Affiner votre recherche
Résultats 1771-1780 de 1,955
Differences between 4-fluoroaniline degradation and autoinducer release by Acinetobacter sp. TW: implications for operating conditions in bacterial bioaugmentation Texte intégral
2013
Wang, Meizhen | Xu, Junjie | Wang, Juehua | Wang, Shuo | Feng, Huajun | Shentu, Jiali | Shen, Dongsheng
To develop a bacterial bioaugmentation system for fluorine-containing industrial wastewater treatment, optimal conditions for 4-fluoroaniline (4-FA) degradation and autoinducer release in Acinetobacter sp. TW were determined. Quorum sensing in biofilms of strain TW was also investigated. Different optimal conditions exist for 4-FA degradation and autoinducer release, particularly with regard to pH. Quorum sensing modulates extracellular polymeric substance (EPS) secretion and biofilm formation in the strain but plays no role in 4-FA degradation. Under optimal conditions for 4-FA degradation, the release of N-3-oxo-hexanoyl-homoserine lactone (3-oxo-C6-HSL) and N-hexanoyl-homoserine lactone (C6-HSL) in strain TW was significantly lower than required for quorum sensing. Under optimal conditions for autoinducer release, on the other hand, 3-oxo-C6-HSL and C6-HSL levels exceeded the quorum sensing thresholds, thereby inducing EPS secretion and biofilm formation. We conclude that the optimal conditions for autoinducer release (25 °C, pH 5, 800 mg L(-1) 4-FA, and 0 % NaCl) are suitable for bacterial colonization in bioaugmentation, while those for 4-FA degradation (25-30 °C, pH 8 and 800 mg L(-1) 4-FA) maximize the system performance after colonization.
Afficher plus [+] Moins [-]Whole cell hybridisation for monitoring harmful marine microalgae Texte intégral
2013
Toebe, Kerstin
Fluorescence in situ hybridisation (FISH) is a powerful molecular biological tool to detect and enumerate harmful microorganism in the marine environment. Different FISH methods are available, and especially in combination with automated counting techniques, the potential for a routine monitoring of harmful marine microalgae is attainable. Various oligonucleotide probes are developed for detecting harmful microalgae. However, FISH-based methods are not yet regularly included in monitoring programmes tracking the presence of harmful marine microalgae. A limitation factor of the FISH technique is the currently available number of suited fluorochromes attached to the FISH probes to detect various harmful species in one environmental sample at a time. However, coupled automated techniques, like flow cytometry or solid-phase cytometry, can facilitate the analysis of numerous field samples and help to overcome this drawback. A great benefit of FISH in contrast to other molecular biological detection methods for harmful marine microalgae is the direct visualisation of the hybridised target cells, which are not permitted in cell free formats, like DNA depending analysis methods. Therefore, an additional validation of the FISH-generated results is simultaneously given.
Afficher plus [+] Moins [-]Spatial distribution of polychlorinated biphenyls in High Tatras lake sediments Texte intégral
2013
van Drooge, Barend L. | Grimalt, Joan O. | Stuchlík, Evzen
The objective of the present study was to gain insight on the spatial distribution of seven polychlorinated biphenyl (PCB) congeners in the High Tatras mountain range in Eastern Europe. Twenty high mountain lakes were sampled on their top-core sediment. Despite the relative uniform composition of PCB congeners among the lakes, there are important differences in the observed concentrations. Moderate top-core sediment concentrations of PCB congeners were observed (1.9 to 38 ng/g dw for ∑PCB) in comparison to other high mountain or Arctic areas. The variation in PCB concentrations can partly be explained by a possible altitudinal effect, resulting in higher PCB concentrations at higher (colder) altitudes. Part of this enhanced accumulation of PCBs could be caused by external factors (topography and meteorology) and internal lake factors (sediment dynamics). Many of these factors were not quantified for all individual lakes and their influence could, therefore, only be studied for some.
