We determined normal plasma butyrylcholinesterase (BChE), carboxylesterase (CbE using α-NA substrate), and glutathione S-transferase (GST) activities in Caiman latirostris and Phrynops hilarii to obtain reference values for organophosphorus (OP) pesticide monitoring. BChE and CbE sensitivity to malaoxon was also evaluated. C. latirostris (N = 12; six males and six females) and P. hilarii (N = 12; seven males and five females) were obtained from the programs Yacaré (Entre Ríos Province, Argentina) and Zoo of Córdoba (Córdoba Province, Argentina). Mean total (female and male) plasma BChE activity was significantly different between reptile species, ranging between 0.337 ± 0.085 μmol min−1 ml−1 of plasma for C. latirostris and 0.251 ± 0.070 μmol min−1 ml−1 of plasma for P. hilarii. However, plasma CbE (α-NA) and GST activities were significantly higher in P. hilarii (4.81 ± 1.00 and 0.145 ± 0.045 μmol min−1 ml−1 of plasma, respectively) than in C. latirostris (0.57 ± 0.20 and 0.059 ± 0.013 μmol min−1 ml−1 of plasma, respectively). No significant differences in B-esterase and GST activities were detected between sexes, except CbE (α-NA) for C. latirostris. IC50 values for BChE and CbE (α-NA) suggested different sensitivity levels between species and between sexes. The results demonstrate that plasma esterase activity varied between species, but not between sexes (except CbE for C. latirostris). The in vitro inhibition tests indicated that CbE (α-NA) is more sensitive to inhibition than BChE. C. latirostris may be the reptile species most vulnerable to field pesticide exposure because this reptile presents the lowest CbE activity levels and its B-esterase levels seem more sensitive to OP.

This study investigated the encapsulation and photocatalysis of chlorophenol compounds in water using porphyrin-(polystyrene-b-2-dimethylaminoethyl acrylate) star polymer. The chloride ions generated during photocatalytic process were identified and quantified. 2,4,6-Trichlorophenol, pentachlorophenol, and 2,4-dichlorophenol were satisfactorily decomposed in the photoreactor using porphyrin-(polystyrene-b-2-dimethylaminoethyl acrylate) star polymer, with removal efficiencies of 2,454, 498, and 760 mg/g of porphyrin-(polystyrene-b-2-dimethylaminoethyl acrylate) star polymer. The half-life times reached around 30 min, with the exception of that for 2,4-dichlorophenol. The star polymer-impregnated porphyrin is a promising photocatalyst for the removal of chlorophenols.

Tests were conducted to study the influence of non-ionic surfactants Triton X-100 and Tween 80 on the removal of mixed contaminants from a sandy soil using phytoremediation. Cd(II) and Pb(II) were used to form the inorganic contaminant, while used engine oil was selected to form the organic contaminant. The Indian mustard (Brassica juncea) plant was the plant chosen for phytoremediation of the sandy soil that contained the mixed contaminant. Thirty days after the plants were grown in the greenhouse, surfactants were applied to test pots in which the soil had been spiked with 50 mg kg−1 of CdCl2, 500 mg kg−1 of PbCl2 and 500 mg kg−1 of used engine oil. Two control tests were conducted in this study. Planted and unplanted control tests were conducted using soil without surfactants. Following these tests, the tests were completed using the plants and surfactants at different concentrations. Test results showed that Triton X-100 and Tween 80 at concentrations higher than their critical micellar concentration enhanced Cd(II) and Pb(II) accumulation in the plant roots. Further, test data showed that translocation of contaminants to plant shoots occurred for Cd(II) but not for Pb(II). At the same concentrations, Tween 80 was more effective than Triton X-100 in facilitating rhizodegradation of used engine oil. This study demonstrates that simultaneous phytoremediation of Pb(II), Cd(II) and oil can be enhanced by using non-ionic surfactant Tween 80. Leaching test results indicated that the enhanced phytoremediation could remove the mixed contaminants safely from the point of view of limiting groundwater contamination.

