Antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARB) have become a critical global public health issue in this century. There is increasing evidence for the presence and transmission of ARGs by air transmission. In this research, ARGs and ARB in air conditioner filter dust (AC dust) and urine samples from 55 kindergarten children in 17 kindergartens and nearby 10 soil samples in Hong Kong were analyzed. The results showed the presence of 16 ARG subtypes and the mobile genetic element (MGE) intI1 in AC dust, and 12 ARG subtypes in the soil samples. ARGs presenting resistance to sulfonamide (6.9 × 10⁻³–0.17) (expressed as relative abundance of the 16 S rRNA genes) were most abundant followed by macrolides (1.8 × 10⁻³–3.3 × 10⁻²), sul1, sul2 (sulfonamide), ermF (macrolides) and intI1 genes in AC dust in 17 kindergartens. For soil samples, 12 ARG subtypes and the intI1 were detected, and the genes providing resistance to sulfonamide (1.6 × 10⁻³–2.7 × 10⁻¹) were the most abundant ARGs in the 10 soil samples, followed by tetracycline (ND–1.4 × 10⁻²). Multi-resistant bacteria with sul1, sul2, intI1, or tetQ were detected in all AC dust samples and some urine samples. Based on bacterial genera and ARG co-occurrence network analysis and Hong Kong’s special geographical location and cultural environment, there might be two origins for the ARGs detected in the kindergartens: β-lactam/macrolide ARGs mainly derived from human medicine use and tetracycline/sulfonamide ARGs mainly from other areas, as well as IntI1 may play a role in the spread of ARGs in Hong Kong. The widely detection of ARGs in AC dust in kindergartens in Hong Kong highlights the need for the improvement of management measures.

Fourteen perfluoroalkyl substances (PFASs) in fishery organism, surface seawater, river water, rainwater, and wastewater samples collected from Jiaozhou Bay (JZB) in China and its surrounding area were determined to understand their contamination status, sources, health risk, and causes of spatiotemporal variations in the aquatic environment of a temperate bay adjacent to a metropolis. The total concentration of PFASs in 14 species of fishery organisms ranged from 1.77 ng/g to 31.09 ng/g wet weight, and perfluorooctane sulfonate (PFOS) was the dominant PFAS. ∑PFASs concentration in surface seawater ranged from 5.54 ng/L to 48.27 ng/L over four seasons, and dry season (winter and spring) had higher levels than wet season (summer and autumn). Perfluorooctanoic acid (PFOA) was the predominant individual PFAS in seawater, indicating that notorious C8 homologs remained the major PFASs in this region. The seasonal variation in seawater concentrations of three major PFASs, namely, PFOA, perfluoroheptanoic acid, and perfluorononanoic acid, was similar to that of ∑PFASs. However, the seasonal variation of PFOS concentration was different from that of ∑PFASs, with the lowest in winter and the highest in spring. In general, seasonal variations of terrigenous input and water exchange capacity were the main reasons for the spatiotemporal variation of PFASs in the aquatic environment of JZB. Moreover, bioselective enrichment for individual PFAS affected the partition of PFASs in different environment medium. Wet precipitation, sewage discharge, and surface runoff were the main sources of PFASs in this area. Nevertheless, the contribution of different sources to individual PFAS indicated a clear difference, and wastewater and river water were not consistently the most important source for every PFAS. Preliminary risk assessment revealed that the consumption of seafood, especially fish, from JZB might pose a certain extent of health risk to local consumers based on their estimated daily intake of PFASs.

