The impact of CeO₂ nanoparticles (NPs) on plant physiology and soil microcosm and the underlying mechanism remains unclear to date. This study investigates the effect of CeO₂ NPs on plant growth and soil microbial communities in both the rhizosphere of cucumber seedlings and the surrounding bulk soil, with CeCl₃ as a comparison to identify the contribution of the particulate and ionic form to the phytotoxicity of CeO₂ NPs. The results show that Ce was significantly accumulated in the cucumber tissue after CeO₂ NPs exposure. In the roots, 5.3% of the accumulated Ce has transformed to Ce³⁺. This transformation might take place prior to uptake by the roots since 2.5% of CeO₂ NPs was found transformed in the rhizosphere soil. However, the transformation of CeO₂ NPs in the bulk soil was negligible, indicating the critical role of rhizosphere chemistry in the transformation. CeO₂ NPs treatment induced oxidative stress in the roots, but the biomass of the roots was significantly increased, although the Vitamin C (Vc) content and soluble sugar content were decreased and mineral nutrient contents were altered. The soil enzymatic activity and the microbial community in both rhizosphere and bulk soil samples were altered, with rhizosphere soil showing more prominent changes. CeCl₃ treatment induced similar effects although less than CeO₂ NPs, suggesting that Ce³⁺ released from CeO₂ NPs contributed to the CeO₂ NPs induced impacts on soil health and plant physiology.

Nanosilver (NAg) is currently one of the major alternative antimicrobials to control microorganisms. With its broad-spectrum efficacy and lucrative commercial values, NAg has been used in medical devices and increasingly, in consumer products and appliances. This widespread use has inevitably led to the release and accumulation of the nanoparticle in water and sediment, in soil and even, wastewater treatment plants (WWTPs). This Article describes the physical and chemical transformations of NAg as well as the impact of the nanoparticle on microbial communities in different environmental settings; how the nanoparticle shifts not only the diversity and abundance of microbes, including those that are important in nitrogen cycles and decomposition of organic matters, but also their associated genes and in turn, the key metabolic processes. Current findings on the microbiological activity of the leached soluble silver, solid silver particulates and their respective transformed products, which underpin the mechanism of the nanoparticle toxicity in environmental microbes, is critically discussed. The Article also addresses the emerging evidence of silver-driven co-selection of antibiotic resistance determinants. The mechanism has been linked to the increasing pools of many antibiotic resistance genes already detected in samples from different environmental settings, which could ultimately find their ways to animals and human. The realized ecological impact of NAg calls for more judicial use of the nanoparticle. The generated knowledge can inform strategies for a better ‘risks versus benefits’ assessment of NAg applications, including the disposal stage.

Aquatic organisms are exposed to complex mixtures of pesticides in the environment, but traditional risk assessment approaches typically only consider individual compounds. In conjunction with exposure to pesticide mixtures, global climate change is anticipated to alter thermal regimes of waterways, leading to potential co-exposure of biota to elevated temperatures and contaminants. Furthermore, most studies utilize aqueous exposures, whereas the dietary route of exposure may be more important for fish owing to the hydrophobicity of many pesticides. Consequently, the current study aimed to determine the effects of elevated temperatures and dietary pesticide mixtures on swimming performance and lipid metabolism of juvenile Chinook salmon, Oncorhynchus tshawytscha. Fish were fed pesticide-dosed pellets at three concentrations and three temperatures (11, 14 and 17 °C) for 14 days and swimming performance (Uₘₐₓ) and expression of genes involved in lipid metabolism and energetics were assessed (ATP citrate lyase, fatty acid synthase, farnesoid x receptor and liver x receptor). The low-pesticide pellet treatment contained five pesticides, p,p’-DDE, bifenthrin, esfenvalerate, chlorpyrifos and fipronil at concentrations based on prey items collected from the Sacramento River (CA, USA) watershed, with the high-pesticide pellet treatment containing a six times higher dose. Temperature exacerbated effects of pesticide exposure on swimming performance, with significant reductions in Uₘₐₓ of 31 and 23% in the low and high-pesticide pellet groups relative to controls at 17 °C, but no significant differences in Uₘₐₓ among pesticide concentrations at 11 or 14 °C. At 14 °C there was a significant positive relationship between juvenile Chinook salmon pesticide body residues and expression of ATP citrate lyase and fatty acid synthase, but an inverse relationship and significant downregulation at 17 °C. These findings suggest that temperature may modulate effects of environmentally relevant pesticide exposure on salmon, and that pesticide-induced impairment of swimming performance may be exacerbated under future climate scenarios.

