Thiacloprid is a neonicotinoid insecticide widely exploited in agriculture and easily mobilized towards aquatic environments by atmospheric agents. However, little information about its toxicological effects on aquatic invertebrate bioindicators is available. In this study, specimens of the mussel Mytilus galloprovincialis were exposed to thiacloprid at environmental (4.5 μg L⁻¹) and 100 times higher than environmental (450 μg L⁻¹) concentrations for 20 days. Thiacloprid affected haemolymph biochemical parameters, cell viability in the digestive gland, antioxidant biomarkers and lipid peroxidation in the digestive gland and gills at environmentally relevant concentrations (4.5 μg L⁻¹). In addition, thiacloprid exposure caused histological damage to the digestive gland and gills. Interestingly, the pesticide was detected at levels equal to 0.14 ng g⁻¹ in the soft tissues of sentinels exposed for 20 days to 450 μg L⁻¹ thiacloprid in seawaterμ. Due to its harmful potential and cumulative effects after long-term exposure of M. galloprovincialis, thiacloprid may pose a potential risk to nontarget aquatic organisms, as well as to human health. This aspect requires further in-depth investigation.

Agricultural activities often lead to nutrient enrichment and habitat modification in rivers, possibly altering macroinvertebrate assemblages and hence ecosystem functioning. For the sake of environmental management and conservation, therefore, assessing the impacts of agricultural activities becomes indispensable, especially when these activities are predicted to be intensified in the future. In this study, the plain river network in the Lake Chaohu Basin was chosen to examine how agricultural activities influence the functioning of rivers by assessing land use, water quality, habitat condition and macroinvertebrate assemblages, followed by calculating the macroinvertebrate-based multimetric index (MMI) to indicate overall ecosystem health of the rivers. We found that agricultural activities lowered the diversity of macroinvertebrates (e.g. total number of taxa and Simpson index) primarily due to elevated ammonium concentrations in water and reduced microhabitat types, thereby impairing the habitat integrity and nutrient cycling of rivers. The macroinvertebrate-based MMI was positively correlated with increasing habitat quality but negatively with increasing nutrient concentrations, suggesting its high reliability for indicating the impacts of agricultural activities, which was further substantiated by classification and regression tree (CART) analysis. We recommend analyzing macroinvertebrate assemblages using both multivariate and multimetric approaches to offer a more comprehensive evaluation of the impacts of agricultural activities on ecosystem health. Some environmental (CODMₙ, NH₄⁺-N and PO₄³⁻-P) and biological parameters (total number of taxa), however, can be used as good proxies for MMI, when time and resources for gathering information to develop MMI are limited.

Seabirds are widely used as indicators of marine pollution, including mercury (Hg), because they track contaminant levels across space and time. However, many seabirds are migratory, and it is difficult to understand the timing and location of their Hg accumulation. Seabirds may obtain Hg thousands of kilometers away, during their non-breeding period, and deposit that Hg into their terrestrial breeding colonies. We predicted that Hg concentration in rectrices reflects exposure during the previous breeding season, in body feathers reflects non-breeding exposure, and in blood collected during breeding reflects exposure during current breeding. To test this hypothesis, we measured total Hg concentration in these three tissues, which reflect different timepoints during the annual cycle of rhinoceros auklets (Cerorhinca monocerata) breeding on both sides of the North Pacific (Middleton Island in Alaska and Teuri Island in Hokkaido), and tracked their wintering movement patterns with biologging devices. We (i) identify the wintering patterns of both populations, (ii) examine Hg levels in different tissues representing exposure at different time periods, (iii) test how environmental Hg exposure during the non-breeding season affects bird contamination, and (iv) assess whether variation in Hg levels during the non-breeding season influences levels accumulated in terrestrial plants. Individuals from both populations followed a figure-eight looping migration pattern. We confirm the existence of a pathway from environmental Hg to plant roots via avian tissues, as Hg concentrations were higher in plants within the auklet colonies than at control sites. Hg concentrations of breast feathers were higher in Alaskan than in Japanese auklets, but Hg concentrations in rectrices and blood were similar. Moreover, we found evidence that tissues with different turnover rates could record local anthropogenic Hg emission rates of areas visited during winter. In conclusion, Hg was transported across thousands of kilometers by seabirds and transferred to local plants.

