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Résultats 1151-1160 de 4,896
Biotransformation and detoxification of the neonicotinoid insecticides nitenpyram and dinotefuran by Phanerochaete sordida YK-624
2019
Wang, Jianqiao | Tanaka, Yusuke | Ohno, Haruka | Jia, Jianbo | Mori, Toshio | Xiao, Tangfu | Yan, Bing | Kawagishi, Hirokazu | Hirai, Hirofumi
Neonicotinoid insecticides have been widely used throughout the world over the last two decades. In the present study, we investigated the degradation of neonicotinoid insecticides nitenpyram (NIT) and dinotefuran (DIN) by the white-rot fungus Phanerochaete sordida YK-624. While NIT was completely degraded by P. sordida YK-624 under ligninolytic conditions, only a 20% decrease was observed under nonligninolytic conditions. On the other hand, P. sordida YK-624 degraded 31% of DIN under ligninolytic conditions after a 20-day incubation, while it did not degrade DIN under nonligninolytic conditions. We found that cytochromes P450 played a key role in the biotransformation of NIT and DIN by P. sordida YK-624. A novel NIT metabolite (E)-N-((6-chloropyridin-3-yl)methyl)-N-ethyl-N′-hydroxy acetimidamide (CPMHA) and a novel DIN metabolite N-((4aS,7aS,E)-1-methylhexahydrofuro[2,3-d]pyrimidin-2(1H)-ylidene)nitramide (PHPF) were identified in this study. In addition, to evaluate neurotoxicity, the effects of NIT, DIN and their metabolites on the viability of human neuroblastoma cells SH-SY5Y were determined. PHPF showed higher neurological toxicity than DIN, whereas the metabolite of NIT, CPMHA, showed no toxic effect. Our results indicated that the neurological toxicity of NIT could be effectively removed by P. sordida YK-624.
Afficher plus [+] Moins [-]Effects of titanium dioxide nanoparticles on algal and bacterial communities in periphytic biofilms
2019
Hou, Jun | Li, Tengfei | Miao, Lingzhan | You, Gouxiang | Xu, Yi | Liu, Songqi
The widespread application of commercial TiO₂ NPs inevitably leads to their release into environmental waters through various ways. TiO₂ NPs released into water might be absorbed by and react with periphytic biofilms, which are a kind of aquatic environmental media of important ecological significance, and influence the physiological activity and ecological function of periphytic biofilms. This study investigated the effects of exposure to 1 mg/L and 5 mg/L of TiO₂ NPs on periphytic biofilms cultured indoors. After a 10-day exposure to TiO₂ NPs, the growth (measured by chlorophyll-a content) of microalgal community was inhibited greatly (more than 60%); however, the primary production (indicated by quantum yield) of periphytic biofilms maintained changeless. As for bacteria, TiO₂ NP-exposure increased the bacterial diversity and altered the composition structure. Significant changes were observed in the bacterial communities at the class level, mainly including Alphaproteobacteria, Gammaproteobacteria, Cytophagia, Flavobacteriia, Sphingobacteriia, Synechococcophycideae and Oscillatoriophycideae. The enhancement of metabolic activities (the production of extracellular polymeric substances, especially proteins content increased by 48.51%) of periphytic biofilms was a resistance mechanism to toxicity of NPs. As for extracellular enzyme activities of periphytic biofilms, alkaline phosphatase activity was inhibited (22.43%) after exposed to 5 mg/L of TiO₂ NPs, which posed a threat to phosphorus metabolism of periphytic biofilms. Overall, this study demonstrated that 1 mg/L and 5 mg/L of TiO₂ NPs negatively influenced physiological activities and ecological functions of periphytic biofilms, highlighting that the ecological risks of TiO₂ NPs should be paid attention to.
