Potassium hydroxide (KOH) digestion protocols are currently applied to separate microplastics from biological samples, allowing efficient digestion with minor degradation of polymers in a time- and cost-effective way. For biota samples with high-fat content, KOH reacts with triglycerides generating an overlying soap layer, making difficult the digestion and solubilization and subsequent microplastics extraction. Here we studied the addition of Tween-20 in different concentrations to evaluate the effect on the soap layer of post-digested samples. Addition of 10 % of Tween-20 presented higher flow rate during filtration, being set as optimal value. Incorporation of Tween-20 in the extraction procedure increased recovery rates of LDPE, PC and PET and appears to have a protective effect on PC and PET degradation. Tween-20 did not interfere in FTIR spectrum of polymers available in the marine environment. Being low-toxic, makes addition of Tween-20 a simple and economical way to optimize KOH digestion protocols for microplastics extraction.

An unusual coastward expansion of the toxic dinoflagellate species Margalefidinium polykrikoides was observed in 2020 summer after a tropical storm passing Chesapeake Bay. Such coastward expansion was only recorded in 2007. A newly developed coupled Lagrangian particle tracking and harmful algal bloom model driven by environmental variables was used to investigate the underlying mechanisms and successfully reproduced the expansion patterns. Persistent pre-storm southerly winds favored the delivery of bloom source water originated inside the bay to the coast. Storm-induced strong upwelling of denser subsurface water interacted with the after-storm outflow plume (steered southward as the storm's impacts waned), forming a transport barrier to accumulate algae and delineate the coastwide bloom extent. Algal diel vertical migrations and transport barrier enable algae to stay in the nearshore regions. The storm-induced coastward expansion of M. polykrikoides might increase future bloom possibility in the coastal area.

This study focuses on determination of quantity, shape, size and polymer types of microplastics in Pacific oysters (Crassostrea gigas) cultured in Danang Bay, Vietnam. Visual verification and chemical identification of microplastics was carried out by μFT-IR spectra using Nicolet iN10 MX Infrared Imaging Microscope. The average abundance of microplastic in oysters was 1.88 ± 1.58 particles/g (wet weight) and 18.54 ± 10.08 particles/individual. The prevalent shape of microplastics included three main forms as fragment, fiber and bead corresponding to 73.71; 25.84 and 0.45%, respectively. Additionally, the size of microplastics was a range of 22.4–1318.8 μm, and the most common size was less than 100 μm, accounted for 77.30%. Fifteen polymer types were detected while Nylon was the most abundant polymer type with 50.56%. In this study, microplastics accumulation with their abundance and polymeric structures could be considered as reliable evidence for further studies on assessment of their potential risk to human health.

The use of Unmanned Aerial Systems (UAS, aka drones) has shown to be feasible to perform marine litter surveys. We operationally tested the use of multispectral images (5 bands) to classify litter type and material on a beach-dune system. For litter categorization by their multispectral characteristics, the Spectral Angle Mapping (SAM) technique was adopted. The SAM-based categorization of litter agreed with the visual classification, thus multispectral images can be used to fasten and/or making more robust the manual RGB image screening. Fully automated detection returned an F-score of 0.64, and a reasonable categorization of litter. Overall, the image-based litter density maps were in line with the manual detection. Assessments were promising given the complexity of the study area, where different dunes plants and partially-buried items challenged the UAS-based litter detection. The method can be easily implemented for both floating and beached litter, to advance litter survey in the environment.

The current study identifies groundwater quality issues and investigates the most important geochemical processes that control seawater intrusion using various ionic ratios, hydrochemical facies evolution, and geochemical modelling. Cl₋/Br ratio is an important indicator to identify the origin of groundwater salinity in coastal aquifers. Nineteen percent of the groundwater samples with Cl⁻/Br⁻ ratio similar to that of Standard Mean Ocean Water (SMOW) are affected by seawater intrusion in the study area. Particularly, nine groundwater samples have high chloride values and are similar to SMOW, and it may derived salinity from seawater sources from the Bay of Bengal due to the over-pumping of production wells in the Uvari zone. Five samples are similar to SMOW, which is due to the presence of salt pan activities. The bivariate plots such as Ca²⁺ + Mg²⁺ vs Cl⁻, EC vs Cl⁻, and Na⁺/Cl⁻ ratio indicate that seawater intrusion is the primary source for groundwater salinisation. Evaporation is the dominant process controlling groundwater chemistry, rather than rock-water interaction and precipitation, according to mechanisms controlling groundwater chemistry. Direct ion exchange and converse ion exchange are the critical controlling factors for groundwater salinisation, according to the hydrochemical facies evolution diagram (HFED). The water quality index (WQI) shows that most groundwater belongs to the poor to the marginal category. The saturation indices show that the groundwater samples are saturated with minerals such as dolomite, calcite, aragonite and magnesite. Therefore, these minerals are susceptible to precipitation due to the effective leaching of calcareous minerals from the bedrocks. Compiled hydrogeochemical analysis and multivariate statistical analysis revealed that the Tiruchendur and Uvari zone was affected by the seawater intrusion and led to an increase in the salinity of the groundwater.