Poly- and perfluoroalkyl substances (PFAS) have become ubiquitous contaminants in the environment. Contamination of the terrestrial ecosystem can occur from the release of aqueous film forming foams (AFFF) used in firefighting operations. Following soil contamination with AFFF, studies report root uptake and translocation of PFAS to other plant organs, typically favouring the short chain moiety. This body of experimental work often focuses on edible organs and generally lacks entire PFAS budgets. Here, we calculate short chain (≤6 carbons) and long chain (≥6 or ≥ 7 carbons) PFAS concentrations and respective budgets for terrestrial multimedia mesocosms (plants, soil and lysimeter) of three common agricultural plants (tomato, lettuce and beet) following irrigation with low level PFAS (<1 μg L⁻¹) contaminated river water (short chain: 167 ng L⁻¹; long chain 526 ng L⁻¹). Total net recoveries were strong, ranging between 91% and 118% of added PFAS across all media. While soil was the largest receptor of PFAS in general (∼70% and 115%), there was considerable mobility to various media, including vegetation (∼3% and 20%) and leachate (∼1%). Translocation of short chain PFAS to tomato flowers resulted with biomagnified concentrations (maximus >4000 ng g⁻¹) and accounted for 1.4% of PFAS additions. While smaller tomato fruits had higher concentrations of short chain PFAS, larger fruit had more total PFAS mass. This work provides a detailed description of the fate of short and long chain PFAS when added to relatively uncontaminated terrestrial agricultural systems. We show low-level PFAS concentrations from real-world irrigation sources can affect various receptors across the multimedia landscape. This is most evident in tomato flowers and fruit where biomagnification and high total masses of short chain PFAS occurred which could have implications for pollinators and consumption, respectively.

The response of freshwater invertebrates following accidental releases of oil is not well understood. This knowledge gap is more substantial for unconventional oils such as diluted bitumen (dilbit). We evaluated the effects of dilbit on insect emergence and benthic invertebrates by conducting experimental spills in limnocorrals (10-m diameter; ~100-m³) deployed in a boreal lake at the IISD-Experimental Lakes Area, Canada. The study included seven dilbit treatments (spill volumes ranged from 1.5 L [1:66,000, oil:water, v/v] to 180 L [1:590, oil:water, v/v]), two controls, and additional lake reference sites, monitored for 11 weeks. Invertebrate emergence declined at the community level following oil addition in a significantly volume-dependent manner, and by 93–100 % over the 11 weeks following the spill in the highest treatment. Dilbit altered community structure of benthic invertebrates, but not abundance. One-year post-spill and following oil removal using traditional skimming and absorption techniques, benthic richness and abundance were greater among all treatments than the previous year. These results indicate that recovery in community composition is possible following oil removal from a lake ecosystem. Research is needed concerning the mechanisms by which surface oil directly affect adult invertebrates, whether through limiting oviposition, limiting emergence, or both. The response of benthic communities to sediment tar mats is also warranted.

Chlorinated paraffins (CPs), a type of toxic and persistent organic substances, can persist in environmental media for a long time and have adverse effects on human health. Thus, it is of great importance to investigate the accumulation and environmental behavior of CPs in industrial areas. In this study, farmland soil, water, and sediment core samples from abandoned oxidation ponds used by three chemical plants to treat wastewater over the past 38 years were investigated in detail. Results show that the concentration of CPs in sediments varied significantly with the water flow direction. The oxidation pond closest to a sewage outlet had the highest concentrations of short-chain chlorinated paraffin (SCCPs) and medium-chain chlorinated paraffin (MCCPs), within the ranges of 44.0–6.21 × 10⁴ ng/g dw (mean 9.32 × 10³ ng/g dw) and 143–1.30 × 10⁶ ng/g dw (mean 1.22 × 10⁵ ng/g dw), respectively. However, in the oxidation pond farthest from the sewage outlet, CP concentrations in sediments were significantly reduced, with ∑SCCPs and ∑MCCPs concentrations ranging from N.D.-249 ng/g dw (mean 66.8 ng/g dw) and N.D.-222 ng/g dw (mean 34.0 ng/g dw), respectively. Moreover, MCCP level in the water was below the detection limit, while the concentration of SCCP ranged from 41.0 to 1.53 × 10³ ng/L (mean 267 ng/L). Finally, a remarkable spatial trend and specific congener distribution were observed in the sediment test results. The horizontal and vertical distributions of the sediments indicate that short-chain (C₁₀₋₁₁) and low-chlorinated (Cl₆₋₇) homologs are more likely to migrate deeper or farther away from the pollution source.

Insecticidal Cry proteins from Bacillus thuringiensis (Bt) can be transferred from genetically engineered crops to herbivores to natural enemies. For the lady beetle Harmonia axyridis, we investigated potential uptake of Cry proteins from the gut to the body and intergenerational transfer. Third and fourth instar H. axyridis fed with pollen or spider mites from SmartStax maize contained substantial amounts of Cry1A.105, Cry1F, Cry2Ab2, Cry3Bb1, and Cry34Ab1. Cry protein concentrations in lady beetle larvae were typically one order of magnitude lower than in the food. When H. axyridis larvae were fed Bt maize pollen, median amounts of Cry protein in the non-feeding pupae were below the limit of detection except for small amounts of Cry34Ab1. No Cry protein was detected in pupae when spider mites were used as food. Cry protein concentrations decreased quickly after H. axyridis larvae were transferred from pollen or spider mites to Bt-free food. Aphids contained very low or no detectable Cry protein, and no Cry protein was found in H. axyridis larvae fed with aphids, and in pupae. When H. axyridis adults were fed with Bt maize pollen (mixed with Ephestia kuehniella eggs), the median concentrations of Cry proteins in lady beetle eggs were below the limit of detection except for Cry34Ab1 in eggs laid later in adult life. No Bt protein was detected in eggs laid by H. axyridis females fed with aphids from Bt maize. Our results confirm previous observations that Cry proteins are degraded and excreted quickly in the arthropod food web without evidence for bioaccumulation. Despite the fact that small amounts of Cry proteins were detected in some samples of the non-feeding pupal stage of H. axyridis as well as in eggs, we conclude that this route of exposure is unlikely to be significant for predators or parasitoids in a Bt maize field.