Afficher plus [+] Moins [-]Benzo[a]pyrene (BaP) exposure generates persistent reactive oxygen species (ROS) to inhibit the NF-κB pathway in medaka (Oryzias melastigma)
2019
Cui, Qian | Chen, Fang-Yi | Chen, Hui-Yun | Peng, Hui | Wang, Ke-Jian
Benzo[a]pyrene (BaP), a common environmental pollutant, can modulate the immune-associated signal pathway NF-κB, which is one of the critical signal pathways involved in various immune responses. BaP exposure usually generates reactive oxygen species (ROS), but whether ROS are predominantly involved in the modulation mechanism of the NF-κB pathway has not been clearly understood. In this study, an in vivo examination of Oryzias melastigma demonstrated that BaP exposure led to a down-regulation of the NF-κB pathway and increased levels of ROS. Conversely, in vitro results using the medaka liver cell line DIT-29 and a widely applied H₂O₂ method showed the opposite: up-regulation of the NF-κB pathway. However, the down-regulation of NF-κB upon BaP exposure in vitro was inhibited by the addition of a ROS inhibitor, indicating ROS are involved in the modulation of NF-κB. The discrepancy between in vivo and in vitro results of ROS impacts on NF-κB activation might be related to the concentration and persistence of ROS. Using a modified luminol detection system, BaP was found to generate sustained physiological concentrations of ROS for 24 h, while an H₂O₂ bolus generated ROS for less than 30 min. Furthermore, a steady-state sub-micromolar H₂O₂ system (H₂O₂ss) was developed in parallel as a positive control of ROS, by which H₂O₂ could be maintained for 24 h. Comparative evaluation using H₂O₂, H₂O₂ss and BaP exposures on the medaka cell line with pGL4.32 demonstrated that the persistent physiological concentrations of ROS generated upon BaP exposure or treatment with H₂O₂ss inhibited the NF-κB pathway, but direct H₂O₂ exposure had the opposite effect. Moreover, a western-blot assay and EMSA detection further confirmed the modulation of the NF-κB pathway in DIT-29. Taken together, this study shows that BaP exposure inhibits the NF-κB pathway by generating sustained physiological concentrations of ROS.
Afficher plus [+] Moins [-]Occurrence and spatial variation of antibiotic resistance genes (ARGs) in the Hetao Irrigation District, China
2019
Shi, Wei | Zhang, Hong | Li, Junjian | Liu, Yong | Shi, Rui | Du, Hongyu | Chen, Jianwen
The prevalence and proliferation of antibiotic resistance genes (ARGs) has been identified as an emerging contaminant of concern and a crucial threat to public health worldwide. To determine the occurrence and distribution of ARGs in artificial agricultural irrigation systems, we designed eight sample sites of farmland drainage in the Hetao Irrigation District, Inner Mongolia, China. Results indicated that the distribution of ARGs in sub-drainage canals is influenced by the local urban area, agriculture, and animal husbandry structure. The blaTEM gene was predominant in the water samples (up to 8.98 ARG copies/16S rRNA genes). The average ARG abundance in drainage channel sampling sites was significantly higher than the influent water from the Yellow River, which means that the artificial agricultural irrigation system enhances the abundance of resistance genes in the study area. Moreover, the effluent water of the whole irrigation system presented a lower abundance of ARGs than the influent water. This demonstrates that the Wuliangsuhai watershed ecosystem plays an important role in regulating the abundance of ARGs in the area. In our study, the mobile gene elements correlated with trB, emrD, mexF, and vanC (P < 0.001) in the irrigation system. Additionally, different correlations exist between other special subtypes of ARGs. These findings provided deeper insights into mitigating the propagation of ARGs and the associated risks to public health.
Afficher plus [+] Moins [-]Denitrification and anammox: Understanding nitrogen loss from Yangtze Estuary to the east China sea (ECS)
2019
Wang, Jing | Kan, Jinjun | Qian, Gang | Chen, Jianfang | Xia, Zhiqiang | Zhang, Xiaodong | Liu, Haijiao | Sun, Jun
The Yangtze River, which is the largest in Euro-Asian, receives tremendous anthropogenic nitrogen input and is typically characterized by severe eutrophication and hypoxia. Two major processes, denitrification and anaerobic ammonium oxidation (anammox), play vital roles for removing nitrogen global in nitrogen cycling. In the current study, sediment samples were collected from both latitudinal and longitudinal transects along the coastal Yangtze River and the East China Sea (ECS). We investigated community composition and distributions of nosZ gene-encoded denitrifiers by high throughput sequencing, and also quantified the relative abundances of both denitrifying and anammox bacteria by q-PCR analysis. Denitrifying communities showed distinct spatial distribution patterns that were impacted by physical (water current and river runoffs) and chemical (nutrient availability and organic content) processes. Both denitrifying and anammox bacteria contributed to the nitrogen removal in Yangtze Estuary and the adjacent ECS, and these two processes shifted from coastal to open ocean with reverse trends: the abundance of nosZ gene decreased from coastal to open ocean while anammox exhibited an increasing trend based on quantifications of hzsB and 16S rRNA genes. Further correspondence correlation analysis revealed that salinity and nutrients were the main factors in structuring composition and distribution of denitrifying and anammox bacteria. This study improved our understanding of dynamic processes in nitrogen removal from estuarine to open ocean. We hypothesize that denitrification is the major nitrogen removal pathway in estuaries, but in open oceans, low nutrient and organic matter concentrations restrict denitrification, thus increasing the importance of anammox as a nitrogen removal process.