This paper reviews the ecological environment protection measures of Chinese rural hydropower development schemes. China’s rapid economic growth is making great energy demands and developing rural hydropower currently provides 30.4 GW annually. There is a conflict of interest between hydropower development and ecological and environmental protection. Potential problems include changes in hydrological condition, eutrophication, downstream nutrient reduction, sediment deposition, aquatic ecosystem alteration, and ecological water demand variation. Since most of the rural hydropower resources are located in ecologically fragile regions, it is significantly more important that ecological environment protection is being considered. As exploitation of rural hydropower has developed in China, ecological environment protection schemes had to adapt to changing boundary conditions. Due to improvements in environmental protection management, various environmental impact assessment methods have been applied including fuzzy analysis hierarchy process, ecological scheduling, pressure-state-response, and ecological environment evaluation index. The latter index can both qualitatively and quantitatively analyze ecological environment impacts and has become the most frequently used tool in the evaluation of rural hydropower exploitation. To rebalance the interests regarding energy generation and environmental protection, countermeasures originating from different aspects such as engineering design optimization, management improvement, and ecological restoration were recommended to promote ecological environment protection. The review concludes that by taking ecological environment protection into consideration in the whole rural hydropower plan, adopting ecological scheduling to guarantee river ecological water demand and implementing ecological restoration in watershed management are the most effective approaches in furthering sustainable development of rural hydropower.

Using an automated gas chromatography system coupled with an online sampling/thermal desorption module, benzene, toluene, and their alkylated derivatives were measured in Las Vegas, Nevada from 3 July to 28 August 2008. The levels of hydrocarbons were comparable to those typically found in urban environments. Statistically significant (at 95 % level) higher concentrations were measured on mid-week days as compared with those measured during weekends. This was correlated to a local traffic pattern rather than traffic on highways. The concentrations of aromatic hydrocarbons also increased during periods when transport of smoke from wildfires in central and north California was identified by remote sensing but these levels were comparable to other days with volatile organic compounds concentrations. The high toluene/benzene ratios and the estimated photochemical age of air masses implied the contribution of other local sources. Fuel evaporation accounted for the vast majority of toluene enhanced concentrations in early July (as compared with those measured in June) for sites within the urban grid, but very little for sites located outside the urban area.

Over a century of metal processing activity has resulted in widespread metal contamination of soils in Sudbury, ON, Canada. To assess the potential for recovery from the large reductions in metal deposition, critical loads were estimated for metals at 415 sites in Sudbury using an ‘effects based’ approach that is based on exceedance of provincial soil guidelines using multiple independent estimates of metal partitioning (Kd) for each metal. Sudbury soils are heavily contaminated with copper (Cu) and nickel (Ni), with 74 % of samples currently exceeding the provincial soil guideline for Cu and 87 % of samples exceeding the guideline for Ni. Both metals are strongly correlated with other metals (zinc (Zn), cadmium (Cd), lead (Pb)), although they rarely exceed provincial guidelines Copper and Ni are also strongly correlated with organic matter but not soil pH. Based on the most recent Cu and Ni deposition estimates (mid-1990s), it is estimated that between 20 % and 51 % of the sites receive deposition in excess of the ‘effects based’ critical load for Cu and between 5 % and 97 % of sites receive atmospheric deposition in excess of the ‘effects based’ critical load for Ni. These results suggest that Cu and Ni concentrations in soil will generally decrease resulting in slightly fewer sites that exceed the provincial soil guideline, but that the timeframe of this response will be very slow, with relatively little change occurring over the next 100 years. Even assuming a best case deposition scenario whereby Cu and Ni deposition were to immediately fall to background levels, the percentage of sites with Cu and Ni levels in excess of the OMOE guideline would still be between 69 % and 72 %, and 56 % and 86 %, respectively, demonstrating that while recovery of the Sudbury soils is possible, greater reductions in metal deposition are needed and even so, it will be a process that takes several centuries.

Contact time, pH, fluoride concentration, and sorbent dose effects on the removal of fluoride ions by a carbonaceous material obtained from pyrolysis of sewage sludge (CM) were evaluated. Equilibrium was reached after 18 h of contact time and the maximum sorption was found at pHeq = 7.06 ± 0.08, which corresponds to the zero charge point of the CM. The highest efficiency in the sorption system for fluoride removal (2.84 ± 0.03 mg F− [Formula: see text]) was found with 0.4 gCM L−1 and with 20 gCM L−1, 82.2 ± 0.5% of fluoride was removed. The kinetic data of the process could be fitted to the pseudosecond order and the intraparticle mass transfer diffusion models, whereas isotherm to the Langmuir–Freundlich equation. These results indicate that the mechanism is chemisorption on a heterogeneous material. Fluoride ions were best partially desorbed using a bicarbonate ions solution and the material was partially regenerated by using a solution of HCl (pH = 1).