Rare earth elements inevitably release into the soil due to their widespread application. However, it is unclear how they affect the soil animals. The study surveyed the growth and physiological responses of earthworm (Eisenia fetida) exposed into artificial soils spiked with La, Ce, and their mixture, and actual mine soil collected from an abandoned La–Ce mining area (Mianning, Sichuan). The results showed that the 1000–1200 mg/kg combined exposure in two soils induced significant histopathological and phenotypic changes of earthworms. Concentration significantly affected the superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA), and protein of E. fetida and the effects differentiated with the prolonging duration. These indicators were negatively affected under the La stress ≥800 mg/kg (SOD, POD, and protein), the 1200 mg/kg (SOD), Ce stress ≥1000 mg/kg (protein), and the combination ≥800 mg/kg (SOD, POD) and ≥1000 mg/kg (protein). Artificial combination had −15.04% (SOD), 8.87% (POD), 5.64% (MDA), and −8.34% (protein) difference compared with the contamination soil, respectively. Overall, E. fetida respond sensitively under the La and Ce stress, the antioxidant defense system and the lipid peroxidation were stimulated, and the artificial soil might overestimate eco-toxicological effect.

Lichens play an important role in the biogeochemical cycling of mercury (Hg) and are commonly used as indicators of Hg enrichment in remote and anthropogenically impacted environments. To assess their capacity for Hg uptake and accumulation, we determined the concentration of gaseous elemental mercury (GEM) in air and the concentration of total Hg (THg) in transplanted thalli of two lichen species. Lichen transplants and passive air samplers (PASs) were concurrently deployed, side by side, at 10 sites within an abandoned mining area, characterized by large gradients in atmospheric Hg contamination. Highly variable time-weighted GEM concentrations determined by the PASs, ranging from 17 to 4,200 ng/m³, were mirrored by generally high Hg concentrations in transplanted thalli of both Xanthoria parietina (174–8,800 ng/g) and Evernia prunastri (143–5,500 ng/g). Hg concentrations in the two species co-varied linearly indicating about 60% greater Hg accumulation in X. parietina than in E. prunastri. Whereas Hg uptake in the fruticose E. prunastri increased linearly with GEM, a power law equation with a fractional exponent described the uptake in the foliose X. parietina. Extrapolating the relationships observed here to higher GEM levels yielded concentrations in lichen that agree very well with those measured in an earlier fumigation experiment performed under laboratory-controlled conditions. The uptake model of X. parietina was further verified by correctly estimating GEM concentrations from the THg measured in autochthonous thalli collected from the urban area adjacent to the mine site. Passive sampling can effectively provide time-weighted data of suitable spatial resolution to quantitatively describe GEM assimilation by lichens. Therefore, the combined use of passive sampling and lichen transplants can contribute to a more comprehensive understanding of the role of lichens, and potentially also of other cryptogams, in the deposition of atmospheric Hg to terrestrial ecosystems.

Bisphenol A (BPA) is widely used by manufacturers and in consumer products. Its release in the environment may affect male reproductive function. In this study, we examined the effect of low dose (0.1 mg/kg BW), short term exposure during puberty (PD21-35) on adult rat male reproduction. The results indicated that such exposure reset growth hormone (GH) and follicular stimulating hormone (FSH) homeostasis and resulted in a significantly higher level of serum testosterone without affecting serum luteinizing hormone level. QPCR and Western blot results showed that BPA significantly up-regulated selective genes/proteins in the Leydig cell steroidogenic pathway, including steroidogenic acute regulatory protein, cytochrome P450 11A1, cytochrome P450 17A, and low-density lipoprotein receptor. RNA-Seq analysis of testicular RNAs showed that BPA significantly affected the gene profiles of multiple testicular interstitial populations without affecting germ cells. Also, GO- and KEGG-analysis suggested that IGF1-related PI3K/AKT signaling was activated, which was confirmed by the increased phosphorylation of IRS1, AKT1 and CREB. The results indicated that a low-dose, short-term BPA exposure during puberty affected the adult male rat pituitary (GH and FSH) and testis (testosterone) homeostasis.

Microplastics (<5 mm) are divided into primary and secondary microplastics, which are further degraded into nanoplastics. The microplastic particles are widely distributed in marine environment, terrestrial ecosystem and biological organism, leading to damages to whole environmental system. Microplastics are not only difficult to degrade, but also able to adsorb pollutants. Due to the tiny size and various properties, the separation and characterization of microplastic particles has become more and more challenging. This review introduces the sources and destinations of the microplastic particles and summarizes the general methods for the sorting and characterization of microplastics, especially the manipulation of microplastic particles on microfluidic chip, showing possibility to deal with smaller nanoplastic particles over traditional methods. This review focuses on studies of the size-based separation and property-dependent characterization of microplastics in marine environment by utilizing the microfluidic chip device.