The abnormality in thyroid hormone modulation in developmental fish, vulnerable to per- and polyfluorinated substances, is of particular concerns for the alternative substances. Juvenile rare minnows, were exposed to chlorinated polyfluoroalkyl ether sulfonates (Cl-PFESAs), the novel alternatives to perfluorooctane sulfonate (PFOS), for 4 weeks followed by 12 weeks of depuration. Half lives were determined to be 33 d, 29 d, and 47 d for total Cl-PFESAs, C8 Cl-PFESA and C10 Cl-PFESA, respectively. Preliminary toxicity test suggested that Cl-PFESAs are moderately toxic to Rare minnow with a LC50 of 20.8 mg/L (nominal concentration） after 96 h of exposure. In the chronic toxicity test, fishes were exposed to Cl-PFESAs at geometric mean measured concentrations of 86.5 μg/L, 162 μg/L and 329 μg/L. In juvenile fishes exposed to Cl-PFESAs for 4 weeks, gene profile sequencing analysis identified 3313 differentially expressed genes, based on which pathways regulating thyroid hormone synthesis and steroid synthesis were enriched. Both whole body total and free 3,5,3′-triiodothyronine (T3) levels were significantly increased. mRNA expression of genes regulating thyroid hormone synthesis (corticotropin-releasing hormone (CRH), thyroid-stimulating hormone (THS), sodium/iodide symporter (NIS), thyroglobulin (TG), and thyroid peroxidase (TPO), transport (transthyretin,TTR), deiodinase (Dio1, Dio2) and receptor (TRα and TRβ) were decreased. Uridinediphosphate glucoronosyl-transferases (UGT1A) gene, regulating THs metabolism, was also decreased. In adult fish, thyroid hormone and genes expression in hypothalamic-pituitary-thyroid axis remained at disturbed levels after 12 weeks of depuration without exposure. Chronic developmental exposure to Cl-PFESAs caused persistent thyroid hormone disrupting effects in fish, highlighting a necessity of comprehensive ecological risk assessment.

Evidence of health effects following early life exposure to short-to-medium duration of high pollution levels is extremely limited.We aimed to evaluate the associations between: 1. intrauterine exposure to fine particulate matter (PM2.5) from coal mine fire emissions and the frequencies of general practitioner attendances and dispensations of prescribed asthma inhalers, steroid skin creams, and antibiotics during the first year of life; 2. infant exposure and those outcomes during the year following the fire.All participants were recruited from the Latrobe Valley of Victoria, Australia. Participants’ 24-h average and hourly peak mine fire-specific PM2.5 exposures from 09/02/2014 to 31/03/2014 were estimated using chemical transport modelling. Outcome data were obtained from the Australian Medicare Benefits Schedule and Pharmaceutical Benefits Scheme from each child’s birth to 31/12/2016. We used negative binomial and logistic regression models to independently assess risks of the outcomes associated with every 10 and 100 μg m−3 increase in average or peak PM2.5 exposure, respectively, while adjusting for potential confounders.We included 286 of 311 children whose parents consented to be linked, comprising 77 with no exposure, 88 with intrauterine exposure and 121 with exposure in infancy. 10- and 100- μg m−3 increases in average and peak PM2.5 exposure during infancy were associated with greater incidence of antibiotics being dispensed during the year following the fire: the adjusted incidence rate ratios were 1.24 (95% CI 1.02, 1.50, p = 0.036) and 1.14 (1.00, 1.31, p = 0.048) respectively. No other significant associations were observed.Exposure to coal mine fire emissions during infancy was associated with increased dispensing of antibiotics. This could reflect increased childhood infections or increased prescriptions of antibiotics in the year following the fire.

Nano-magnetite supported by biochar (nFe₃O₄/BC) pyrolyzed at temperatures of 300 °C–600 °C was developed to activate hydrogen peroxide (H₂O₂) for the efficient degradation of ethylbenzene in aqueous solution. It was revealed that the degradation efficiency of ethylbenzene and TOC removal were 96.9% and 36.2% respectively after the reaction for 40 min in the presence of initial concentration of 0.1 mmol L⁻¹ ethylbenzene, 2.76 g L⁻¹ nFe₃O₄/BC₅₀₀ with the mass ratio of nFe₃O₄ to BC₅₀₀ of 4:1 and 2.0 mmol L⁻¹ H₂O₂ at pH 7.0. Based on electron paramagnetic resonance (EPR), quenching experiment and X-ray photoelectron spectroscopy (XPS) data, both OH and O₂⁻ radicals were generated in the nFe₃O₄/BC₅₀₀ activated H₂O₂ system, and the OH radicals were the predominant species for the degradation of ethylbenzene. Through electron transfer process, mechanisms of Fe(II), phenolic hydroxyl group and persistent free radicals (PFRs) on BC surfaces accounted for the generation of OH radicals, and Fe(III) in nFe₃O₄ and formed from Fe(II) oxidation responsible for the generation of O₂⁻ radicals in the nFe₃O₄/BC activated H₂O₂ system were proposed.

