In this work PM₂.₅ was collected during winter and summer in a Sardinian village (Gonnostramatza, Italy) highly affected by biomass burning emissions. A multi-technique approach was adopted for the complete PM chemical characterization. The bulk characterization was performed by IC (Ion Chromatography), HPAEC (High-Performance Anion-Exchange Chromatography), TOT (Thermal Optical Transmittance) and ED-XRF (Energy-Dispersive X-Ray Fluorescence) while XPS (X-ray Photoelectron Spectroscopy) was used for the surface characterization. Using levoglucosan as specific tracer of biomass burning emissions, the assessment of the impact of this source was carried out and it represent the major PM source at the investigate site during winter. In winter the average levoglucosan concentration is 2096 ± 324 ng/m³ while during summer its concentration is negligible (18 ± 7 ng/m³). Levoglucosan content in PM₂.₅ during winter is on average 13.7%; it is estimated that 65% of PM₂.₅ is due to wood burning. XPS has been exploited in this work aiming at highlighting possible differences between surface and bulk composition of PM₂.₅. The surface of the particulate matter resulted enriched in carbon compared to the bulk. Among the components of XPS C1s signals recorded on the samples collected during winter, it was found that the signal at 286.5 eV, which is due to the presence of COH, reflects the bulk composition of levoglucosan.

Nickel is a ubiquitous heavy-metal pollutant in lakes and severely affects aquatic organisms. Aquatic plants are often initially linked to having heavy metal contents and further are proposed as phytoremediation agent to remove heavy metal from water. Although the toxic effects of nickel on aquatic plants are thoroughly explored, the effective investigation to increase Ni tolerance is still in its infancy. The role of rare-earth elements (REEs) in plant resisting heavy-metal pollution has recently received considerable interest. To explore the physiological effects of REEs on Potamogeton crispus under Ni stress, we explored whether or not the additive exposure to low-dose yttrium (Y; 2.5 μM) promotes the polyamine metabolism, antioxidation, and photosynthesis performance of P. crispus under Ni stress values of 0, 50, 100, 150, and 200 μM. Results showed that Y exposure did not influence Ni bioaccumulation in P. crispus. Furthermore, Y exposure alleviated the adverse effects of Ni stress to convergent degrees because Y positively converts putrescine into spermidine and spermine, inhibits oxidative stress, increases the total chlorophyll content, and maximum/potential quantum efficiency of photosystem II. We concluded that low-dose Y can positively regulate polyamine transformation, inhibit oxidative stress, stimulate photosynthesis, and finally promote the resist ability of P. crispus to nickel stress. Thus, REEs have potential to be applied in regulating submerged plant tolerance to aquatic heavy-metal pollution.

Although agricultural lands are generally assumed to correlate negatively with groundwater quality, the intricate relationship between general land cover and contaminant concentrations present in an aquifer may vary substantially; contingent upon the land type, interacting factors, and scale considered. The Upper Floridan Aquifer (UFA) is a primary source of potable water supply for the state of Florida. The Suwannee River Water Management District (SRWMD), located in northcentral Florida, relies exclusively on the UFA for water supplies. Over much of the SRWMD in the UFA is unconfined, rendering it vulnerable to contamination from surface sources. This study analyses groundwater concentrations of Nitrate-Nitrogen (NO3–N) and Potassium (K) from shallow wells across the SRWMD for assessing the effect of different land covers on groundwater quality over time. Annual potentiometric surface maps were used to delineate semicircular recharge zones of 500 m, 1000 m, and 2000 m radii upstream of sampled well stations. Proportions of agriculture, forest, and urban lands were identified for each buffer zone using USDA Cropland Data Layer. Multivariate regression models were developed to infer relationships between land cover and NO3–N and K concentrations. Results show significant associations among land cover type, water table height, and groundwater quality parameters. Specifically, we find a large proportion of agricultural cover consistently associated with larger increases in groundwater pollutant loads relative to urban or forest cover across all models, after controlling for depth to water table. Our study suggests a need for widespread adoption of cost-effective agricultural best management practices (BMPs) that could help in securing regional water supply.

Parabens are extensively applied in cosmetics, drugs or food as preservatives and have become common pollutants in environmental media. However, data on human exposure to these chemicals is still limited, especially for children. This study aimed to investigate parabens in urine samples of children and to evaluate the cumulative risk of paraben exposure. Five short-chain parabens were measured in 255 urine samples collected from children in a kindergarten and elementary schools from South China. Methyl paraben (MeP), ethyl paraben (EtP) and n-propyl paraben (PrP) were widely detected in urine samples (detection rates > 94.9%), indicating their widespread exposure. The urinary median concentrations of MeP, EtP and PrP were 2.25, 0.33 and 0.50 μg/L, respectively. Significantly positive correlations (p < 0.01) were observed between MeP and PrP in urine, suggesting similar sources and/or metabolic pathways of these two chemicals. The median estimated daily intakes (EDIs) of parabens were determined to be 18.1 and 9.79 μg/kg-bw/day for kindergarten children and elementary school students, respectively. Estimation of human intake and exposure risks indicated potential risks of PrP exposure for elementary school students. This is the first study addressing paraben exposure in South China children.