Afficher plus [+] Moins [-]Predicting the sensitivity of fishes to dioxin-like compounds: possible role of the aryl hydrocarbon receptor (AhR) ligand binding domain Texte intégral
2013
Doering, Jon A. | Giesy, John P. | Wiseman, Steve | Hecker, Markus
Dioxin-like compounds are chronically toxic to most vertebrates. However, dramatic differences in sensitivity to these chemicals exist both within and among vertebrate classes. A recent study found that in birds, critical amino acid residues in the aryl hydrocarbon receptor (AhR) ligand binding domain are predictive of sensitivity to dioxin-like compounds in a range of species. It is currently unclear whether similar predictive relationships exist for fishes, a group of animals at risk of exposure to dioxin-like compounds. Effects of dioxin-like compounds are mediated through the AhR in fishes and birds. However, AhR dynamics are more complex among fishes. Fishes possess AhRs that can be grouped within at least three distinct clades (AhR1, AhR2, AhR3) with each clade possibly containing multiple isoforms. AhR2 has been shown to be the active form in most teleosts, with AhR1 not binding dioxin-like compounds. The role of AhR3 in dioxin-like toxicity has not been established to date and this clade is only known to be expressed in some cartilaginous fishes. Furthermore, multiple mechanisms of sensitivity to dioxin-like compounds that are not relevant in birds could exist among fishes. Although, at this time, deficiencies exist for the development of such a predictive relationship for application to fishes, successfully establishing such relationships would offer a substantial improvement in assessment of risks of dioxin-like compounds for this class of vertebrates. Elucidation of such relationships would provide a mechanistic foundation for extrapolation among species to allow the identification of the most sensitive fishes, with the ultimate goal of the prediction of risk posed to endangered species that are not easily studied.
Afficher plus [+] Moins [-]The use of low-cost adsorbents for wastewater purification in mining industries Texte intégral
2013
Iakovleva, Evgenia | Sillanpää, Mika
Recently, great attention has been paid to the environmental problems in mining industry. At present there are different ways of mineral processing, as well as various methods of wastewater treatment, most of them are expensive. Work is ongoing to find low-cost treatments. In this article, low-cost adsorbents, potentially useful for wastewater treatment on mining and metallurgical plants, are reviewed; their characteristics, advantages, and disadvantages of their application are compared. Also adsorption of different metals and radioactive compounds from acidic environment similar to composition of mining and metallurgical wastewaters is considered.
Afficher plus [+] Moins [-]The distribution and sea–air transfer of volatile mercury in waste post-desulfurization seawater discharged from a coal-fired power plant Texte intégral
2013
Sun, Lumin | Lin, Shanshan | Feng, Lifeng | Huang, Shuyuan | Yuan, Dongxing
The waste seawater discharged in coastal areas from coal-fired power plants equipped with a seawater desulfurization system might carry pollutants such as mercury from the flue gas into the adjacent seas. However, only very limited impact studies have been carried out. Taking a typical plant in Xiamen as an example, the present study targeted the distribution and sea-air transfer flux of volatile mercury in seawater, in order to trace the fate of the discharged mercury other than into the sediments. Samples from 28 sampling sites were collected in the sea area around two discharge outlets of the plant, daily and seasonally. Total mercury, dissolved gaseous mercury and dissolved total mercury in the seawater, as well as gaseous elemental mercury above the sea surface, were investigated. Mean concentrations of dissolved gaseous mercury and gaseous elemental mercury in the area were 183 and 4.48 ng m(-3) in summer and 116 and 3.92 ng m(-3) in winter, which were significantly higher than those at a reference site. Based on the flux calculation, the transfer of volatile mercury was from the sea surface into the atmosphere, and more than 4.4 kg mercury, accounting for at least 2.2 % of the total discharge amount of the coal-fired power plant in the sampling area (1 km(2)), was emitted to the air annually. This study strongly suggested that besides being deposited into the sediment and diluted with seawater, emission into the atmosphere was an important fate for the mercury from the waste seawater from coal-fired power plants.
Afficher plus [+] Moins [-]Assessment of phytotoxicity of ZnO NPs on a medicinal plant, Fagopyrum esculentum Texte intégral
2013
Lee, Sooyeon | Kim, Sunghyun | Kim, Saeyeon | Lee, Insook
Fagopyrum esculentum commonly named as buckwheat plant is pseudocereal food crops and healthy herbs but is not known as a bioindicator of environmental condition. In the present study, the effects of ZnO nanoparticles (NPs) and microparticles (MPs) on plant growth, bioaccumulation, and antioxidative enzyme activity in buckwheat were estimated under hydroponic culture. The significant biomass reduction at concentrations of 10–2,000 mg/L was 7.7–26.4 % for the ZnO NP and 11.4–23.5 % for the ZnO MP treatment, (p < 0.05). ZnO NPs were observed in root cells and root cell surface by scanning electron microscopy and transmission electron microscopy analysis. Zn bioaccumulation in plant increased with increasing treatment concentrations. The upward translocation (translocation factor <0.2) of Zn in plant was higher with the ZnO NP treatment than that with the ZnO MP treatment. Additionally, reactive oxygen species generation by ZnO NPs was estimated as the reduced glutathione level and catalase activity, which would be a predictive biomarker of nanotoxicity. The results are the first study to evaluate the phytotoxicity of ZnO NPs to medicinal plant. F. esculentum can be as a good indicator of plant species in NP-polluted environment.