The 2011 spill at platforms B and C of the Penglai 19-3 oil field in the Bohai Sea has been the worst oil spill accident in China. To assess long-term effects, a comprehensive monitoring program of chemical and biological variables (within a 2.2 km radius of the spill site) was conducted five years after the spill. Comparison of nutrient, Chl-a and oil concentrations in seawater, TOC, PAHs, heavy metals concentrations within the sediments, and the abundance and biomass of macrobenthic organisms to values obtained before and after the oil spill in previous studies indicate habitat recovery has occurred within the Bohai Sea following the episodic oil release. Observed elevated oil concentration in the water column and higher concentrations of two heavy metals, five PAHs, TOC, TOC/TN and lower values of δ¹³C, together with a reduction in macrobenthic biomass in near-field samples, suggest the influence of contaminants from chronic releases of oil and operational waste discharges within the vicinity of the oil platforms.

Despite the mercury (Hg) control measures adopted by the international community, Hg still poses a significant risk to ecosystem and human health. This is primarily due to the ability of atmospheric Hg to travel intercontinentally and contaminating terrestrial and aquatic environments far from its natural and anthropogenic point sources. The issue of Hg pollution is further complicated by its unique physicochemical characteristics, most noticeably its multiple chemical forms that vary in their toxicity and environmental mobility. This meant that most of the risk evaluation protocols developed for other metal(loid)s are not suitable for Hg. Soil is a major reservoir of Hg and a key player in its global cycle. To fully assess the risks of soil Hg it is essential to estimate its bioavailability and/or availability which are closely linked to its toxicity. However, the accurate determination of the (bio)-available pools of Hg in soils is problematic, because the terms ‘bioavailable’ and ‘available’ are ill-defined. In particular, the term ‘bioavailable pool’, representing the fraction of Hg that is accessible to living organisms, has been consistently misused by interchanging with other intrinsically different terms e.g. mobile, labile, reactive and soluble pools. A wide array of physical, chemical, biological and isotopic exchange methods were developed to estimate the (bio)-available pools of Hg in soil in an attempt to offer a plausible assessment of its risks. Unfortunately, many of these methods do not mirror the (bio)-available pools of soil Hg and suffer from technical drawbacks. In this review, we discuss advantages and disadvantages of methods that are currently applied to quantify the (bio)-availability of Hg in soils. We recommended the most feasible methods and give suggestions how to improve the determination of (bio)-available Hg in soils.

Heavy metal contamination in protected-field vegetable production has aroused widespread concern and manure is considered to be one of the contamination sources. Little is known about its long-term effects on heavy metal pollution in uncontaminated soils. A 15-year protected-field vegetable production experiment was carried out with three manure treatments (chicken manure: cattle manure = 3:1) with high (HMAR), medium (MMAR) and low (LMAR) application rates to evaluate the long-term risks of heavy metal pollution. It was found that continuous and high manure application rates significantly increased the total concentrations of soil Cd, Zn, Cr, and Cu rather than Pb, Ni or As. The high application rate of manure also increased soil available heavy metals although the soil organic matter was increased as well. Though total soil Cd under the HMAR exceeded the threshold of national soil standard, Cd content in tomato and fennel still complied with the food safety requirements of vegetables. Generally, the accumulation rates of soil Zn, Cu, and Cr with 1 t⋅ha⁻¹ of manure application in three treatments were ranked by HMAR < MMAR < LMAR. Based on the results of the ratio of heavy metal accumulation risk (RAR), Zn, Cu, and Cr under HMAR and Cd and Zn under MMAR would exceed their soil threshold values within 100 years and RAR could be a useful indicator for monitoring the long-term risk of soil heavy metal pollution. Recommended manure application rates to guarantee a 100-year period of clean production were 44, 74, and 63 t⋅ha⁻¹⋅yr⁻¹ for Zn, Cu, and Cr, respectively. Measurements should be taken to minimize the risk of heavy metals (Cd, Zn, Cr, and Cu) pollution sourced from manure to ensure food safety and ‘cleaner’ protected-field vegetable production.