1,2-Dichloroethane (1,2-DCE) is a highly toxic neurotoxicity, and the brain tissue is the main target organ. At present, long-term exposure to 1,2-DCE has been shown to cause cognitive dysfunction in some studies, but the mechanism is not clear. The results of this study showed that long-term 1,2-DCE exposure decreased learning and memory abilities in mice and impaired the structure and morphology of neurons in the hippocampal region. Moreover, except for the mRNA level of PAG, the enzymatic activities and protein levels of GS and PAG, as well as the mRNA level of GS were inhibited. With increasing dose of exposure, the protein and mRNA expression of GLAST and GLT-1 also decreased. Contrarily, there were protein and mRNA expression upregulation of GluN1, GluN2A and GluN2B in the hippocampus, as well as increased levels of extracellular Glu and intracellular Ca²⁺. In addition, 1,2-DCE exposure also downregulated the protein expression levels of CaM, CaMKII and CREB. Taken together, our results suggest that long-term 1,2-DCE exposure impairs the learning and memory capacity in mice, which may be attributed to the disruption of Glu metabolism and the inhibition of CaM- CaMKII-CREB signaling pathway in the hippocampus.

NH₃ emission control has proven to be of great importance in reducing PM₂.₅ concentrations in China, while how it affects nitrogen/sulfur (N/S) deposition is still unclear. This study expanded the response surface model method to quantify the responses of N/S deposition to the emission control of precursors (NOₓ, SO₂, NH₃, VOCs and primary PM₂.₅) in the Yangtze River Delta, China. NH₃ control was found to have higher efficiency in reducing N/S deposition than NOₓ and SO₂ alone. The reduced N deposition response to NH₃ emission control was higher in the northern part of the YRD region, whereas oxidized N deposition decreased sharply in the region with a low N critical load. Synergetic effect was found in reducing N deposition when we controlled the NH₃ and NOₓ emissions simultaneously. Compared with the sum effect of individual NH₃ and NOₓ emission control, the extra benefits from the synergy controls accounted for 4.4% (1.23 kg N·ha⁻¹·yr⁻¹) of the total N deposition, of which 81% came from the oxidized N deposition. The YRD region could receive the largest synergetic effect with a 1:1 ratio of NOₓ:NH₃ emission reduction. The NH₃ emission control increases the dry deposition of acid substances and worsens acid rain though it reduces the wet S/oxidized N deposition. These findings highlight the effectiveness of NH₃ emission control and suggest a multi-pollutant control strategy for reducing N/S deposition. The response surface model method for deposition also provides a reference for other regions in China and other countries.

Oil pollution remains a prominent local hazard to coral reefs, but the sensitivity of some coral life stages to oil exposure remains unstudied. Exposure to ultraviolet radiation (UVR), ubiquitous on coral reefs, may significantly increase oil toxicity towards these critical habitat-forming taxa. Here we present the first data on the sensitivity of two distinct post-settlement life stages of the model coral species Acropora millepora to a heavy fuel oil (HFO) water accommodated fraction (WAF) in the absence and presence of UVR. Assessment of lethal and sublethal endpoints indicates that both 1-week-old and 2-month-old recruits (1-wo and 2-mo) were negatively affected by chronic exposures to HFO (7 and 14 days, respectively). Relative growth (1-wo and 2-mo recruits) and survival (1-wo recruits) at end of exposure were the most sensitive endpoints in the absence of UVR, with no effect concentrations (NEC) of 34.3, 5.7 and 29.3 μg L⁻¹ total aromatic hydrocarbons (TAH; ∑39 monocyclic- and polycyclic aromatic hydrocarbons), respectively. On average, UVR increased the negative effects by 10% for affected endpoints, and latent effects of exposure were evident for relative growth and symbiont uptake of recruits. Other sublethal endpoints, including maximum quantum yield and tissue colour score, were unaffected by chronic HFO exposure. A comparison of putative species-specific sensitivity constants for these ecologically relevant endpoints, indicates A. millepora recruits may be as sensitive as the most sensitive species currently included in oil toxicity databases. While the low intensity UVR only significantly increased the negative effects of the oil for one endpoint, the majority of endpoints showed trends towards increased toxicity in the presence of UVR. Therefore, the data presented here further support the standard incorporation of UVR in oil toxicity testing for tropical corals.