Afficher plus [+] Moins [-]Trace metals, anions and polybromodiphenyl ethers in settled indoor dust and their association Texte intégral
2013
Kefeni, Kebede K. | Okonkwo, Jonathan O.
Contaminants in settled indoor dust are potentially health hazardous to human. Thus, identification and quantification of toxic chemicals in settled indoor dust is of great concern. In this study, the levels of major anions ([Formula: see text]), trace metals (Cr, Mn, Fe, Co, Ni, Cu, Zn, Cd, As and Pb) and polybromodiphenyl ethers (PBDEs) in settled office and home dust were determined and correlations between the contaminants investigated. Depending on the available materials in both microenvironments, the most possible sources were identified. The results showed that the settled office dusts (n = 6 pooled samples from 85 offices) were more contaminated than home dusts (n = 8 homes). For anions, [Formula: see text] and Cl(-) accounted for 87 and 97 % of the total office and home dust contaminants, respectively. For trace metals, Fe, Cu, Zn and Mn, accounted for 98 % of the contaminants in both office and home dust samples. Fe exhibited the highest percentage of 76.7 and 87.3 % in office and home dust samples, respectively. For PBDEs, the mean concentrations detected in office and home dust ranged between 5.8-86.3 and 1.5-20.6 ng g(-1), respectively. The log-transformed correlation between the total concentrations of trace metals and major anions detected in offices and homes was positive for offices and negative for homes with a statistically significant values (r = 0.73, p < 0.01; r = -0.22, p < 0.01, respectively). The daily exposure rates determined for the most hazardous such as As, Cd, Pb and PBDEs congeners, relative to the individual concentrations reported in the literature in settled indoor dust, were found very lower. Therefore, maybe it is possible to expect less potential health risk. Investigation of formation of coordination compounds between trace metals and PBDEs congeners is possible; however, this requires further study.
Afficher plus [+] Moins [-]Characterization of extracellular polymeric substances (EPS) from periphyton using liquid chromatography-organic carbon detection–organic nitrogen detection (LC-OCD-OND) Texte intégral
2013
Stewart, Theodora J. | Traber, Jacqueline | Kroll, Alexandra | Behra, Renata | Sigg, Laura
A protocol was developed to extract, fractionate, and quantitatively analyze periphyton extracellular polymeric substances (EPS), which obtains both information on the molecular weight (M ᵣ) distribution and protein and polysaccharide content. The EPS were extracted from freshwater periphyton between July and December 2011. Organic carbon (OC) compounds from different EPS extracts were analyzed using liquid chromatography-organic carbon detection–organic nitrogen detection (LC-OCD-OND), and total protein and polysaccharide content were quantified. Four distinct OC fractions, on the basis of M ᵣ, were identified in all extracts, corresponding to high M ᵣ biopolymers (≥80–4 kDa), degradation products of humic substances (M ᵣ not available), low M ᵣ acids (10–0.7 kDa), and small amphiphilic/neutral compounds (3–0.5 kDa). Low C/N ratios (4.3 ± 0.8) were calculated for the biopolymer fractions, which represented 16–38 % of the measured dissolved organic carbon (DOC), indicating a significant presence of high M ᵣ proteins in the EPS. Protein and polysaccharide represented the two major components of EPS and, when combined, accounted for the measured DOC in extracts. Differences in specific OC fractions of EPS extracts over the course of the study could be quantified using this method. This study suggests that LC-OCD-OND is a new valuable tool in EPS characterization of periphyton.
Afficher plus [+] Moins [-]Effects of copper fungicide residues on the microbial function of vineyard soils Texte intégral
2013
Wightwick, Adam M. | Salzman, Scott A. | Reichman, Suzanne M. | Allinson, Graeme | Menzies, Neal W.
The use of copper-based fungicides leads to an accumulation of copper (Cu) in vineyard soils, potentially causing adverse effects to the microbial function and fertility of the soil. This study used a soil microcosm approach to assess the effects of Cu accumulation on microbial function in vineyard soils. Surface soil samples were collected from 10 vineyards and a number of un-impacted reference sites in each of three different viticultural regions of Australia. The field-collected soils were transferred to microcosms and maintained for up to 93 days in the laboratory at 20–22 °C and 60 % of their maximum water-holding capacity. The microbial function of the soils was indicated by measuring phosphomonoesterase, arylsulfatase, urease, and phenol oxidase activities. In general, the vineyard soils had greater concentrations of Cu and lower enzyme activities than in the reference soils, although a weak negative relationship between Cu and enzyme activity could only be found for phosphomonoesterase activity. The results show that soil physical–chemical properties (i.e., organic carbon, pH) are greater determinants of soil enzyme activity than increased soil Cu concentration at the Cu concentrations present in vineyard soils.
Afficher plus [+] Moins [-]