Afficher plus [+] Moins [-]Multi-factor identification and modelling analyses for managing large river algal blooms
2019
Xia, Rui | Zhang, Yuan | Wang, Gangsheng | Zhang, Yongyong | Dou, Ming | Hou, Xikang | Qiao, Yunfeng | Wang, Qiang | Yang, Zhongwen
River algal blooms have become a newly emerging global environmental issue in recent decades. Compared with water eutrophication in lakes and reservoirs, algal blooms in large river systems can cause more severe consequences to watershed ecosystems at the watershed scale. However, reveal the causes of river algal blooms remains challenging in the interdisciplinary of hydrological-ecological-environmental research, due to its complex interaction mechanisms impacted by multiple factors. In addition, there were still considerable uncertainties on the characteristics, impacts, driving factors, as well as the applicable water system models for river algal blooms. In this paper, we reviewed existing literature to elaborate the definition and negative effects of river algal blooms. We analyzed sensitive factors including nutrient, hydrological and climatic elements. We also discussed the application of ecohydrological models under complicated hydrological conditions. Finally, we explored the essence of the river algal bloom by the interaction effects of physical and biogeochemical process impacted by of climate change and human activities. The model-data integration accounting for multi-factor effects was expected to provide scientific guidance for the prevent and control of algal blooms in large river systems.
Afficher plus [+] Moins [-]Genomic mutations after multigenerational exposure of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles
2019
Wamucho, Anye | Unrine, Jason M. | Kieran, Troy J. | Glenn, Travis C. | Schultz, Carolin L. | Farman, Mark | Svendsen, Claus | Spurgeon, David J. | Tsyusko, Olga V.
Our previous study showed heritable reproductive toxicity in the nematode Caenorhabditis elegans after multigenerational exposure to AgNO₃ and silver nanoparticles (Ag-NPs). The aim of this study was to determine whether such inheritable effects are correlated with induced germline mutations in C. elegans. Individual C. elegans lineages were exposed for 10 generations to equitoxic concentrations at EC₃₀ of AgNO₃, Ag-NPs, and sulfidized Ag-NPs (sAg-NPs), a predominant environmentally transformed product of pristine Ag-NPs. The mutations were detected via whole genome DNA sequencing approach by comparing F₀ and F₁₀ generations. An increase in the total number of variants, though not statistically significant, was observed for all Ag treatments and the variants were mainly contributed by single nucleotide polymorphisms (SNPs). This potentially contributed towards reproductive as well as growth toxicity shown previously after ten generations of exposure in every Ag treatment. However, despite Ag-NPs and AgNO₃ inducing stronger reproductive toxicity than sAg-NPs, exposure to sAg-NPs resulted in higher mutation accumulation with significant increase in the number of transversions. Thus our results suggest that other mechanisms of inheritance, such as epigenetics, may be at play in Ag-NP- and AgNO₃-induced multigenerational and transgenerational reproductive toxicity.
Afficher plus [+] Moins [-]Dichlorvos alters morphology and behavior in zebrafish (Danio rerio) larvae
2019
Altenhofen, Stefani | Nabinger, Débora Dreher | Bitencourt, Paula Eliete Rodrigues | Bonan, Carla Denise
Dichlorvos (2,2-dichlorovinyl-dimethylphosphate), an organophosphorus pesticide used for indoor insect and livestock parasite control, is among the most common commercially available pesticides. However, there are significant concerns over its toxicity, especially due to its relative stability in water, soil, and air. Zebrafish, an important developmental model, has been used for studying the effects of toxic compounds. The aim of this study was to evaluate the exposure to dichlorvos at early life stages (1 h postfertilization - 7 days postfertilization) in the zebrafish and its toxicological effects during the development, through morphological (7 days postfertilization), locomotor and social behavior analysis (7, 14, 30, 70, and 120 days postfertilization). Dichlorvos (1, 5, and 10 mg/L) exposure reduced the body length and heartbeat rate at 7 days postfertilization (dpf), as well as the surface area of the eyes (5 and 10 mg/L). The avoidance behavior test showed a significant decrease in escape responses at 7 (1, 5, and 10 mg/L) and 14 (5 and 10 mg/L) dpf zebrafish. The evaluation of larval exploratory behavior showed a reduction in distance traveled, mean speed (1, 5, and 10 mg/L) and time mobile (10 mg/L) between control and dichlorvos groups. In addition, the analysis performed on adult animals showed that the changes in distance traveled and mean speed remained reduced in 30 (1, 5, and 10 mg/L) and 70 dpf (5 and 10 mg/L), recovering values similar to the control at 120 dpf. The social behavior of zebrafish was not altered by exposure to dichlorvos in the early stages of development. Thus, the exposure to organophosphorus compounds at early stages of development induces an increased susceptibility to behavioral and neuronal changes that could be associated with several neurodegenerative diseases.