The use of pesticides has historically helped improve agricultural productivity, although their continued use may have unforeseen effects upon the natural environment when not applied appropriately. Metaldehyde is a commercial pesticide widely used to reduce crop losses resulting from terrestrial mollusc damage. However, following precipitation and runoff it frequently enters waterbodies with largely unknown consequences for aquatic fauna. This study represents one of the first attempts to examine its potential effects on aquatic macroinvertebrate communities at sites known to have experienced elevated metaldehyde concentrations alongside unaffected control sites. In addition, a series of laboratory exposures specifically examined the effects of metaldehyde on the survivorship of non-target aquatic mollusc species. When the entire aquatic macroinvertebrate community and aquatic mollusc community were considered, limited differences were observed between metaldehyde affected and control sites based on field data. Laboratory exposures highlighted that for the molluscs examined, gastropods (Bithynia tentaculata, Planorbis planorbis, Radix balthica and Potamopyrgus antipodarum) had a greater tolerance to metaldehyde than bivalves (Sphaerium corneum and Corbicula fluminea). However, the concentrations required to reduce survivorship of all species were much greater than those ever recorded historically under field conditions. The results suggest that the differences in the community composition recorded between sites exposed to elevated metaldehyde concentrations and control sites were probably due to nutrient loading (N and P from agricultural fertilizers) rather than metaldehyde. However, these results do not negate wider concerns regarding metaldehyde use, particularly issues caused when ingested by vertebrate wildlife, livestock or children and pets in domestic settings.

In this study, size-fractionated particulate matters (PM) down to ultrafine (PM₀.₁) particles were collected using a cascade air sampler with a PM₀.₁ stage, in Hat Yai city, Songkhla province, southern Thailand during the year 2018. The particle-bound carbonaceous aerosols (CA) as elemental carbon (EC) and organic carbon (OC) were quantified with the thermal/optical reflectance method following the IMPROVE_TOR protocol. The concentrations of different temperature carbon fractions (OC1-OC4, EC1-EC3 and PyO) in the size-fractionated PM were evaluated to discern OC and EC correlations as well as those between char-EC and soot-EC. The results showed that biomass burning, motor vehicle, and secondary organic aerosols (SOC) all contributed to the size-fractionated PM. The OC/EC ratios ranged from 2.90 to 4.30 over the year, with the ratios of PM₂.₅₋₁₀ being the highest, except during the open biomass burning period. The concentration of CA was found to increase during the pre-monsoon season and had its peak value in the PM₀.₅₋₁.₀ fraction. The long-range transport of PMs from Indonesia, southwest of Thailand toward southern Thailand became more obvious during the pre-monsoon season. Transported plumes from biomass burning in Indonesia may increase the concentration of OC and EC both in the fine (PM₀.₅₋₁.₀ and PM₁.₀₋₂.₅) and coarse (PM₂.₅₋₁₀ and PM>₁₀) fractions. The OC fraction in PM₀.₁ was also shown to be significantly affected by the transported plumes during the pre-monsoon season. Good OC and EC correlations (R² = 0.824–0.915) in the fine particle fractions indicated that they had common sources such as fossil fuel combustion. However, the lower and moderate correlations (R² = 0.093–0.678) among the coarser particles suggesting that they have a more complex pattern of emission sources during the dry and monsoon seasons. This indicates the importance of focusing emission control strategies on different PM particle sizes in southern Thailand.