Exposure risk is assessed based on modeling suitable habitat of large pelagic fish and oil spill scenarios originating at three wells located in the western GM's deep waters. Since the fate of the oil depends on the oceanographic conditions present during the accident, as well as the magnitude and duration of the spill, which are not known a priori, the scenarios used are a statistical representation of the area in which oil spilled from the well could be found, given all possible outcomes. The ecological vulnerability assessment identified a subset of bony fish with low-medium vulnerability and elasmobranchs with medium-high vulnerability. The oiling probability and exposure risk of both bony fish and elasmobranchs hotspots vary by well analyzed. Thus, these results provide essential information for a risk management plan for the assessed species and others with economic or conservation importance distributed in the GM and worldwide.

This study provides the first analysis and quantification of MPs in salt products in Sri Lanka. Commercial table salt brands, rock salt, lab-grade NaCl and raw salt from three salterns were analysed using microscopy and Fourier transform infrared spectroscopy. All salt samples were contaminated with MPs: in commercial salts products it ranged from 11 to 193 items/kg, rock salts had 64 items/kg and lab grade NaCl had 253 ± 8.9 items/kg. The MP levels in salterns varied significantly: Hambantota 3345.7 ± 311.4 items/kg, Puttalam 272.3 ± 10.6 items/kg, and Elephant Pass 36.3 ± 4.5 items/kg. Predominantly, MPs were presented as fibres, followed by fragments. Of the 23 polymer types identified; low-density polyethylene (LDPE; 17%), resin dispersion (15%) and high-density polyethylene (HDPE; 12%) were notable. This study provides the first comparison of MPs in raw salt and commercial salt. This information is important to trace the pollutant sources and then to take steps to eliminate MPs in food products consumed.

Microplastics (MPs) are toxic pollutants, which are found ubiquitously in the marine environment. The present study aimed to assess the level of MP contamination in Vellar estuary, southeast coast of India. MP abundance in surface water and sediments ranged from 1.15 ± 0.01 to 5.14 ± 0.04 items/m³ and 24.8 ± 0.75 to 43.4 ± 0.98 particles/kg d.w., respectively. Fiber, fragment, film, pellet and glitter were present in the study area. In total, ten colors of MPs were found in the study, of which black was the most abundant. Seven polymers in sediments and four polymers in water were characterized from the study area, among which LDPE was the most dominant. Sediment texture results showed that clay particles were able to retain a greater number of MPs. This study shows that the Vellar estuary is contaminated with MPs; however, further investigation is required to classify the estuary as polluted.

Polycyclic aromatic hydrocarbons (PAHs) most likely derived from natural sources were observed in two sediment cores covering the last 100 years in an Amazon estuarine region. A considerable change in the PAHs main source was observed in the 1960s. Before the 1960s, the sources of PAHs seem to be related to biogenic and/or early-diagenetic processes. Concentrations of perylene were higher before the 1960s and suggest that its primary source to the sediments in the Amazon region is linked to a short-term diagenetic transformation of their biogenic precursors. The natural formation of alkylated PAHs in sediments was linked to the methylation of the parental aromatic hydrocarbons due to sediment maturation processes and the dehydrogenation of sterols in the sediments. The relatively rapid reaction occurring in recent sediments of the Amazon region suggests the importance of the microbial community in the transformation of biogenic precursors to alkylated-PAHs in the sediments.

This study investigates the relationships among Ni, Cd and Pb's different chemical forms determined by different methodologies in coastal and transitional waters across a broad geographical scale. Concentrations were measured in spot samples and through passive sampling (DGT). High variability of metal concentrations was found among sampling sites and methodologies due to natural water fluctuations rather than to a given metal or method. Total dissolved metal concentrations in spot samples were lower than the EQS-WFD values. The labile fractions of Cd and Pb, measured in spot samples by Anodic Stripping Voltammetry and by DGT-ICPMS, were highly correlated. Similar labilities were found for Cd, while for Pb, the ASV labile fraction was ≈50% lower. These results reflect the pool of mobile and labile species available towards each technique kinetic window, and they seem not to be affected by discrete sampling flaws.