Afficher plus [+] Moins [-]Effects of ingested polystyrene microplastics on brine shrimp, Artemia parthenogenetica
2019
Wang, Ying | Zhang, Dian | Zhang, Mingxing | Mu, Jingli | Ding, Guanghui | Mao, Zheng | Cao, Yifei | Jin, Fei | Cong, Yi | Wang, Lijun | Zhang, Weiwei | Wang, Juying
Microplastics are a contaminant of emerging concern which enter the marine environment from a variety of sources. The ingestion and toxic effects of microplastics on marine life, especially for filter feeders, are a cause of concern in view of their ubiquitous nature and their similar size as food sources. To assess the toxic effects of microspheres ingested by brine shrimp larvae, we exposed Artemia parthenogenetica to 10 μm polystyrene microspheres at different concentrations. These concentrations were approximate to the extrapolated marine aquatic environmentally relevant concentrations. The lowest polystyrene concentrations at which ingestion was visualized in A. parthenogenetica were 12 ± 0.57 particles/mL (6.7 ± 0.32 μg/L) and 1.1 ± 0.16 particles/mL (0.61 ± 0.088 μg/L), respectively. There were no significant impacts on the survival, growth or development in A. parthenogenetica occurring over the 14-d exposure across a range of polystyrene nominal concentrations (1–1000 particles/mL or 0.55–550 μg/L). However, abnormal ultrastructures of intestinal epithelial cells were observed upon exposure to polystyrene microspheres, including fewer and disordered microvilli, an increased number of mitochondrion and the appearance of autophagosome. These phenomena could affect nutrition absorption and energy metabolism. Although no major acute or chronic toxicity effects on A. parthenogenetica were observed over 24-h or 14-d exposures, this study provides evidence that the ingestion of polystyrene microplastics at extrapolated environmentally relevant concentrations can be visualized through a microscope to be causing a series of responses in intestinal epithelial cells.
Afficher plus [+] Moins [-]Compilation and spatio-temporal analysis of publicly available total solar and UV irradiance data in the contiguous United States
2019
Zhou, Ying | Meng, Xia | Belle, Jessica Hartmann | Zhang, Huanxin | Kennedy, Caitlin | Al-Hamdan, Mohammad Z. | Wang, Jun | Liu, Yang
Skin cancer is the most common type of cancer in the United States, the majority of which is caused by overexposure to ultraviolet (UV) irradiance, which is one component of sunlight. National Environmental Public Health Tracking Program at CDC has collaborated with partners to develop and disseminate county-level daily UV irradiance (2005–2015) and total solar irradiance (1991–2012) data for the contiguous United States. UV irradiance dataset was derived from the Ozone Monitoring Instrument (OMI), and solar irradiance was extracted from National Solar Radiation Data Base (NSRDB) and SolarAnywhere data. Firstly, we produced daily population-weighted UV and solar irradiance datasets at the county level. Then the spatial distributions and long-term trends of UV irradiance, solar irradiance and the ratio of UV irradiance to solar irradiance were analyzed. The national average values across all years are 4300 Wh/m², 2700 J/m² and 130 mW/m² for global horizontal irradiance (GHI), erythemally weighted daily dose of UV irradiance (EDD) and erythemally weighted UV irradiance at local solar noon time (EDR), respectively. Solar, UV irradiances and the ratio of UV to solar irradiance all increased toward the South and in some areas with high altitude, suggesting that using solar irradiance as indicator of UV irradiance in studies covering large geographic regions may bias the true pattern of UV exposure. National annual average daily solar and UV irradiances increased significantly over the years by about 0.3% and 0.5% per year, respectively. Both datasets are available to the public through CDC's Tracking network. The UV irradiance dataset is currently the only publicly-available, spatially-resolved, and long-term UV irradiance dataset covering the contiguous United States. These datasets help us understand the spatial distributions and temporal trends of solar and UV irradiances, and allow for improved characterization of UV and sunlight exposure in future studies.
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