Aflatoxin B₁ (AFB₁) is a hazard environmental pollutants and the most toxic one of all the aflatoxins. AFB₁ can cause a serious impairment to testicular development and spermatogenesis, yet the underlying mechanisms remain inconclusive. Oxidative stress acts as a master mechanism of AFB₁ toxicity, and can promote autophagy. Abnormal autophagy resulted in testicular damage and spermatogenesis disorders. The objective of this study was to explore the effect of AFB₁ on autophagy in mice testis and its potential mechanisms. In this study, male mice were intragastrically administered with 0, 0.375, 0.75 or 1.5 mg/kg body weight AFB₁ for 30 days. We found that AFB₁ induced testicular damage, reduced serum testosterone level and impaired sperm quality accompanied with the elevation of oxidative stress and germ cell apoptosis. Interestingly, we observed increasing numbers of autophagosomes in AFB₁-exposed mice testis. Meanwhile, AFB₁ caused testis abnormal autophagy with the characterization of increased expressions of LC3, Beclin-1, Atg5 and p62. Furthermore, AFB₁ downregulated the expressions of PI3K, p-AKT and p-mTOR in mice testis. Taken together, our data indicated AFB₁ induced testicular damage and promoted autophagy, which were associated with oxidative stress-related PI3K/AKT/mTOR signaling pathway in mice testis.

Microplastics can adsorb chemical pollutants such as metals or pharmaceuticals, and transferred them along the food chain. In this work, an investigation of the adsorption of Cd, Co, Cr, Cu, Ni, Pb and Zn by five different types of microplastics was performed in Milli-Q water and natural waters (seawater, urban wastewater and irrigation water) via a series of batch adsorption experiments. The effects of concentration of metals and physicochemical characteristics of polymers were particularly studied. Results revealed a significant adsorption of lead, chromium and zinc on microplastics, especially on polyethylene and polyvinyl chloride. In the case of polyethylene terephthalate, it showed little adsorption capacity. Specific surface, porosity and morphology are characteristics that affect the molecular interactions. The adsorption isotherms were better described by Langmuir model, which indicates that the main adsorption mechanism might be chemical adsorption. Finally, results obtained in natural waters indicated that dissolved organic matter may play a major role on metal adsorption on microplastics. Results showed an enhancement of metal adsorption in waters with high chemical and biological oxygen demands as urban wastewater and irrigation water.

The present study evaluated the river water quality improvement by implementation of household-based biodigesters in vulnerability and poverty rural area, in Minas Gerais State-Brazil. For that, 78 household-based biodigesters were installed for domestic wastewater treatment. Wastewater was collected before and after treatment and the physicochemical parameters and pathogens removal (human adenovirus (HAdV), hepatitis A (HAV) virus, Salmonella sp. and Escherichia coli) were evaluated; Additionally, river water was sampled before and after the household-based biodigesters implementation, to verify the contamination reduction and the positive impact of domestic wastewater treatment on waterborne pathogen reduction, considering HAdV, HAV, Salmonella sp. and E. coli quantification. The applicability in real-scale of decentralized treatment systems using household-based biodigesters promoted reduction of 90, 99, 99.99 and 99.999% from HAV, Salmonella sp., E. coli and HAdV from domestic wastewater, respectively; The river water quality improvement before the wastewater treatment application was highlight in the present study, considering that the reduction of waterborne pathogens in this water in 90, 99.99 and 99.999% of E. coli, HAV and HAdV, respectively (Salmonella sp. was not detected in river water). In general, this is an important study for encouraging the decentralized sanitation in vulnerable and poverty area, as well in rural sites, considering the positive impact of this implementation on public health.

In order to exhaustively investigate the physical and chemical mechanisms of heavy metal immobilization in sludge incineration residue (SIR)-based magnesium potassium phosphate cement (MKPC), this work investigated the influence of Pb²⁺ and Zn²⁺ on the compressive strength and microstructure of SIR-based MKPC, and the efficiency of Pb and Zn immobilization. Taking the difference of Ksp (solubility product) of different heavy metal compounds as the entry point, the physical and chemical mechanisms of Pb and Zn immobilization, and the competitive mechanism between coexisting ions, were comprehensively analyzed. It was discovered that Pb²⁺ is in the form Pb₃(PO₄)₂, and Zn²⁺ is immobilized in the form Zn₂(OH)PO₄ [Zn₃(PO₄)₂ is preferentially formed, when the pH > 7, Zn₃(PO₄)₂ is converted to Zn₂(OH)PO₄]. The low solubility of heavy metal phosphates is the main reason that Pb²⁺ and Zn²⁺ are well immobilized. The preferential formation of Pb₃(PO₄)₂ (Kₛₚ = 8 × 10⁻⁴³) and Zn₃(PO₄)₂ (Kₛₚ = 9.0 × 10⁻³³) reduced the amount of MgKPO₄·6H₂O (Kₛₚ = 2.4 × 10⁻¹¹), resulting in a decrease in compressive strength. Besides, coexisting Pb²⁺ and Zn²⁺ has a competitive effect: Pb²⁺ will weaken the immobilization efficiency of Zn²⁺. The new exploration of these mechanisms provide a theoretical basis for rationally adjusting the Magnesia/Phosphate ratio to enhance the compressive strength and improve the efficiency of heavy metals immobilization.

As a new type of emerging pollutant in the ocean, microplastics have received global attention in recent years. Considering the increasing amount of human activities around the South China Sea, it is important to determine the current status of microplastic pollution in this region. In this study, we analyzed the abundance and distribution of microplastics at Zhubi Reef in the South China Sea. Microplastic abundance ranged from 1,400 to 8,100 items/m3 of surface water, which was much higher than the values reported from other ocean areas. About 80% of the microplastics were smaller than 0.5 mm in size. Fibers and pellets comprised the most common microplastic types. The dominant microplastics were transparent or blue in color. The main polymer types were polypropylene (25%) and polyamide (18%). In general, our results revealed Zhubi Reef was contaminated with microplastics, which were likely derived from the intensive fisheries in the area and emissions from coastal cities. This study also provides baseline data that are useful for additional studies of microplastics in the South China Sea.