Low impact development (LID) is a land development strategy for managing stormwater at the source with decentralized micro-scale control measures. Since the emergence of LID practices, they have been successfully used to manage stormwater runoff, improve water quality, and protect the environment. However, discussions still surround the effectiveness of many of these practices, resulting in a reluctance to widely adopt them. This paper highlights evidence in the literature regarding the beneficial uses of LID practices. A discussion of how LID practices are represented in hydrologic/water quality models is also provided using illustrative examples of three computational models developed with algorithms and modules to support widespread adoption of LID practices. Finally, the paper suggests directions for future research opportunities.

Polybrominated diphenyl ethers (PBDEs) are highly persistent anthropogenic contaminants found in trace amounts in many environmental compartments far from their source areas, posing a risk to aquatic ecosystems. Our objective was to determine the relative toxicities of three BDEs, BDE-47, BDE-99 and BDE-154 on marine phytoplankton algae Isochrysis galbana. For a highly sensitive endpoint: the 72-h inhibition of autotrophic growth rate was calculated according to standards methods. Actual PBDE concentration was measured by GC-MS and toxicity parameters were calculated on the basis of time-weighted mean actual concentrations. No observable effect concentration (NOEC) values were 2.53 μg L⁻¹ for BDE-47, 3.48 μg L⁻¹ for BDE-99 and 12.3 μg L⁻¹ for BDE-154, and LOEC values were 5.06, 6.96 and 24.60 μg L⁻¹ for BDE-47, BDE-99 and BDE-154, respectively. The calculated IC₁₀ (the concentration inhibiting growth rate by 10 %) corresponded to 9.3, 12.78 and 54.6 μg L⁻¹ for BDE-47, BDE-99 and BDE-154, respectively. The 50 % inhibitions of growth rate (IC₅₀) values were: 25.7 μg L⁻¹ BDE-47, 30.0 μg L⁻¹ BDE-99 and 243.7 μg L⁻¹ BDE-154. Therefore, the acute toxicity of PBDEs decreases as the degree of bromination increases, the order of toxicity is BDE-47 > BDE-99 > BDE-154. Significant (p < 0.05) adverse effects were observed for all compounds at concentrations >15 μg L⁻¹. Our results indicated that under laboratory conditions PBDEs inhibited the growth of marine phytoplankton at concentrations near 10 μg L⁻¹. However, further work is required to investigate long-term effects in these and other aquatic organisms.

In domestic wastewater, bacterial physiology controls cell production (growth, replication) and cell maintenance, determining how energy is allocated between these two processes. The aim here was to develop a method to quantify these cellular processes so that the bacterial physiological state could be manipulated to lower this source of pollution. We simultaneously used the incorporation of radiolabelled thymidine into DNA (a measure of new cell synthesis) and leucine into protein in wastewater to quantitatively distinguish bacterial growth from maintenance processes. We found that DNA and protein syntheses were coupled in wastewater after substrate enrichment (with glucose or acetate)—balanced growth. Once the substrate was depleted, the two processes became uncoupled—unbalanced growth. In this physiological state, the bacteria were synthesising protein, but fewer bacteria were replicating. More energy was allocated to cell maintenance than replication. A mean Leu/TdR ratio of 7.4 was determined for wastewater and was similar to natural aquatic ecosystems. As the bacterial growth rate decreased, the Leu/TdR ratios increased. We show how the simultaneous measurement of [³H]Leu and [³H]TdR quantitatively distinguishes balanced from unbalanced growth. Low [³H]Leu/[³H]TdR ratios indicated bacteria were physiologically stressed, an ideal state for biological wastewater treatment processes (WWTP) as the bacteria divert more energy to maintenance activities instead of growth. Leu/TdR ratios of 70 have been recorded in natural aquatic ecosystems which suggests WWTP have potential to be manipulated to achieve much higher Leu/TdR ratios than we report here. Changes to plant operation to improve operation efficiency include finding the optimum rate of substrate (pollution supply) or alternating aerobic and anaerobic periods to maximise the Leu/TdR ratio to achieve less biomass production for land disposal and more cost-effective operation that generates less pollution in the effluent.