no overall increase in toxicity. Over the treatment times studied, up to 65 % less chloride-containing degradation by-products are formed while at the same time greater rates of color and COD removal are achieved.