Microorganisms able to form biofilms in marine ecosystems are selected depending on immersed surfaces and environmental conditions. Cell attachment directly on toxic surfaces like antifouling coatings suggests a selection of tolerant (or resistant) organisms with characteristics conferring adaptive advantages. We investigated if environment would drive metal resistance gene abundance in biofilms on artificial surfaces. Biofilms were sampled from three surfaces (a PVC reference and two antifouling coatings) deployed in three coastal waters with dissimilar characteristics: The Mediterranean Sea (Toulon) and Atlantic (Lorient) and Indian (Reunion) Oceans. The two coatings differed in metals composition, either Cu thiocyanate and Zn pyrithione (A3) or Cu2O (Hy). Metal resistance genes (MRG) specific to copper (cusA, copA, cueO) or other metals (czcA and pbrT) were monitored with qPCR in parallel to the microbial community using 16S rRNA gene metabarcoding. A lower α-diversity on A3 or Hy than on PVC was observed independent on the site. Weighted Unifrac suggested segregation of communities primarily by surface, with lower site effect. Metacoder log2 fold change ratio and LeFSe discrimination suggested Marinobacter to be specific of Hy and Altererythrobacter, Erythrobacter and Sphingorhabdus of A3. Likewise, the relative abundance of MRG (MRG/bacterial 16S rRNA) varied between surfaces and sites. A3 presented the greatest relative abundances for cusA, cueO and czcA. The latter could only be amplified from A3 communities, except at Toulon. Hy surface presented the highest relative abundance for copA, specifically at Lorient. These relative abundances were correlated with LeFSe discriminant taxa. Dasania correlated positively with all MRG except cueO. Marinobacter found in greater abundance in Hy biofilm communities correlated with the highest abundances of copA and Roseovarius with czcA. These results prove the selection of specific communities with abilities to tolerate metallic biocides forming biofilms over antifouling surfaces, and the secondary but significant influence of local environmental factors.

The present research is mainly focusing on the characterization of X-ray developer solution and its toxic tolerance studies with Desmodesmus armatus towards the phycoremediation studies for removal of pollutants, silver, and concomitant lipid production. The characterization results suggested the presence of 1.229 ± 0.004 g/l BOD, 27.29 ± 0.230 g/l COD with a silver content of 0.01791 ± 0.000 g/l. The tolerance and toxicity limits of with X-ray developer solution reveals the remarkable growth of microalgae in 3:1.dilution ratio of BBM in the X-ray developer solutions. The phycoremediation with 19 days period shown the noticeable results with a relative BOD (20.86%), COD (13.88%), with 57.10% corresponding total phosphorous removal. The phycoremediation also has proven better relative silver removal potential of 44.06% on the 19th day with concomitant 1.392% lipid production. Overall, the present study shows the potential phycoremediation strategy of hazardous X-ray developer solutions with possible concurrent lipid production through a sustainable approach.

Pathogenic survivals were dramatically affected by Fe³⁺ and Mn²⁺ under freeze-thaw (FT), and the dissolutions of manganese and iron oxides (MIOs) were also accelerated under FT. But the mutual influences of pathogenic bacterial survival and MIOs under FT have not been profoundly explored yet. In this work, aqueous systems containing Escherichia coli as well as synthetic ferrihydrite (Fh) and manganese dioxide (MnO₂) were experimented under simulated FT cycles to study the mutual influences of metal oxides and bacteria survival while oxide dissolutions and appearances, bacterial morphology and activities (survival number, cell surface hydrophobicity (CSH) and superoxide dismutase (SOD)) were obtained. The results showed that broken E. coli cells by ice growth were observed, but both oxides promoted E. coli survival under FT stress and prolonged bacterial survival time by 1.2–2.9 times, which were mainly attributed to the release of Fe³⁺ and Mn²⁺ caused by FT. The dissolutions of Fh and MnO₂ under FT, which took place at a low level in absence of E. coli cells, were markedly enhanced with bacterial interferences by 2–8 times and higher dissolved manganese concentrations were detected than iron. This was probably because that concentrated organic matters which were released from broken cells, rejected into unfrozen liquid layer and acted as electron donors and ligands to oxide dissolution. Compared with Fh system, more significant promotion of E. coli survival under FT in MnO₂ systems were found because of more SOD generations associated with high dissolved manganese concentrations and the stronger cellular protection by MnO₂ aggregations. The results suggested that FT significantly influenced the interactions between metal oxides and bacterial in water, resulting to changes in pathogen activity and metal element cycling.